Human CXCL4/PF4 Quantikine ELISA Kit

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Human CXCL4/PF4 Quantikine ELISA Kit Summary

Background
The Quantikine Human CXCL4/PF4 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PF4 in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant human PF4 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PF4 showed linear curves that were parallel to the standard c...urves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PF4.
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Specificity
Natural and recombinant human PF4.
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
PF4

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DPF40.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CXCL4/PF4 Quantikine ELISA Kit

  • chemokine (C-X-C motif) ligand 4
  • C-X-C motif chemokine 4
  • CXCL4
  • CXCL4iroplact
  • Iroplact
  • MGC138298
  • Oncostatin-A
  • PF4
  • PF-4
  • platelet factor 4
  • SCYB4oncostatin-A

Background

CXCL4/PF4 is a secreted chemokine that is expressed by megakaryocytes and restrains the coagulation cascade. It is released by activated platelets and regulates Thrombin/Thrombomodulin complexes, activated Protein C (APC), Coagulation Factor Xa, and Fibrin. The anticoagulant heparin neutralizes CXCL4 procoagulant effects. Complexes of heparin and CXCL4 trigger antibody formation that causes the pathological syndrome HITT. Immunogenic complexes of CXCL4 with Apolipoprotein H contribute to antiphospholipid syndrome (APS). CXCL4 interferes with the proliferative and angiogenic functions of FGF-2 and VEGF. However, it can also exert proinflammatory and pro-atherogenic effects on monocytes, macrophages, and endothelial cells. CXCL4 may signal through CXCR3B in humans.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CXCL4/PF4 (DPF40)(11)

We have publications tested in 2 confirmed species: Human, Mouse.


Filter By Application
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Filter By Species
Human
(10)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using DPF40 Applications Species
M Ebeyer-Mas, T Eichhorn, R Weiss, V Semak, L Lauková, MB Fischer, V Weber Heparin-Functionalized Adsorbents Eliminate Central Effectors of Immunothrombosis, including Platelet Factor 4, High-Mobility Group Box 1 Protein and Histones International Journal of Molecular Sciences, 2022;23(3):. 2022 [PMID: 35163743] (Human) Human
S Nawrocka-M, J Biegus, M Hurkacz, M Guzik, M Rosiek-Bie, EA Jankowska, P Ponikowski, R Zymli?ski Differences in the Biomarker Profile of De Novo Acute Heart Failure versus Decompensation of Chronic Heart Failure Biomolecules, 2021;11(11):. 2021 [PMID: 34827701] (Human) Human
M Mussbacher, TL Krammer, S Heber, WC Schrottmai, S Zeibig, HP Holthoff, D Pereyra, P Starlinger, M Hackl, A Assinger Impact of Anticoagulation and Sample Processing on the Quantification of Human Blood-Derived microRNA Signatures Cells, 2020;9(8):. 2020 [PMID: 32824700] (Human) Human
M Mussbacher, TL Krammer, S Heber, WC Schrottmai, S Zeibig, HP Holthoff, D Pereyra, P Starlinger, M Hackl, A Assinger Impact of Anticoagulation and Sample Processing on the Quantification of Human Blood-Derived microRNA Signatures Cells, 2020;9(8):. 2020 [PMID: 32824700] (Human) Human
M Z?bczyk, J Natorska, A Janion-Sad, KP Malinowski, M Janion, A Undas Elevated Lactate Levels in Acute Pulmonary Embolism Are Associated with Prothrombotic Fibrin Clot Properties: Contribution of NETs Formation J Clin Med, 2020;9(4):. 2020 [PMID: 32235490] (Human) Human
CC Huang, CH Chou, SU Chen, HN Ho, YS Yang, MJ Chen Increased platelet factor 4 and aberrant permeability of follicular fluid in PCOS J. Formos. Med. Assoc., 2018;0(0):. 2018 [PMID: 29779926] (Human) Human
M Mussbacher, WC Schrottmai, M Salzmann, C Brostjan, JA Schmid, P Starlinger, A Assinger Optimized plasma preparation is essential to monitor platelet-stored molecules in humans PLoS ONE, 2017;12(12):e0188921. 2017 [PMID: 29220362] (Human) Human
Elizabeth R Volkmann Changes in plasma CXCL4 levels are associated with improvements in lung function in patients receiving immunosuppressive therapy for systemic sclerosis-related interstitial lung disease Arthritis Res. Ther, 2016;18(1):305. 2016 [PMID: 28038680] (Human) Human
Unaltered Angiogenesis-Regulating Activities of Platelets in Mild Type 2 Diabetes Mellitus despite a Marked Platelet Hyperreactivity PLoS ONE, 2016;11(9):e0162405. 2016 [PMID: 27612088] (Human) Human
Vir B Singh Smoothened Agonist Reduces Human Immunodeficiency Virus Type-1-Induced Blood-Brain Barrier Breakdown in Humanized Mice Sci Rep, 2016;6(0):26876. 2016 [PMID: 27241024] (Mouse) Mouse
Show All 11 Publications.

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Bioinformatics

Gene Symbol PF4