Galectin-3 Antibody (A3A12) [DyLight 488]

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Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-Fr, IHC-P
Clone
A3A12
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

Galectin-3 Antibody (A3A12) [DyLight 488] Summary

Immunogen
Full length recombinant human galectin-3.
Epitope
The epitope for this antibody has been mapped to the first 58 amino acids of Galectin 3.
Specificity
Galectin 3 (A3A12)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LGALS3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB300-538G in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 19744479). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Galectin-3 Antibody (A3A12) [DyLight 488]

  • AGE-R3
  • Carbohydrate-binding protein 35
  • CBP 35,35 kDa lectin
  • CBP35
  • GAL3
  • Gal-3
  • galactin-3
  • Galactose-specific lectin 3
  • Galactoside-binding protein
  • GALBPCBP35
  • Galectin3
  • Galectin-3
  • GALIG
  • IgE-binding protein
  • L29
  • L31
  • L-31
  • Laminin-binding protein
  • Lectin L-29
  • lectin, galactoside-binding, soluble, 3
  • LGALS2
  • LGALS3
  • Mac-2 antigen
  • Mac-2
  • MAC2GAL3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Galectin-3 Antibody (NB300-538G) (0)

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Review for Galectin-3 Antibody (NB300-538G) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB300-538G:
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Images Ratings Applications Species Date Details
 Galectin-3 NB300-538G
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reviewed by:
Anonymous
Human 08/31/2017
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Summary

Sample TestedT98G human glioblastoma cell line
SpeciesHuman
LotRL242392-04271

Comments

CommentsT98G cells were treated with LLOMe (1 uM) for 1 h to induce lysosomal damage. Cells were fixed (4% paraformaldehyde) 6 h after washout, blocked with 10% goat serum in 0.1% PBST (1 h, RT), stained with DyLight 488 conjugated galectin-3 antibody (green) (overnight, 4C).The following day, samples were washed with cold PBST, mounted using Prolong Gold mountant with DAPI (blue).

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Galectin-3 Antibody (NB300-538G) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 Labeled NB300-538UV
DyLight 405 Labeled NB300-538V
DyLight 680 Labeled NB300-538FR
DyLight 755 Labeled NB300-538IR

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Recent Reviews

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5
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2
0
1
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Anonymous
08/31/2017
Application:
Species: Human

Bioinformatics

Gene Symbol LGALS3
Entrez