Ethenoadenosine Antibody (1G4) [FITC]

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Product Details

Summary
Reactivity AllSpecies Glossary
Applications ELISA, Flow, ICC/IF
Clone
1G4
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Ethenoadenosine Antibody (1G4) [FITC] Summary

Immunogen
Ethenoadenosine
Localization
Nuclear
Specificity
This is specific for ethenoadenosine and ethenodadenosine.
Isotype
IgG2 Lambda
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
This Ethenoadenosine antibody is useful for Flow Cytometry (PMID 9774627), Immunocytochemistry/Immunofluorescence, Immunoprecipitation, ELISA and Western blot.

Reactivity Notes

All species.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Background

ADP-ribosylation represents a posttranslational modification that involves transfer of ADP-ribose moiety from NAD+ to a specific amino acid in target protein (greatly modifying protein function) with the release of nicotinamide moiety. It was originally discovered as a mechanism by which potent bacterial toxins, such as Diphtheria toxin and Cholera toxin, modify and inactivate human target proteins, and these toxins were suggested to mimic mammalian endogenous ADP-ribosyltransferases. ART2 is an ecto-ADP-ribosyltransferases family (ART1-ART5) member which distantly resembles ADP-ribosylating bacterial toxins. It is expressed by mature T cells in which it ADP-ribosylates the integrin LFA-1 as well as other cell surface proteins, and this ART2-mediated ADP-ribosylation of T cell surface proteins provides a novel means for triggering T cell apoptosis. Ethenoadenosine moiety is contained in NAD analogue etheno-NAD which serves as an efficient substrate for ADP-ribosyltransferases and ethenoadenosine specific antibody clone 1G4 can be used for detection of ADP-ribosylated amino acids (ADP-ribosylation of cellular proteins) by FACS/FLOW, ICC-IF and Western blot applications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB600-442APC
Biotin Labeled NB600-442B
DyLight 350 Labeled NB600-442UV
DyLight 405 Labeled NB600-442V
DyLight 488 Labeled NB600-442G
DyLight 550 Labeled NB600-442R
DyLight 650 Labeled NB600-442C
DyLight 680 Labeled NB600-442FR
DyLight 755 Labeled NB600-442IR
HRP Labeled NB600-442H
PE Labeled NB600-442PE
PerCP Labeled NB600-442PCP

Additional Ethenoadenosine Products

Ethenoadenosine NB600-442F

Research Areas for Ethenoadenosine Antibody (NB600-442F)

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