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Porcine IL-10 Quantikine ELISA Kit

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Porcine IL-10 Quantikine ELISA Kit Summary

Background
The Quantikine Porcine IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-10 levels in cell culture supernates, porcine serum, and EDTA plasma. It contains E. coli-expressed recombinant porcine IL-10 and antibodies raised against the recombinant protein. Results obtained for naturally occurring porcine IL-10 showed linear curves that were parallel to th...e standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural porcine IL-10.
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Specificity
Natural and recombinant porcine IL-10
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Protein/Peptide Type
Peptide
Gene
IL10

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
P1000 in the following applications:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Preservative
No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Porcine IL-10 Quantikine ELISA Kit

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-10 (P1000)(18)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 1 application: ELISA Capture.


Filter By Application
ELISA Capture
(1)
All Applications
Filter By Species
Porcine
(18)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using P1000 Applications Species
Lin, S;Panthi, S;Hsuuw, Y;Chen, S;Huang, M;Sieber, M;Hsuuw, Y; Regenerative Effect of Mesenchymal Stem Cell on Cartilage Damage in a Porcine Model Biomedicines 2023-01-01 [PMID: 37509451] (Porcine) Porcine
A Madapong, K Saeng-Chut, A Tantituvan, D Nilubol Using a concurrent challenge with porcine circovirus 2 and porcine reproductive and respiratory syndrome virus to compare swine vaccination programs Scientific Reports, 2022-09-15;12(1):15524. 2022-09-15 [PMID: 36109529] (Porcine) Porcine
P Chaikhumwa, A Madapong, K Saeng-Chut, D Nilubol, A Tantituvan Intranasal delivery of inactivated PRRSV loaded cationic nanoparticles coupled with enterotoxin subunit B induces PRRSV-specific immune responses in pigs Scientific Reports, 2022-03-08;12(1):3725. 2022-03-08 [PMID: 35260663] (Porcine) Porcine
M Sponchiado, YS Liao, KR Atanasova, EN Collins, V Schurmann, L Bravo, LR Reznikov Overexpression of Substance P in pig airways increases MUC5AC through an NF-k&beta pathway Physiological Reports, 2021-02-01;9(3):e14749. 2021-02-01 [PMID: 33580593] (Porcine) Porcine
S Halvachiza, M Teuben, M Lempert, Y Kalbas, N Cesarovic, M Lipiski, E Benninger, P Cinelli, R Pfeifer, HC Pape Protective effects of new femoral reaming techniques (Reamer irrigator aspirator, RIA I and II) on pulmonary function and posttraumatic contusion (CT morphology) - results from a standardized large animal model Injury, 2020-10-06;0(0):. 2020-10-06 [PMID: 33046248] (Porcine) Porcine
D Desmonts d, R Moges, A Siddiq, T Allain, TD Feener, GP Muench, N McKenna, RM Yates, AG Buret Immuno-modulating properties of Tulathromycin in porcine monocyte-derived macrophages infected with porcine reproductive and respiratory syndrome virus PLoS ONE, 2019-08-23;14(8):e0221560. 2019-08-23 [PMID: 31442273] (Porcine) Porcine
V Kloubert, K Blaabjerg, TS Dalgaard, HD Poulsen, L Rink, I Wessels Influence of zinc supplementation on immune parameters in weaned pigs J Trace Elem Med Biol, 2018-01-31;0(0):. 2018-01-31 [PMID: 29402502] (ELISA Capture, Porcine) ELISA Capture Porcine
W Gozdzik, S Zielinski, M Zielinska, K Ratajczak, P Skrzypczak, S Rodziewicz, A Kübler, K Löfström, P Dziegiel, M Olbromski, B Adamik, S Ryniak, P Harbut, J Albert, C Frostell Beneficial effects of inhaled nitric oxide with intravenous steroid in an ischemia-reperfusion model involving aortic clamping Int J Immunopathol Pharmacol, 2018-01-01;32(0):3946320177514. 2018-01-01 [PMID: 29376749] (Porcine) Porcine
H Yan, KM Ajuwon Butyrate modifies intestinal barrier function in IPEC-J2 cells through a selective upregulation of tight junction proteins and activation of the Akt signaling pathway PLoS ONE, 2017-06-27;12(6):e0179586. 2017-06-27 [PMID: 28654658] (Porcine) Porcine
Riboulet-Bisson E, Sturme MH, Jeffery IB Effect of Lactobacillus salivarius bacteriocin Abp118 on the mouse and pig intestinal microbiota. PLoS ONE, 2012-02-17;7(2):e31113. 2012-02-17 [PMID: 22363561] (Porcine) Porcine
Show All 18 Publications.

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Product General Protocols

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By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified...  Read full blog post.

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Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Bioinformatics

Gene Symbol IL10