c-Fos Antibody

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Western Blot: c-Fos Antibody [NB110-75039] - Analysis on marmoset brain lysate prepared in Ripa buffer containing 1% Triton-X100; 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution ...read more
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody [NB110-75039] - Rat trigeminal using Rabbit antibody to cFos: whole serum at a 1:500 dilution.
Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to cFos (c-fos): whole serum at 1: 300 dilution incubated overnight at 4C and developed with ...read more
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody [NB110-75039] - Rabbit antibody to cFos (c-fos): whole serum (NB110-75039) at a 1 : 500 dilution and DAPI counterstained appearing in blue.
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody [NB110-75039] - Rabbit antibody to cFos (c-fos): whole serum (NB110-75039) at a 1 : 500 dilution and DAPI counterstained appearing in blue.
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody [NB110-75039] - Rat trigeminal using Rabbit antibody to cFos: whole serum at a 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody [NB110-75039] - Rat olfactory bulb using Rabbit antibody to cFos: whole serum at a 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody [NB110-75039] - Rat olfactory bulb using Rabbit antibody to cFos: whole serum at a 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody [NB110-75039] - Rat trigeminal using Rabbit antibody to cFos: whole serum at a 1:500 dilution.
Immunohistochemistry: c-Fos Antibody [NB110-75039] - IHC on rat brain (free floating sections) using Rabbit antibody to cFos (c-fos): whole serum at 1: 300 dilution incubated overnight at 4C and developed with ...read more
Immunohistochemistry: c-Fos Antibody [NB110-75039] - IHC on rat brain (free floating sections) using Rabbit antibody to cFos (c-fos): whole serum at 1: 300 dilution incubated overnight at 4C and developed with ...read more
Immunohistochemistry: c-Fos Antibody [NB110-75039] - IHC on rat brain (free floating sections) using Rabbit antibody to cFos (c-fos): whole serum at 1: 300 dilution incubated overnight at 4C and developed with ...read more
Immunohistochemistry: c-Fos Antibody [NB110-75039] - IHC on rat brain (free floating sections) using Rabbit antibody to cFos (c-fos): whole serum at 1: 300 dilution incubated overnight at 4C and developed with ...read more
Immunohistochemistry: c-Fos Antibody [NB110-75039] - IHC on rat brain (free floating sections) using Rabbit antibody to cFos (c-fos): whole serum at 1: 300 dilution incubated overnight at 4C and developed with ...read more
Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC on rat brain (paraffin section) using Rabbit antibody to cFos (c-fos): whole serum at 1: 300 dilution incubated overnight at 4C and developed with ...read more
Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC on rat brain (paraffin section) using Rabbit antibody to cFos (c-fos): whole serum at 1: 300 dilution incubated overnight at 4C and developed with ...read more
Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC on rat brain (paraffin section) using Rabbit antibody to cFos (c-fos): whole serum at 1: 300 dilution incubated overnight at 4C and developed with ...read more
Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC on rat brain (paraffin section) using Rabbit antibody to cFos (c-fos): whole serum at 1: 300 dilution incubated overnight at 4C and developed with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

c-Fos Antibody Summary

Immunogen
A synthetic peptide from aa region 300-380 of human cFos protein conjugated to an immunogenic carrier protein has been used as the antigen. The peptide is homologous with the corresponding sequence derived from cFos protein in many other species including rat, mouse, hamster and cat.
Localization
Nucleus.
Specificity
cFOS.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOS
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 : 1000 - 1 : 2000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:400-1:2000
Application Notes
Immunohistochemistry-Paraffin/Frozen on free floating sections, Immunofluorescence and Western Blot
Positive Control
c-Fos Lysate (NBL1-10794)

Reactivity Notes

Confirmed reactivity in Marmoset.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified
Reconstitution Instructions
Please note the larger size comes in lyophilized form and the sample size will be in liquid formate. Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for c-Fos Antibody

  • activator protein 1
  • AP-1
  • cellular oncogene c-fos
  • Cellular oncogene fos
  • cFos
  • c-Fos
  • FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)
  • FBJ murine osteosarcoma viral oncogene homolog
  • FOS
  • G0/G1 switch regulatory protein 7
  • G0S7
  • proto-oncogene c-Fos
  • v-fos FBJ murine osteosarcoma viral oncogene homolog

Background

FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB; INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily; SIMILARITY: Contains 1 bZIP domain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for c-Fos Antibody (NB110-75039). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for some c-fos antibodies that could work in IHC.
    • Here are a list of products which might be of interest to you: Product List. We have additional filters on the left which may help you narrow down based on host species, target species, clonality, etc. We will guarantee these to work for all stated species and applications listed on the datasheet.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for c-Fos Antibody (NB110-75039)

Discover related pathways, diseases and genes to c-Fos Antibody (NB110-75039). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for c-Fos Antibody (NB110-75039)

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Blogs on c-Fos.

The C99 fragment of amyloid precursor protein (APP)
Alzheimer’s Disease (AD) is a neurodegenerative disorder that is characterized by an abundance of the beta-amyloid peptide in the brain.  When AD was first discovered, it was determined that beta-amyloid was produced as a result of the prote...  Read full blog post.

Estrogen Related Receptors Play Roles in Cancer and Neurodegeneration
By Eric NeeleyEstrogen receptors come in the form of two distinct forms, ER alpha and ER beta. These nuclear receptors are predominantly activated by the hormone 17-beta-estradiol to control transcription of genes throughout the immune, nervous, car...  Read full blog post.

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Bioinformatics

Gene Symbol FOS
Entrez
Uniprot