S100B Antibody (EP1576Y)

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

S100B is a zinc- and calcium-binding protein belonging to the S100 protein family within the EF-hand (helix E-loop-helix F) subgroup (1,2). S100B plays a role in normal central nervous system development, is associated with various neurological diseases such as Alzheimer's and Parkinson's, and it serves as a marker for brain injury (1,2). The S100B protein has a homodimeric structure comprised of two 91-amino acid polypeptide monomers each with a theoretical molecular weight of 10.5 kDa (1,2). Furthermore, each monomer contains two EF-hand regions, four helixes, and a hinge region (2). S100B is predominately expressed in astrocytes, oligodendrocytes, and Schwann cells, but also other cell types including adipocytes (1,3). S100B interacts with toll-like receptor 4 (TLR4) and receptor for advanced glycation end products (RAGE), initiating downstream signaling cascades and transcription factors including JNK/JUN, NFkappaB, and p38, leading to caspase and proinflammatory cytokine production (2). Overall outcomes include neuronal apoptosis, neuroinflammation, and neurodegeneration (1,2). S100B is the most commonly studied astroglia and blood brain barrier biomarker in traumatic brain injury (TBI) (3,4). The serum levels of S100B in patients with TBI is indicative of patient outcomes, where high levels correlate with injury severity and mortality (4). S100B is often in used in combination with additional biomarkers such as glial fibrillary acidic protein (GFAP) and ubiquitin c-terminal hydrolase L1 (UCH-L1) (3,4).

References

1. Yardan, T., Erenler, A. K., Baydin, A., Aydin, K., & Cokluk, C. (2011). Usefulness of S100B protein in neurological disorders. JPMA. The Journal of the Pakistan Medical Association, 61(3), 276-281.

2. Langeh, U., & Singh, S. (2021). Targeting S100B Protein as a Surrogate Biomarker and its Role in Various Neurological Disorders. Current neuropharmacology, 19(2), 265-277. https://doi.org/10.2174/1570159X18666200729100427

3. Thelin, E. P., Nelson, D. W., & Bellander, B. M. (2017). A review of the clinical utility of serum S100B protein levels in the assessment of traumatic brain injury. Acta neurochirurgica, 159(2), 209-225. https://doi.org/10.1007/s00701-016-3046-3

4. Wang, K. K., Yang, Z., Zhu, T., Shi, Y., Rubenstein, R., Tyndall, J. A., & Manley, G. T. (2018). An update on diagnostic and prognostic biomarkers for traumatic brain injury. Expert review of molecular diagnostics, 18(2), 165-180. https://doi.org/10.1080/14737159.2018.1428089

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-Fr, WB.

Showing Publications 1 - 10 of 12. Show All 12 Publications. Collapse Publications.

Publications using NB110-57478	Applications	Species
Hughes LJ, Park R, Lee MJ et al. Yap/Taz are required for establishing the cerebellar radial glia scaffold and proper foliation Dev. Biol. 2019-10-03 [PMID: 31586559]
Nam MH, Han KS, Lee J et al. Activation of Astrocytic mu-Opioid Receptor Causes Conditioned Place Preference Cell Rep 2019-07-30 [PMID: 31365861] (IF/IHC, Mouse)	IF/IHC	Mouse
Kubo A, Misonou H, Matsuyama M et al. Distribution of endogenous normal tau in the mouse brain. J. Comp. Neurol. 2018-11-08 [PMID: 30408165] (ICC/IF, Mouse)	ICC/IF	Mouse
Okun E, Marton D, Cohen D et al. Sirt6 alters adult hippocampal neurogenesis PLoS ONE 2017-06-23 [PMID: 28644902] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Kwan J, Sczaniecka A, Heidary Arash E et al. DLG5 connects cell polarity and Hippo signaling protein networks by linking PAR-1 with MST1/2. Genes Dev. 2016-12-15 [PMID: 28087714] (IF/IHC, Mouse)	IF/IHC	Mouse
Cheng CY, Lin JG, Tang NY et al. Electroacupuncture-Like Stimulation at the Baihui (GV20) and Dazhui (GV14) Acupoints Protects Rats against Subacute-Phase Cerebral Ischemia-Reperfusion Injuries by Reducing S100B-Mediated Neurotoxicity. PLoS ONE 2014-03-14 [PMID: 24626220] (IHC-Fr, Rat)	IHC-Fr	Rat
Shimamoto S, Tsuchiya M, Yamaguchi F et al. Ca2+/S100 proteins inhibit the interactions of FKBP38 with Bcl-2 and Hsp90. Biochem J 2013-12-03 [PMID: 24295050] (WB, Human)	WB	Human
Kitazawa M, Cheng D, Tsukamoto MR et al. Blocking IL-1 Signaling Rescues Cognition, Attenuates Tau Pathology, and Restores Neuronal [beta]-Catenin Pathway Function in an Alzheimer’s Disease Model. Journal of Immunology (Baltimore, Md : 1950). 2011-11-16 [PMID: 22095718]
Liu C-H, Lin Y-W, Tang N-Y et al. Neuroprotective Effect of Uncaria rhynchophylla in Kainic Acid-Induced Epileptic Seizures by Modulating Hippocampal Mossy Fiber Sprouting, Neuron Survival, Astrocyte Proliferation, and S100B Expression. Evid Based Complement Alternat Med. 2012-01-01 [PMID: 21837247]
Furusato E, Cameron JD, Newsom RW et al. Ocular perivascular epithelioid cell tumor: report of 2 cases with distinct clinical presentations. Hum Pathol 2010-05-01 [PMID: 20236689]
Yang L, Li F, Ge W et al. Protective effects of naloxone in two-hit seizure model. Epilepsia 2010-03-01 [PMID: 19674055] (WB)	WB
Yang L, Li F, Zhang H et al. Astrocyte activation and memory impairment in the repetitive febrile seizures model. Epilepsy Res 2009-10-01 [PMID: 19643577] (WB, IF/IHC)	WB, IF/IHC
Show All 12 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	IHC-Fr	Rat	09/29/2015	View Summary Application Immunohistochemistry-Frozen Sample Tested Rat median nerve Species Rat
Enlarge	4 reviewed by: Verified Customer	IF	Mouse	09/04/2015	View Summary Application Immunofluorescence Sample Tested Mouse Ear Skin (frozen section) Species Mouse

Array NB110-57478

• S100B Antibodies
• S100B Antibody Pair
• S100B ELISA Kit
• S100B Lysate
• S100B Proteins
• S100B Proteins and Enzyme
• S100B ELISA