CaM Kinase II alpha Antibody (6G9)

Images

 
Western Blot: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - analysis of Rat brain membrane lysate showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Primary Antibody: Mouse ...read more
Immunocytochemistry/ Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: dissociated hippocampal neurons. Species: Mouse. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium ...read more
Immunohistochemistry: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:10000 for 12 hours at 4 ...read more
Immunocytochemistry/ Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degrees C. Primary Antibody: Mouse ...read more
Immunocytochemistry/ Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, XpSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP, RIA
Clone
6G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates

CaM Kinase II alpha Antibody (6G9) Summary

Immunogen
Partially purified rat CaMKII
Localization
Cytoplasm, Mitochondrion, Nucleus, Cell Junction, Synapse, Presynaptic Cell Membrane
Specificity
Detects approx 50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CAMK2A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500RadioImmunoassay 1:100-1:2000
  • Radioimmunoassay 1:100-1:2000
Application Notes
0.1 ug/ml was sufficient for detection of CamKII in 20 ug rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Rat Brain Whole Tissue Lysate (Adult Whole Normal)
Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NB100-1983 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • WB
    4 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28421537). Xenopus reactivity reported in scientific literature (PMID: 29412139). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CaM Kinase II alpha Antibody (6G9)

  • calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
  • calcium/calmodulin-dependent protein kinase II alpha
  • calcium/calmodulin-dependent protein kinase II alpha-B subunit
  • CaM kinase II alpha subunit
  • CaM Kinase II alpha
  • CaM kinase II subunit alpha
  • CAMK2A
  • CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
  • CaMK-II alpha subunit
  • CaMK-II subunit alpha
  • CaMKIINalpha
  • CaM-kinase II alpha chain
  • EC 2.7.11
  • EC 2.7.11.17
  • KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Background

CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1983
Species: Hu, Mu, Rt, Bv, Xp
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, RIA

Publications for CaM Kinase II alpha Antibody (NB100-1983)(9)

We have publications tested in 3 confirmed species: Mouse, Rat, Xenopus.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
WB
(4)
All Applications
Filter By Species
Mouse
(3)
Rat
(2)
Xenopus
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-1983 Applications Species
Schiapparelli LM, Shah SH, Ma Y et al. The Retinal Ganglion Cell Transportome Identifies Proteins Transported to Axons and Presynaptic Compartments in the Visual System In Vivo Cell Rep Aug 13 2019 [PMID: 31412257] (WB, Rat) WB Rat
Koene LMC, van Grondelle SE, Proietti Onori M et al. Effects of antiepileptic drugs in a new TSC/mTOR-dependent epilepsy mouse model Ann Clin Transl Neurol Jul 1 2019 [PMID: 31353861] (IHC, Mouse) IHC Mouse
Fang Q, Li Z, Huang GD et al. Traumatic Stress Produces Distinct Activations of GABAergic and Glutamatergic Neurons in Amygdala. Front Neurosci Aug 21 2018 [PMID: 30186100] (ICC/IF, Rat) ICC/IF Rat
Liu HH, McClatchy DB, Schiapparelli L, Shen W. Role of the visual experience-dependent nascent proteome in neuronal plasticity. Elife. 2018 Feb 07 [PMID: 29412139] (WB, Xenopus) WB Xenopus
Li Y, You QL, Zhang SR et al. Satb2 Ablation Impairs Hippocampus-Based Long-Term Spatial Memory and Short-Term Working Memory and Immediate Early Genes (IEGs)-Mediated Hippocampal Synaptic Plasticity. Mol. Neurobiol. Apr 18 2017 [PMID: 28421537] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Hamada S, Ogawa I, Yamasaki M et al. The glutamate receptor GluN2 subunit regulates synaptic trafficking of AMPA receptors in the neonatal mouse brain Eur. J. Neurosci. 2014 Aug 08 [PMID: 25131300]
Balu Darrick T, Takagi Shunsuke, Puhl Matthew D et al. D-serine and serine racemase are localized to neurons in the adult mouse and human forebrain. Cell Mol Neurobiol. 2014 Jan 17 [PMID: 24436034]
George J, Baden DG, Gerwick WH, Murray TF. Bidirectional influence of sodium channel activation on NMDA receptor-dependent cerebrocortical neuron structural plasticity Proc Natl Acad Sci U S A 2012 Nov 27 [PMID: 23150561] (WB, Mouse) WB Mouse
Tianshu Feng, Eva Szabo, Ewa Dziak Michal Opas. Cytoskeletal Disassembly and Cell Rounding Promotes Adipogenesis from ES Cells. Stem Cell Reviews and Reports Volume 6, Number 1, 74-86. [PMID: 20148318]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CaM Kinase II alpha Antibody (NB100-1983). (Showing 1 - 1 of 1 FAQs).

  1. I purchased the CaMKII alpha Antibody (6G9) (Catalog number NB100-1983). It worked well for WB, but it does not work well for IHC on frozen section. I would like to know whether it can be used for IHC-Fr.
    • Our CaMKII alpha antibody with catalogue number NB100-1983 has not been tested for IHC with frozen tissue, and so I am not certain that it will work in this application.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin NB100-1983B
DyLight 550 NB100-1983R
DyLight 650 NB100-1983C
FITC NB100-1983F
HRP NB100-1983H

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Bioinformatics

Gene Symbol CAMK2A
Entrez
Uniprot
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