Ctip1 Antibody

Western Blot: Ctip1 Antibody [NB600-261] - Detection of Mouse Bcl11a by Western Blot. Samples: Whole cell lysate (50 ug) from L1210, TCMK-1, and 4T1 cells. Antibodies: Affinity purified rabbit anti-Bcl11a antibody NB600-261 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: Ctip1 Antibody [NB600-261] - Detection of Human Bcl11a on HeLa whole cell lysate using NB600-261. Bcl11a was also immunoprecipitated using affinity purified rabbit anti-Bcl11a antibodies NB600-258 and NB600-259.

• Reactivity: Hu, Mu
• Applications: WB, ChIP, Flow, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Ctip1 Antibody Summary

• Immunogen: A synthetic peptide which maps to a region between residues 775 and the C-terminus (residue 835) of human B-cell CLL/Lymphoma 11A using the numbering given in Swiss-Prot entry Q9H165 (GeneID 53335).
• Specificity: NB600-261 is specific for human Bcl-11a protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL11A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:5000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 2 - 10 ug/mg lysate
• Application Notes: NB600-261 has been successfully used for Flow Cytometry as reported by a customer review. Use in Chromatin Immunoprecipitation was reported in a scientific publication (PMID: 23741395).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ctip1 Antibody

• B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2
• B-cell CLL/lymphoma 11A (zinc finger protein)
• B-cell CLL/lymphoma 11A
• B-cell lymphoma/leukemia 11A
• BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1
• BCL-11A
• BCL11A-S
• BCL11A-XL
• C2H2-type zinc finger protein
• COUP-TF-interacting protein 1
• CTIP1ecotropic viral integration site 9
• ecotropic viral integration site 9 homolog
• Ecotropic viral integration site 9 protein homolog
• EVI-9
• EVI9FLJ34997
• HBFQTL5
• KIAA1809BCL11A-L
• Zinc finger protein 856
• ZNF856FLJ10173

Background

The B cell leukemia 11A protein (BCL11A/Evi9/CTIP1) has been implicated in hematopoietic cell development and malignancies. BCL11A is a transcriptional repressor that binds directly to a GC-rich motif and is also recruited to a promoter template via interaction with the orphan nuclear receptor, chicken ovalbumin upstream promoter transcription factor II. In both cases, BCL11A-mediated transcriptional repression is only minimally reversed by trichostatin A, suggesting the possible lack of involvement of class I or II histone deacetylases. Nonetheless, chromatin immunoprecipitation assays revealed that expression of BCL11A in mammalian cells resulted in deacetylation of histones H3 and/or H4 that were associated with the promoter region of a reporter gene. BCL11A mediated transcriptional repression, as well as deacetylation of histone H3/H4 in BCL11A-transfected cells, was partially reversed by nicotinamide, an inhibitor of class III histone deacetylases such as SIRT1. SIRT1 was found to interact directly with BCL11A and was recruited to the promoter template in a BCL11A-dependent manner leading to transcriptional repression. These findings indicate a role for SIRT1 in transcriptional repression mediated by BCL11A in mammalian cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ctip1 Antibody (NB600-261)(4)

Publications using NB600-261

• Martyn GE, Wienert B, Yang L et al. Natural regulatory mutations elevate the fetal globin gene via disruption of BCL11A or ZBTB7A binding. Nat. Genet. Apr 1 2018 [PMID: 29610478] (WB)
• Zhu X, Hu T, Ho MH et al. Hydroxyurea differentially modulates activator and repressors of g-globin gene in erythroblasts of responsive and non-responsive patients with sickle cell disease in correlation with Index of Hydroxyurea Responsiveness. Haematologica. 2017 Sep 29 [PMID: 28971909] (Human)
• Wu X, Satpathy AT, Kc W et al. Bcl11a Controls Flt3 Expression in Early Hematopoietic Progenitors and Is Required for pDC Development In Vivo. PLoS One 2013 May 31 [PMID: 23741395] (ChIP, Mouse)
• J Xu, V G Sankaran, M Ni et al. Transcriptional silencing of {gamma}-globin by BCL11A involves long-range interactions and cooperation with SOX6. Genes & Dev; 24 (8): 783-798. 2010 [PMID: 20395365]

Review for Ctip1 Antibody (NB600-261) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Zhiwei Zhou Flow Mouse 09/26/2012

Summary

• Application: Flow Cytometry
• Sample Tested: mouse B cell
• Species: Mouse
• Lot: A2

Blocking

• Blocking Details: 2%BSA

Primary Anitbody

• Dilution Ratio: 1ug/ml

Details

• Fixation Details: 4% PFA
• Permeabilization Details: 0.1%Triton 100
• Wash Description: BD Wash buffer

Bioinformatics

• Gene Symbol: BCL11A
• Entrez:
  • 53335(Human)
• Uniprot:
  • Q9H165(Human)