| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56095SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-527SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76639-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56368SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33076 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56101SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56311SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56096SS | |||
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330] - 293T cells stained with Cyclin A2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunofluorescence-NBP1-31330-img0010.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug Neuro2A whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0006.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug PC-12 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0007.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0013.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Cyclin A2 Antibody [NBP1-31330] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0014.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0016.jpg)
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330] - NCIN87 Xenograft, using cyclin A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunohistochemistry-Paraffin-NBP1-31330-img0004.jpg)
![Immunoprecipitation: Cyclin A2 Antibody [NBP1-31330] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of Cyclin A2 protein by 2. 5 ug of Cyclin A2 antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunoprecipitation-NBP1-31330-img0009.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young & old MRC-5 & HFF fibroblasts measured by RNA-seq, qRT-PCR, & Western Blots. (a) Four genes commonly downregulated & (b) 8 genes commonly upregulated in both cell lines. (a, b) The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Solid colored bars represent MRC-5 while shaded boxes represent HFF cells. The height of the bars corresponds to the logarithmic fold-change (FC) of expression between the first & the last PD investigated here (RNA-seq: log 2 RPKM FC; qRT-PCR: log 2− delta delta CT; protein: log 2 expression ratio). Error bars indicate standard deviation from the mean. Changes statistically different comparing young & old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: ∗p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-31330_rabbit-polyclonal-cyclin-a2-antibody-immunocytochemistry-immunofluorescence-141120251833319.jpg)
![Western Blot: Bax Antibody [NB100-56095] - Total protein from human THP1, A431, A549 and U2OS cells was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:2000 dilution of anti-BAX in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56095-img0006.jpg "Western Blot: Bax Antibody [NB100-56095] - Total protein from human THP1, A431, A549 and U2OS cells was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:2000 dilution of anti-BAX in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence")
![Immunohistochemistry-Paraffin: Bax Antibody [NB100-56095] - Formalin-fixed, paraffin-embedded sections of human colon cancer fom a tissue microarray stained for BAX using NB100-56095 at 1:2000. A. High Bax expression. B. Low Bax expression. Hematoxylin-eosin counterstain](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NB100-56095-img0007.jpg)
![Western Blot: Bax Antibody [NB100-56095] - Analysis of BAX in colon tumor cell lines using this antibody. Bax is detected in the SW620 and DLD-1 cell lines. However, Bax was not detected in the cell lines (LoVo, DU145, and LS180) known to have frameshift mutations in Bax (Rampino et al. 1997). An antibody to F1ATPase was used as a protein loading control.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56095-img0004.jpg)
![Western Blot: Bax Antibody [NB100-56095] - Analysis of Bax in Daoy whole cell lysate using anti-Bax antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56095-img0005.jpg)
![Western Blot: VEGF R1/Flt-1 Antibody [NB100-527] - Chimeric CSF-1R/VEGFR-2 detection in transfected lysates.](http://images.novusbio.com/fullsize/VEGF-R1-Flt-1-Antibody-Western-Blot-NB100-527-img0005.jpg "Western Blot: VEGF R1/Flt-1 Antibody [NB100-527] - Chimeric CSF-1R/VEGFR-2 detection in transfected lysates.")
![Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody [NB100-527] - VEGF R1/Flt-1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/VEGF-R1-Flt-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-527-img0006.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells (punctate appearance typical of receptors). The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-Immunohistochemistry-Paraffin-NB100-527-img0009.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of a FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells. The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-Immunohistochemistry-Paraffin-NB100-527-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - K562 cell lysate with PUMA antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NBP1-76639-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 20 ug of lysates per lane.Antibodies: NBP1-76654 (3 ug/mL), NBP1-76639 (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NBP1-76639-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76639_rabbit-polyclonal-puma-antibody-271120242016915.gif)
![Immunocytochemistry/ Immunofluorescence: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in K562. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing cytosol staining on K562 cells](http://images.novusbio.com/fullsize/nbp1-76639_rabbit-polyclonal-puma-antibody-271120242045643.jpg)
![Immunohistochemistry: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in Human Breast Carcinoma. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76639_rabbit-polyclonal-puma-antibody-2711202420243374.jpg)
![Immunohistochemistry: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in Human Breast Tissue. Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PUMA antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76639_rabbit-polyclonal-puma-antibody-2711202420285891.gif)
![Western Blot: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of PIM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: BT-20 cell lysateLane 2: HepG2 cell lysate Lane 3: SW480 cell lysate](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Western-Blot-NBP2-67528-img0010.jpg "Western Blot: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of PIM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: BT-20 cell lysateLane 2: HepG2 cell lysate Lane 3: SW480 cell lysate")
![Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunocytochemistry-Immunofluorescence-NBP2-67528-img0009.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human tonsil tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunocytochemistry-Immunofluorescence-NBP2-67528-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunocytochemistry-Immunofluorescence-NBP2-67528-img0008.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human breast carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0002.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human colon cancer tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0003.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human gastric carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0004.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human spleen tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0005.jpg)
![Western Blot: Noxa Antibody [NB100-56368] - Noxa expression was stably reduced in HCT116 cells by shRNA. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Noxa-Antibody-Western-Blot-NB100-56368-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Noxa Antibody [NB100-56368] - Noxa expression was stably reduced in HCT116 cells by shRNA. Western blot image submitted by a verified customer review.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Noxa Antibody [NB100-56368] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using Noxa antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Noxa-Antibody-Immunohistochemistry-Paraffin-NB100-56368-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Noxa Antibody [NB100-56368] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Noxa (NB100-56368) antibody at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Noxa-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56368-img0003.jpg)
![Western Blot: Noxa Antibody [NB100-56368] - Western blot analysis of Noxa in A) human, B) mouse, and C) rat thymus tissue using Noxa antibody NB100-56368 at 2 ug/mL.](http://images.novusbio.com/fullsize/Noxa-Antibody-Western-Blot-NB100-56368-img0001.jpg)
![Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Whole cell extracts (30 and 50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Western-Blot-NB100-404-img0003.jpg "Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Whole cell extracts (30 and 50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody at a dilution of 1:500.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (6B4) [NB100-404] - Staining of BRCA1 nuclear foci induced by ionizing radiation. IR-treated (2 hr /4 gray IR) U2OS cells were pre-extracted with CSK buffer on ice for 4 min before fixation with 4% PFA in room temperature, and then subjected to immunostaining. DAPI was used to counterstain nucleus. 6B4 was used. Secondary antibody (Alexa Fluor-488) used for detection of primary antibody (BRCA1 antibody 6B4)](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Immunocytochemistry-Immunofluorescence-NB100-404-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade diluted at 1:500.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Western-Blot-NB100-404-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - NB100-404 was used at 1:1000 dilution for Western blot assay of lysates from cells transfected with control or BRCA1-specific siRNA. Lysates were prepared at the indicated times following transfection. RAD50 antibody (13B3) (NB100-147) was used as a loading control.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Western-Blot-NB100-404-img0004.jpg)
![Immunoprecipitation: BRCA1 Antibody (6B4) [NB100-404] - 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Immunoprecipitation-NB100-404-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody, diluted at 1:500. A HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Knockout-Validated-NB100-404-img0015.jpg)
![Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (NB100-404) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/nb100-404_mouse-monoclonal-brca1-antibody-6b4-91020242091412.jpg)
![Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Analysis using the PE conjugate of NBP2-29455. Surface Staining of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 5 ul/0.5 ug/10^6 cells. PPI negative cell population was gated for analysis.](http://images.novusbio.com/fullsize/CD34-Antibody-ICO-115-Flow-Cytometry-NBP2-29455-img0002.jpg "Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Analysis using the PE conjugate of NBP2-29455. Surface Staining of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 5 ul/0.5 ug/10^6 cells. PPI negative cell population was gated for analysis.")
![Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/CD34-Antibody-ICO-115-Flow-Cytometry-NBP2-29455-img0001.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56101] - Analysis of Bcl-2 in 293T cell lysate (30ug) using anti-Bcl-2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NB100-56101-img0002.jpg "Western Blot: Bcl-2 Antibody [NB100-56101] - Analysis of Bcl-2 in 293T cell lysate (30ug) using anti-Bcl-2 antibody. Image from verified customer review.")
![Western Blot: SMAC/Diablo Antibody [NB100-56311] - Analysis of Smac (DIABLO) in HeLa lysate using Smac antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Western-Blot-NB100-56311-img0006.jpg "Western Blot: SMAC/Diablo Antibody [NB100-56311] - Analysis of Smac (DIABLO) in HeLa lysate using Smac antibody at 2 ug/ml.")
![Immunohistochemistry: SMAC/Diablo Antibody [NB100-56311] - Analysis of human prostate using Smac antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Immunohistochemistry-NB100-56311-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20535-img0006.jpg "Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535] - Human breast carcinoma. SOD2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP2-20535-img0014.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-20535_rabbit-polyclonal-sod2-mn-sod-antibody-1610202419405789.jpg)
![Western Blot: Bax Antibody [NB100-56096] - Analysis of Bax in mouse using this antibody. 1. In vitro translated mouse Bax. 2. Normal cortex. 3. Ischemic hippocampus. 4. Ischemic hippocampus. Ishemic samples were prepared 6 hr post middle cerebral artery occlusion. The results show the Bax expression increased following ischemia.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56096-img0003.jpg "Western Blot: Bax Antibody [NB100-56096] - Analysis of Bax in mouse using this antibody. 1. In vitro translated mouse Bax. 2. Normal cortex. 3. Ischemic hippocampus. 4. Ischemic hippocampus. Ishemic samples were prepared 6 hr post middle cerebral artery occlusion. The results show the Bax expression increased following ischemia.")
![Immunohistochemistry-Paraffin: Bax Antibody [NB100-56096] - Mouse tissue sections stained for Bax using NB100-56096 at 1:2000. A. Pancreas. B. Brain showing expression of Bax in the Purkinge cells.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NB100-56096-img0002.jpg)