Mouse IL-27 p28/IL-30 Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse IL-27 p28/IL-30 Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse IL-27 p28 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-27 p28 in cell culture supernates, mouse serum, and plasma. It contains NS0-expressed recombinant mouse IL-27 p28 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-2...7 p28 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IL-27 p28.
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• Specificity: Natural and recombinant mouse IL-27 p28
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Il27

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-27 p28/IL-30 Quantikine ELISA Kit

• IL27 p28
• IL-27 p28
• IL27;IL-27;IL27A;IL-27A;IL27p28;IL-27p28;IL30;p28
• IL-30

Background

IL-27 is a heterodimeric cytokine that is composed of EBI3 (which is related to the p40 subunit of IL-12 and IL-23) and p28 (which is related to the p35 chain of IL-12). IL-27 is expressed by monocytes, endothelial cells, and dendritic cells, and it signals through a receptor complex composed of IL-27 R alpha/WSX-1/TCCR and gp130. IL-27 induces IL-12 receptor expression on naïve CD4+ T cells, sensitizing them to subsequent IL-12 induced Th1 development. It also functions as an anti-inflammatory cytokine by limiting T and NKT cell activity.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-27 p28/IL-30 (M2728)(21)

Publications using M2728

• Zhu, T;Shen, Q;Shen, L;Wang, Y;Zhu, B;Ma, L;Feng, S;Wang, C;Yan, S;Li, J;Chen, Z;Zhou, J;Huang, H;Li, B;Shen, Z;Wang, Q;Wang, J;Gwinner, W;Scheffner, I;Rong, S;Yang, B;Wang, J;Haller, H;Han, X;Guo, G;Yin, Z;Jin, J;Lan, HY;Chen, J;Jiang, H; Senescence-induced p21high macrophages contributed to CD8+ T cells-related immune hyporesponsiveness in kidney transplantation via Zfp36/IL-27 axis Cell discovery 2025-04-15 [PMID: 40234384] (Mouse)
• Aldridge, DL;Lanzar, Z;Phan, AT;Christian, DA;Pardy, R;Min, B;Kedl, RM;Hunter, CA; IL-27 limits HSPC differentiation during infection and protects from stem cell exhaustion bioRxiv : the preprint server for biology 2025-01-19 [PMID: 39868131] (Mouse)
• Albarracin, L;Dentice Maidana, S;Fukuyama, K;Elean, M;Argañaraz Aybar, JN;Suda, Y;Nishiyama, K;Kitazawa, H;Villena, J; Orally Administered Lactobacilli Strains Modulate Alveolar Macrophages and Improve Protection Against Respiratory Superinfection Biomolecules 2024-12-14 [PMID: 39766307] (Mouse)
• Murphy, B;Miyamoto, T;Manning, BS;Mirji, G;Ugolini, A;Kannan, T;Hamada, K;Zhu, YP;Claiborne, DT;Huang, L;Zhang, R;Nefedova, Y;Kossenkov, A;Veglia, F;Shinde, R;Zhang, N; Intraperitoneal activation of myeloid cells clears ascites and reveals IL27-dependent regression of metastatic ovarian cancer bioRxiv : the preprint server for biology 2024-06-29 [PMID: 38979222] (Mouse)
• Zhou, B;Elean, M;Arce, L;Fukuyama, K;Tomotsune, K;Dentice Maidana, S;Saha, S;Namai, F;Nishiyama, K;Vizoso-Pinto, MG;Villena, J;Kitazawa, H; The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract Microorganisms 2024-06-16 [PMID: 38930591] (Mouse)
• Elean, M;Raya Tonetti, F;Fukuyama, K;Arellano-Arriagada, L;Namai, F;Suda, Y;Gobbato, N;Nishiyama, K;Villena, J;Kitazawa, H; Immunobiotic Ligilactobacillus salivarius FFIG58 Confers Long-Term Protection against Streptococcus pneumoniae International journal of molecular sciences 2023-10-30 [PMID: 37958756] (Mouse)
• M Tomokiyo, FR Tonetti, H Yamamuro, R Shibata, K Fukuyama, N Gobbato, L Albarracin, MSR Rajoka, AKMH Kober, W Ikeda-Ohts, J Villena, H Kitazawa Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity Cells, 2022-09-25;11(19):. 2022-09-25 [PMID: 36230948] (Mouse)
• B Min, D Kim, MJ Feige IL-30� (IL-27A): a familiar stranger in immunity, inflammation, and cancer Experimental & Molecular Medicine, 2021-05-28;53(5):823-834. 2021-05-28 [PMID: 34045653] (Mouse)
• Sha X, Meng S, Li X, Xi H, Maddaloni M, Pascual D, Shan H, Jiang X, Wang H, Yang X Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway. J Biol Chem, 2015-06-17;290(31):19307-18. 2015-06-17 [PMID: 26085094] (Mouse)
• de Almeida Nagata D, Demoor T, Ptaschinski C, Ting H, Jang S, Reed M, Mukherjee S, Lukacs N IL-27R-mediated regulation of IL-17 controls the development of respiratory syncytial virus-associated pathogenesis. Am J Pathol, 2014-04-13;184(6):1807-18. 2014-04-13 [PMID: 24726498] (Mouse)
• Owens BM, Beattie L, Moore JW, Brown N, Mann JL, Dalton JE, Maroof A, Kaye PM IL-10-Producing Th1 Cells and Disease Progression Are Regulated by Distinct CD11c+ Cell Populations during Visceral Leishmaniasis. PLoS Pathog., 2012-07-26;8(7):e1002827. 2012-07-26 [PMID: 22911108] (Mouse)
• Stumhofer JS, Tait ED, Quinn WJ, Hosken N, Spudy B, Goenka R, Fielding CA, O'Hara AC, Chen Y, Jones ML, Saris CJ, Rose-John S, Cua DJ, Jones SA, Elloso MM, Grotzinger J, Cancro MP, Levin SD, Hunter CA A role for IL-27p28 as an antagonist of gp130-mediated signaling. Nat. Immunol., 2010-11-07;11(12):1119-26. 2010-11-07 [PMID: 21057510] (Mouse)
• Molle C, Goldman M, Goriely S Critical role of the IFN-stimulated gene factor 3 complex in TLR-mediated IL-27p28 gene expression revealing a two-step activation process. J. Immunol., 2010-01-18;184(4):1784-92. 2010-01-18 [PMID: 20083668] (Mouse)
• Vultaggio A, Nencini F, Fitch PM, Fili L, Maggi L, Fanti P, deVries A, Beccastrini E, Palandri F, Manuelli C, Bani D, Giudizi MG, Guarna A, Annunziato F, Romagnani S, Maggi E, Howie SE, Parronchi P Modified adenine (9-benzyl-2-butoxy-8-hydroxyadenine) redirects Th2-mediated murine lung inflammation by triggering TLR7. J. Immunol., 2009-01-15;182(2):880-9. 2009-01-15 [PMID: 19124731] (Mouse)
• Shinohara ML, Kim JH, Garcia VA, Cantor H Engagement of the type I interferon receptor on dendritic cells inhibits T helper 17 cell development: role of intracellular osteopontin. Immunity, 2008-07-18;29(1):68-78. 2008-07-18 [PMID: 18619869] (Mouse)
• Guo B, Chang EY, Cheng G The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice. J. Clin. Invest., 2008-05-01;118(5):1680-90. 2008-05-01 [PMID: 18382764] (Mouse)
• Xu J, Racke MK, Drew PD Peroxisome proliferator-activated receptor-alpha agonist fenofibrate regulates IL-12 family cytokine expression in the CNS: relevance to multiple sclerosis. J. Neurochem., 2007-12-01;103(5):1801-10. 2007-12-01 [PMID: 17727629] (Mouse)
• Fitzgerald DC, Ciric B, Touil T, Harle H, Grammatikopolou J, Das Sarma J, Gran B, Zhang GX, Rostami A Suppressive effect of IL-27 on encephalitogenic Th17 cells and the effector phase of experimental autoimmune encephalomyelitis. J. Immunol., 2007-09-01;179(5):3268-75. 2007-09-01 [PMID: 17709543] (Mouse)
• Sheibanie AF, Yen JH, Khayrullina T, Emig F, Zhang M, Tuma R, Ganea D The proinflammatory effect of prostaglandin E2 in experimental inflammatory bowel disease is mediated through the IL-23-->IL-17 axis. J. Immunol., 2007-06-15;178(12):8138-47. 2007-06-15 [PMID: 17548652] (Mouse)
• Molle C, Nguyen M, Flamand V, Renneson J, Trottein F, De Wit D, Willems F, Goldman M, Goriely S IL-27 synthesis induced by TLR ligation critically depends on IFN regulatory factor 3. J. Immunol., 2007-06-15;178(12):7607-15. 2007-06-15 [PMID: 17548596] (Mouse)
• Liu J, Guan X, Ma X Regulation of IL-27 p28 gene expression in macrophages through MyD88- and interferon-gamma-mediated pathways. J. Exp. Med., 2007-01-16;204(1):141-52. 2007-01-16 [PMID: 17227910] (Mouse)

Bioinformatics

• Gene Symbol: Il27