Galectin-3 Antibody (A3A12)

Images

 
Western Blot: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of 25 ug of MCF-7 (Lane 1), Hela (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.
Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - LLOMe treatment results in lysosomal damage in T98G glioblastoma cells as evidenced by the accumulation of galectin-3 positive vesicles. ...read more
Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Human colon tissue (right) compared with a negative control in the absence of ...read more
Flow Cytometry: Galectin-3 Antibody (A3A12) [NB300-538] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HT-29 cells with Galectin-3 (A3A12) antibody NB300-538AF647 (blue) and a ...read more
Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells.
Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of rat olfactory bulb
Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of MCF-7 cells.
Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of HeLa cells.
Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Mouse colon tissue (right) compared with a negative control in the absence of ...read more
Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Human ovarian carcinoma (right) compared with a negative control in the absence ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
A3A12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Galectin-3 Antibody (A3A12) Summary

Immunogen
Full length recombinant human galectin-3.
Epitope
The epitope for this antibody has been mapped to the first 58 amino acids of Galectin 3.
Specificity
Galectin 3 (A3A12)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LGALS3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 6 ug/ml
  • Immunohistochemistry-Paraffin 6 ug/ml
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Galectin-3 Lysate (NBP2-64748)
Publications
Read Publications using
NB300-538 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 19744479). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Galectin-3 Antibody (A3A12)

  • AGE-R3
  • Carbohydrate-binding protein 35
  • CBP 35,35 kDa lectin
  • CBP35
  • GAL3
  • Gal-3
  • galactin-3
  • Galactose-specific lectin 3
  • Galactoside-binding protein
  • GALBPCBP35
  • Galectin3
  • Galectin-3
  • GALIG
  • IgE-binding protein
  • L29
  • L31
  • L-31
  • Laminin-binding protein
  • Lectin L-29
  • lectin, galactoside-binding, soluble, 3
  • LGALS2
  • LGALS3
  • Mac-2 antigen
  • Mac-2
  • MAC2GAL3

Background

This antibody has been shown to potentiate the binding of Galectin 3 to IgG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Galectin-3 Antibody (NB300-538)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
All Applications
Filter By Species
Mouse
(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Galectin-3 Antibody (NB300-538) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-538AF405
Alexa Fluor 488 Labeled NB300-538AF488
Alexa Fluor 647 Labeled NB300-538AF647
Alexa Fluor 700 Labeled NB300-538AF700
DyLight 350 Labeled NB300-538UV
DyLight 405 Labeled NB300-538V
DyLight 488 Labeled NB300-538G
DyLight 680 Labeled NB300-538FR
DyLight 755 Labeled NB300-538IR

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Bioinformatics

Gene Symbol LGALS3
Entrez
Uniprot