VE-Cadherin Antibody (123413) [Allophycocyanin]

HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VE-Cadherin APC-conjugated Monoclonal Antibody (Catalog # FAB9381A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). Cells were stained in a buffer containing Ca2+and Mg2+. View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 123413
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

VE-Cadherin Antibody (123413) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
  Asp48-Gln593
  Accession # P33151
• Specificity: Detects human VE-Cadherin in Western blots. In Western blots, 25% cross-reactivity with recombinant mouse VE‑Cadherin and no cross‑reactivity with recombinant human (rh) Cadherin-17 or rhP-Cadherin is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VE-Cadherin Antibody (123413) [Allophycocyanin]

• 7B4 antigen
• 7B4
• cadherin 5, type 2 (vascular endothelium)
• cadherin 5, type 2, VE-cadherin (vascular epithelium)
• Cadherin-5
• CD144 antigen
• CD144
• CDH5
• endothelial-specific cadherin
• FLJ17376
• Vascular endothelial cadherin
• VECadherin
• VE-Cadherin

Background

Vascular Endothelial (VE)-Cadherin (VE-CAD), also called 7B4 and Cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha , beta , and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-Cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-Cadherin proteins share approximately 74% homology.

1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
3. Overduin, M. et al. (1995) Science 267:386.
4. Takeichi, M. (1991) Science 251:1451.
5. Nose, A. et al. (1987) EMBO J. 6:3655.
6. Carmeliet, P. et al. (1999) Cell 98:147.
7. Gory-Faure, S. et al. (1999) Development 126:2093.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VE-Cadherin Antibody (FAB9381A)(1)

Publication using FAB9381A

• B Bassetti, C Carbucicch, V Catto, E Gambini, E Rurali, A Bestetti, G Gaipa, D Belotti, F Celeste, M Parma, S Righetti, L Biava, M Arosio, A Bonomi, P Agostoni, P Scacciatel, F Achilli, G Pompilio Linking cell function with perfusion: insights from the transcatheter delivery of bone marrow-derived CD133+ cells in ischemic refractory cardiomyopathy trial (RECARDIO) Stem Cell Res Ther, 2018-09-14;9(1):235. 2018-09-14 [PMID: 30217223] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CDH5
• Uniprot:
  • P33151()