Western Blot: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - analysis of Rat brain membrane lysate showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Primary Antibody: Mouse ...read more
Immunocytochemistry/ Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: dissociated hippocampal neurons. Species: Mouse. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium ...read more
Immunohistochemistry: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:10000 for 12 hours at 4 ...read more
Immunocytochemistry/ Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degrees C. Primary Antibody: Mouse ...read more
Immunocytochemistry/ Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII ...read more
0.1 ug/ml was sufficient for detection of CamKII in 20 ug rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
54 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Mouse reactivity reported in scientific literature (PMID: 28421537). Xenopus reactivity reported in scientific literature (PMID: 29412139). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4) and 50% Glycerol
0.09% Sodium Azide
Protein G purified
Alternate Names for CaM Kinase II alpha Antibody (6G9)
calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
calcium/calmodulin-dependent protein kinase II alpha
calcium/calmodulin-dependent protein kinase II alpha-B subunit
CaM kinase II alpha subunit
CaM Kinase II alpha
CaM kinase II subunit alpha
CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
CaMK-II alpha subunit
CaMK-II subunit alpha
CaM-kinase II alpha chain
KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain
CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CaM Kinase II alpha Antibody (NB100-1983). (Showing 1 - 1 of 1 FAQs).
I purchased the CaMKII alpha Antibody (6G9) (Catalog number NB100-1983). It worked well for WB, but it does not work well for IHC on frozen section. I would like to know whether it can be used for IHC-Fr.
Our CaMKII alpha antibody with catalogue number NB100-1983 has not been tested for IHC with frozen tissue, and so I am not certain that it will work in this application.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our CaM Kinase II alpha Antibody (6G9) and receive a gift card or discount.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.