Human CXCL4/PF4 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human CXCL4/PF4 Quantikine ELISA Kit Summary

• Background: The Quantikine Human CXCL4/PF4 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PF4 in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant human PF4 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PF4 showed linear curves that were parallel to the standard c...urves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PF4.
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• Specificity: Natural and recombinant human PF4.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: PF4

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CXCL4/PF4 Quantikine ELISA Kit

• chemokine (C-X-C motif) ligand 4
• C-X-C motif chemokine 4
• CXCL4
• CXCL4iroplact
• Iroplact
• MGC138298
• Oncostatin-A
• PF4
• PF-4
• platelet factor 4
• SCYB4oncostatin-A

Background

CXCL4/PF4 is a secreted chemokine that is expressed by megakaryocytes and restrains the coagulation cascade. It is released by activated platelets and regulates Thrombin/Thrombomodulin complexes, activated Protein C (APC), Coagulation Factor Xa, and Fibrin. The anticoagulant heparin neutralizes CXCL4 procoagulant effects. Complexes of heparin and CXCL4 trigger antibody formation that causes the pathological syndrome HITT. Immunogenic complexes of CXCL4 with Apolipoprotein H contribute to antiphospholipid syndrome (APS). CXCL4 interferes with the proliferative and angiogenic functions of FGF-2 and VEGF. However, it can also exert proinflammatory and pro-atherogenic effects on monocytes, macrophages, and endothelial cells. CXCL4 may signal through CXCR3B in humans.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CXCL4/PF4 (DPF40)(19)

Publications using DPF40

• Sun, H;Chen, DW;Ma, YY;Liu, B;Wang, J;Lai, YJ;Zeng, GH;Shen, YY;Tan, CR;Bu, XL;Zeng, F;Wang, YJ; Decreased serum PF4 levels correlate with cognitive decline and CSF biomarkers in Alzheimer's disease in a Chinese cohort Experimental gerontology 2025-01-24 [PMID: 39855320] (Human)
• Guo, CY;Mo, R;Kim, H; Surface topography modulates initial platelet adhesion to titanium substrata Journal of oral biology and craniofacial research 2024-06-07 [PMID: 38962718] (Human)
• Mochizuki, T;Ushiki, T;Suzuki, K;Sato, M;Ishiguro, H;Suwabe, T;Watanabe, S;Edama, M;Omori, G;Yamamoto, N;Kawase, T; Elevated IL-1? and Comparable IL-1 Receptor Antagonist Levels Are Characteristic Features of L-PRP in Female College Athletes Compared to Male Professional Soccer Players International journal of molecular sciences 2023-12-14 [PMID: 38139317] (Human)
• Z?bczyk, M;Kruk, A;Natorska, J;Undas, A; Low-grade endotoxemia in acute pulmonary embolism: Links with prothrombotic plasma fibrin clot phenotype Thrombosis research 2023-12-01 [PMID: 37949000] (Human)
• Mochizuki, T;Ushiki, T;Suzuki, K;Sato, M;Ishiguro, H;Suwabe, T;Edama, M;Omori, G;Yamamoto, N;Kawase, T; Characterization of Leukocyte- and Platelet-Rich Plasma Derived from Female Collage Athletes: A Cross-Sectional Cohort Study Focusing on Growth Factor, Inflammatory Cytokines, and Anti-Inflammatory Cytokine Levels International journal of molecular sciences 2023-09-02 [PMID: 37686398] (Human)
• Stewart, JC;Patel, JS;Polanka, BM;Gao, S;Nurnberger, JI;MacDonald, KL;Gupta, SK;Considine, RV;Kovacs, RJ;Vrany, EA;Berntson, J;Hsueh, L;Shell, AL;Rollman, BL;Callahan, CM; Effect of Modernized Collaborative Care for Depression on Depressive Symptoms and Cardiovascular Disease Risk Biomarkers: eIMPACT Randomized Controlled Trial Brain, behavior, and immunity 2023-05-18 [PMID: 37209779] (Human)
• RO Ali, GM Quinn, R Umarova, JA Haddad, GY Zhang, EC Townsend, L Scheuing, KL Hill, M Gewirtz, S Rampertaap, SD Rosenzweig, AT Remaley, JM Han, V Periwal, H Cai, PJ Walter, C Koh, EB Levy, DE Kleiner, O Etzion, T Heller Longitudinal multi-omics analyses of the gut-liver axis reveals metabolic dysregulation in hepatitis C infection and cirrhosis Nature Microbiology, 2022-12-15;0(0):. 2022-12-15 [PMID: 36522461] (Human)
• C Tacquard, C Mouriaux, X Delabranch, C Bourdon, A Eckly, S Magnenat, L Sattler, C Gachet, PM Mertes, B Hechler, PH Mangin Platelet dysfunction and thrombus instability in flow conditions in patients with severe COVID-19 Thrombosis Research, 2022-11-08;0(0):. 2022-11-08 [PMID: 36376109] (Human)
• H Kared, AS Wolf, A Alirezayla, A Ravussin, G Solum, TT Tran, F Lund-Johan, JT Vaage, LS Nissen-Mey, UC Nygaard, O Hungnes, AH Robertson, LM Næss, L Trogstad, P Magnus, LA Munthe, S Mjaaland Immune responses in Omicron SARS-CoV-2 breakthrough infection in vaccinated adults Nature Communications, 2022-07-18;13(1):4165. 2022-07-18 [PMID: 35851055] (Human)
• M Ebeyer-Mas, T Eichhorn, R Weiss, V Semak, L Lauková, MB Fischer, V Weber Heparin-Functionalized Adsorbents Eliminate Central Effectors of Immunothrombosis, including Platelet Factor 4, High-Mobility Group Box 1 Protein and Histones International Journal of Molecular Sciences, 2022-02-05;23(3):. 2022-02-05 [PMID: 35163743] (Human)
• S Nawrocka-M, J Biegus, M Hurkacz, M Guzik, M Rosiek-Bie, EA Jankowska, P Ponikowski, R Zymli?ski Differences in the Biomarker Profile of De Novo Acute Heart Failure versus Decompensation of Chronic Heart Failure Biomolecules, 2021-11-16;11(11):. 2021-11-16 [PMID: 34827701] (Human)
• M Mussbacher, TL Krammer, S Heber, WC Schrottmai, S Zeibig, HP Holthoff, D Pereyra, P Starlinger, M Hackl, A Assinger Impact of Anticoagulation and Sample Processing on the Quantification of Human Blood-Derived microRNA Signatures Cells, 2020-08-18;9(8):. 2020-08-18 [PMID: 32824700] (Human)
• M Z?bczyk, J Natorska, A Janion-Sad, KP Malinowski, M Janion, A Undas Elevated Lactate Levels in Acute Pulmonary Embolism Are Associated with Prothrombotic Fibrin Clot Properties: Contribution of NETs Formation J Clin Med, 2020-03-30;9(4):. 2020-03-30 [PMID: 32235490] (Human)
• CC Huang, CH Chou, SU Chen, HN Ho, YS Yang, MJ Chen Increased platelet factor 4 and aberrant permeability of follicular fluid in PCOS J. Formos. Med. Assoc., 2018-05-18;0(0):. 2018-05-18 [PMID: 29779926] (Human)
• M Mussbacher, WC Schrottmai, M Salzmann, C Brostjan, JA Schmid, P Starlinger, A Assinger Optimized plasma preparation is essential to monitor platelet-stored molecules in humans PLoS ONE, 2017-12-08;12(12):e0188921. 2017-12-08 [PMID: 29220362] (Human)
• Elizabeth R Volkmann Changes in plasma CXCL4 levels are associated with improvements in lung function in patients receiving immunosuppressive therapy for systemic sclerosis-related interstitial lung disease Arthritis Res. Ther, 2016-12-30;18(1):305. 2016-12-30 [PMID: 28038680] (Human)
• Unaltered Angiogenesis-Regulating Activities of Platelets in Mild Type 2 Diabetes Mellitus despite a Marked Platelet Hyperreactivity PLoS ONE, 2016-09-09;11(9):e0162405. 2016-09-09 [PMID: 27612088] (Human)
• Vir B Singh Smoothened Agonist Reduces Human Immunodeficiency Virus Type-1-Induced Blood-Brain Barrier Breakdown in Humanized Mice Sci Rep, 2016-05-31;6(0):26876. 2016-05-31 [PMID: 27241024] (Mouse)
• Proenca-Ferreira R, Brugnerotto A, Garrido V, Dominical V, Vital D, Ribeiro M, dos Santos M, Traina F, Olalla-Saad S, Costa F, Conran N Endothelial activation by platelets from sickle cell anemia patients. PLoS ONE, 2014-02-13;9(2):e89012. 2014-02-13 [PMID: 24551209] (Human)

Bioinformatics

• Gene Symbol: PF4