WRN Antibody

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Independent Antibodies: Western Blot: WRN Antibody [NB100-472] - Detection of human WRN on HeLa whole cell lysate using NB100-472. WRN was immunoprecipitated with NB100-472 or rabbit anti-WRN antibody NB100-471.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

WRN Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1400 and the C-terminus (residue 1432) of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191 (GeneID 7486).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WRN
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:25000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockout Validated
Theoretical MW
162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
WRN Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-472 in the following applications:

Publications
Read Publications using
NB100-472 in the following applications:

  • KO
    1 publication
  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30971823).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for WRN Antibody

  • DKFZp686C2056
  • DNA helicase, RecQ-like type 3
  • EC 3.1
  • EC 3.6.1
  • EC 3.6.4.12
  • Exonuclease WRN
  • RecQ protein-like 2
  • RecQ3
  • RECQ3RECQL3
  • RECQL2Werner syndrome ATP-dependent helicase
  • Werner syndrome
  • Werner syndrome, RecQ helicase-like

Background

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner Syndrome, an autosomal recessive disorder characterized by premature aging.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for WRN Antibody (NB100-472)(6)

Review for WRN Antibody (NB100-472) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Canine and Feline.

Reviews using NB100-472:
Filter by Applications
WB
(1)
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Filter by Species
Canine and Feline
(1)
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Images Ratings Applications Species Date Details
Western Blot WRN NB100-472
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reviewed by:
Anonymous
WB Canine and Feline 06/29/2017
View

Summary

ApplicationWestern Blot
Sample TestedPancreas tissue,Stomach tissue,Skin tissue,Testis tissue
SpeciesCanine and Feline
LotA2

Comments

CommentsThis antibody will be used for IHC-P in both canine and feline tissue (results not yet available).

Product General Protocols

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Video Protocols

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FAQs for WRN Antibody (NB100-472) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional WRN Products

Array NB100-472

Bioinformatics Tool for WRN Antibody (NB100-472)

Discover related pathways, diseases and genes to WRN Antibody (NB100-472). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for WRN Antibody (NB100-472)

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Pathways for WRN Antibody (NB100-472)

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PTMs for WRN Antibody (NB100-472)

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Research Areas for WRN Antibody (NB100-472)

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Blogs on WRN.

Not as Pluripotent as You Used to Be: Embryonic Stem Cell Markers and the Aging Process
We at Novus Biologicals recently extended our antibody catalog to include several embryonic stem cells (ESC) antibodies validated for use in FACS (fluorescent activated cell sorting) assays. Among them was Oct4, which recently became the focus of an i...  Read full blog post.

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Recent Reviews

4
5
0
4
1
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0
2
0
1
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Anonymous
06/29/2017
Application: WB
Species: Canine and Feline

Bioinformatics

Gene Symbol WRN
Entrez
Uniprot