| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-598SS | |||
| Azide and BSA Free | NBP2-80600 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-600SS | |||
| Azide and BSA Free | NBP2-80602 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36429-0.025mg | |||
| Azide and BSA Free | NBP2-81009 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43262-0.5mg | |||
| BSA Free | NBP1-43262-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-113SS | |||
| Azide and BSA Free | NBP2-80853 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89065-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50057SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-213SS | |||
![Western Blot: BRCA1 Antibody (RAY) [NB100-598] - Analysis of BRCA1 on MCF7 lysate.](http://images.novusbio.com/fullsize/BRCA1-Antibody-RAY-Western-Blot-NB100-598-img0006.jpg "Western Blot: BRCA1 Antibody (RAY) [NB100-598] - Analysis of BRCA1 on MCF7 lysate.")
![Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (RAY) [NB100-598] - BRCA (RAY) antibody was tested in MCF-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/BRCA1-Antibody-RAY-Immunocytochemistry-Immunofluorescence-NB100-598-img0005.jpg)
![Flow Cytometry: BRCA1 Antibody (RAY) [NB100-598] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using BRCA1 antibody (dark blue) and isotype control (orange). An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/BRCA1-Antibody-RAY-Flow-Cytometry-NB100-598-img0008.jpg)
![Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 using NB100-600.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Western-Blot-NB100-600-img0004.jpg "Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 using NB100-600.")
![Flow Cytometry: BRCA1 Antibody (MU) [NB100-600] - An intracellular stain was performed on MCF7 cells with BRCA1 Antibody [MU] NB100-600C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Flow-Cytometry-NB100-600-img0006.jpg)
![Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 in MCF-7 whole cell lysate using NB100-600. 2 minute ECL exposure.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Western-Blot-NB100-600-img0003.jpg)
![Flow Cytometry: BRCA1 Antibody (MU) [NB100-600] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using BRCA1 antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells was used.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Flow-Cytometry-NB100-600-img0005.jpg)
![Western Blot: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of VEGF R2/KDR/Flk-1 (clone 2C6) in partial recombinant protein.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Western-Blot-NBP2-36429-img0018.jpg "Western Blot: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of VEGF R2/KDR/Flk-1 (clone 2C6) in partial recombinant protein.")
![Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Antibody (1:50) was tested in HUVEC cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunocytochemistry-Immunofluorescence-NBP2-36429-img0012.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of a malignant stromal tumor of the human small bowel with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0017.jpg)
![Flow (Cell Surface): VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - HepG2 cells were stained (surface) with antibody (orange) or isotype control, mouse IgG1 (blue). Positive staining observed using FITC conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Flow-Cell-Surface-NBP2-36429-img0011.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of human normal kidney with antibody at 5 ug/ml concentration. The cuboidal epithelial cells of of various tubules/ducts showed a specific cytoplasmic immunopositivity of VEGF R2/KDR/Flk-1 protein.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0013.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of Human SCC of Esophagus with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm while the tumor stroma was negative for VEGF R2/KDR/Flk-1 immunopositivity.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0014.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of Human Hepatocellular Carcinoma with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0015.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Aanalysis of FFPE transverse section of normal human skeletal muscle with antibody at 5 ug/ml concentration. The myocytes showed a moderate diffused to granular staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0016.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-31330_rabbit-polyclonal-cyclin-a2-antibody-immunocytochemistry-immunofluorescence-141120251833319.jpg "Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.")
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Using the Allophycocyanin direct conjugate A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0020.jpg "Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Using the Allophycocyanin direct conjugate A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.")
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Analysis using the Biotin conjugate of NBP1-43262. Staining of C57Bl/6 splenocytes with 0.125 ug of Rat IgG2a Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Mouse (B7-H1) Biotin (filled histogram) followed by Streptavidin PE.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0019.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Staining of mouse splenocytes with Anti-Mouse B7-H1/PD-L1/CD274) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0021.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in human primary colon cancer cell using anti-PD-L1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0011.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in HT29 cells using anti-PD-L1 antibody. The data shows the detection of total PD-L1 status in the cancer cells. Image from verified customer review.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0010.jpg)
![Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Recombinant human MMP-2 expressed from CHO cells was was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MMP-2 in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Western-Blot-NB200-113-img0007.jpg "Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Recombinant human MMP-2 expressed from CHO cells was was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MMP-2 in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Samples: 1) Human Recombinant MMP-2, and 2)Schwann Cell Culture Medium Run on 10% SDS-PAGE, followed by electroblot to nitrocellulose and slot blot immunolabeling. Antibodies: primary - NB200-113, secondary - anti-mouse-biotin. Detection: avidin-HRP. Chromagen: DAB/H202.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Western-Blot-NB200-113-img0009.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Analysis of a FFPE human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). MMP2 immunoreactivity was observed in the cytoplasm of the cancer cells and the stromal cells, and in the inter-cellular spaces (secreted MMP2). The cancer cells present in the core of tumor areas were found to be negative for MMP2 staining.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Immunohistochemistry-Paraffin-NB200-113-img0006.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Analysis of a FFPE human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This MMP2 antibody generated an expected immunoreactivity of MMP2 protein in the cytoplasm of most of the cancer cells and the stromal cells, and in the inter-cellular spaces (secreted pool of MMP2). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Immunohistochemistry-Paraffin-NB200-113-img0003.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Upper image: MMP-2 FITC (green), DAPI (blue)Lower image: phase contrast of human choroid plexus (Alzheimer's disease). Image from verified customer review.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Immunohistochemistry-Paraffin-NB200-113-img0011.jpg)
![Flow Cytometry: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Flow Cytometry: MMP-2 Antibody (2C1) [Alexa Fluor® 405] [NB200-113AF405] - CD4+ T cells were activated for 24h before being fixed and permeabilized to detect MMP-2. Image using the Alexa Fluor 405 form of this antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Flow-Cytometry-NB200-113-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP1-89065] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89065-img0011.jpg "Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP1-89065] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: c-Fos Antibody [NBP1-89065] - Staining of human stomach shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NBP1-89065-img0019.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody [NBP1-89065] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NBP1-89065-img0016.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody [NBP1-89065] - Staining of human kidney shows weak to moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NBP1-89065-img0017.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody [NBP1-89065] - Staining of human pancreas shows very weak and few nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NBP1-89065-img0018.jpg)
![Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Whole cell extracts (30 and 50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Western-Blot-NB100-404-img0003.jpg "Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Whole cell extracts (30 and 50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody at a dilution of 1:500.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (6B4) [NB100-404] - Staining of BRCA1 nuclear foci induced by ionizing radiation. IR-treated (2 hr /4 gray IR) U2OS cells were pre-extracted with CSK buffer on ice for 4 min before fixation with 4% PFA in room temperature, and then subjected to immunostaining. DAPI was used to counterstain nucleus. 6B4 was used. Secondary antibody (Alexa Fluor-488) used for detection of primary antibody (BRCA1 antibody 6B4)](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Immunocytochemistry-Immunofluorescence-NB100-404-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade diluted at 1:500.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Western-Blot-NB100-404-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - NB100-404 was used at 1:1000 dilution for Western blot assay of lysates from cells transfected with control or BRCA1-specific siRNA. Lysates were prepared at the indicated times following transfection. RAD50 antibody (13B3) (NB100-147) was used as a loading control.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Western-Blot-NB100-404-img0004.jpg)
![Immunoprecipitation: BRCA1 Antibody (6B4) [NB100-404] - 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Immunoprecipitation-NB100-404-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - DAG-induced DNA damage is repaired by homologous recombination. A549 cells were transfected with either negative control (C) or three BRCA1-targeting siRNAs (B1, siBRCA1-2; B2, siBRCA1-15; or B3, siBRCA1-17) for 24 h. Cells were then seeded in 96-well culture plates and treated with different concentrations of VAL-083 (0, 100 nM, 500 nM, 1 uM, 1.5 uM, 2.5 uM, 5 uM, 10 uM, 25 uM, 50 uM, 100 uM, and 200 uM) for 5 days. Following the treatment, crystal violet assay was performed to detect the absorbance at 560 nm wavelength. Cell lysates from the control or BRCA1-knockdown cells were analyzed by western blot with antibody against BRCA1, and GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-018-1069-9) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Knockdown-Validated-NB100-404-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody, diluted at 1:500. A HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Knockout-Validated-NB100-404-img0015.jpg)
![Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (NB100-404) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/nb100-404_mouse-monoclonal-brca1-antibody-6b4-91020242091412.jpg)
![Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20535-img0006.jpg "Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535] - Human breast carcinoma. SOD2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP2-20535-img0014.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-20535_rabbit-polyclonal-sod2-mn-sod-antibody-1610202419405789.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody (2G2) [NBP2-37492] - Analysis of colon cancer tissues using FOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Immunohistochemistry-NBP2-37492-img0005.jpg "Immunohistochemistry-Paraffin: c-Fos Antibody (2G2) [NBP2-37492] - Analysis of colon cancer tissues using FOS mouse mAb with DAB staining.")
![Western Blot: c-Fos Antibody (2G2) [NBP2-37492] - Western blot analysis using FOS mouse mAb against HeLa (1), and HeLa (2) cell lysate.](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Western-Blot-NBP2-37492-img0003.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody (2G2) [NBP2-37492] - Analysis of cerebellum tissues using FOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Immunohistochemistry-NBP2-37492-img0006.jpg)
![Flow Cytometry: c-Fos Antibody (2G2) [NBP2-37492] - Flow cytometric analysis of HeLa cells using FOS mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Flow-Cytometry-NBP2-37492-img0004.jpg)
![Western Blot: c-Fos Antibody (2G2) [NBP2-37492] - Western blot analysis using FOS mAb against human FOS (AA: 116-298) recombinant protein. (Expected MW is 45.8 kDa)](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Western-Blot-NBP2-37492-img0002.jpg)
![ELISA: c-Fos Antibody (2G2) [NBP2-37492] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-ELISA-NBP2-37492-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP2-50057] - Analysis of HeLa cells stained with rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000 in red, and mouse mAb to tubulin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells. Mouse hippocampus (C) or olfactory bulb sections (D) stained with NBP2-50057, dilution 1:20,000 in red, and mouse mAb to NF-L, dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NBP2-50057-img0001.jpg "Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP2-50057] - Analysis of HeLa cells stained with rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000 in red, and mouse mAb to tubulin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells. Mouse hippocampus (C) or olfactory bulb sections (D) stained with NBP2-50057, dilution 1:20,000 in red, and mouse mAb to NF-L, dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.")
![Western Blot: c-Fos Antibody [NBP2-50057] - Analysis of cell lysates using rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000, in green, and mouse mAb to GAPDH, dilution 1:5,000, in red, used as a loading control. [1] protein standard (red), [2] HeLa cells grown in FBS free media, [3] HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours, [4] rat cortical neurons, [5] rat cortical neurons treated with membrane depolarization buffer for 5 hours. Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NBP2-50057-img0002.jpg)
![Western Blot: Smac Antibody (78-1-118) [NB500-213] - HeLa whole cell extract lysates.](http://images.novusbio.com/fullsize/Smac-Antibody-78-1-118-Western-Blot-NB500-213-img0002.jpg "Western Blot: Smac Antibody (78-1-118) [NB500-213] - HeLa whole cell extract lysates.")