Ethenoadenosine Antibody (1G4) [CoraFluor™ 1]

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Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IP, CyTOF-ready
Clone
1G4
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

Ethenoadenosine Antibody (1G4) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Ethenoadenosine
Specificity
This is specific for ethenoadenosine and ethenodadenosine.
Isotype
IgG2 Lambda
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Ethenoadenosine Antibody (1G4) [CoraFluor™ 1]

  • C12H13N5O4
  • N6-Ethenoadenosine

Background

Ethenobases are adduct products of vinyl chloride, an occupational carcinogen. The sensitivity and specificity of the ethenoadenosine antibody have been determined by enzyme-linked immunosorbent assay. It reacts with both the ribose and deoxyribose form of the adduct. In addition, previous lots of this antibody have been shown to detect etheno-ADP-ribosylation of cell surface proteins by flow cytometry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ethenoadenosine Antibody (NB600-442CL1) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Ethenoadenosine Products

Array NB600-442CL1

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