LANA2 Antibody (CM-A807)

Images

 
Western Blot: LANA2 Antibody (CM-A807) [NB200-167] - Detection of LANA2 in infected lysates (30 ug) using 2 ug/ml of NB 200-167. ECL detection 1.5 minutes. Lane 1: BCBL-1 (KSHV+) Lane 2: BC-1 (KSHV+/EBV+) Lane 3: BCP-1 ...read more

Product Details

Summary
Reactivity Hu, Mu, ViSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
CM-A807
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2.0 mg/ml

LANA2 Antibody (CM-A807) Summary

Immunogen
Recombinant LANA2 fusion protein.
Localization
Nuclear
Specificity
This is specific for KSHV LANA2 (residues 143-310 epitope recognition).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This LANA2 (CM-A807) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Western Blot. By Western Blot, this antibody recognizes a viral protein in KSHV infected B-cells (~66-70 kDa).
Reviewed Applications
Read 1 Review rated 5
using
NB200-167 in the following applications:

Publications
Read Publications using
NB200-167 in the following applications:

Reactivity Notes

Reacts with viral LANA2 protein and shows no cross-reactivity to cellular proteins. Also reacts with human and mouse viral KSHV proteins.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
2.0 mg/ml
Purity
Protein G purified

Alternate Names for LANA2 Antibody (CM-A807)

  • LANA 2
  • LANA-2
  • Latency-associated nuclear antigen 2
  • vIRF 3
  • vIRF3
  • vIRF-3

Background

LANA2 (latency-associated nuclear antigen 2; also known as viral interferon regulatory factor 3) is a Kaposi's sarcoma-associated herpesvirus (KSHV) latent protein which is encoded by ORFK10.5 and is exclusively expressed by virus infected B- cells. LANA2 has been shown to be critical in KSHV-mediated tumorigenesis as it inhibits p53 function, dsRNA-activated protein kinase R (PKR)-dependent apoptosis, nuclear factor kB activity, interferon regulatory factor 7-mediated interferon signal transduction and virus-mediated transcriptional activation of the alpha-interferon promoter. LANA2 stabilizes and triggers the induction of HIF-1alpha's transcriptional activity and disrupts PODs (PML oncogenic domains). Moreover, LANA2 has been shown to be required for the survival of primary effusion lymphoma (PEL) cells infected with KSHV alone or dually infected by Epstein-Barr virus (EBV) and KSHV, wherein it shows nuclear and cytoplasmic expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LANA2 Antibody (NB200-167)(7)

We have publications tested in 2 confirmed species: Human, Virus.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(5)
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Human
(4)
Virus
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Showing Publications 1 - 7 of 7.
Publications using NB200-167 Applications Species
Liang Q, Chang B, Lee P et al. Identification of the essential role of vBcl2 for KSHV lytic replication J. Virol. 2015 Mar 04 [PMID: 25740994] (WB) WB
Zuo J, Hislop AD, Leung C et al. KSHV encoded vIRF3 modulates MHC-II antigen presentation through CIITA dependent and independent mechanisms: implications for oncogenesis. J Virol 2013 Feb 28 [PMID: 23449805] (WB, Human) WB Human
Marcos-Villar L, Gallego P, Munoz-Fontela C et al. Kaposi's sarcoma-associated herpesvirus lana2 protein interacts with the pocket proteins and inhibits their sumoylation. Oncogene 2013 Jan 14 [PMID: 23318443] (WB, Human) WB Human
Marcos-Villar L, Campagna M, Lopitz-Otsoa F, Gallego P, Gonzalez-Santamaria J, Gonzalez D, Rodriguez MS, Rivas C. Covalent modification by SUMO is required for efficient disruption of PML oncogenic domains by Kaposi's sarcoma-associated herpesvirus latent protein LANA2. J Gen Virol;92(Pt 1):188-94. 2011 Jan. [PMID: 20881090] (WB, Human) WB Human
Sarek G, Jarviluoma A, Moore HM et al. Nucleophosmin phosphorylation by v-cyclin-CDK6 controls KSHV latency PLoS Pathog. 2010 Mar 19 [PMID: 20333249] (WB, Virus) WB Virus
Marcos-Villar L, Lopitz-Otsoa F, Gallego P et al. Kaposi's Sarcoma-Associated Herpesvirus Protein LANA2 Disrupts PML Oncogenic Domains Inhibits PML-Mediated Transcriptional Repression of the Survivin Gene. J Virol;83(17):8849-8858. 2009 [PMID: 19553342]
Shin, YC et al. Kaposi's sarcoma-associated herpesvirus viral IFN regulatory factor 3 stabilizes hypoxia-inducible factor-1 alpha to induce vascular endothelial growth factor expression. Cancer Res;68(6):1751-9. 2008 Mar 15. [PMID: 18339855] (ICC/IF, Human) ICC/IF Human

Review for LANA2 Antibody (NB200-167) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using NB200-167:
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WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Other 12/12/2014
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 20333249
SpeciesOther

Blocking

Blocking DetailsSee PMID 20333249

Primary Anitbody

Dilution Ratio1:1000 in PBS with 5% milk

Details

Detection NotesSee PMID 20333249

Comments

CommentsPublished in PMID 20333249.

Product General Protocols

View specific protocols for LANA2 Antibody (NB200-167): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LANA2 Antibody (NB200-167) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB200-167AF405
Alexa Fluor 488 Labeled NB200-167AF488
Alexa Fluor 647 Labeled NB200-167AF647
Alexa Fluor 700 Labeled NB200-167AF700
Biotin Labeled NB200-167B
DyLight 350 Labeled NB200-167UV
DyLight 405 Labeled NB200-167V
DyLight 488 Labeled NB200-167G
DyLight 550 Labeled NB200-167R
DyLight 650 Labeled NB200-167C
DyLight 680 Labeled NB200-167FR
DyLight 755 Labeled NB200-167IR
FITC Labeled NB200-167F
HRP Labeled NB200-167H

Additional LANA2 Products

LANA2 NB200-167

Research Areas for LANA2 Antibody (NB200-167)

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Blogs on LANA2

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Recent Reviews

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Anonymous
12/12/2014
Application: WB
Species: Other