S1P1/EDG-1 Antibody (713412) [Unconjugated]

Images

 
HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG‑1 and GFP was stained with (A) Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A isotype control antibody ...read more
S1P1/EDG‑1 was detected in perfusion fixed frozen sections of mouse heart using Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) at 25 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A Isotype ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clone
713412
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

S1P1/EDG-1 Antibody (713412) [Unconjugated] Summary

Immunogen
Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
Accession # O08530
Specificity
Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.
Details of Functionality
ActivityAssaywCitation not found None None
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
S1PR1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-ready
Publications
Read Publications using
MAB7089 in the following applications:

  • 10 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S1P1/EDG-1 Antibody (713412) [Unconjugated]

  • CD363 antigen
  • CD363
  • CHEDG1
  • D1S3362
  • EDG1
  • EDG-1
  • EDG1edg-1
  • Endothelial differentiation G-protein coupled receptor 1
  • endothelial differentiation, sphingolipid G-protein-coupled receptor, 1
  • FLJ58121
  • S1P receptor 1
  • S1P receptor Edg-1
  • S1P1
  • S1P1ECGF1
  • S1PR1
  • sphingosine 1-phosphate receptor 1
  • sphingosine 1-phosphate receptor EDG1
  • Sphingosine 1-phosphate receptor Edg-1
  • sphingosine-1-phosphate receptor 1

Background

S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S1P1/EDG-1 Antibody (MAB7089)(10)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow.


Filter By Application
Flow
(10)
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Filter By Species
Mouse
(10)
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Showing Publications 1 - 10 of 10.
Publications using MAB7089 Applications Species
MJ Lang, M Mori, J Ruer-Laven, J Pieters A Coronin 1-Dependent Decision Switch in Juvenile Mice Determines the Population of the Peripheral Naive T Cell Compartment J. Immunol., 2017;0(0):. 2017 [PMID: 28821585] (Flow,, Mouse) Flow Mouse
A Audemard-V, M Rivière, A Durand, E Peranzoni, V Guichard, P Hamon, N Bonilla, T Guilbert, A Boissonnas, C Auffray, G Eberl, B Lucas, B Martin Macrophages Induce Long-Term Trapping of ?? T Cells with Innate-like Properties within Secondary Lymphoid Organs in the Steady State J. Immunol., 2017;0(0):. 2017 [PMID: 28779024] (Flow,, Mouse) Flow Mouse
A Mendoza, V Fang, C Chen, M Serasinghe, A Verma, J Muller, VS Chaluvadi, ML Dustin, T Hla, O Elemento, JE Chipuk, SR Schwab Lymphatic endothelial S1P promotes mitochondrial function and survival in naive T cells Nature, 2017;0(0):. 2017 [PMID: 28538737] (Flow,, Mouse) Flow Mouse
R Ruscher, RL Kummer, YJ Lee, SC Jameson, KA Hogquist CD8?? intraepithelial lymphocytes arise from two main thymic precursors Nat. Immunol., 2017;0(0):. 2017 [PMID: 28530714] (Flow,, Mouse) Flow Mouse
Maeda Y, Seki N, Kataoka H, Takemoto K, Utsumi H, Fukunari A, Sugahara K, Chiba K IL-17-Producing Vgamma4+ gammadelta T Cells Require Sphingosine 1-Phosphate Receptor 1 for Their Egress from the Lymph Nodes under Homeostatic and Inflammatory Conditions. J Immunol, 2015;195(4):1408-16. 2015 [PMID: 26170380] (Flow,, Mouse) Flow Mouse
Xu, Shengli, Ou, Xijun, Huo, Jianxin, Lim, Kristen, Huang, Yuhan, Chee, Sheena, Lam, Kong-Pen Mir-17-92 regulates bone marrow homing of plasma cells and production of immunoglobulin G2c. Nat Commun, 2015;6(0):6764. 2015 [PMID: 25881561] (Flow,, Mouse) Flow Mouse
Nakai A, Hayano Y, Furuta F, Noda M, Suzuki K Control of lymphocyte egress from lymph nodes through beta2-adrenergic receptors. J Exp Med, 2014;211(13):2583-98. 2014 [PMID: 25422496] (Flow,, Mouse) Flow Mouse
Nomachi, Akira, Yoshinaga, Masanori, Liu, Jaron, Kanchanawong, Pakorn, Tohyama, Kiyoshi, Thumkeo, Dean, Watanabe, Takeshi, Narumiya, Shuh, Hirata, Takako Moesin controls clathrin-mediated S1PR1 internalization in T cells. PLoS ONE, 2013;8(12):e82590. 2013 [PMID: 24358210] (Flow,, Mouse) Flow Mouse
Arnon, Tal I, Horton, Robert M, Grigorova, Irina L, Cyster, Jason G Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress. Nature, 2013;493(7434):684-8. 2013 [PMID: 23263181] (Flow,, Mouse) Flow Mouse
Green JA, Suzuki K, Cho B, Willison LD, Palmer D, Allen CD, Schmidt TH, Xu Y, Proia RL, Coughlin SR, Cyster JG The sphingosine 1-phosphate receptor S1P(2) maintains the homeostasis of germinal center B cells and promotes niche confinement. Nat. Immunol., 2011;12(7):672-80. 2011 [PMID: 21642988] (Flow,, Mouse) Flow Mouse

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Bioinformatics

Gene Symbol S1PR1
Entrez
Uniprot