Ethenoadenosine Antibody (1G4)


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Product Details

Reactivity AllSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IP, CyTOF-ready
1.2 mg/ml

Ethenoadenosine Antibody (1G4) Summary

This is specific for ethenoadenosine and ethenodadenosine.
IgG2 Lambda
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:800
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
This Ethenoadenosine antibody is useful for Flow Cytometry (PMID 9774627), Immunocytochemistry/Immunofluorescence, Immunoprecipitation, ELISA and Western blot.
Read Publications using
NB600-442 in the following applications:

  • 9 publications
  • 6 publications
  • WB
    3 publications

Reactivity Notes

All species.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1% Sodium Azide
1.2 mg/ml
Protein G purified


ADP-ribosylation represents a posttranslational modification that involves transfer of ADP-ribose moiety from NAD+ to a specific amino acid in target protein (greatly modifying protein function) with the release of nicotinamide moiety. It was originally discovered as a mechanism by which potent bacterial toxins, such as Diphtheria toxin and Cholera toxin, modify and inactivate human target proteins, and these toxins were suggested to mimic mammalian endogenous ADP-ribosyltransferases. ART2 is an ecto-ADP-ribosyltransferases family (ART1-ART5) member which distantly resembles ADP-ribosylating bacterial toxins. It is expressed by mature T cells in which it ADP-ribosylates the integrin LFA-1 as well as other cell surface proteins, and this ART2-mediated ADP-ribosylation of T cell surface proteins provides a novel means for triggering T cell apoptosis. Ethenoadenosine moiety is contained in NAD analogue etheno-NAD which serves as an efficient substrate for ADP-ribosyltransferases and ethenoadenosine specific antibody clone 1G4 can be used for detection of ADP-ribosylated amino acids (ADP-ribosylation of cellular proteins) by FACS/FLOW, ICC-IF and Western blot applications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ethenoadenosine Antibody (NB600-442)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 3 applications: FLOW, ICC/IF, WB.

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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB600-442 Applications Species
Friedrich M, Bohlig L, Kirschner RD, EngelK, Hauschildt S. Identification of two regulatory binding sites which confer myotube specific expression of the mono-ADP-ribosyltransferase ART1 gene. BMC Mol Biol;9:91. 2008 Oct 21. [PMID: 18939989] (FLOW ) FLOW
Grahnert A, Richter S, Siegert F, Berndt A, Hauschildt S. The orthologue of the acatalytic mammalian ART4 in chicken is an arginine-specific mono-ADP-ribosyltransferase. BMC Mol Biol;9:86. 2008 Oct 14. [PMID: 18854029] (WB, FLOW, Human, Chicken) WB, FLOW Human, Chicken
Hong S, Brass A, Seman M et al. Lipopolysaccharide, IFN-gamma, and IFN-beta induce expression of the thiol-sensitive ART2.1 Ecto-ADP-ribosyltransferase in murine macrophages. J Immunol. 2007 Nov 1 [PMID: 17947697]
Koch-Nolte F, Reyelt J, Schossow B, Schwarz N, Scheuplein F, Rothenburg S, Haag F, Alzogaray V, Cauerhff A, Goldbaum FA. Single domain antibodies from llama effectively and specifically block T cell ecto-ADP-ribosyltransferase ART2.2 in vivo. FASEB J. 21(13):3490-8. 2007 Nov. [PMID: 17575259] (ICC/IF, Mouse) ICC/IF Mouse
Liao, SD, Puro, DG. NAD+-induced vasotoxicity in the pericyte-containing microvasculature of the rat retina: effect of diabetes. Invest Ophthalmol Vis Sci;47(11):5032-8. 2006 Nov. [PMID: 17065524] (ICC/IF, Rat) ICC/IF Rat
Kawamura H, Aswad F, Minagawa M, Govindarajan S, Dennert G. P2X7 receptors regulate NKT cells in autoimmune hepatitis. J Immunol;176(4):2152-60. 2006 Feb 15. [PMID: 16455971]
Aswad F, Kawamura H, Dennert G. High sensitivity of CD4+CD25+ regulatory T cells to extracellular metabolites nicotinamide adenine dinucleotide and ATP: a role for P2X7 receptors. J Immunol;175(5):3075-83. 2005 Sep 1. [PMID: 16116196] (FLOW ) FLOW
Kawamura H, Aswad F, Minagawa M, Malone K, Kaslow H, Koch-Nolte F, Schott WH, Leiter EH, Dennert G. P2X7 receptor-dependent and -independent T cell death is induced by nicotinamide adenine dinucleotide. J Immunol;174(4):1971-9. 2005 Feb 15. [PMID: 15699125] (WB, FLOW, Mouse) WB, FLOW Mouse
Krebs, C et al. Flow cytometric and immunoblot assays for cell surface ADP-ribosylation using a monoclonal antibody specific for ethenoadenosine. Anal Biochem;314(1):108-15. 2003 Mar 1. [PMID: 12633608] (WB, FLOW, ICC/IF, Human, Mouse) WB, FLOW, ICC/IF Human, Mouse
Ohlrogge, W et al. Generation and characterization of ecto-ADP-ribosyltransferase ART2.1/ART2.2-deficient mice. Mol Cell Biol. 22(21):7535-42. 2002 Nov. [PMID: 12370300]
Show All 15 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-442AF405
Alexa Fluor 488 Labeled NB600-442AF488
Alexa Fluor 647 Labeled NB600-442AF647
Alexa Fluor 700 Labeled NB600-442AF700
Allophycocyanin Labeled NB600-442APC
Biotin Labeled NB600-442B
DyLight 350 Labeled NB600-442UV
DyLight 405 Labeled NB600-442V
DyLight 488 Labeled NB600-442G
DyLight 550 Labeled NB600-442R
DyLight 650 Labeled NB600-442C
DyLight 680 Labeled NB600-442FR
DyLight 755 Labeled NB600-442IR
FITC Labeled NB600-442F
HRP Labeled NB600-442H
PE Labeled NB600-442PE
PerCP Labeled NB600-442PCP

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Ethenoadenosine NB600-442

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