CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free

Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Staining of CD66b on FFPE section of human liver. Secondary Antibody is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. Cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific wi

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Human peripheral blood was stained with Mouse IgM isotype control (left panel), Mouse anti-Human CEACAM-8/CD66b clone G10F5 (right panel), followed by APC-conjugated Goat anti-Mouse IgM secondary antibody. Image from the standard format of this antibody.

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Image from verified customer review.

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Analysis using the Alexa Fluor (R) 700 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with Alexa Fluor 700. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C.

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Analysis using the DyLight 405 conjugate of NB100-77808. Staining of CD66b in human PBMCs using anti-CD66b antibody. Image from verified customer review.

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Analysis using the FITC conjugate of NB100-77808. Staining of Human peripheral whole blood granulocytes G10F5 FITC.

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Analysis using the PE conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with PE. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Image from verified customer review.

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free [NBP2-80664] - Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC Image from the standard format of this antibody.

• Reactivity: Hu, Mu
• Applications: Flow, IHC, CyTOF-ready
• Clone: G10F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free Summary

• Immunogen: Human peripheral blood cells.
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEACAM8
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1ug/million cells
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: It is recommended that CD66b clone G10F5 be titrated for optimal performance for each application. This antibody is CyTOF ready.

Reactivity Notes

Predicted to cross react with Chimpanzee.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: IgM purified

Alternate Names for CEACAM8/CD66b Antibody (G10F5) - Azide and BSA Free

• Carcinoembryonic antigen CGM6
• carcinoembryonic antigen gene family member 6
• carcinoembryonic antigen-related cell adhesion molecule 8
• CD66b antigen
• CD66b
• CD67 antigen
• CD67
• CEACAM8
• CEACAM-8
• CGM6
• NCA-95
• Non-specific cross-reacting antigen NCA-95

Background

CEACAM-8/CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CEACAM-8/CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CEACAM-8/CD66b expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c. Clone G10F5 can be used for a number of applications including Flow Cytometry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEACAM8/CD66b Antibody (NBP2-80664)(1)

Publication using NBP2-80664

• Ak Ç, Sayar Z, Thibault G, Burlingame EA et Al. Multiplex imaging of localized prostate tumors reveals altered spatial organization of AR-positive cells in the microenvironment iScience 2024-09-09 [PMID: 39246442]

Bioinformatics

• Gene Symbol: CEACAM8