Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1983-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-167SS | |||
| Azide and BSA Free | NBP2-80826 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1118-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-442SS | |||
| Azide and BSA Free | NBP2-80727 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-644SS | |||
| Azide and BSA Free | NBP2-80970 | |||
![Western Blot: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Western Blot analysis of Rat brain membrane lysate showing detection of CaM Kinase II alpha protein using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:1000.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Western-Blot-NB100-1983-img0015.jpg "Western Blot: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Western Blot analysis of Rat brain membrane lysate showing detection of CaM Kinase II alpha protein using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear Staining.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunocytochemistry-Immunofluorescence-NB100-1983-img0018.jpg)
![Immunohistochemistry: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Immunohistochemistry analysis using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunohistochemistry-NB100-1983-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunocytochemistry-Immunofluorescence-NB100-1983-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: dissociated hippocampal neurons. Species: Mouse. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunocytochemistry-Immunofluorescence-NB100-1983-img0014.jpg)
![Immunohistochemistry: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Immunohistochemistry analysis using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunohistochemistry-NB100-1983-img0016.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) Alexa Fluor® 647.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0008.jpg "Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) Alexa Fluor® 647.")
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of NALM-6 human pre-B cell leukemia cell line Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0001.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD9 (MEM-61) Dy161.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0002.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0003.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) APC-CyTM7.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0004.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0005.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) APC.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0006.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) PE.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0007.jpg)
![CyTOF-ready: CD9 Antibody (MEM-61) [NB500-327] - Analysis of CD9 antibody on human tissue. Scale, 50um. Image from verified customer review.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-CyTOF-ready-NB500-327-img0009.jpg)
![N/A IL-27 p28/IL-30 [HRP]](http://images.novusbio.com/fullsize2/DATA_IL27_p28_M2728_ELISA_930.jpg "N/A IL-27 p28/IL-30 [HRP]")
![N/A IL-27 p28/IL-30 [HRP]](http://images.novusbio.com/fullsize2/IL-27_p28_M2728_ELISA_233.jpg)
![N/A CXCL4/PF4 [HRP]](http://images.novusbio.com/fullsize2/DATA_CXCL4_DPF40_ELISA_787.jpg "N/A CXCL4/PF4 [HRP]")
![N/A CXCL4/PF4 [HRP]](http://images.novusbio.com/fullsize2/CXCL4_DPF40_ELISA_473.jpg)
![Western Blot: LANA2 Antibody (CM-A807) [NB200-167] - Detection of LANA2 in infected lysates (30 ug) using 2 ug/ml of NB 200-167. ECL detection 1.5 minutes. Lane 1: BCBL-1 (KSHV+) Lane 2: BC-1 (KSHV+/EBV+) Lane 3: BCP-1 (KSHV+)](http://images.novusbio.com/fullsize/LANA2-Antibody-CM-A807-Western-Blot-NB200-167-img0003.jpg "Western Blot: LANA2 Antibody (CM-A807) [NB200-167] - Detection of LANA2 in infected lysates (30 ug) using 2 ug/ml of NB 200-167. ECL detection 1.5 minutes. Lane 1: BCBL-1 (KSHV+) Lane 2: BC-1 (KSHV+/EBV+) Lane 3: BCP-1 (KSHV+)")
![Immunohistochemistry-Paraffin: LANA2 Antibody (CM-A807) [NB200-167] - vIRF3 expression in KS tissues. We obtained KS tissue microarrays from AIDS Cancer Specimen Resource and performed immunohistochemistry, visualized by Aperio F.L. digital pathological scanning. A biopsy sample of KS TMA was stained with anti-LANA. Representative image shows the LANA staining of skin KS lesion. Image collected and cropped by CiteAb from the following publication (//mbio.asm.org/lookup/doi/10.1128/mBio.02217-17), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LANA2-Antibody-CM-A807-Immunohistochemistry-Paraffin-NB200-167-img0004.jpg)
![Immunohistochemistry: LANA2 Antibody (CM-A807) - BSA Free [NB200-167] - vIRF3 expression in KS tissues. We obtained KS tissue microarrays from AIDS Cancer Specimen Resource & performed immunohistochemistry, visualized by Aperio F.L. digital pathological scanning. A biopsy sample of KS TMA was stained with anti-LANA & anti-vIRF3 (CM-A807). Representative images show the LANA staining of skin KS lesion & the vIRF3 staining of skin, tonsil, & mouth KS lesions & positive staining corresponding to either LANA or vIRF3 in total KS biopsy specimens embedded in TMA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29339432), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-167_mouse-monoclonal-lana2-antibody-cm-a807-310202415371954.jpg)
![N/A IL-18 BPa/IL18BP [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL18_BPa_DY119_ELISA_1598.jpg "N/A IL-18 BPa/IL18BP [Biotin]")
![Flow Cytometry: Ethenoadenosine Antibody (1G4) [NB600-442] - Chicken erythrocytes (1% v/v), isolated by density gradient centrifugation, were transiently transfected with a Flag-tagged chicken ART4 containing plasmid or the empty plasmid (pSecTag B), and incubated at 37C in the presence or absence of 200 uM etheno-NAD+ for 30 min. After washing, cells were stained with an etheno-adenosine specific antibody (1G4) or an anti-Flag antibody and the respective isotype control stained with the 1G4 antibody. Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-86), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ethenoadenosine-Antibody-1G4-Flow-Cytometry-NB600-442-img0002.jpg "Flow Cytometry: Ethenoadenosine Antibody (1G4) [NB600-442] - Chicken erythrocytes (1% v/v), isolated by density gradient centrifugation, were transiently transfected with a Flag-tagged chicken ART4 containing plasmid or the empty plasmid (pSecTag B), and incubated at 37C in the presence or absence of 200 uM etheno-NAD+ for 30 min. After washing, cells were stained with an etheno-adenosine specific antibody (1G4) or an anti-Flag antibody and the respective isotype control stained with the 1G4 antibody. Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-86), licensed under a CC-BY license.")
![Flow Cytometry: Ethenoadenosine Antibody (1G4) [NB600-442] - Detection of ADP-ribosylated proteins by flow cytometry. C-33A cells (1 x 10^7/mL), transiently transfected with a Flag-tagged chicken ART4 containing plasmid, were incubated at 37C in the presence or absence of 200 uM etheno-NAD+ for 30 min. After washing, cells were stained with an etheno-adenosine specific antibody (1G4). Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-86), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ethenoadenosine-Antibody-1G4-Flow-Cytometry-NB600-442-img0001.jpg)
![Flow Cytometry: Ethenoadenosine Antibody (1G4) [NB600-442] - Etheno-ADP-ribosylation of cell-surface proteins on C3H-10T1/2 cells. C3H-10T 1/2 cells at different stages of differentiation (progenitors, MyoD-myoblasts, myotubes) were incubated for 30 minutes in the absence (dashed lines) or presence (solid lines) of 100 uM etheno-NAD. Cells were then washed, incubated with the primary monoclonal antibody 1G4, stained with a FITC-conjugated goat-anti-mouse secondary antibody and subjected to FACS analysis. Data show one representative experiments out of three. Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-91), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ethenoadenosine-Antibody-1G4-Flow-Cytometry-NB600-442-img0003.jpg)
![Immunohistochemistry: Survivin Antibody (8E2) [NB500-644] - Immunohistochemical analysis of Survivin in human breast cancer tissue using Survivin Antibody (8E2) [NB500-644] and DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Immunohistochemistry-NB500-644-img0005.jpg "Immunohistochemistry: Survivin Antibody (8E2) [NB500-644] - Immunohistochemical analysis of Survivin in human breast cancer tissue using Survivin Antibody (8E2) [NB500-644] and DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: Survivin Antibody (8E2) [NB500-644] - Immunocytochemical analysis using Survivin Antibody (8E2) [NB500-644] was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Immunocytochemistry-Immunofluorescence-NB500-644-img0004.jpg)
![Flow (Intracellular): Survivin Antibody (8E2) [NB500-644] - An intracellular stain was performed on Daudi cells with Survivin Antibody (8E2) [NB500-644] (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Flow-Intracellular-NB500-644-img0006.jpg)
![N/A S100A8/S100A9 Heterodimer [Biotin]](http://images.novusbio.com/fullsize2/DATA_S100A8_S100A9_Heterodimer_DY822605_ELISA_2535.jpg "N/A S100A8/S100A9 Heterodimer [Biotin]")
![N/A IL-10 [HRP]](http://images.novusbio.com/fullsize2/DATA_IL10_P1000_ELISA_1053.jpg "N/A IL-10 [HRP]")
![N/A IL-10 [HRP]](http://images.novusbio.com/fullsize2/IL-10_P1000_ELISA_339.jpg)
![N/A Progranulin/PGRN [HRP]](http://images.novusbio.com/fullsize2/DATA_Progranulin_DPGRN0_ELISA_790.jpg "N/A Progranulin/PGRN [HRP]")
![N/A Progranulin/PGRN [HRP]](http://images.novusbio.com/fullsize2/DATA_Progranulin_DPGRN0_ELISA_791.jpg)
![N/A Progranulin/PGRN [HRP]](http://images.novusbio.com/fullsize2/Progranulin_DPGRN0_ELISA_159.jpg)