S1P1/EDG-1 Antibody (713412) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG‑1 and GFP was stained with (A) Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A isotype control antibody (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

S1P1/EDG‑1 was detected in perfusion fixed frozen sections of mouse heart using Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter­stained with hematoxylin (blue). Specific staining was localized to endothelial cells in capillaries. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 713412
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

S1P1/EDG-1 Antibody (713412) [Unconjugated] Summary

• Immunogen: Mouse S1P₁/EDG‑1 synthetic peptide (T4-H28)
  Accession # O08530
• Specificity: Detects mouse S1P₁/EDG‑1 peptide in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: S1PR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S1P1/EDG-1 Antibody (713412) [Unconjugated]

• CD363 antigen
• CD363
• CHEDG1
• D1S3362
• EDG1
• EDG-1
• EDG1edg-1
• Endothelial differentiation G-protein coupled receptor 1
• endothelial differentiation, sphingolipid G-protein-coupled receptor, 1
• FLJ58121
• S1P receptor 1
• S1P receptor Edg-1
• S1P1
• S1P1ECGF1
• S1PR1
• sphingosine 1-phosphate receptor 1
• sphingosine 1-phosphate receptor EDG1
• Sphingosine 1-phosphate receptor Edg-1
• sphingosine-1-phosphate receptor 1

Background

S1P₁ (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P₁ is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P₁ share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S1P1/EDG-1 Antibody (MAB7089)(24)

Publications using MAB7089

• DB Blanco, NM Chapman, JL Raynor, C Xu, W Su, A Kc, W Li, SA Lim, S Schattgen, H Shi, I Risch, Y Sun, Y Dhungana, Y Kim, J Wei, S Rankin, G Neale, PG Thomas, K Yang, H Chi PTEN? directs developmental and metabolic signaling for innate-like T cell fate and tissue homeostasis Nature Cell Biology, 2022-10-27;24(11):1642-1654. 2022-10-27 [PMID: 36302969] (Flow Cytometry, Mouse)
• J Nie, AC Carpenter, LB Chopp, T Chen, M Balmaceno-, T Ciucci, Q Xiao, MC Kelly, DB McGavern, Y Belkaid, R Bosselut The transcription factor LRF promotes integrin beta7 expression by and gut homing of CD8alphaalpha+ intraepithelial lymphocyte precursors Nature Immunology, 2022-03-30;23(4):594-604. 2022-03-30 [PMID: 35354951] (Flow Cytometry, Mouse)
• S Tsiomita, EM Liveri, P Vardaka, A Vogiatzi, A Skiadaresi, G Saridis, I Tsigkas, TM Michaelidi, G Mavrothala, G Thyphronit ETS2 repressor factor (ERF) is involved in T lymphocyte maturation acting as regulator of thymocyte lineage commitment Journal of leukocyte biology, 2022-03-08;0(0):. 2022-03-08 [PMID: 35258130] (Flow Cytometry, Human)
• C Liu, L Ma, Y Wang, J Zhao, P Chen, X Chen, Y Wang, Y Hu, Y Liu, X Jia, Z Yang, X Yin, J Wu, S Wu, H Zheng, X Ma, X Sun, Y He, L Lin, Y Fu, K Liao, X Zhou, S Jiang, G Fu, J Tang, W Han, XL Chen, W Fan, Y Hong, J Han, X Huang, BA Li, N Xiao, C Xiao, G Fu, WH Liu Glycogen synthase kinase 3 drives thymocyte egress by suppressing beta-catenin activation of Akt Science Advances, 2021-10-08;7(41):eabg6262. 2021-10-08 [PMID: 34623920] (Flow Cytometry, Mouse)
• M Okuniewska, V Fang, A Baeyens, V Raghavan, JY Lee, DR Littman, SR Schwab SPNS2 enables T�cell egress from lymph nodes during an immune response Cell Reports, 2021-07-13;36(2):109368. 2021-07-13 [PMID: 34260944] (Flow Cytometry, Mouse)
• A Baeyens, S Bracero, VS Chaluvadi, A Khodadadi-, M Cammer, SR Schwab Monocyte-derived S1P in the lymph node regulates immune responses Nature, 2021-03-03;0(0):. 2021-03-03 [PMID: 33658712] (Flow Cytometry, Mouse)
• R Vallion, J Divoux, S Glauzy, E Ronin, Y Lombardi, M Lubrano di, S Grégoire, I Nemazanyy, A Durand, D Fradin, B Lucas, BL Salomon Regulatory T Cell Stability and Migration Are Dependent on mTOR J. Immunol., 2020-08-24;0(0):. 2020-08-24 [PMID: 32839235] (Flow Cytometry, Mouse)
• X Geng, K Yanagida, RG Akwii, D Choi, L Chen, Y Ho, B Cha, MR Mahamud, K Berman de, H Ichise, H Chen, J Wythe, CM Mikelis, T Hla, RS Srinivasan S1PR1 regulates the quiescence of lymphatic vessels by inhibiting laminar shear stress-dependent VEGF-C signaling JCI Insight, 2020-07-23;0(0):. 2020-07-23 [PMID: 32544090] (IHC, Transgenic Mouse)
• MJ McCarron, M Irla, A Sergé, SM Soudja, JC Marie Transforming Growth Factor-beta signaling in alphabeta thymocytes promotes negative selection Nat Commun, 2019-12-19;10(1):5690. 2019-12-19 [PMID: 31857584] (Flow Cytometry, Mouse)
• K Shimano, Y Maeda, H Kataoka, M Murase, S Mochizuki, H Utsumi, K Oshita, K Sugahara Amiselimod (MT-1303), a novel sphingosine 1-phosphate receptor-1 functional antagonist, inhibits progress of chronic colitis induced by transfer of CD4+CD45RBhigh�T cells PLoS ONE, 2019-12-05;14(12):e0226154. 2019-12-05 [PMID: 31805144] (Flow Cytometry, Mouse)
• J Choi, PT Rudak, S Lesage, SMM Haeryfar Glycolipid Stimulation of Invariant NKT Cells Expands a Unique Tissue-Resident Population of Precursors to Mature NK Cells Endowed with Oncolytic and Antimetastatic Properties J. Immunol., 2019-08-28;0(0):. 2019-08-28 [PMID: 31462506] (Flow Cytometry, Mouse)
• MM Moretto, S Hwang, K Chen, IA Khan Complex and Multilayered Role of IL-21 Signaling during Thymic Development J. Immunol., 2019-07-24;0(0):. 2019-07-24 [PMID: 31341076] (Flow Cytometry, Mouse)
• MJ Lang, M Mori, J Ruer-Laven, J Pieters A Coronin 1-Dependent Decision Switch in Juvenile Mice Determines the Population of the Peripheral Naive T Cell Compartment J. Immunol., 2017-08-18;0(0):. 2017-08-18 [PMID: 28821585] (Flow Cytometry, Mouse)
• A Audemard-V, M Rivière, A Durand, E Peranzoni, V Guichard, P Hamon, N Bonilla, T Guilbert, A Boissonnas, C Auffray, G Eberl, B Lucas, B Martin Macrophages Induce Long-Term Trapping of ?? T Cells with Innate-like Properties within Secondary Lymphoid Organs in the Steady State J. Immunol., 2017-08-04;0(0):. 2017-08-04 [PMID: 28779024] (Flow Cytometry, Mouse)
• DP Golec, RE Hoeppli, LM Henao Cavi, J McCann, MK Levings, TA Baldwin Thymic progenitors of TCR??(+) CD8?? intestinal intraepithelial lymphocytes require RasGRP1 for development J. Exp. Med., 2017-06-26;0(0):. 2017-06-26 [PMID: 28652304] (Flow Cytometry, Mouse)
• A Mendoza, V Fang, C Chen, M Serasinghe, A Verma, J Muller, VS Chaluvadi, ML Dustin, T Hla, O Elemento, JE Chipuk, SR Schwab Lymphatic endothelial S1P promotes mitochondrial function and survival in naive T cells Nature, 2017-05-24;0(0):. 2017-05-24 [PMID: 28538737] (Flow Cytometry, Mouse)
• R Ruscher, RL Kummer, YJ Lee, SC Jameson, KA Hogquist CD8?? intraepithelial lymphocytes arise from two main thymic precursors Nat. Immunol., 2017-05-22;0(0):. 2017-05-22 [PMID: 28530714] (Flow Cytometry, Mouse)
• VM Turner, NA Mabbott Ageing adversely affects the migration and function of marginal zone B cells Immunology, 2017-05-04;151(3):349-362. 2017-05-04 [PMID: 28369800] (Flow Cytometry, Mouse)
• Maeda Y, Seki N, Kataoka H, Takemoto K, Utsumi H, Fukunari A, Sugahara K, Chiba K IL-17-Producing Vgamma4+ gammadelta T Cells Require Sphingosine 1-Phosphate Receptor 1 for Their Egress from the Lymph Nodes under Homeostatic and Inflammatory Conditions. J Immunol, 2015-07-13;195(4):1408-16. 2015-07-13 [PMID: 26170380] (Flow Cytometry, Mouse)
• Xu , Shengli, Ou , Xijun, Huo , Jianxin, Lim , Kristen, Huang , Yuhan, Chee , Sheena, Lam , Kong-Pen Mir-17-92 regulates bone marrow homing of plasma cells and production of immunoglobulin G2c. Nat Commun, 2015-04-17;6(0):6764. 2015-04-17 [PMID: 25881561] (Flow Cytometry, Mouse)
• Nakai A, Hayano Y, Furuta F, Noda M, Suzuki K Control of lymphocyte egress from lymph nodes through beta2-adrenergic receptors. J Exp Med, 2014-11-24;211(13):2583-98. 2014-11-24 [PMID: 25422496] (Flow Cytometry, Mouse)
• Nomachi , Akira, Yoshinaga , Masanori, Liu , Jaron, Kanchanawong , Pakorn, Tohyama , Kiyoshi, Thumkeo , Dean, Watanabe , Takeshi, Narumiya , Shuh, Hirata , Takako Moesin controls clathrin-mediated S1PR1 internalization in T cells. PLoS ONE, 2013-12-16;8(12):e82590. 2013-12-16 [PMID: 24358210] (Flow Cytometry, Mouse)
• Arnon , Tal I, Horton , Robert M, Grigorova , Irina L, Cyster , Jason G Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress. Nature, 2012-12-23;493(7434):684-8. 2012-12-23 [PMID: 23263181] (Flow Cytometry, Mouse)
• Green JA, Suzuki K, Cho B, Willison LD, Palmer D, Allen CD, Schmidt TH, Xu Y, Proia RL, Coughlin SR, Cyster JG The sphingosine 1-phosphate receptor S1P(2) maintains the homeostasis of germinal center B cells and promotes niche confinement. Nat. Immunol., 2011-06-05;12(7):672-80. 2011-06-05 [PMID: 21642988] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: S1PR1
• Uniprot:
  • O08530()