S1P1/EDG-1 Antibody (713412) [Unconjugated]

Images

 
HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG‑1 and GFP was stained with (A) Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A isotype control antibody ...read more
Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A Isotype ...read more
S1P1/EDG‑1 was detected in perfusion fixed frozen sections of mouse heart using Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) at 25 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clone
713412
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

S1P1/EDG-1 Antibody (713412) [Unconjugated] Summary

Immunogen
Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
Accession # O08530
Specificity
Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
S1PR1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB7089 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S1P1/EDG-1 Antibody (713412) [Unconjugated]

  • CD363 antigen
  • CD363
  • CHEDG1
  • D1S3362
  • EDG1
  • EDG-1
  • EDG1edg-1
  • Endothelial differentiation G-protein coupled receptor 1
  • endothelial differentiation, sphingolipid G-protein-coupled receptor, 1
  • FLJ58121
  • S1P receptor 1
  • S1P receptor Edg-1
  • S1P1
  • S1P1ECGF1
  • S1PR1
  • sphingosine 1-phosphate receptor 1
  • sphingosine 1-phosphate receptor EDG1
  • Sphingosine 1-phosphate receptor Edg-1
  • sphingosine-1-phosphate receptor 1

Background

S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S1P1/EDG-1 Antibody (MAB7089)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(22)
All Applications
Filter By Species
Human
(1)
Mouse
(21)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using MAB7089 Applications Species
J Nie, AC Carpenter, LB Chopp, T Chen, M Balmaceno-, T Ciucci, Q Xiao, MC Kelly, DB McGavern, Y Belkaid, R Bosselut The transcription factor LRF promotes integrin beta7 expression by and gut homing of CD8alphaalpha+ intraepithelial lymphocyte precursors Nature Immunology, 2022;23(4):594-604. 2022 [PMID: 35354951] (Flow Cytometry, Mouse) Flow Cytometry Mouse
S Tsiomita, EM Liveri, P Vardaka, A Vogiatzi, A Skiadaresi, G Saridis, I Tsigkas, TM Michaelidi, G Mavrothala, G Thyphronit ETS2 repressor factor (ERF) is involved in T lymphocyte maturation acting as regulator of thymocyte lineage commitment Journal of leukocyte biology, 2022;0(0):. 2022 [PMID: 35258130] (Flow Cytometry, Human) Flow Cytometry Human
C Liu, L Ma, Y Wang, J Zhao, P Chen, X Chen, Y Wang, Y Hu, Y Liu, X Jia, Z Yang, X Yin, J Wu, S Wu, H Zheng, X Ma, X Sun, Y He, L Lin, Y Fu, K Liao, X Zhou, S Jiang, G Fu, J Tang, W Han, XL Chen, W Fan, Y Hong, J Han, X Huang, BA Li, N Xiao, C Xiao, G Fu, WH Liu Glycogen synthase kinase 3 drives thymocyte egress by suppressing beta-catenin activation of Akt Science Advances, 2021;7(41):eabg6262. 2021 [PMID: 34623920] (Flow Cytometry, Mouse) Flow Cytometry Mouse
M Okuniewska, V Fang, A Baeyens, V Raghavan, JY Lee, DR Littman, SR Schwab SPNS2 enables T�cell egress from lymph nodes during an immune response Cell Reports, 2021;36(2):109368. 2021 [PMID: 34260944] (Flow Cytometry, Mouse) Flow Cytometry Mouse
A Baeyens, S Bracero, VS Chaluvadi, A Khodadadi-, M Cammer, SR Schwab Monocyte-derived S1P in the lymph node regulates immune responses Nature, 2021;0(0):. 2021 [PMID: 33658712] (Flow Cytometry, Mouse) Flow Cytometry Mouse
R Vallion, J Divoux, S Glauzy, E Ronin, Y Lombardi, M Lubrano di, S Grégoire, I Nemazanyy, A Durand, D Fradin, B Lucas, BL Salomon Regulatory T Cell Stability and Migration Are Dependent on mTOR J. Immunol., 2020;0(0):. 2020 [PMID: 32839235] (Flow Cytometry, Mouse) Flow Cytometry Mouse
MJ McCarron, M Irla, A Sergé, SM Soudja, JC Marie Transforming Growth Factor-beta signaling in alphabeta thymocytes promotes negative selection Nat Commun, 2019;10(1):5690. 2019 [PMID: 31857584] (Flow Cytometry, Mouse) Flow Cytometry Mouse
K Shimano, Y Maeda, H Kataoka, M Murase, S Mochizuki, H Utsumi, K Oshita, K Sugahara Amiselimod (MT-1303), a novel sphingosine 1-phosphate receptor-1 functional antagonist, inhibits progress of chronic colitis induced by transfer of CD4+CD45RBhigh�T cells PLoS ONE, 2019;14(12):e0226154. 2019 [PMID: 31805144] (Flow Cytometry, Mouse) Flow Cytometry Mouse
J Choi, PT Rudak, S Lesage, SMM Haeryfar Glycolipid Stimulation of Invariant NKT Cells Expands a Unique Tissue-Resident Population of Precursors to Mature NK Cells Endowed with Oncolytic and Antimetastatic Properties J. Immunol., 2019;0(0):. 2019 [PMID: 31462506] (Flow Cytometry, Mouse) Flow Cytometry Mouse
MM Moretto, S Hwang, K Chen, IA Khan Complex and Multilayered Role of IL-21 Signaling during Thymic Development J. Immunol., 2019;0(0):. 2019 [PMID: 31341076] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Show All 22 Publications.

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Bioinformatics

Gene Symbol S1PR1
Uniprot