Ethenoadenosine Antibody (1G4) [FITC]

• Reactivity: All
• Applications: WB, ELISA, Flow, ICC/IF, IP, CyTOF-ready
• Clone: 1G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

Ethenoadenosine Antibody (1G4) [FITC] Summary

• Immunogen: Ethenoadenosine
• Localization: Nuclear
• Specificity: This is specific for ethenoadenosine and ethenodadenosine.
• Isotype: IgG2 Lambda
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Ethenoadenosine Antibody (1G4) [FITC]

• C12H13N5O4
• N6-Ethenoadenosine

Background

ADP-ribosylation represents a posttranslational modification that involves transfer of ADP-ribose moiety from NAD+ to a specific amino acid in target protein (greatly modifying protein function) with the release of nicotinamide moiety. It was originally discovered as a mechanism by which potent bacterial toxins, such as Diphtheria toxin and Cholera toxin, modify and inactivate human target proteins, and these toxins were suggested to mimic mammalian endogenous ADP-ribosyltransferases. ART2 is an ecto-ADP-ribosyltransferases family (ART1-ART5) member which distantly resembles ADP-ribosylating bacterial toxins. It is expressed by mature T cells in which it ADP-ribosylates the integrin LFA-1 as well as other cell surface proteins, and this ART2-mediated ADP-ribosylation of T cell surface proteins provides a novel means for triggering T cell apoptosis. Ethenoadenosine moiety is contained in NAD analogue etheno-NAD which serves as an efficient substrate for ADP-ribosyltransferases and ethenoadenosine specific antibody clone 1G4 can be used for detection of ADP-ribosylated amino acids (ADP-ribosylation of cellular proteins) by FACS/FLOW, ICC-IF and Western blot applications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.