Ethenoadenosine Antibody (1G4) - Azide and BSA Free

Flow Cytometry: Ethenoadenosine Antibody (1G4) - Azide and BSA Free [NBP2-80727] - Detection of ADP-ribosylated proteins by flow cytometry. C-33A cells (1 × 107/ml), transiently transfected with a Flag-tagged chicken ART4 containing plasmid, were incubated at 37C in the presence or absence of 200 uM etheno-NAD+ for 30 min. After washing, cells were stained with an etheno-adenosine specific antibody (1G4). Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-86), licensed under a CC-BY license. Image from the standard format of this antibody.

Flow Cytometry: Ethenoadenosine Antibody (1G4) - Azide and BSA Free [NBP2-80727] - Chicken erythrocytes (1% v/v), isolated by density gradient centrifugation, were transiently transfected with a Flag-tagged chicken ART4 containing plasmid or the empty plasmid (pSecTag B), and incubated at 37C in the presence or absence of 200 uM etheno-NAD+ for 30 min. After washing, cells were stained with an etheno-adenosine specific antibody (1G4) or an anti-Flag antibody and the respective isotype control stained with the 1G4 antibody. Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-86), licensed under a CC-BY license. Image from the standard format of this antibody.

Flow Cytometry: Ethenoadenosine Antibody (1G4) - Azide and BSA Free [NBP2-80727] - Etheno-ADP-ribosylation of cell-surface proteins on C3H-10T1/2 cells. C3H-10T 1/2 cells at different stages of differentiation (progenitors, MyoD-myoblasts, myotubes) were incubated for 30 minutes in the absence (dashed lines) or presence (solid lines) of 100 uM etheno-NAD. Cells were then washed, incubated with the primary monoclonal antibody 1G4, stained with a FITC-conjugated goat-anti-mouse secondary antibody and subjected to FACS analysis. Data show one representative experiments out of three. Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-91), licensed under a CC-BY license. Image from the standard format of this antibody.

• Reactivity: All
• Applications: WB, ELISA, Flow, ICC/IF, IP, CyTOF-ready
• Clone: 1G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Ethenoadenosine Antibody (1G4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Ethenoadenosine Antibody (1G4) - Azide and BSA Free (NB600-442) is a monoclonal antibody validated for use in WB, ELISA, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Ethenoadenosine
• Localization: Nuclear
• Isotype: IgG2 Lambda
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000. Use reported in scientific literature (PMID 9774627)
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:800
• Application Notes: This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Ethenoadenosine Antibody (1G4) - Azide and BSA Free

• C12H13N5O4
• N6-Ethenoadenosine

Background

ADP-ribosylation represents a posttranslational modification that involves transfer of ADP-ribose moiety from NAD+ to a specific amino acid in target protein (greatly modifying protein function) with the release of nicotinamide moiety. It was originally discovered as a mechanism by which potent bacterial toxins, such as Diphtheria toxin and Cholera toxin, modify and inactivate human target proteins, and these toxins were suggested to mimic mammalian endogenous ADP-ribosyltransferases. ART2 is an ecto-ADP-ribosyltransferases family (ART1-ART5) member which distantly resembles ADP-ribosylating bacterial toxins. It is expressed by mature T cells in which it ADP-ribosylates the integrin LFA-1 as well as other cell surface proteins, and this ART2-mediated ADP-ribosylation of T cell surface proteins provides a novel means for triggering T cell apoptosis. Ethenoadenosine moiety is contained in NAD analogue etheno-NAD which serves as an efficient substrate for ADP-ribosyltransferases and ethenoadenosine specific antibody clone 1G4 can be used for detection of ADP-ribosylated amino acids (ADP-ribosylation of cellular proteins) by FACS/FLOW, ICC-IF and Western blot applications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.