Immunocytochemistry: Galectin-3 Antibody (A3A12) [DyLight 488] [NB300-538G] - LLOMe treatment results in lysosomal damage in T98G glioblastoma cells as evidenced by the accumulation of galectin-3 positive vesicles. T98G ...read more
26 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NB300-538G in the following applications:
Rabbit reactivity reported in scientific literature (PMID: 19744479). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Protein A or G purified
Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for Galectin-3 Antibody (A3A12) [DyLight 488]
Carbohydrate-binding protein 35
CBP 35,35 kDa lectin
Galactose-specific lectin 3
lectin, galactoside-binding, soluble, 3
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
T98G cells were treated with LLOMe (1 uM) for 1 h to induce lysosomal damage. Cells were fixed (4% paraformaldehyde) 6 h after washout, blocked with 10% goat serum in 0.1% PBST (1 h, RT), stained with DyLight 488 conjugated galectin-3 antibody (green) (overnight, 4C).The following day, samples were washed with cold PBST, mounted using Prolong Gold mountant with DAPI (blue).
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