Human S100A8/S100A9 Heterodimer DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human S100A8/S100A9 Heterodimer DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: S100A8

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human S100A8/S100A9 Heterodimer DuoSet ELISA, 5 Plate

• 60B8AG
• CAGA
• calgranulin A
• Calprotectin L1L subunit
• Calprotectin
• CFAG
• CGLA
• CP-10
• Cystic fibrosis antigen
• L1AG
• Leukocyte L1 complex light chain
• MA387
• MIF
• Migration inhibitory factor-related protein 8
• MRP8
• NIF
• p8
• protein S100-A8
• S100-A8
• S100A8/S100A9 Heterodimer
• Urinary stone protein band A

Background

S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca²⁺-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca²⁺-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Publications for S100A8/S100A9 Heterodimer (DY8226-05)(20)

Publications using DY8226-05

• Cabrera, LE;Jokiranta, ST;Mäki, S;Miettinen, S;Kant, R;Kareinen, L;Sironen, T;Pietilä, JP;Kantele, A;Kekäläinen, E;Lindgren, H;Mattila, P;Kipar, A;Vapalahti, O;Strandin, T; The assembly of neutrophil inflammasomes during COVID-19 is mediated by type I interferons PLoS pathogens 2024-08-01 [PMID: 39172744] (Human)
• Kortam, N;Liang, W;Shiple, C;Huang, S;Gedert, R;Clair, JS;Sarosh, C;Foster, C;Tsou, PS;Varga, J;Knight, JS;Khanna, D;Ali, RA; Elevated neutrophil extracellular traps in systemic sclerosis-associated vasculopathy and suppression by a synthetic prostacyclin analog Arthritis research & therapy 2024-07-25 [PMID: 39054558] (Human)
• Barbu, E;Mihaila, AC;Gan, AM;Ciortan, L;Macarie, RD;Tucureanu, MM;Filippi, A;Stoenescu, AI;Petrea, SV;Simionescu, M;Balanescu, SM;Butoi, E; The Elevated Inflammatory Status of Neutrophils Is Related to In-Hospital Complications in Patients with Acute Coronary Syndrome and Has Important Prognosis Value for Diabetic Patients International journal of molecular sciences 2024-05-08 [PMID: 38791147] (Human)
• Chick, HM;Rees, ME;Lewis, ML;Williams, LK;Bodger, O;Harris, LG;Rushton, S;Wilkinson, TS; Using the Traditional Ex Vivo Whole Blood Model to Discriminate Bacteria by Their Inducible Host Responses Biomedicines 2024-03-25 [PMID: 38672079] (Human)
• Chung, YH;Ortega-Rivera, OA;Volckaert, BA;Jung, E;Zhao, Z;Steinmetz, NF; Viral nanoparticle vaccines against S100A9 reduce lung tumor seeding and metastasis Proceedings of the National Academy of Sciences of the United States of America 2023-10-24 [PMID: 37844250] (Mouse)
• Ali, RA;Minarchick, VC;Zahavi, M;Rysenga, CE;Sturm, KA;Hoy, CK;Sarosh, C;Knight, JS;Demoruelle, MK; Ginger intake suppresses neutrophil extracellular trap formation in autoimmune mice and healthy humans JCI insight 2023-09-22 [PMID: 37737262] (Human)
• Woodruff, MC;Bonham, KS;Anam, FA;Walker, TA;Faliti, CE;Ishii, Y;Kaminski, CY;Ruunstrom, MC;Cooper, KR;Truong, AD;Dixit, AN;Han, JE;Ramonell, RP;Haddad, NS;Rudolph, ME;Yalavarthi, S;Betin, V;Natoli, T;Navaz, S;Jenks, SA;Zuo, Y;Knight, JS;Khosroshahi, A;Lee, FE;Sanz, I; Chronic inflammation, neutrophil activity, and autoreactivity splits long COVID Nature communications 2023-07-14 [PMID: 37452024] (Human)
• Flerlage, T;Crawford, JC;Allen, EK;Severns, D;Tan, S;Surman, S;Ridout, G;Novak, T;Randolph, A;West, AN;Thomas, PG; Single cell transcriptomics identifies distinct profiles in pediatric acute respiratory distress syndrome Nature communications 2023-06-30 [PMID: 37391405] (Human)
• L Chen, V Shi, S Wang, R Freeman, F Ruiz, K Jayachandr, J Zhang, P Cosper, L Sun, CJ Luke, C Spina, PW Grigsby, JK Schwarz, S Markovina SCCA1/SERPINB3 promotes suppressive immune environment via STAT-dependent chemokine production, blunting the therapy-induced T cell responses bioRxiv : the preprint server for biology, 2023-02-03;0(0):. 2023-02-03 [PMID: 36778224] (Human)
• P Konstanti, M van Splunt, E van den Br, C Belzer, A Nauta, RJJ van Neerve, H Smidt The Effect of Nutritional Intervention with Lactoferrin, Galactooligosacharides and Vitamin D on the Gut Microbiota Composition of Healthy Elderly Women Nutrients, 2022-06-14;14(12):. 2022-06-14 [PMID: 35745198] (Human)
• Frederik Denorme, Irina Portier, John L. Rustad, Mark J. Cody, Claudia V. de Araujo, Chieko Hoki et al. Neutrophil extracellular traps regulate ischemic stroke brain injury Journal of Clinical Investigation 2022-05-16 [PMID: 35358095] (Human)
• S Hong, KH Park, YE Lee, JE Lee, YM Kim, E Joo, I Cho Antibody microarray analysis of amniotic fluid proteomes in women with cervical insufficiency and short cervix, and their association with pregnancy latency length PLoS ONE, 2022-02-07;17(2):e0263586. 2022-02-07 [PMID: 35130326] (Human)
• S Hakki, EJ Robinson, MG Robson Circulating Interleukin-6 and CD16 positive monocytes increase following angioplasty of an arteriovenous fistula Scientific Reports, 2022-01-26;12(1):1427. 2022-01-26 [PMID: 35082332] (Human)
• H Xiang, F Guo, X Tao, Q Zhou, S Xia, D Deng, L Li, D Shang Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation Theranostics, 2021-03-04;11(9):4467-4482. 2021-03-04 [PMID: 33754072] (Human)
• Y Zuo, M Warnock, A Harbaugh, S Yalavarthi, K Gockman, M Zuo, JA Madison, JS Knight, Y Kanthi, DA Lawrence Plasma tissue plasminogen activator and plasminogen activator inhibitor-1 in hospitalized COVID-19 patients Scientific Reports, 2021-01-15;11(1):1580. 2021-01-15 [PMID: 33452298] (Human)
• HJ Kim, KH Park, YM Kim, E Joo, K Ahn, S Shin A protein microarray analysis of amniotic fluid proteins for the prediction of spontaneous preterm delivery in women with preterm premature rupture of membranes at 23 to 30 weeks of gestation PLoS ONE, 2020-12-31;15(12):e0244720. 2020-12-31 [PMID: 33382822] (Human)
• Y Zuo, SK Estes, AA Gandhi, S Yalavarthi, RA Ali, H Shi, G Sule, K Gockman, JA Madison, M Zuo, W Woodard, SP Lezak, NL Lugogo, Y Kanthi, JS Knight Prothrombotic antiphospholipid antibodies in COVID-19 medRxiv, 2020-09-15;0(0):. 2020-09-15 [PMID: 32587992] (Human)
• H Shi, Y Zuo, S Yalavarthi, K Gockman, M Zuo, JA Madison, CN Blair, W Woodard, SP Lezak, NL Lugogo, RJ Woods, C Lood, JS Knight, Y Kanthi Neutrophil calprotectin identifies severe pulmonary disease in COVID-19 medRxiv, 2020-07-15;0(0):. 2020-07-15 [PMID: 32511540] (Human)
• YJ Park, SJ Woo, YM Kim, S Hong, YE Lee, KH Park Immune and Inflammatory Proteins in Cord Blood as Predictive Biomarkers of Retinopathy of Prematurity in Preterm Infants Invest. Ophthalmol. Vis. Sci., 2019-09-03;60(12):3813-3820. 2019-09-03 [PMID: 31525777] (Human)
• R Lande, EY Lee, R Palazzo, B Marinari, I Pietrafort, GS Santos, Y Mattenberg, F Spadaro, K Stefananto, N Iannace, AM Dufour, M Falchi, M Bianco, E Botti, L Bianchi, M Alvarez, V Riccieri, ME Truchetet, G C L Wong, C Chizzolini, L Frasca CXCL4 assembles DNA into liquid crystalline complexes to amplify TLR9-mediated interferon-? production in systemic sclerosis Nat Commun, 2019-05-01;10(1):1731. 2019-05-01 [PMID: 31043596] (Human)

Bioinformatics

• Gene Symbol: S100A8