Ethenoadenosine Antibody (1G4)

Images

 
There are currently no images for Ethenoadenosine Antibody (NB600-442).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IP, CyTOF-ready
Clone
1G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.2 mg/ml

Ethenoadenosine Antibody (1G4) Summary

Immunogen
Ethenoadenosine
Localization
Nuclear
Specificity
This is specific for ethenoadenosine and ethenodadenosine.
Isotype
IgG2 Lambda
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:800
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
This Ethenoadenosine antibody is useful for Flow Cytometry (PMID 9774627), Immunocytochemistry/Immunofluorescence, Immunoprecipitation, ELISA and Western blot.
Publications
Read Publications using
NB600-442 in the following applications:

  • 9 publications
  • 6 publications
  • WB
    3 publications

Reactivity Notes

All species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.2 mg/ml
Purity
Protein G purified

Background

ADP-ribosylation represents a posttranslational modification that involves transfer of ADP-ribose moiety from NAD+ to a specific amino acid in target protein (greatly modifying protein function) with the release of nicotinamide moiety. It was originally discovered as a mechanism by which potent bacterial toxins, such as Diphtheria toxin and Cholera toxin, modify and inactivate human target proteins, and these toxins were suggested to mimic mammalian endogenous ADP-ribosyltransferases. ART2 is an ecto-ADP-ribosyltransferases family (ART1-ART5) member which distantly resembles ADP-ribosylating bacterial toxins. It is expressed by mature T cells in which it ADP-ribosylates the integrin LFA-1 as well as other cell surface proteins, and this ART2-mediated ADP-ribosylation of T cell surface proteins provides a novel means for triggering T cell apoptosis. Ethenoadenosine moiety is contained in NAD analogue etheno-NAD which serves as an efficient substrate for ADP-ribosyltransferases and ethenoadenosine specific antibody clone 1G4 can be used for detection of ADP-ribosylated amino acids (ADP-ribosylation of cellular proteins) by FACS/FLOW, ICC-IF and Western blot applications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ethenoadenosine Antibody (NB600-442)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 3 applications: FLOW, ICC/IF, WB.


Filter By Application
FLOW
(9)
ICC/IF
(6)
WB
(3)
All Applications
Filter By Species
Human
(4)
Mouse
(5)
Rat
(1)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB600-442 Applications Species
Friedrich M, Bohlig L, Kirschner RD, EngelK, Hauschildt S. Identification of two regulatory binding sites which confer myotube specific expression of the mono-ADP-ribosyltransferase ART1 gene. BMC Mol Biol;9:91. 2008 Oct 21. [PMID: 18939989] (FLOW ) FLOW
Grahnert A, Richter S, Siegert F, Berndt A, Hauschildt S. The orthologue of the acatalytic mammalian ART4 in chicken is an arginine-specific mono-ADP-ribosyltransferase. BMC Mol Biol;9:86. 2008 Oct 14. [PMID: 18854029] (WB, FLOW, Human, Chicken) WB, FLOW Human, Chicken
Hong S, Brass A, Seman M et al. Lipopolysaccharide, IFN-gamma, and IFN-beta induce expression of the thiol-sensitive ART2.1 Ecto-ADP-ribosyltransferase in murine macrophages. J Immunol. 2007 Nov 1 [PMID: 17947697]
Koch-Nolte F, Reyelt J, Schossow B, Schwarz N, Scheuplein F, Rothenburg S, Haag F, Alzogaray V, Cauerhff A, Goldbaum FA. Single domain antibodies from llama effectively and specifically block T cell ecto-ADP-ribosyltransferase ART2.2 in vivo. FASEB J. 21(13):3490-8. 2007 Nov. [PMID: 17575259] (ICC/IF, Mouse) ICC/IF Mouse
Liao, SD, Puro, DG. NAD+-induced vasotoxicity in the pericyte-containing microvasculature of the rat retina: effect of diabetes. Invest Ophthalmol Vis Sci;47(11):5032-8. 2006 Nov. [PMID: 17065524] (ICC/IF, Rat) ICC/IF Rat
Kawamura H, Aswad F, Minagawa M, Govindarajan S, Dennert G. P2X7 receptors regulate NKT cells in autoimmune hepatitis. J Immunol;176(4):2152-60. 2006 Feb 15. [PMID: 16455971]
Aswad F, Kawamura H, Dennert G. High sensitivity of CD4+CD25+ regulatory T cells to extracellular metabolites nicotinamide adenine dinucleotide and ATP: a role for P2X7 receptors. J Immunol;175(5):3075-83. 2005 Sep 1. [PMID: 16116196] (FLOW ) FLOW
Kawamura H, Aswad F, Minagawa M, Malone K, Kaslow H, Koch-Nolte F, Schott WH, Leiter EH, Dennert G. P2X7 receptor-dependent and -independent T cell death is induced by nicotinamide adenine dinucleotide. J Immunol;174(4):1971-9. 2005 Feb 15. [PMID: 15699125] (WB, FLOW, Mouse) WB, FLOW Mouse
Krebs, C et al. Flow cytometric and immunoblot assays for cell surface ADP-ribosylation using a monoclonal antibody specific for ethenoadenosine. Anal Biochem;314(1):108-15. 2003 Mar 1. [PMID: 12633608] (WB, FLOW, ICC/IF, Human, Mouse) WB, FLOW, ICC/IF Human, Mouse
Ohlrogge, W et al. Generation and characterization of ecto-ADP-ribosyltransferase ART2.1/ART2.2-deficient mice. Mol Cell Biol. 22(21):7535-42. 2002 Nov. [PMID: 12370300]
Show All 15 Publications.

Reviews for Ethenoadenosine Antibody (NB600-442) (0)

There are no reviews for Ethenoadenosine Antibody (NB600-442). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for Ethenoadenosine Antibody (NB600-442): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ethenoadenosine Antibody (NB600-442) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-442AF405
Alexa Fluor 488 Labeled NB600-442AF488
Alexa Fluor 647 Labeled NB600-442AF647
Alexa Fluor 700 Labeled NB600-442AF700
Allophycocyanin Labeled NB600-442APC
Biotin Labeled NB600-442B
DyLight 350 Labeled NB600-442UV
DyLight 405 Labeled NB600-442V
DyLight 488 Labeled NB600-442G
DyLight 550 Labeled NB600-442R
DyLight 650 Labeled NB600-442C
DyLight 680 Labeled NB600-442FR
DyLight 755 Labeled NB600-442IR
FITC Labeled NB600-442F
HRP Labeled NB600-442H
PE Labeled NB600-442PE
PerCP Labeled NB600-442PCP

Additional Ethenoadenosine Products

Ethenoadenosine NB600-442

Research Areas for Ethenoadenosine Antibody (NB600-442)

Find related products by research area.

Blogs on Ethenoadenosine

There are no specific blogs for Ethenoadenosine, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Ethenoadenosine Antibody (1G4) and receive a gift card or discount.