Flow Cytometry is a laser based biological assay used for cell detection, counting and sorting of specific biomarkers. Cells are first suspended in solution and fluorescently labeled antibodies are applied. Then the cells are passed single-file through a series of lasers which analyze size, shape and the fluorescent characteristics of each cell. Results can then be electronically gated to filter the data to a specific cell type and whether or not they contain bound antibodies. This form of assay is particularly useful for analyzing blood samples.
Novus offers a range of support options for flow cytometry when you buy a product to help you with your flow cytometry experiment including over 1,000 Flow images using our pre-conjugated antibodies:
![Immunocytochemistry/Immunofluorescence: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - The stem cell marker (Nanog) staining of iPS-BP cells. Nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP1-42823-img0014.jpg "Immunocytochemistry/Immunofluorescence: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - The stem cell marker (Nanog) staining of iPS-BP cells. Nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license.")
![Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY license. OCT4 Antibody [NB100-2379]](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-NBP1-42823-img0016.jpg)
![Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Western-Blot-NBP1-42823-img0013.jpg)
![Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Nanog-inducible mouse model. Representative hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) for NANOG, Ki67 and LORICRIN of TPA-treated CTR and TG mice (bars correspond to 50 um). Statistical significance was determined by the two-tailed Student's t test: (*) p < 0.05; (**) p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep10205), licensed under a CC-BY license. Nanog Antibody [NB100-58842]](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunohistochemistry-NBP1-42823-img0015.jpg)
![Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Western-Blot-NBP1-42823-img0011.jpg)
![Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - SOX2 staining (NB110-37235) of human uterus, endometrial glands.](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunohistochemistry-NBP1-42823-img0017.jpg)
![Live Imaging Microscopy: Human ESC Marker Antibody Pack [NBP1-42825] - Pluripotent Li-iPSCs colonies derived from reprogramming of PLCs. iPSC colonies (encircled by dashed line) were positive for the surface pluripotency marker TRA-1-60 (red) during live staining. TRA-1-60 staining (red) is shown before (C) and afteroverlaying onto the corresponding phase contrast image (D). Scale bars = 250um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0221762) licensed under a CC-BY license. TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730]](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Live-Imaging--Microscopy-NBP1-42825-img0011.jpg "Live Imaging Microscopy: Human ESC Marker Antibody Pack [NBP1-42825] - Pluripotent Li-iPSCs colonies derived from reprogramming of PLCs. iPSC colonies (encircled by dashed line) were positive for the surface pluripotency marker TRA-1-60 (red) during live staining. TRA-1-60 staining (red) is shown before (C) and afteroverlaying onto the corresponding phase contrast image (D). Scale bars = 250um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0221762) licensed under a CC-BY license. TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730]")
![Immunocytochemistry/Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825] - TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # NB100-1833) at 10 ug/mL for 3 hours at room temperature. Cells were stained using an Anti-Mouse IgM Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. TRA-1-81 Antibody (TRA-1-81) [NB100-1833]](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP1-42825-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825] - Analysis of human embryonic stem cells stained with SSEA-3 antibody detected with Alexa Fluor 488 anti-rat IgM secondary antibody. SSEA-3 Antibody (MC-631) [NB100-1832]](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP1-42825-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Human ESC Marker Antibody Pack [NBP1-42825] - Detection of Mouse Nanog on HeLa whole cell lysate using NB100-588. Nanog was also IPed using NB100-587 and another Nanog. IP reactions were controlled using normal rabbit IgG. IPed Nanog was blotted using NB100-587.](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Immunoprecipitation-NBP1-42825-img0009.jpg)
![Western Blot: Human ESC Marker Antibody Pack [NBP1-42825] - Whole cell protein from human ES cells (lane 1), human NTERA-2 cells (lane 2), mouse embryonic fibroblasts (MEF, lane 3) and mouse V6.5 ES cells (lane 4, NBP1-97433) were separated on a 7.5% gel and transferred to PVDF membrane. The membrane was then incubated with anti-TRA1-60(R) antibody at 2 ug/ml and detected with an anti-mouse IgM secondary antibody using chemiluminescence. Note how this antibody specifically detects the TRA1-60(R) epitope only on human cells. TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730]](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Western-Blot-NBP1-42825-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825] - SSEA1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red). SSEA-1 Antibody (MC-480) [NB100-1831]](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP1-42825-img0014.jpg)
![Flow Cytometry: Human ESC Marker Antibody Pack [NBP1-42825] - FACS analysis of Nanog expression in primary mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Flow-Cytometry-NBP1-42825-img0015.jpg)
![Western Blot: Human ESC Marker Antibody Pack [NBP1-42825] - Detection of OCT4 in mouse brain lysate](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Western-Blot-NBP1-42825-img0005.jpg)
![Flow Cytometry: Human ESC Marker Antibody Pack [NBP1-42825] - FACS staining of NTERA-2 cells using NB100-1831 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Flow-Cytometry-NBP1-42825-img0003.jpg)
![Flow Cytometry: Human ESC Marker Antibody Pack [NBP1-42825] - FACS staining of NTERA-2 cells using NB100-2379 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Flow-Cytometry-NBP1-42825-img0004.jpg)
![Functional: Autophagy/Cytotoxicity Dual Staining Other Kit [KA1299] - Tamoxifen increases autophagy but not cell death in HepG2 cells as measured by fluorescence microscopy. Panel A : MDC staining of HepG2 cells treated with vehicle. There is a basal level of autophagy, indicated by faint silver dot staining of autophagic vacuoles. Panel B : Propidium iodide staining of HepG2 cells treated with vehicle. There are few dead cells with only background staining of propidium iodide. Panel C : MDC staining of HepG2 cells treated with 10 uM Tamoxifen. Panel D : Propidium iodide staining of HepG2 cells treated with 10 uM Tamoxifen, showing a similar staining pattern to that of cells treated with vehicle](http://images.novusbio.com/fullsize/Autophagy-Cytotoxicity-Dual-Staining-Other-Kit-Functional-KA1299-img0003.jpg "Functional: Autophagy/Cytotoxicity Dual Staining Other Kit [KA1299] - Tamoxifen increases autophagy but not cell death in HepG2 cells as measured by fluorescence microscopy. Panel A : MDC staining of HepG2 cells treated with vehicle. There is a basal level of autophagy, indicated by faint silver dot staining of autophagic vacuoles. Panel B : Propidium iodide staining of HepG2 cells treated with vehicle. There are few dead cells with only background staining of propidium iodide. Panel C : MDC staining of HepG2 cells treated with 10 uM Tamoxifen. Panel D : Propidium iodide staining of HepG2 cells treated with 10 uM Tamoxifen, showing a similar staining pattern to that of cells treated with vehicle")
![Anti-Mouse Ig (H+L) Comp-Bead 3 Population (5.5 um) Kit [NBP3-00497] - Anti-Mouse Ig (H+L) Comp-Bead 3 Population (5.5 um) [NBP3-00497] - Dot plots of NBP3-00497 exposed to mouse monoclonal fluorescent conjugate.](http://images.novusbio.com/fullsize/Anti-Mouse-Ig-H+L-Comp-Bead-3-Population-5-5-um-NBP3-00497-img0001.jpg "Anti-Mouse Ig (H+L) Comp-Bead 3 Population (5.5 um) Kit [NBP3-00497] - Anti-Mouse Ig (H+L) Comp-Bead 3 Population (5.5 um) [NBP3-00497] - Dot plots of NBP3-00497 exposed to mouse monoclonal fluorescent conjugate.")
![Amine Reactive Comp-Bead 2 Population [NBP3-00496] - Histogram. Amine Reactive Comp-Beads stained with Biolegend Zombie Green(Tm).](http://images.novusbio.com/fullsize/Amine-Reactive-Comp-Bead-2-Population-NBP3-00496-img0001.jpg "Amine Reactive Comp-Bead 2 Population [NBP3-00496] - Histogram. Amine Reactive Comp-Beads stained with Biolegend Zombie Green(Tm).")
![Flow Cytometry: Amine Reactive Comp-Bead 2 Population Kit [NBP3-00496] - Beads used for flow cytometry compensation calculations. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp3-00496_amine-reactive-comp-bead-2-population-kit-flow-cytometry-1892024144937..png)
![Flow Cytometry: Fluorescent Particle Array (10 peaks) Kit [NBP3-18584] - Dot Plots for NBP3-18584 on a BD Fortessa X20](http://images.novusbio.com/fullsize/Fluorescent-Particle-Array-10-peaks-Kit-Flow-Cytometry-NBP3-18584-img0001.jpg "Flow Cytometry: Fluorescent Particle Array (10 peaks) Kit [NBP3-18584] - Dot Plots for NBP3-18584 on a BD Fortessa X20")
![Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um) Kit [NBP3-00499] - Histogram of flow cytometry data showing fron and side scatter and the three populations in the FITC channel. Dot plots show uncompensated and compensation populations in the FITC Log X PE Log.](http://images.novusbio.com/fullsize/Anti-Mouse-Ig-H+L-Comp-Bead-3-Population-7-5-um-Kit-NBP3-00499-img0001.jpg "Anti-Mouse Ig (H+L) Comp-Bead 3 Population (7.5 um) Kit [NBP3-00499] - Histogram of flow cytometry data showing fron and side scatter and the three populations in the FITC channel. Dot plots show uncompensated and compensation populations in the FITC Log X PE Log.")
![Anti-Mouse Ig (H+L) Comp-Bead 2 Population (3.0-3.4 um) Kit [NBP3-11302] - Histograms of NBP3-11302 exposed to to mouse, rat and hamster antibodies.](http://images.novusbio.com/fullsize/Anti-Mouse-Ig-H+L-Comp-Bead-2-Population-3-0-3-4-um-Kit-NBP3-11302-img0001.jpg "Anti-Mouse Ig (H+L) Comp-Bead 2 Population (3.0-3.4 um) Kit [NBP3-11302] - Histograms of NBP3-11302 exposed to to mouse, rat and hamster antibodies.")