ATM Antibody (Y170)

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

ATM (ataxia telangiectasia mutated kinase) is the master regulator of the DNA double-strand break (DSB) repair pathway. This ubiquitously expressed serine/threonine protein kinase belongs to the PI3K-like family of proteins and responds to DSBs caused by oxidative and other genotoxic stresses (1). In addition to regulating the DNA damage response, ATM participates in vesicle and protein transport, T-cell development, gonads/neurological function, pre-B cell allelic exclusion, cell cycle control, and acts as a tumor suppressor (2,3). Defects in ATM are associated with ataxia telangiectasia (AT), T-cell acute lymphoblastic leukemia (TALL), T-prolymphocytic leukemia (TPLL), and B-cell non-Hodgkin lymphomas (BNHL) including mantle cell lymphoma (MCL) and B-cell chronic lymphocytic leukemia (BCLL) (4).

The theoretical molecular weight of ATM is 350 kDa and it has 3 main domains: a FAT (focal adhesion targeting) domain (aa 1960-2566), a PI-3/PI-4 kinase catalytic domain (aa 2712-2962), and a C-terminal FAT domain (aa 3024-3056). ATM exists as a dimer or tetramer in its inactive state. Upon sensing DNA damage, the MRE11-RAD50-NBS1 (MRN) complex recruits ATM. The intricate process of ATM activation involves acetylation by KAT5/TIP60, autophosphorylation at Ser-1981, and dissociation into catalytically active monomers (5). Following activation, ATM phosphorylates multiple substrates such as p53/TP53 and Chk2 involved in DNA repair, checkpoint signaling, and the apoptosis pathway.

References

1. Paull TT. (2015) Mechanisms of ATM Activation. Annu Rev Biochem. 84:711-38. PMID: 25580527

2. Chaudhary MW and Al-Baradie RS. (2014) Ataxia-telangiectasia: future prospects. Appl Clin Genet. 7:159-167. PMID: 25258552

3. Stagni V, Cirotti C, and Barila D. (2018) Ataxia-Telangiectasia Mutated Kinase in the Control of Oxidative Stress, Mitochondria, and Autophagy in Cancer: A Maestro With a Large Orchestra. Front Oncol. 8:73. PMID: 29616191

4. Gumy-Pause F, Wacker P, and Sappino AP. (2004) ATM gene and lymphoid malignancies. Leukemia. 18(2):238-42. PMID: 14628072

5. Adamowicz M. (2018) Breaking up with ATM. J Immunol Sci. 2(1):26-31. PMID: 29652413

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.

Showing Publications 1 - 10 of 15. Show All 15 Publications. Collapse Publications.

Publications using NB110-55475	Applications	Species
Fievet A, Bellanger D, Zahed L et al. DNA repair functional analyses of NBN hypomorphic variants associated with NBN-related infertility Hum Mutat. 2019-11-15 [PMID: 31729086] (WB, Human)	WB	Human
Kim D, Mecham RP, Nguyen NH, Roy S Functional classification of ATM variants in ataxia-telangiectasia patients Hum. Mutat. 2019-05-03 [PMID: 31050087] (WB)	WB
Wallace HA, Rana V, Nguyen HQ, Bosco G Three new cases of ataxia-telangiectasia-like disorder: No impairment of the ATM pathway, but S-phase checkpoint defect Hum. Mutat. 2019-04-29 [PMID: 31033087] (WB)	WB
You J, Sobreira NL, Gable DL et al. A Syndromic Intellectual Disability Disorder Caused by Variants in TELO2, a Gene Encoding a Component of the TTT Complex. Am. J. Hum. Genet. 2016-05-05 [PMID: 27132593] (WB, Human)	WB	Human
Qi Y, Schoene NW, Lartey FM et al. Selenium compounds activate ATM-dependent DNA damage response via the mismatch repair protein hMLH1 in colorectal cancer cells. J Biol Chem 2010-10-01 [PMID: 20709753] (WB)	WB
Boichuk S, Hu L, Hein J, Gjoerup OV. Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen. J Virol 2010-08-01 [PMID: 20519379] (WB)	WB
Wu M, Kang MM, Schoene NW et al. Selenium compounds activate early barriers of tumorigenesis. J Biol Chem 2010-04-01 [PMID: 20157118] (WB)	WB
Togano T, Sasaki M, Watanabe M et al. Induction of oncogene addiction shift to NF-kappaB by camptothecin in solid tumor cells. Biochem Biophys Res Commun 2009-12-01 [PMID: 19778522] (WB)	WB
Schwartz RA, Carson CT, Schuberth C et al. Adeno-associated virus replication induces a DNA damage response coordinated by DNA-dependent protein kinase. J Virol 2009-06-01 [PMID: 19339345] (WB)	WB
Cheng WH, Muftic D, Muftuoglu M et al. WRN is required for ATM activation and the S-phase checkpoint in response to interstrand cross-link-induced DNA double-strand breaks. Mol Biol Cell 2008-09-01 [PMID: 18596239] (WB)	WB
Lakdawala SS, Schwartz RA, Ferenchak K et al. Differential requirements of the C terminus of Nbs1 in suppressing adenovirus DNA replication and promoting concatemer formation. J Virol 2008-09-01 [PMID: 18562516] (WB)	WB
Li H, Baskaran R, Krisky DM et al. Chk2 is required for HSV-1 ICP0-mediated G2/M arrest and enhancement of virus growth. Virology 2008-05-01 [PMID: 18321553] (WB)	WB
Gorodetsky E, Calkins S, Ahn J, Brooks PJ ATM, the Mre11/Rad50/Nbs1 complex, and topoisomerase I are concentrated in the nucleus of Purkinje neurons in the juvenile human brain. DNA Repair (Amst) 2007-11-01 [PMID: 17706468]
Biton S, Gropp M, Itsykson P et al. ATM-mediated response to DNA double strand breaks in human neurons derived from stem cells. DNA Repair (Amst) 2007-01-01 [PMID: 17178256] (ICC/IF)	ICC/IF
Biton S, Dar I, Mittelman L et al. Nuclear ataxia-telangiectasia mutated (ATM) mediates the cellular response to DNA double strand breaks in human neuron-like cells. J Biol Chem 2006-06-01 [PMID: 16627474] (ICC/IF)	ICC/IF
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Array NB110-55475

• ATM Antibodies
• ATM ELISA Kits
• ATM Proteins