Ctip1 Antibody

Images

 
Western Blot: Ctip1 Antibody [NB600-261] - Detection of Mouse Bcl11a by Western Blot. Samples: Whole cell lysate (50 ug) from L1210, TCMK-1, and 4T1 cells. Antibodies: Affinity purified rabbit anti-Bcl11a antibody ...read more
Immunoprecipitation: Ctip1 Antibody [NB600-261] - Detection of Human Bcl11a on HeLa whole cell lysate using NB600-261. Bcl11a was also immunoprecipitated using affinity purified rabbit anti-Bcl11a antibodies NB600-258 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, Flow, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Ctip1 Antibody Summary

Immunogen
A synthetic peptide which maps to a region between residues 775 and the C-terminus (residue 835) of human B-cell CLL/Lymphoma 11A using the numbering given in Swiss-Prot entry Q9H165 (GeneID 53335).
Specificity
NB600-261 is specific for human Bcl-11a protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL11A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 2 to 10 ug/mg lysate
Application Notes
NB600-261 has been successfully used for Flow Cytometry as reported by a customer review. Use in Chromatin Immunoprecipitation was reported in a scientific publication (PMID: 23741395).
Reviewed Applications
Read 1 Review rated 4
using
NB600-261 in the following applications:

Publications
Read Publications using
NB600-261 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ctip1 Antibody

  • B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2
  • B-cell CLL/lymphoma 11A (zinc finger protein)
  • B-cell CLL/lymphoma 11A
  • B-cell lymphoma/leukemia 11A
  • BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1
  • BCL-11A
  • BCL11A-S
  • BCL11A-XL
  • C2H2-type zinc finger protein
  • COUP-TF-interacting protein 1
  • CTIP1ecotropic viral integration site 9
  • ecotropic viral integration site 9 homolog
  • Ecotropic viral integration site 9 protein homolog
  • EVI-9
  • EVI9FLJ34997
  • HBFQTL5
  • KIAA1809BCL11A-L
  • Zinc finger protein 856
  • ZNF856FLJ10173

Background

The B cell leukemia 11A protein (BCL11A/Evi9/CTIP1) has been implicated in hematopoietic cell development and malignancies. BCL11A is a transcriptional repressor that binds directly to a GC-rich motif and is also recruited to a promoter template via interaction with the orphan nuclear receptor, chicken ovalbumin upstream promoter transcription factor II. In both cases, BCL11A-mediated transcriptional repression is only minimally reversed by trichostatin A, suggesting the possible lack of involvement of class I or II histone deacetylases. Nonetheless, chromatin immunoprecipitation assays revealed that expression of BCL11A in mammalian cells resulted in deacetylation of histones H3 and/or H4 that were associated with the promoter region of a reporter gene. BCL11A mediated transcriptional repression, as well as deacetylation of histone H3/H4 in BCL11A-transfected cells, was partially reversed by nicotinamide, an inhibitor of class III histone deacetylases such as SIRT1. SIRT1 was found to interact directly with BCL11A and was recruited to the promoter template in a BCL11A-dependent manner leading to transcriptional repression. These findings indicate a role for SIRT1 in transcriptional repression mediated by BCL11A in mammalian cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ChIP, Flow, IP

Publications for Ctip1 Antibody (NB600-261)(3)

Review for Ctip1 Antibody (NB600-261) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow.

Reviews using NB600-261:
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Flow
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Zhiwei Zhou
Flow Mouse 09/26/2012
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Summary

ApplicationFlow Cytometry
Sample Testedmouse B cell
SpeciesMouse
LotA2

Blocking

Blocking Details2%BSA

Primary Anitbody

Dilution Ratio1ug/ml

Details

Fixation Details4% PFA
Permeabilization Details0.1%Triton 100
Wash DescriptionBD Wash buffer

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for Ctip1 Antibody (NB600-261) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Ctip1 Antibody (NB600-261)

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Recent Reviews

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5
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4
1
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2
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1
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Zhiwei Zhou
09/26/2012
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol BCL11A
Entrez
Uniprot