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Ctip1 Antibody [HRP]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB600-261AF350
Alexa Fluor 405
NB600-261AF405
Alexa Fluor 488
NB600-261AF488
Alexa Fluor 532
NB600-261AF532
Alexa Fluor 594
NB600-261AF594
Alexa Fluor 647
NB600-261AF647
Alexa Fluor 700
NB600-261AF700
Alexa Fluor 750
NB600-261AF750
Allophycocyanin
NB600-261APC
Biotin
NB600-261B
DyLight 350
NB600-261UV
DyLight 405
NB600-261V
DyLight 488
NB600-261G
DyLight 550
NB600-261R
DyLight 594
NB600-261DL594
DyLight 650
NB600-261C
DyLight 680
NB600-261FR
DyLight 755
NB600-261IR
FITC
NB600-261F
Janelia Fluor 549
NB600-261JF549
Janelia Fluor 646
NB600-261JF646
PE
NB600-261PE
PerCP
NB600-261PCP
Unconjugated
NB600-261
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Ctip1 Antibody [HRP] Summary

Immunogen
A synthetic peptide which maps to a region between residues 775 and the C-terminus (residue 835) of human B-cell CLL/Lymphoma 11A using the numbering given in Swiss-Prot entry Q9H165 (GeneID 53335).
Specificity
NB600-261 is specific for human Bcl-11a protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL11A
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Ctip1 Antibody [HRP]

  • B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2
  • B-cell CLL/lymphoma 11A (zinc finger protein)
  • B-cell CLL/lymphoma 11A
  • B-cell lymphoma/leukemia 11A
  • BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1
  • BCL-11A
  • BCL11A-S
  • BCL11A-XL
  • C2H2-type zinc finger protein
  • COUP-TF-interacting protein 1
  • CTIP1ecotropic viral integration site 9
  • ecotropic viral integration site 9 homolog
  • Ecotropic viral integration site 9 protein homolog
  • EVI-9
  • EVI9FLJ34997
  • HBFQTL5
  • KIAA1809BCL11A-L
  • Zinc finger protein 856
  • ZNF856FLJ10173

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: BCL11B Antibody [NB100-2600] - Section of human skin tumor (higher magnification). Antibody: Affinity purified rabbit anti-Bcl11b used at a dilution of 1:500. Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1:100.
Immunohistochemistry-Paraffin: BCL11B Antibody [NB100-2600] - P7 brain mouse section was stained with anti-BCL11B antibody. Image from verified customer review.
Immunohistochemistry: BCL11B Antibody [NB100-2600] - Immunohistochemistry and FISH of AML cases. Immunohistochemistry analysis of BCL11B-expressing AML samples #59810, #57863 and #13 carrying t(2;14)(q22.3;q32.3), t(7;14)(q21q32) and no 14q32 alteration, respectively. BCL11B expression was detected in samples regardless of the presence of the 14q32 alterations. The expression was limited to the nucleus and the percentage of positive neoplastic cells was always >= 50%. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2072-6694/11/12/1951), licensed under a CC-BY licence.
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Immunocytochemistry/Immunofluorescence: Hemoglobin beta Antibody [NBP2-14081] - Staining of human cell line Hep G2 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Hemoglobin beta Antibody [NBP2-14081] - Staining of human placenta shows erythrocyte positivity in vessels.
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KLF1 Antibody
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NBP2-37380
Western Blot: KLF1 Antibody (1B6A3) [NBP2-37380] - Western blot analysis using KLF1 mAb against human KLF1 recombinant protein. (Expected MW is 42.6 kDa)
Immunohistochemistry: KLF1 Antibody (1B6A3) [NBP2-37380] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using KLF1 mouse mAb with DAB staining.
Flow Cytometry: KLF1 Antibody (1B6A3) [NBP2-37380] - Flow cytometric analysis of HeLa cells using KLF1 mouse mAb (green) and negative control (red).
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
MAP1B [p Thr1265]
Antibody - SuperBUGS
NBP1-42827
Western Blot: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Analysis of phosphorylated MAP1B in chick embryos (lane 1) and rat neonatal brain (lane 2).
Immunohistochemistry: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Immunohistochemical analysis of rat spinal cord transverse section from an E12 embryo.
Western Blot: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Western blot analysis of p62, Nbr1, MAP1B-HC, and MAP1B-LC1 protein levels following blockage of autophagic protein degradation. Phospho-MAP1B-HC is degraded by autophagy. Upon blockage of autophagic degradation with Bafilomycin A1: (Baf), levels of phospho-MAP1B-HC increase compared with levels of total MAP1B-HC. Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/ijcb/2012/208014/), licensed under a CC-BY licence.
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Species: Hu, Mu, Rt, Ch, Fi, Re, Xp
Applications: WB, ICC/IF, IHC, IHC-Fr
Ovalbumin Antibody
NB600-922
Western Blot: Ovalbumin Antibody [NB600-922] - Lane 1: Reduced Ovalbumin. Load: 50 ng. Primary antibody: Ovalbumin antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin.
Western Blot: Ovalbumin Antibody [NB600-922] - Western blot detection of Ovalbumin from hen egg white using NB600-922 at 1:10,000. The blot was blocked ON at 4 degrees C in 3% BSA. The secondary antibody used was a DyLight 649 Conjugated Goat anti-rabbit IgG (H&L) diluted 1:20,000 and incubated 1 hr RT in blocking buffer for fluorescent western blotting.
Western Blot: Ovalbumin Antibody [NB600-922] - Analysis using the HRP conjugate of NB600-922. Detection of Lane 1: Ovalbumin (Hen Egg). Load: 50 ng per lane. Dilution: 1:1,000. Predicted/Observed size: 43 kDa, 43 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.
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GATA-1 Antibody
(4F5)
NBP1-47492
Western Blot: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis using GATA1 mouse mAb against K562 (1) cell lysate.
Immunocytochemistry/Immunofluorescence: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of K562(left) cells using GATA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: GATA-1 Antibody (4F5) [NBP1-47492] - Staining of Fetal Baboon Liver. Antigen retrieval: 10mM sodium citrate, pH 6.0, Primary Ab: 1:500 dilution, Secondary Ab (biotinylated): 1:200, Streptavidin-AlexaFluor488: 1:500 (green), Quench autofluorescence with 1% Sudan Black in 70% methanol. Image from verified customer review.
(1 Review)
(2 Publications)
Species: Hu, Pm
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P
Sirtuin 1/SIRT1
Antibody (1F3)
NBP1-51641
Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates.
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Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, Flow-IC, IHC-FrFl
Bcl-6 Antibody
NBP2-59786
Immunocytochemistry/Immunofluorescence: Bcl-6 Antibody [NBP2-59786] - Human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Bcl-6 Antibody [NBP2-59786] - Staining of human lymphoma, non-Hodgkin's type, shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: Bcl-6 Antibody [NBP2-59786] - Human tonsil shows strong nuclear positivity in germinal center cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Bcl-2 Antibody
NB100-56098
Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with (1) scrambled control siRNA or (2) Bcl-2 siRNA. Image from verified customer review.
Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of whole cell lysate (WCL) of Jurkat cells using anti-Bcl-2 antibody (NB100-56098) at 1:1000 dilution. HRP conjugated goat anti-rabbit IgG (H+L) cross adsorbed secondary antibody was used with ECL substrate for the detection of Bcl2 antibody bound to the blotted protein. This Bcl2 antibody detected a specific band at expected molecular weight marker position of Bcl2 protein (26kDa).
Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56098] - Analysis of a FFPE human breast carcinoma tissue section using 1:1000 dilution of Bcl-2 antibody (NB100-56098) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of BCL2 in the cancer cells with relatively intense signal in their nuclear membranes. Staining was performed by Histowiz.
(2 Reviews)
(10 Publications)
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NBP1-80306
Western Blot: c-Myb Antibody [NBP1-80306] - WB Suggested Anti-MYB Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate
Immunohistochemistry-Paraffin: c-Myb Antibody [NBP1-80306] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: c-Myb Antibody [NBP1-80306] - c-Myb in MOLT-4 cells transfected with siRNA. Image from verified customer review.
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(2 Publications)
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DCC Antibody
AF844
DCC Antibody
(1 Review)
(6 Publications)
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Applications: WB, Block
c-Myc Antibody
NB600-335
Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody NB600-335 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the Alexa Fluor 488 format of this antibody.
Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ug/ml (1:25,000).
(1 Review)
(49 Publications)
Species: Hu, Mu, Ec, All-NA
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(14 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Histone H3
[Trimethyl Lys9] Anti ...
NBP1-30141
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.
Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Histone 3 was detected in immersion fixed paraffin-embedded sections of human colon cancer using anti-human mouse monoclonal antibody (Catalog # NBP1-30141) at 1:1000 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 [Trymethyl Lys9] (6F12-H4) at 2 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.
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Flt-3/Flk-2/CD135
Antibody (7E8.2C8)
NBP2-42210
Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position.
Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver cancer using Flt-3/Flk-2/CD135 antibody (clone 7E2.2C8) at 15 ug/ml concentration. The antibody generated specific cytoplasmic immunostaining of FLT3 in the cancerous cells while the tumor stroma was largely negative for the staining.
Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - HEK293 cells were stained (surface) with Flt-3/Flk-2/CD135 antibody (clone: 7E8.2C8; red) or isotype control (mouse IgG2 lambda; blue) and positive staining observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis
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Applications: WB, Flow, IHC, IHC-P
TLX/NR2E1 Antibody
(H6506)
PP-H6506-00
TLX/NR2E1 Antibody (H6506)
Species: Hu
Applications: WB, IP, DirELISA
p53 Antibody (PAb
240)
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
(3 Reviews)
(28 Publications)
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
Ctip1 Antibody
[HRP]
NB600-261H
Ctip1 Antibody [HRP]
Species: Hu, Mu
Applications: WB

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Product General Protocols

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FAQs for Ctip1 Antibody (NB600-261H) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-261AF350
Alexa Fluor 405 NB600-261AF405
Alexa Fluor 488 NB600-261AF488
Alexa Fluor 532 NB600-261AF532
Alexa Fluor 594 NB600-261AF594
Alexa Fluor 647 NB600-261AF647
Alexa Fluor 700 NB600-261AF700
Alexa Fluor 750 NB600-261AF750
Allophycocyanin NB600-261APC
Biotin NB600-261B
DyLight 350 NB600-261UV
DyLight 405 NB600-261V
DyLight 488 NB600-261G
DyLight 550 NB600-261R
DyLight 594 NB600-261DL594
DyLight 650 NB600-261C
DyLight 680 NB600-261FR
DyLight 755 NB600-261IR
FITC NB600-261F
HRP NB600-261H
Janelia Fluor 549 NB600-261JF549
Janelia Fluor 646 NB600-261JF646
PE NB600-261PE
PerCP NB600-261PCP
Learn about Custom Antibody Labeling

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Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody NB600-335 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
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Bioinformatics

Gene Symbol BCL11A
Entrez
Uniprot