Ctip1 Antibody - BSA Free

Western Blot: Ctip1 Antibody [NB600-261] - Whole cell lysate (50 ug) from L1210, TCMK-1, and 4T1 cells. Antibodies: Affinity purified rabbit anti-Bcl11a antibody used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: Ctip1 Antibody [NB600-261] - Detection of human Ctip1 (Bcl11a) by western blot. Samples: Whole cell lysate from Ramos (15 and 50 ug), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Ctip1 antibody NB600-261 used at 1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds.

Immunoprecipitation: Ctip1 Antibody [NB600-261] - Detection of human Ctip1 (Bcl11a) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells. Antibodies: Affinity purified rabbit anti-Ctip1 antibody NB600-261 used for IP at 6 ul/mg lysate. Ctip1 was also immunoprecipitated by a rabbit anti-Ctip1 antibodies (NB600-258, NB600-259) and rabbit anti-Ctip1 recombinant monoclonal antibody [BLR073G] (NBP2-78710). For blotting immunoprecipitated Ctip1, NBP2-78710 was used at 1:1000. Chemiluminescence with an exposure time of 75 seconds.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IP, ChIP, GS
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Ctip1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide which maps to a region between residues 775 and the C-terminus (residue 835) of human B-cell CLL/Lymphoma 11A using the numbering given in Swiss-Prot entry Q9H165 (GeneID 53335).
• Specificity: NB600-261 is specific for human Bcl-11a protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL11A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Flow Cytometry 1:10-1:1000
  • Gel Supershift Assay
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:1000-1:5000
• Application Notes: Ctip1 antibody validated for Flow from a verified customer review. Use in Chromatin Immunoprecipitation was reported in scientific publication (PMID: 23741395). Use in Gel suppershift assay reported in scientific literature (PMID:34341563).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ctip1 Antibody - BSA Free

• B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2
• B-cell CLL/lymphoma 11A (zinc finger protein)
• B-cell CLL/lymphoma 11A
• B-cell lymphoma/leukemia 11A
• BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1
• BCL-11A
• BCL11A-S
• BCL11A-XL
• C2H2-type zinc finger protein
• COUP-TF-interacting protein 1
• CTIP1ecotropic viral integration site 9
• ecotropic viral integration site 9 homolog
• Ecotropic viral integration site 9 protein homolog
• EVI-9
• EVI9FLJ34997
• HBFQTL5
• KIAA1809BCL11A-L
• Zinc finger protein 856
• ZNF856FLJ10173

Background

The B cell leukemia 11A protein (BCL11A/Evi9/CTIP1) has been implicated in hematopoietic cell development and malignancies. BCL11A is a transcriptional repressor that binds directly to a GC-rich motif and is also recruited to a promoter template via interaction with the orphan nuclear receptor, chicken ovalbumin upstream promoter transcription factor II. In both cases, BCL11A-mediated transcriptional repression is only minimally reversed by trichostatin A, suggesting the possible lack of involvement of class I or II histone deacetylases. Nonetheless, chromatin immunoprecipitation assays revealed that expression of BCL11A in mammalian cells resulted in deacetylation of histones H3 and/or H4 that were associated with the promoter region of a reporter gene. BCL11A mediated transcriptional repression, as well as deacetylation of histone H3/H4 in BCL11A-transfected cells, was partially reversed by nicotinamide, an inhibitor of class III histone deacetylases such as SIRT1. SIRT1 was found to interact directly with BCL11A and was recruited to the promoter template in a BCL11A-dependent manner leading to transcriptional repression. These findings indicate a role for SIRT1 in transcriptional repression mediated by BCL11A in mammalian cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ctip1 Antibody (NB600-261)(10)

Publications using NB600-261

• Woollett K Is there a transcription factor code? First steps towards assessing temporal changes in the landscape of post-transcriptional modifications during cellular differentiation Thesis 2023-01-01 (WB)
• Khan DMIO, Karmaus PWF, Bach A et al. An in vitro model of human hematopoiesis identifies a regulatory role for the aryl hydrocarbon receptor Blood advances 2023-07-21 [PMID: 37477592]
• S Takase, T Hiroyama, F Shirai, Y Maemoto, A Nakata, M Arata, S Matsuoka, T Sonoda, H Niwa, S Sato, T Umehara, M Shirouzu, Y Nishigaya, T Sumiya, N Hashimoto, R Namie, M Usui, T Ohishi, SI Ohba, M Kawada, Y Hayashi, H Harada, T Yamaguchi, Y Shinkai, Y Nakamura, M Yoshida, A Ito A specific G9a inhibitor unveils BGLT3 lncRNA as a universal mediator of chemically induced fetal globin gene expression Nature Communications, 2023-01-12;14(1):23. 2023-01-12 [PMID: 36635268]
• Doerfler PA, Feng R, Li Y Et al. Activation of gamma-globin gene expression by GATA1 and NF-gamma in hereditary persistence of fetal hemoglobin Nature genetics 2021-08-01 [PMID: 34341563] (GS, Human)
• Fugazza C, Barbarani G, Elangovan S et Al. The Coup-TFII orphan nuclear receptor is an activator of the gamma -globin gene Haematologica 2020-02-27 [PMID: 32107331]
• Martyn GE, Wienert B, Kurita R et al. A natural regulatory mutation in the proximal promoter elevates fetal globin expression by creating a de novo GATA1 site. Blood 2019-01-07 [PMID: 30617196] (Chemotaxis, Human)
• Martyn GE, Wienert B, Yang L et al. Natural regulatory mutations elevate the fetal globin gene via disruption of BCL11A or ZBTB7A binding. Nat. Genet. 2018-04-01 [PMID: 29610478] (WB)
• Zhu X, Hu T, Ho MH et al. Hydroxyurea differentially modulates activator and repressors of g-globin gene in erythroblasts of responsive and non-responsive patients with sickle cell disease in correlation with Index of Hydroxyurea Responsiveness. Haematologica. 2017-09-29 [PMID: 28971909] (Human)
• Wu X, Satpathy AT, Kc W et al. Bcl11a Controls Flt3 Expression in Early Hematopoietic Progenitors and Is Required for pDC Development In Vivo. PLoS One 2013-05-31 [PMID: 23741395] (Chemotaxis, Mouse)
• J Xu, V G Sankaran, M Ni et al. Transcriptional silencing of {gamma}-globin by BCL11A involves long-range interactions and cooperation with SOX6. Genes & Dev; 24 (8): 783-798. 2010-01-01 [PMID: 20395365]

Review for Ctip1 Antibody (NB600-261) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Zhiwei Zhou Flow Mouse 09/26/2012

Summary

• Application: Flow Cytometry
• Sample Tested: mouse B cell
• Species: Mouse
• Lot: A2

Bioinformatics

• Gene Symbol: BCL11A
• Entrez:
  • 53335(Human)
• Uniprot:
  • Q9H165(Human)