Signal Transduction reagents: Novus Biologicals

NB600-600

GLI-1 Antibody - BSA Free

(1 Review)

(58 Publications)

0.1 ml

NB600-600

Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Staining in canine hair follicle. Image from a verified customer review.

Flow (Intracellular): GLI-1 Antibody [NB600-600] - Staining for GLI-1 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-1 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a negative control. Image courtesy of Sherine Elsawa, Northern Illinois University.

Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600] - Paracrine signaling of tumour-derived SHH in CAFs promotes lymphangiogenesis in vivo(A) Growth properties of subcutaneous tumours in mice derived from Caov3 SHH-OE or Caov3 NC cells alone or co-injected with CAFs. Kaplan-Meier plot of time to tumour progression, as evidenced by survival rates over time (n=5). (B) Bright field images of subcutaneous tumours from each group (n=5) 20 days after tumour implantation. (C) Graphical representation of the weights of subcutaneous tumours from each group 20 days after tumour implantation. (D) Immunohistochemical staining for Ki-67, alpha -SMA, Gli-1 & LYVE1 in tumour sections from mice in each group. (E) Graphical representation of the number of LYVE1+ vessels counted from IHC images in panel D. (*P<0.05, **P<0.01, ***P<0.001). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.18621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600] - Inhibition of Hh signaling in CAFs blocks its effects on lymphangiogenesis in vivo & in vitro(A) Representative images & statistical analyses of migration of LECs in a co-culture invasion system with CAFs stably transfected with shNC or Gli-shRNA lentivirus in the presence of rSHH (1 μg/ml). (B) Representative images & statistical analyses of capillary tube formation of LECs treated with supernatants from cultures of CAFs stably transfected with shNC or Gli-shRNA lentivirus in the presence of rSHH (1 μg/ml). (C) Bright field images & graphical representation of the weights of tumours of subcutaneous tumours from mice (n=5) bearing Caov3 SHH-OE cells co-injected with CAFs stably transfected with shNC or Gli-shRNA lentivirus 20 days after tumour implantation. (D) Growth properties of subcutaneous tumours in mice derived from Caov3 SHH-OE cells co-injected with CAFs stably transfected with shNC or Gli-shRNA lentivirus. Kaplan-Meier plot of time to tumour progression, as evidenced by survival rates over time. (E) IHC staining for Ki-67, alpha -SMA, Gli-1 & LYVE1 in tumour sections from mice in the two groups. (F) Graphical representation of the number of LYVE1+ vessels counted from IHC images in panel E. (**P<0.01, ***P<0.001). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.18621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Comparison of SHH and GLI-1 expression in the normal mammary gland and MGT tissues. A For western blotting, the blots were cut prior to hybridisation with antibodies. SHH (35 kDa) and GLI-1 (100–140 kDa) protein levels in the normal mammary gland and benign and malignant mammary gland tissues. Beta-actin was used as the loading control. B Relative quantitation of SHH and (C) GLI-1 proteins were normalized to that of the control group. The mean and standard error of the mean are used to express quantitative data. (*, **, #: p < .05). SHH, sonic hedgehog; GLI-1, glioma-associated oncogene 1; MGT, mammary gland tumour Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37932728), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Ca

Species Glossary

Reactivity: Hu, Mu, Ca

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-600AF350
Alexa Fluor 405	NB600-600AF405
Alexa Fluor 488	NB600-600AF488
Alexa Fluor 532	NB600-600AF532
Alexa Fluor 594	NB600-600AF594
Alexa Fluor 647	NB600-600AF647
Alexa Fluor 700	NB600-600AF700
Alexa Fluor 750	NB600-600AF750
Allophycocyanin	NB600-600APC
Biotin	NB600-600B
CoraFluor 1	NB600-600CL1
DyLight 350	NB600-600UV
DyLight 405	NB600-600V
DyLight 488	NB600-600G
DyLight 550	NB600-600R
DyLight 594	NB600-600DL594
DyLight 650	NB600-600C
DyLight 680	NB600-600FR
DyLight 755	NB600-600IR
FITC	NB600-600F
HRP	NB600-600H
Janelia Fluor 525	NB600-600JF525
Janelia Fluor 549	NB600-600JF549-100ul
Janelia Fluor 646	NB600-600JF646-100ul
Janelia Fluor 669	NB600-600JF669
PE	NB600-600PE
PerCP	NB600-600PCP
mFluor Violet 450 SE	NB600-600MFV450
mFluor Violet 500 SE	NB600-600MFV500
mFluor Violet 610 SE	NB600-600MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-600SS

compare

(select up to 3 products from this page to compare)

NBP2-33006

alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free

(2 Reviews)

(54 Publications)

0.1 mg

NBP2-33006

alpha -Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human/Mouse/Rat alpha -Smooth Muscle Actin Monoclonal Antibody (Novus Catalog # NBP2-33006) at 0.25ug/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cytoskeleton. Protocol available in COMET™ Panel Builder.

alpha -Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human/Mouse/Rat alpha -Smooth Muscle Actin Monoclonal Antibody (Novus Catalog # NBP2-33006) at 0.25ug/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cytoskeleton. Protocol available in COMET™ Panel Builder.

alpha -Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human breast cancer using Mouse Anti-Human/Mouse/Rat alpha -Smooth Muscle Actin Monoclonal Antibody (Novus Catalog # NBP2-33006) at 0.25ug/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cytoskeleton. Protocol available in COMET™ Panel Builder.

SMA Antibody was detected in immersion fixed paraffin-embedded sections of mouse Lung using Mouse Anti-Human/Mouse SMA, Monoclonal Antibody (Catalog #NBP2-33006) at 0.25ug/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Western blot shows lysates of human heart tissue and iBJ6 human induced pluripotent stem cell line undifferentiated or cardiac differentiated. PVDF membrane was probed with 0.25 ug/mL of Mouse Anti-alpha-Smooth Muscle Actin Monoclonal Antibody (Catalog # NBP2-33006) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). The observed molecular weight was detected for alpha Smooth Muscle Actin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.

Feline tissue stained with alpha-Smooth Muscle Actin Antibody at dilution of 1:600. Vascular wall was stained. IHC image submitted by a verified customer review.

Simple Western lane view shows a specific band for an observed molecular weight at ~45 kDa for alpha-Smooth Muscle Actin in 0.2 mg/ml of h. Aorta lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

An intracellular stain was performed on HeLa cells with NBP2-34522AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Canine brain/spinal cord section. Alpha-Smooth Muscle Actin Antibody (1A4/asm-1) staining in red. Endothelial cell marker in green. DAPI in blue. Alexa Fluor conjugated antibodies used for secondary antibodies. IHC image submitted by a verified customer review.

Whole cell lysates from SUM159, MDA-MB-231 and MCF-7 cells were loaded with 50 ug/lane. 10% SDS-PAGE. Alpha-Smooth Muscle Actin antibody (NBP2-33006) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.

alpha-Smooth-Muscle-Actin-Antibody-1A4-asm-1-Immunohistochemistry-Paraffin-NBP2-33006-img0017.jpg

An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

Staining of human Leiomyosarcoma statined with the Azide and BSA-free version of alpha-Smooth Muscle Actin Antibody (1A4/asm-1).

An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image from the Alexa Fluor 647 version of this antibody.

Simple Western lane view shows a specific band for an observed molecular weight for Smooth Muscle Actin in 0.2 mg/ml of h. Uterus lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Electropherogram image of the corresponding Simple Western lane. alpha-Smooth Muscle Actin antibody was used at 10 ug/ml dilution of h. Aorta and h. Uterus lysates(s) respectively.

Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free [NBP2-33006] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.

Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free [NBP2-33006] - Human tonsil frozen tissue section stained for alpha-Smooth Muscle Actin (red) with the Janelia Fluor 549 conjugate of the Azide and BSA-Free version of alpha-Smooth Muscle Actin Antibody (1A4/asm-1), and counterstained with DAPI (blue). Image from verified customer review.

Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free [NBP2-33006] - alpha-Smooth Muscle Actin expression in colorectal tumor cells. Using Allophycocyanin conjugated version of antibody (NBP2-34522APC). Image from verified customer review.

Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free [NBP2-33006] - Analysis of alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [Alexa Fluor® 647] (Catalog Number: NBP2-34522AF647) using two lung cancer cell lines to acquire the subcutaneous tumors. MMT status of macrophages was then analyzed. Image from a verified customer review.

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free [NBP2-33006] - aSMA stained over night in human FFPE tonsil section ((Catalog # NBP2-33006AF750)). Heat mediated antigen retrieval at pH 9. 1:50 Dilution in 3% BSA, incubation over night at 4 °C. Image from a verified customer review.

Immunohistochemistry-Paraffin: Mouse Monoclonal alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Images demonstrating SMA immunoreactivity in a variety of human FFPE tissue sections. NBP2-33006 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature. Formalin fixed paraffin embedded sections were deparaffinized and rehydrated. Sections then underwent heat induced epitope retrieval in a citrate-based solution for 5min in a food steamer. Endogenous peroxidase activity was blocked by incubating slides for 15min in tris buffered saline containing 3% (volume/volume) hydrogen peroxide. Tissue sections were then washed, blocked with normal horse serum for 20 min, and then incubated with the primary antibody (125ng/mL) for 30min at room temperature. Sections were then washed and incubated with a horse anti-mouse HRP polymer for 30m at room temperature. Sections were washed and incubated with DAB chromogen for approximately 2.5min. Sections were then washed, counterstained with hematoxylin, dehydrated, cleared and mounted with a coverslip. Image from a verified customer review.

Immunohistochemistry-Paraffin: Mouse Monoclonal alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - SMA in FFPE sections of pig tissue. Formalin fixed paraffin embedded sections were deparaffinized and rehydrated. Sections then underwent heat induced epitope retrieval in a citrate-based solution for 5min in a food steamer. Endogenous peroxidase activity was blocked by incubating slides for 15min in tris buffered saline containing 3% (volume/volume) hydrogen peroxide. Tissue sections were then washed, blocked with normal horse serum for 20 min, and then incubated with the primary antibody (125ng/mL) for 30min at room temperature. Sections were then washed and incubated with a horse anti-mouse HRP polymer for 30m at room temperature. Sections were washed and incubated with DAB chromogen for approximately 2.5min. Sections were then washed, counterstained with hematoxylin, dehydrated, cleared and mounted with a coverslip. Image from a verified customer review.

SMA immunoreactivity in an FFPE section of cow lung. NBP2-33006 was diluted to 125ng/mL and was left on tissue sections for 30min at room temperature. Image from a verified customer review.

Large-conductance and Ca2+-activated K+ (BK) channels are expressed and functional in activated hepatic stellate cells (HSCs). (A,B) Representative RT-qPCR (A) and western blots (B) showing the expression of ACTA2 and the BK channels alpha subunit KCNMA1 in activated human LX2 cells treated with transforming growth factor beta 1 (TGF beta 1) and spontaneously activated primary rat HSCs in vitro. (C) Representative immunofluorescence images of KCNMA1 and ACTA2 in activated primary rat HSCs (Scale bars, 25 μm). (D,E) Representative whole-cell K+ current traces and the normalized current recorded from activated HSCs before and after treatment with rottlerin (Rot) and paxilline (Pax), as indicated at 1 μM internal Ca2+. The whole-cell currents were elicited by 1 s voltage ramps from −100 to 80 mV. Rottlerin (1 μM) and paxilline (10 μM) were freshly prepared from stock solutions and the final DMSO concentration was 0.1% (*p < 0.05 compared with the vehicle group, n = 3). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32210801), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

M2 macrophage-related protein expression and the co-location of M2-macrophages and GC-MSCs within tumor tissues.a Expression levels of iNOS, Ym-1, and Fizz-1 mRNA were analyzed by RT-qPCR in tumor tissues and the corresponding adjacent non-cancerous tissues collected randomly from 10 gastric cancer patients. b The expressions of arginase-1 and CCR-2 were assessed by western blot in tumor tissues and the corresponding adjacent non-cancerous tissues collected randomly from 10 gastric cancer patients. c Representative immumofluoresence images of the co-location of GC-MSCs ( alpha -SMA; green) and M2-macrophages (CD204; red) in gastric cancer tissues detected by confocal microscopy. scale bar, 100 um. **P < 0.01 vs. gastric cancer tissue. T: tumor; N: non-cancerous tissues. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31801938), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq, Fe, Gp, Gt, Pm, Pm, Rb, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq, Fe, Gp, Gt, Pm, Pm, Rb, Sh

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-33006AF350
Alexa Fluor 405	NBP2-34522AF405
Alexa Fluor 488	NBP2-34522AF488
Alexa Fluor 532	NBP2-33006AF532
Alexa Fluor 594	NBP2-33006AF594
Alexa Fluor 647	NBP2-34522AF647
Alexa Fluor 700	NBP2-34522AF700
Alexa Fluor 750	NBP2-33006AF750
Allophycocyanin	NBP2-34522APC
Biotin	NBP2-34522B
DyLight 350	NBP2-34522UV
DyLight 405	NBP2-34522V
DyLight 488	NBP2-34522G
DyLight 550	NBP2-34522R
DyLight 594	NBP2-33006DL594
DyLight 650	NBP2-34522C
DyLight 680	NBP2-34522FR
DyLight 755	NBP2-34522IR
FITC	NBP2-34522F
HRP	NBP2-34522H
Janelia Fluor 549	NBP2-34522JF549
Janelia Fluor 646	NBP2-34522JF646
PE	NBP2-34522PE
PerCP	NBP2-34522PCP

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-33006-0.2mg
BSA Free	NBP2-33006-0.02mg
Azide and BSA Free	NBP2-34522-0.2mg
Azide and BSA Free	NBP2-34522-0.1mg

compare

(select up to 3 products from this page to compare)

NB600-562

CD31/PECAM-1 Antibody (JC/70A) - BSA Free

(4 Reviews)

(54 Publications)

0.1 ml

NB600-562

CD31/PECAM-1 was detected in immersion fixed THP-1 (red, positive) but not THP-1 KO (negative) cells using 8 ug/mL Mouse Anti-Human CD31/PECAM-1 (JC/70A) Monoclonal Antibody (Catalog # NB600-562) for 3 hours at room temperature. Cells were stained with the NorthernLights(TM) 557-conjugated Donkey anti-Mouse IgG Secondary Antibody (red, Catalog # NL007)) and and counterstained with DAPI (blue).

<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for CD31 mRNA (ACD RNAScope probe, catalog # 487381; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (NB600-562) at 1:25 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).

Rabbit Heart, CD31 Stained Red. Image from verified customer review.

A cell surface stain was performed on HUVEC cells with ( NB600-562, blue) along with a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at RT. (Panel A). A negative control (HeLa cells) was also stained to ensure antibody specificity (Panel B).

GMF-beta is involved in neovasculogenesis in human glioblastoma. A. Co-expression of GMF-beta and CD31/PECAM-1 in several tumor cells. B. The GMF-beta+/CD31+ incomplete microvessel-like structure in hypovascular zones. C. GMF-beta+/CD31+ immature microvessel in hypovascular zones. D. Single GMF-beta+/CD31+ mature microvessel in vascularized areas. E. GMF-beta-/CD31+ mature microvessels in vascularized areas. Red arrows denote GMF-beta staining, black arrows indicate CD31/PECAM-1 staining. Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/abstract/5509), licensed under a CC-BY license.

Analysis of CD31/PECAM1 in human spleen using DAB with hematoxylin counterstain.

Analysis of CD31/PECAM1 expression in 1) Jurkat whole cell lysate, 2) human platelet lysate and 3) U937 whole cell lysate.

Comparison of the expression of NMDAR1 on vessels and EndoMT markers in the urinary bladder.Biopsy specimens of frozen sections from the urinary bladders of (A, C, E) normal control subjects and (B, D, F) KC patients. (A, B) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) and NMDAR1 (red); DAPI was used as a nuclear stain (blue); co-expression of CD31 and NMDAR1 was evident in two groups (yellow, denoted by white arrows). (CF) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) and two mesenchymal markers: FSP1 (red, in panels C and D) and -SMA (red, in panels E and F); DAPI was used as a nuclear stain (blue); co-expression of CD31 and FSP1 or -SMA was evident in three groups (yellow, denoted by white arrows) (white bars: 50 m). Microvascular Injury in Ketamine-Induced Bladder Dysfunction. PLoS One (2016)

A surface stain was performed on Jurkat cells with the Dylight 550 conjugate of CD31/PECAM-1 Antibody (JC/70A) (NB600-562R, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

HUVEC cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CD31/PECAM-1 Antibody (JC/70A) at 2 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Imaging of Feline Endothelial cells with antibody dilution of 1:25. CD31/PECAM-1 (Red), DAPI (Blue). This image was submitted via customer Review.

FFPE human tonsil stained with CD31/PECAM-1 Antibody (JC/70A).

A cell surface stain was performed on Jurkat cells with the Alexa Fluor 488 conjugate of CD31/PECAM-1 Antibody (JC/70A) (NB600-562AF488, blue) along with a matched isotype control. Cells were incubated in an antibody dilution of 10 ug/ml for 20 minutes at RT. Both antibodies were conjugated to Alexa Fluor 488.

A cell surface stain was performed on THP-1 cells with the Alexa Fluor 488 conjugate of CD31/PECAM-1 Antibody (JC/70A) NB600-562AF488 (blue) along with a matched isotype control. Cells were incubated in an antibody dilution of 5 ug/ml for 20 minutes at RT. Both antibodies were conjugated to Alexa Fluor 488.

A surface stain was performed on THP-1 cells with the Alexa Fluor 647 conjugate of CD31/PECAM-1 Antibody (JC/70A) (NB600-562AF647, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) - BSA Free [NB600-562] - Immunohistochemial comparison of the expression of NMDAR1 on vessels & EndoMT markers in the urinary bladder.Biopsy specimens of frozen sections from the urinary bladders of (A, C, E) normal control subjects & (B, D, F) KC patients. (A, B) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & NMDAR1 (red); DAPI was used as a nuclear stain (blue); co-expression of CD31 & NMDAR1 was evident in two groups (yellow, denoted by white arrows). (C–F) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & two mesenchymal markers: FSP1 (red, in panels C & D) & alpha -SMA (red, in panels E & F); DAPI was used as a nuclear stain (blue); co-expression of CD31 & FSP1 or alpha -SMA was evident in three groups (yellow, denoted by white arrows) (white bars: 50 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27529746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

IF staining of IPAH patient CD31/PECAM-1 lung slice. Image from a verified customer review.

Reactivity:

Hu, Mu, Fe, Rb

Species Glossary

Reactivity: Hu, Mu, Fe, Rb

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-562AF350
Alexa Fluor 405	NB600-562AF405
Alexa Fluor 488	NB600-562AF488
Alexa Fluor 532	NB600-562AF532
Alexa Fluor 594	NB600-562AF594
Alexa Fluor 647	NB600-562AF647
Alexa Fluor 700	NB600-562AF700
Alexa Fluor 750	NB600-562AF750
Allophycocyanin	NB600-562APC
Biotin	NB600-562B
CoraFluor 1	NB600-562CL1
DyLight 350	NB600-562UV
DyLight 405	NB600-562V
DyLight 488	NB600-562G
DyLight 550	NB600-562R
DyLight 594	NB600-562DL594
DyLight 650	NB600-562C
DyLight 680	NB600-562FR
DyLight 755	NB600-562IR
FITC	NB600-562F
HRP	NB600-562H
Janelia Fluor 525	NB600-562JF525
Janelia Fluor 549	NB600-562JF549
Janelia Fluor 646	NB600-562JF646
Janelia Fluor 669	NB600-562JF669
PE	NB600-562PE
PerCP	NB600-562PCP
mFluor Violet 450 SE	NB600-562MFV450
mFluor Violet 500 SE	NB600-562MFV500
mFluor Violet 610 SE	NB600-562MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-562SS
Azide and BSA Free	NBP2-80641

compare

(select up to 3 products from this page to compare)

H00010413-M01

YAP1 Antibody (2F12) - Isoform 5 - Azide and BSA Free

(65 Publications)

0.1 mg

H00010413-M01

Immunocytochemistry/Immunofluorescence: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of monoclonal antibody to YAP1 on HeLa cell. Antibody concentration 30 ug/ml.

Immunohistochemistry-Paraffin: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of monoclonal antibody to YAP1 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 3 ug/ml.

Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - JNK specifically phosphorylates YAP in vivo. HaCaT, U2OS, MCF7 and 293T cell lines were treated with anisomycin or DMSO (control) for 60 min before harvesting. YAP band shift was analyzed as described before and lysates were analyzed by Western blotting with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis20107) licensed under a CC-BY license.

Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - Immunogen detection using YAP1 antibody (2F12) Isoform 5, observered MW 37.73KDa) .

Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of YAP1 Antibody (2F12) Isoform 5 in transfected 293T cell line. Lane 1: YAP1 transfected lysate (TMW: 54.5 KDa, observed MW 75 KDa). Lane 2: Non-transfected lysate.

Reactivity:

Hu, Mu, Po, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Po, Bv, Ca

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NB200-209

NQO-1 Antibody (A180) - BSA Free

(57 Publications)

0.1 ml

NB200-209

Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Analysis of NQO1 in human breast cancer using DAB with hematoxylin counterstain.

Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Western Blot: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO1 in A549 lysates using NB 200-209. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.

Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Immunohistochemistry showing Anti-NQ01 on formalin fixed paraffin embedded human kidney tissue

Flow (Intracellular): NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U87MG cells with NQO1 (A180) antibody NBP2-24917 (blue) along with a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

Simple Western: NQO-1 Antibody (A180) [NB200-209] - Simple Western lane view shows a specific band for NQO-1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: NQO-1 Antibody (A180) [NB200-209] - Equol attenuated ER stress by activating Nrf2 in vitro & in vivo.(A) HUVECs were incubated with different concentrations (1, 10 & 100 nM) of equol for 24 h before treatment with t-BHP (50 μM) for another 6 h. The expression of Nrf2 & NQO1 was detected by western blotting. (C) Total tissue lysates from the thoracic & abdominal aorta of apoE-/- mice with or without equol treatment were immunoblotted with anti-Nrf2 & anti-beta -actin antibodies. (E) Cells were transfected with Nrf2 siRNA for 5~6 h; the medium was then replaced with fresh culture medium, followed by incubation for another 24 h. Thereafter, the cells were treated with equol (100 nM) for 24 h & then were incubated with t-BHP (50 μM) for an additional 6 h. The cells were collected & lysed, & western blot analysis was performed. (B)(D)(F) The bar charts show the quantification of the indicated proteins. (G) HUVECs were transfected with Nrf2 siRNA & treated as described in (C), & cell apoptosis was assayed using the Cell Death Detection ELISAplus Kit. Values are presented as means ± SD. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001 versus the control group, RC group or siRNA treated with no drug group; #p < 0.01, ##p < 0.001 versus the t-BHP-treated group & siRNA plus t-BHP treated group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27907038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NQO-1 Antibody (A180) [NB200-209] - S-(-)equol primarily up-regulated Nrf2 protein expression activating ARE-dependent response.(A) Whole EA.hy926 cells lysates of cells treated with 250 nM S-(-)equol, 500 nM daidzein, or 50 µM tBHQ for 24 h were subjected to immunoblot analysis with anti-Nrf2, anti-HO-1, anti-NQO1, & anti-beta -tubulin antibodies. (B) Total HUVECs lysates of cell treated with 250 nM S-(-)equol for 24 h were immunoblotted with anti-Nrf2 & anti-beta -tubulin antibodies. (C) EA.hy926 cells were transfected with plasmids containing an HA-tagged Nrf2 open reading frame & the renilla firefly luciferase gene. Medium was changed after 6 hours of incubation. Where indicated, extracts from HA-Nrf2 EA hy.92 cells were additionally probed with anti-HA antibody, anti-Nrf2, anti-HO-1, anti-NQO1 & anti-beta -tubulin antibodies. Values are means of three independent experiments with standard deviations represented by vertical bars. Mean values were significantly different compared to controls (*p<0.05). Total RNA was extracted from EA.hy926 cells or HUVECs treated as indicated, & Nrf2 (D), HO-1 (E), & NQO1 (F) mRNA was measured by real-time RT-PCR analysis. The values shown represent the mean ± SD obtained for three independent experiments (*p<0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NQO-1 Antibody (A180) [NB200-209] - Phf2 protects the liver from high fat & high sucrose diet-induced fibrogenesis during obesity. GFP or Phf2 were overexpressed through AAV strategy specifically in the liver of 7-week-old male C57Bl/6 J mice. Mice were then fed with either a chow diet (NCD) or with a high fat & high sucrose diet (HFHSD) for 16 weeks. Mice were studied in the fed state. a Representative western blot analysis showing the contribution of Phf2 overexpression to the regulation of the PI3K/Akt signaling & pro-fibrogenic pathways in liver extracts (n = 15 per group). b (Left) Oral glucose tolerance test & insulin levels during the OGTT test. (right) Insulin tolerance test (n = 15 per group). c Liver sections stained with hematoxylin & eosin (H&E), trichrome masson & sirius red are shown. Scale bars = 100 μm (n = 10 per group). d Percentage of fibrotic area & percentage of apoptotic hepatocytes (n = 15 per group). e Relative oxidized GSSG content & measurement of Gpx activity (n = 10 per group). f Levels of carbonylated proteins (n = 10 mice per group). All error bars represent mean ± SEM. Statistical analyses were made using unpaired t-test (b) or Anova, followed by Bonferonni’s test (d, e). *P < 0.01 HFHSD/GFP compared to HFHSD/Phf2, $P < 0.01 HFHSD/GFP compared to NCD/GFP, £P < 0.01 HFHSD/Phf2 compared to HFHSD/GFP Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04361-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: NQO-1 Antibody (A180) [NB200-209] - Activation of Nrf2 signaling pathway by FST in RAW 264.7 mouse macrophage-like cells. Cells were treated with FST with or without 100 ng/mL RANKL for 5 days. (A) Total cellular proteins were isolated from cells & the expression levels of Nrf2 & its regulatory proteins were assessed by Western blot analysis. beta -actin was used as the internal control. (C) The expression of nuclear & cytosol Nrf2 were determined by Western blotting. Lamin B & beta -actin were used as internal controls for the nuclear & cytosolic fractions, respectively. The results shown are representative of three independent experiments. (B,D) Statistical analyses were conducted using analysis of variances between groups. * p < 0.05 & *** p < 0.0001 when compared to control. # p < 0.05 & ### p < 0.0001 when compared to RANKL treatment. RANKL: receptor of activator of nuclear factor kappa-B ligand; FST: fermented sea tangle extract; Nrf2: nuclear factor-erythroid 2-related factor 2; p-Nrf2: phosphorylated nuclear factor-erythroid 2-related factor 2; HO-1: heme oxygenase-1; NQO-1: NAD(P)H quinone oxidoreductase 1; +: cells treated the reagent; -: cells untreated the reagent. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31357503), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: NQO-1 Antibody (A180) [NB200-209] - Nrf2-pathway activation by hybrids: nuclear translocation & targets induction. (A) Nuclear cellular extracts of SH-SY5Y cells were treated for 3 hours with compounds at 5 µM, 500 nM, & 50 nM or with 20, 10, & 5 µM dimethyl fumarate (DMF). Nrf2 protein content in the nucleus was determined by Western blot. Anti-lamin A/C was used as a protein loading control. Results are shown as ratio Nrf2/lamin A/C ± SEM; *p < 0.05, **p < 0.01 & ****p < 0.0001 versus CTR; Dunnett’s multiple comparison test (F ratio = 6.797, n≥3). (B–C) RNA from total cellular extracts of SH-SY5Y cells, treated for 24 hours with 5 μM compounds or 20 μM DMF, were analyzed for NQO1 (B) & HO-1 (C) mRNA expression by RT-qPCR. GAPDH was used as housekeeping gene. Results are shown as mean ± SEM; *p < 0.05, **p < 0.01, & ***p < 0.001 versus CTR; Dunnett’s multiple comparison test (B, n≥3, F ratio = 10.44; C, n≥3, F ratio = 13.95). (D–E) Cellular extracts of SH-SY5Y cells treated for 24 hours with compounds at 5 μM or 20 μM DMF were analyzed for NQO1 (D) & HO-1 (E) protein levels by Western blot. Anti-actin was used as protein loading control. Results are shown as ratio (% of CTR) ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, & ****p < 0.0001 versus CTR; Dunnett’s multiple comparison test (D, n ≥ 3, F ratio = 5.144; E, n≥3, F ratio = 17.26). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32047434), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NQO-1 Antibody (A180) [NB200-209] - The effects of PI3K/Akt & ER inhibitors on S-(-)equol-induced Nrf2 activation in endothelial cells.(A) EA.hy926 cells or HUVECs transfected w/ ARE-dependent firefly luciferase reporter gene (F) & the renilla firefly luciferase gene (R), & treated w/ 250 nM S-(-)equol in the presence or absence of 10 µM LY294002 or 100 nM ICI182,780 for 16 h. The potency of induction is expressed as the relative luminescence (R/F) measured using the dual luciferase reporter assay system (mean ± SD, n = 3). *p<0.05 versus w/ control group, #p<0.05 versus w/ S-(-)equol treated group. (B) EA.hy926 cells or HUVECs incubated w/ or w/out 10 µM LY294002 or 100 nM ICI182,780 for 30 min & then w/ or w/out 250 nM S-(-)equol or 500 nM daidzein for 24 h. Whole cell lysates then immunoblotted w/ antibodies against Nrf2, HO-1, NQO1, & beta -tubulin. Values are means of three independent experiments w/ standard deviations represented by vertical bars. Mean values significantly different compared w/ controls (*p<0.05). (C) EA.hy926 cells or HUVECs cotransfected w/ the HA-Nrf2 & renilla firefly luciferase expression plasmids treated w/ 250 nM S-(-)equol in the presence or absence of 10 µM LY294002 or 100 nM ICI182,780 for 16 h, & HA-Nrf2 localization analyzed by confocal microscopy. (D) EA.hy926 cells incubated w/ 250 nM S-(-)equol, 500 nM daidzein w/ or w/out 10 µM LY294002 or 100 nM ICI182,780 for 16 h & then immunostained w/ an antibody against Nrf2, & Nrf2 localization analyzed by confocal microscopy. Values (intensity of nuclear versus cytoplasmic) are means of counting 100 cells w/ standard deviations represented by vertical bars. *p<0.05 versus w/ control group, #p<0.05 versus w/ S-(-)equol treated group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: NQO-1 Antibody (A180) [NB200-209] - Activation of Nrf2 signaling pathway by FST in RAW 264.7 mouse macrophage-like cells. Cells were treated with FST with or without 100 ng/mL RANKL for 5 days. (A) Total cellular proteins were isolated from cells & the expression levels of Nrf2 & its regulatory proteins were assessed by Western blot analysis. beta -actin was used as the internal control. (C) The expression of nuclear & cytosol Nrf2 were determined by Western blotting. Lamin B & beta -actin were used as internal controls for the nuclear & cytosolic fractions, respectively. The results shown are representative of three independent experiments. (B,D) Statistical analyses were conducted using analysis of variances between groups. * p < 0.05 & *** p < 0.0001 when compared to control. # p < 0.05 & ### p < 0.0001 when compared to RANKL treatment. RANKL: receptor of activator of nuclear factor kappa-B ligand; FST: fermented sea tangle extract; Nrf2: nuclear factor-erythroid 2-related factor 2; p-Nrf2: phosphorylated nuclear factor-erythroid 2-related factor 2; HO-1: heme oxygenase-1; NQO-1: NAD(P)H quinone oxidoreductase 1; +: cells treated the reagent; -: cells untreated the reagent. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31357503), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

NQO-1 (A180) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-NQO-1 (A180) Protein G Purified Monoclonal Antibody conjugated to DyLight 650 (Catalog # NB200-209C) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Rt, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB200-209AF350
Alexa Fluor 405	NB200-209AF405
Alexa Fluor 488	NB200-209AF488
Alexa Fluor 532	NB200-209AF532
Alexa Fluor 594	NB200-209AF594
Alexa Fluor 647	NB200-209AF647
Alexa Fluor 700	NB200-209AF700
Alexa Fluor 750	NB200-209AF750
Allophycocyanin	NB200-209APC
Biotin	NB200-209B
CoraFluor 1	NB200-209CL1
DyLight 350	NB200-209UV
DyLight 405	NB200-209V
DyLight 488	NB200-209G
DyLight 550	NB200-209R
DyLight 594	NB200-209DL594
DyLight 650	NB200-209C
DyLight 680	NB200-209FR
DyLight 755	NB200-209IR
FITC	NB200-209F
HRP	NB200-209H
Janelia Fluor 525	NB200-209JF525
Janelia Fluor 549	NB200-209JF549
Janelia Fluor 646	NB200-209JF646
Janelia Fluor 669	NB200-209JF669
PE	NB200-209PE
PerCP	NB200-209PCP
mFluor Violet 450 SE	NB200-209MFV450
mFluor Violet 500 SE	NB200-209MFV500
mFluor Violet 610 SE	NB200-209MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB200-209SS
Azide and BSA Free	NBP2-80885

compare

(select up to 3 products from this page to compare)

NBP1-19371

CD4 Antibody - BSA Free

(9 Reviews)

(53 Publications)

0.1 ml

NBP1-19371

Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.

Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).

Flow Cytometry: CD4 Antibody - BSA Free [NBP1-19371] - An intracellular stain was performed on U-251 MG cells with CD4 Antibody NBP1-19371AF594 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Western Blot: CD4 Antibody [NBP1-19371] - Mouse spleen, 25ug total protein. Image from verified customer review.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Staining of paraffin-embedded mouse brain tissue. Photo courtesy of product review by verified customer.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Analysis of a FFPE tissue section of mouse brain using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody primarily generated a strong signal in the membranes of a subset of cells in the tested section.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Analysis of a FFPE section of mouse spleen using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong signal in the membranes of a subset of cells in the spleen section and the staining showed punctate appearance in some cells.

Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on U937 cells with CD4 Antibody NBP1-19371AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on U937 cells with CD4 Antibody NBP1-19371G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.

Simple Western: CD4 Antibody [NBP1-19371] - Lane view shows a specific band for CD4 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Dual RNAscope ISH-IHC: CD4 Antibody [NBP1-19371] - IFNG mRNA (red) and CD4 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human lung cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-IFNG and CD4 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin (Cat. No. DS9800), BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).

Dual RNAscope ISH-IHC: CD4 Antibody [NBP1-19371] - CCR5 mRNA (red) and CD4 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human lung cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CCR5 and CD4 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).

ATM activation by chloroquine alleviates senescence.(a) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in human skin fibroblasts (HSFs). A gradually increased level of p16 indicates cellular senescence, while elevated gamma H2AX level indicates accumulated DNA damage. (b) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in mouse embryonic fibroblasts (MEFs). (c) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in brain tissues isolated from 3-, 10-, and 18-month-old male mice. (d) SA-beta -Gal staining in HSFs treated with sh-ATM or scramble shRNA. Scale bar, 100 µm. (e) Quantification of SA-beta -Gal-positive staining of (d) from five views randomly captured for each group. Data represent means ± SEM. ***p<0.001. (f) Immunoblots showing increased gamma H2AX and unaffected LC3I/II in HSFs treated with sh-ATM or scramble shRNA. (g) Immunoblots showing protein levels of pS1981 ATM, gamma H2AX, and cleaved caspase-3 in HSFs treated with 10 μM of CQ for indicated time. (h) SA-beta -Gal staining in HSFs expressing either scramble or ATM shRNA treated with 1 μM CQ or DMSO (12 hr). Scale bar, 100 µm. (i) Quantification of SA-beta -Gal-positive staining of (h) from five views randomly captured for each group. Data represent means ± SEM. ***p<0.001; ‘N.S.’ indicates no significant difference. (j) HSFs at passage 20 were continuously cultured with 1 μM CQ or DMSO, and cell number was calculated at each passage. Data represent means ± SEM. ***p<0.01. (k) Immunoblots showing protein levels of gamma H2AX, p62, and LC3 in MEFs treated with 1 μM CQ or DMSO. Note that CQ had little effect on the expression levels of p62 and LC3. (l) MEFs at passage one were continuously cultured in 20% O2 with 1 μM CQ or DMSO, and cell number was determined at each passage. Data represent means ± SEM. ***p<0.01.10.7554/eLife.34836.006Figure 1—source data 1.Statistical analysis for SA-beta -Gal positive staining.10.7554/eLife.34836.007Figure 1—source data 2.Statistical analysis for EdU positive staining.Statistical analysis for SA-beta -Gal positive staining.Statistical analysis for EdU positive staining.Decline of ATM-centered DNA repair machinery during senescence.(a) Real-time PCR analysis showing progressively elevated mRNA level of p21 in continuously cultured human endothelial cells (HUVEC). **p<0.01. (b) SA-beta -Gal staining of HUVEC cells at indicated passages. Scale bar, 100 µm. (c) HUVEC cells at P21, P18, P12, and P7 were subjected to transcriptome analysis. A minimum average rpkm value of 1.0 and maximum 10% fluctuation in young cells (P7 Vs P12) was set as the threshold. Genes were downregulated by more than 20% in pre-senescent, and senescent cells compared with young cells (P21/P18 Vs P12/P7) were selected. (d) Pathway analysis of genes identified in (c) by STRING v10. (e) Downregulation of ATM-related DNA repair genes during senescence.ATM regulates replicative senescence.(a) Representative images showing cells treated with Scramble (sh-NC) or sh-ATM. (b) Percent EdU-positive cells in sh-NC or sh-ATM treated HSFs. Views were randomly captured and at least 100 cells were included in each group. Data represent means ± SEM. ***p<0.001. (c) Immunoblots showing protein levels of pS1981 ATM and gamma H2AX in HSFs treated with 10 μM chloroquine (CQ) or 0.4 μM CPT (4 hr). Note that CQ activated ATM (pS1981) without increasing gamma H2AX, while CPT activated ATM accompanied by increased gamma H2AX. (d) SA-beta -Gal staining in primary MEFs treated with 1 μM CQ or DMSO. Scale bar, 100 µm. (e) Quantification of SA-beta -Gal-positive staining of (d) from five views randomly captured for each group. Data represent means ± SEM. ***p<0.001. (f) Percent EdU-positive cells in HSFs treated with DMSO, 1 μM or 10 μM CQ. Views were randomly captured and at least 100 cells were included in each group. Data represent means ± SEM. ***p<0.001. (g) Representative images showing proliferative HSFs treated with different doses of CQ for the indicated time points. (h) Immunoblots showing LC3B levels in HSFs treated with indicated dose of CQ for indicated period of time. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29717979), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CD4 Antibody - BSA Free [NBP1-19371] - Rats with mild liver damage show immune cells infiltration in cerebellum at 4 weeks, which is prevented by rifaximin. Analysis of immune cells infiltration in cerebellum was performed at 4 weeks by immunohistochemistry using antibodies against (A) CD4, a marker of T lymphocytes (F(3,8) = 4.511, p < 0.01) & (B) IBA1, a marker of meningeal & perivascular macrophages (K-W(4,10) = 27.76, p < 0.0001). (C) Double immunofluorescence with anti-CD4 & anti-CX3CR1 as markers of autoreactive CD4+CD28− T lymphocytes was performed & quantified (K-W(4,5) = 8.711, p < 0.05). (D) Double immunofluorescence with anti-CD4 & anti-CCR6 as marker of Th17 lymphocytes was performed & quantified (K-W(4,7) = 8.858, p < 0.05). Number of animals in each group was added under group names. One-way ANOVA with Tukey’s test (CD4+) & nonparametric Kruskal–Wallis (K-W statistic) with Dunn’s test (Iba1, CD4+/CX3CR1+ & CD4+/CCR6+) was performed to compare all groups. Values significantly different from control rats are indicated by asterisks & from CCl4 rats by a. * p < 0.05, a p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Topical 2% REB decreases the ocular inflammatory cytokines in PM-induced ocular surface inflammation in a rat model. (A) Immunofluorescence staining for (i) MMP-9, (ii) TNF-alpha , (iii) IL-1 beta , and (iv) IL-17 in the cornea of normal, PM-exposed, PM+REB, and PM+HA-treated groups. (B) Western blot analysis of pNF-kappa B and NF-kappa B, (C) IL-17, (D) CD-4, and (E) MMP-9 expression in the conjunctiva. (F) Changes in the expression of MMP-9, (G) TNF-alpha , (H) IL-1 beta , and (I) IL-17 in the cornea. (J) Relative expression of pNF-kappa B, (K) IL-17, (L) CD-4, and (M) MMP-9 normalized to GAPDH. In (F–M), the data are expressed as mean +/- SEM (n = 6, n = 4). One-way ANOVA was followed by the Tukey test. * p < 0.05, ** p < 0.01, *** p < 0.001 indicate statistically significant differences. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40332777), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immune signaling in the WAT during CoV-2 infection is altered differently between the sexes. Immunoblot images of (a) immune cell markers (CD4, CD8, F4/80) and (b) inflammatory markers (TNF alpha , IL-6, IL-10). beta -actin was used as loading control. Fold changes in the protein levels were normalized to beta -actin expression and are shown as bar graphs. The error bars represent standard error of the mean. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared to uninfected sex-matched mice and between the infected groups (n = 4/sex/group) (M, male; F, female). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36674830), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Rats with mild liver damage show immune cells infiltration in cerebellum at 4 weeks, which is prevented by rifaximin. Analysis of immune cells infiltration in cerebellum was performed at 4 weeks by immunohistochemistry using antibodies against (A) CD4, a marker of T lymphocytes (F(3,8) = 4.511, p < 0.01) and (B) IBA1, a marker of meningeal and perivascular macrophages (K-W(4,10) = 27.76, p < 0.0001). (C) Double immunofluorescence with anti-CD4 and anti-CX3CR1 as markers of autoreactive CD4+CD28− T lymphocytes was performed and quantified (K-W(4,5) = 8.711, p < 0.05). (D) Double immunofluorescence with anti-CD4 and anti-CCR6 as marker of Th17 lymphocytes was performed and quantified (K-W(4,7) = 8.858, p < 0.05). Number of animals in each group was added under group names. One-way ANOVA with Tukey’s test (CD4+) and nonparametric Kruskal–Wallis (K-W statistic) with Dunn’s test (Iba1, CD4+/CX3CR1+ and CD4+/CCR6+) was performed to compare all groups. Values significantly different from control rats are indicated by asterisks and from CCl4 rats by a. * p < 0.05, a p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

(A) Immunofluorescence staining showing the expression of (i) PD-L1, (ii) CD4, (iii) CD3e and (iv) IL-17 localized in the corneas in the control, desiccation+PBS and desiccation+PD-L1 group. (B–E) Changes in the percentage of PD-L1, CD4, CD3e and IL-17 positive cells in the cornea. Immunopositivity for PD-L1 is shown in higher-power fields. Immunopositivity was counted in low-power fields and calculated as relative to the total number of DAPI-positive cells. (F,G) Western blot showing the expression of CD4 and IL-17 in the cornea. (H,I) Relative expression ratio of CD4 and IL-17 to beta -actin. In (B–E,H,I), data are presented with bar graph (n = 6, day 10, * p < 0.05; ** p < 0.01; *** p < 0.001 significant difference by one-way ANOVA). Original images of (F,G) can be found in Supplementary Materials. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/2218-273X/14/1/68), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

RD and HFD differently regulate the expression levels of p-AMPK alpha , p-mTOR, p-Perilipin 1, CD4, CD8, F4/80, TNF alpha , IFN gamma , and IL-6 in tumor-associated mammary fat pad during early (1 wpi) and later (5 wpi) time points of tumor growth in young female E0771 tumor-bearing mice. (A) Immunoblots showing different protein expression between RD and HFD at 1 wpi and 5 wpi. beta -Actin was used as loading control. (B) Bar graphs showing the levels of each protein marker normalized to beta -Actin (top panel—1 wpi; bottom panel—5 wpi). The error bars represent the standard error of the mean. * p ≤ 0.05 and *** p ≤ 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36675341), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Ca, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ca, Rb

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-19371AF350
Alexa Fluor 405	NBP1-19371AF405
Alexa Fluor 488	NBP1-19371AF488
Alexa Fluor 532	NBP1-19371AF532
Alexa Fluor 594	NBP1-19371AF594
Alexa Fluor 647	NBP1-19371AF647
Alexa Fluor 700	NBP1-19371AF700
Alexa Fluor 750	NBP1-19371AF750
Allophycocyanin	NBP1-19371APC
Biotin	NBP1-19371B
CoraFluor 1	NBP1-19371CL1
DyLight 350	NBP1-19371UV
DyLight 405	NBP1-19371V
DyLight 488	NBP1-19371G
DyLight 550	NBP1-19371R
DyLight 594	NBP1-19371DL594
DyLight 650	NBP1-19371C
DyLight 680	NBP1-19371FR
DyLight 755	NBP1-19371IR
FITC	NBP1-19371F
HRP	NBP1-19371H
Janelia Fluor 525	NBP1-19371JF525
Janelia Fluor 549	NBP1-19371JF549
Janelia Fluor 646	NBP1-19371JF646
Janelia Fluor 669	NBP1-19371JF669
PE	NBP1-19371PE
PerCP	NBP1-19371PCP
mFluor Violet 450 SE	NBP1-19371MFV450
mFluor Violet 500 SE	NBP1-19371MFV500
mFluor Violet 610 SE	NBP1-19371MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-19371SS

compare

(select up to 3 products from this page to compare)

DDX0390P-100

BST2 Antibody (120G8.04) - Azide and BSA Free

(66 Publications)

0.1 mg

DDX0390P-100

Flow Cytometry: BST2 Antibody (120G8.04) [DDX0390P-100] - In vivo depletion of mouse PDCs (gated on CD11c+CD3- cells).

Flow Cytometry: BST2 Antibody (120G8.04) [DDX0390P-100] - Facs sorting of mouse PDCs (120G8/CD11c)

Treatment with HP06T07 induces accumulation of T cells, NK cells, and pDCs. Three mg/kg HP06T07 or PBS was intratumorally injected into right sides of tumors at 50 μl and was administered consecutively six times at 3-day intervals (day 10, 13, 16, 19, 22, and 25; I3). Two days after last treatment, tumors on the right sides of mice were harvested, embedded with paraffin, and stained with anti-mouse CD3ϵ (A), NK cell marker NCR1 (B), pDCs marker PDCA-1 (C), and CD19 (D). Positive cells were detected with alkaline phosphatase-conjugated goat anti-rat/rabbit IgG according to the manufacturer’s instructions. Representative images from one of three HP06T07- or PBS-treated mice are shown. Arrows indicate infiltration of immune cells. Original magnification, 200× and 400×. Histogram results were expressed as mean +/- SEM of positive cells/field evaluated at 400× images (n = 4–6/group). Statistical significance of differences was determined (*P < 0.05 and ***P < 0.001). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32116691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	DDX0390A488-100
Alexa Fluor 546	DDX0390A546-100
Alexa Fluor 647	DDX0390A647-100
Biotin	DDX0390B-100

compare

(select up to 3 products from this page to compare)

NB300-327

GAPDH Antibody

(3 Reviews)

(57 Publications)

0.1 ml

NB300-327

Western Blot: GAPDH Antibody [NB300-327] - Theoretical molecular weight: 36 kDa. Detection of GAPDH in mouse liver.

Western Blot: GAPDH Antibody [NB300-327] - Western blot analysis of extracts from Jurkat and HeLa cells using NB300-327 at 1:1000. Theoretical molecular weight: 36 kDa.

$Western Blot: GAPDH Antibody [NB300-327] - Analysis of different cell cytosolic or nuclear enriched fractions, GAPDH antibody, dilution 1:20,000 (Red): [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions. The same blot was simultaneously probed with mouse mAb to the nuclear RNA binding protein SF3B4, dilution 1:1,000 (Green). In contrast to GAPDH, SF3B4 is exclusively expressed in the nuclear fraction.$

Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-327] - The GAPDH antibody was tested in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.

Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-327] - Confocal immunofluorescence analysis of HeLa cells using GAPDH (NB300-327) antibody (green). Nuclei was counterstained with DAPI (blue).

Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-327] - IF Confocal analysis of C2C12 cells using GAPDH antibody (NB300-327, 1:20). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Simple Western: GAPDH Antibody [NB300-327] - Simple Western lane view shows a specific band for GAPDH in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higer than predicted 36 kDa molecular weight.

$Western Blot: GAPDH Antibody [NB300-327] - Effects of subchronic administration (7 and 14 days) of therapeutic relevant concentration of Brex (Brex: 300 nM) and interaction between Brex and 5-HT1A receptor (5-HT1AR) antagonist WAY (10 uM) on protein expression of 5-HT1A receptor in the plasma membrane fraction of cortical primary cultured astrocytes. In left side histograms, ordinate: mean +/- SD (n = 6) of the relative protein level of 5-HT1AR per GAPDH. * p < 0.05, ** p < 0.01: relative to control (Brex-free) by one-way analysis of variance (ANOVA) with Tukey’s post-hoc test, and @ p < 0.05: relative to Brex for 14 days by Student’s T-test. Right side panels indicate their pseudo-gel images using capillary immunoblotting. Antibodies used: 5-HT1A (NBP2-21590) and GAPDH (NB300-327). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35743014/) licensed under a CC-BY license.$

Representative immunoblot of KDR and FGFR CAM protein expression levels examined by Western blotting. GNS, graphene nanosheet; NG, graphite nanoparticle; ND, diamond nanoparticle; C60, fullerene C60; MWNT, multi-wall nanotube; KDR, vascular endothelial growth factor receptor; FGFR, fibroblast growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Western Blot: GAPDH Antibody [NB300-327] - Increased PIP expression in the lungs of PF‐PH compared to PF ratsA, BRelative expression of PIP mRNA (A) & protein (B) normalized to GAPDH.CRepresentative images & PIP quantification.Data information: Values are expressed as mean ± SEM. The number of samples per group for each experiment is included within each bar graph (t‐test; *P < 0.05, **P < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31468711), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: GAPDH Antibody [NB300-327] - Effects of subchronic administration (7 & 14 days) of therapeutic relevant concentration of Brex (Brex: 300 nM) & interaction between Brex & 5-HT1A receptor (5-HT1AR) antagonist WAY100635 (WAY: 10 μM) on protein expression of 5-HT1A (panel A) & 5-HT7 (panel B) receptor in the plasma membrane fraction of cortical primary cultured astrocytes. In left side histograms, ordinate: mean ± SD (n = 6) of the relative protein level of 5-HT1AR & 5-HT7R per GAPDH. * p < 0.05, ** p < 0.01: relative to control (Brex-free) by one-way analysis of variance (ANOVA) with Tukey’s post-hoc test, & @ p < 0.05: relative to Brex for 14 days by Student’s T-test. Right side panels indicate their pseudo-gel images using capillary immunoblotting. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35743014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: GAPDH Antibody [NB300-327] - Online lung microarray analysis revealed PIP as one of the Slug transcriptional targets mediating vascular cell proliferation in PF‐PHAHeatmap of the 20 genes upregulated in GSE24988 microarray & known to be Slug transcriptional target.BVenn diagram showing the overlap between Slug targets implicated in cell proliferation & known to be extracellular.CHeatmap of the seven transcriptional targets of Slug known to promote proliferation & to be extracellular.D, ERelative expression of prolactin‐induced protein (PIP) mRNA (D) & protein (E) expression normalized to GAPDH in PF & PF‐PH patients.FRepresentative images of PIP immunohistochemistry showing its extracellular localization & quantification.G–JProliferation assay on healthy fibroblast (G), pulmonary arterial EC (H), & healthy pulmonary arterial SMC (I & J) in the presence or absence of PIP.Data information: Values are expressed as mean ± SEM. The number of samples per group for each experiment is included within each bar graph. Statistical test: panels (D–F): t‐test; panels (G–J): ANOVA (*P < 0.05, **P < 0.01 ***P < 0.001). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31468711), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-327] - Growth of lactobacilli on IECs & their influence towards C. albicans cytotoxicity, adhesion, hyphal length & translocation. (A) LDH release of IECs colonized (Pre-Inc.) or simultaneously colonized (w/o Pre-Inc.) with L. rhamnosus (L.r.) at different MOI (5, 50 or 250) & infected or not with C. albicans (C.a.) (MOI 1) & measured at 24 h post-infection. (B) Growth of L. paracasei (L.p.), L. rhamnosus, L. salivarius (L.s.), L. fermentum (L.f.), & L. brevis (L.b.) on IECs. (C) Percentage of C. albicans adhered to IECs colonized with different Lactobacillus species (MOI 50) at 1 h post-infection. (D) C. albicans hyphal induction on IECs or on plastic colonized with L. rhamnosus or L. brevis (MOI 50) at 4 h post-infection. Results were normalized to C. albicans single infection. (E) Translocation of C. albicans (MOI 1) across IECs colonized with L. rhamnosus or L. brevis (MOI 50) at 24 h post-infection. (F) Assessment of epithelial barrier integrity measured as the loss of transepithelial electrical resistance (TEER) in response to L. rhamnosus or L. brevis (MOI 50) colonization & C. albicans infection in the presence or absence of Lactobacillus colonization at 24 h post-infection. Data are TEER loss in percentage of uninfected host cells (before pre-incubation). (G) E-Cadherin protein expression analyzed by western blot compared to GAPDH in IECs that were left uninfected or colonized with L. rhamnosus (MOI 50) & infected with C. albicans for 6, 12 & 24 h. Data are mean±s.e.m. *P<0.05, **P<0.01, ***P<0.005 (t-test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31413153), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Panel (D) indicates the expression of connexin43 (Cx43) and pannexin1 (PANX1) in the plasma membrane fraction of the PPN between wild type (grey columns) and S286L-TG (black columns). Ordinates indicate the mean � SD (n = 6) of the relative expression of connexin43 and pannexin1 per GAPDH. Right-side panels indicate the pseudo-gel images of connexin43, pannexin1 and GAPDH, obtained using capillary immunoblotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/40564986/), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt, Po, Ba, Bv, Ch, Eq, Fu, In, Ye

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ba, Bv, Ch, Eq, Fu, In, Ye

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NB100-64796

CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free

(1 Review)

(61 Publications)

0.25 mg

NB100-64796

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (TLD-3A12) [NB100-64796] - Analysis of CD31 in 4th day cutaneous wound section of diabetic rat using CD31/PECAM-1 Antibody (TLD-3A12). Image from verified customer review.

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (TLD-3A12) [NB100-64796] - IHC analysis for the expression of CD31 of wound tissues of different groups of rats (magnification 200). n = 6, **P less than 0.01. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/sci/2019/9564869/) licensed under a CC-BY license.

(a) HE staining for wound tissues of different groups of rats (magnification ×40). (b) IHC analysis for the expression of CD31 of wound tissues of different groups of rats (magnification ×200). n = 6, ∗∗P < 0.01.

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-64796AF350
Alexa Fluor 405	NB100-64796AF405
Alexa Fluor 488	NB100-64796AF488
Alexa Fluor 532	NB100-64796AF532
Alexa Fluor 594	NB100-64796AF594
Alexa Fluor 647	NB100-64796AF647
Alexa Fluor 700	NB100-64796AF700
Alexa Fluor 750	NB100-64796AF750
Allophycocyanin	NB100-64796APC
Biotin	NB100-63701
Biotin	NB100-64796B
CoraFluor 1	NB100-64796CL1
DyLight 350	NB100-64796UV
DyLight 405	NB100-64796V
DyLight 488	NB100-64796G
DyLight 550	NB100-64796R
DyLight 594	NB100-64796DL594
DyLight 650	NB100-64796C
DyLight 680	NB100-64796FR
DyLight 755	NB100-64796IR
FITC	NB100-63703-100ug
FITC	NB100-64796F
HRP	NB100-64796H
Janelia Fluor 525	NB100-64796JF525
Janelia Fluor 549	NB100-64796JF549
Janelia Fluor 646	NB100-64796JF646
Janelia Fluor 669	NB100-64796JF669
PE	NB100-64796PE
PerCP	NB100-64796PCP
mFluor Violet 450 SE	NB100-64796MFV450
mFluor Violet 500 SE	NB100-64796MFV500
mFluor Violet 610 SE	NB100-64796MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-64796SS

compare

(select up to 3 products from this page to compare)

NB110-55288

DRP1 Antibody - BSA Free

(3 Reviews)

(49 Publications)

0.1 ml

NB110-55288

<b>Genetic Strategies Validation. </b>Western Blot: DRP1 Antibody [NB110-55288] - Lane 1: DRP1 knockout. Lane 2: DRP1 wildtype MEFs. Stained with NB110-55288 at 1:500.

Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-DRP1 at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: DRP1 Antibody [NB110-55288] - Staining of renal tubular epithelium and visceral epithelial cells of the glomerulus. Human kidney cortex, 40X magnification.

Western Blot: DRP1 Antibody [NB110-55288] - Assessment of skeletal muscle mitochondrial dynamic markers. Representative western blots. Significant main effect of training: #, p < 0.05; ##, p < 0.01. Significant post-hoc training effect: *, p < 0.05; **, p < 0.01. Data are presented as mean +/- SEM. Image collected and cropped by CiteAb from the following publication (null), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DRP1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: DRP1 Antibody [NB110-55288] - An intracellular stain was performed on HeLa cells with DRP1 Antibody NB110-55288AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Western Blot: DRP1 Antibody [NB110-55288] - Rat spinal cord-DRP1 (81kda). Image from verified customer review.

Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DRP1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: DRP1 Antibody [NB110-55288] - An intracellular stain was performed on HeLa cells with NB110-55288 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.

Simple Western: DRP1 Antibody [NB110-55288] - Image shows a specific band for DUX4 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: DRP1 Antibody - BSA Free [NB110-55288] - Image shows a specific band for DRP1 in 0.1 ug/uL mouse hippocampus tissue lysate. Primary antibody dilution: 1:200. Image from verified customer review.

Assessment of skeletal muscle mitochondrial dynamic markers. (A) Total Drp1 protein content, (B) phospho-Drp1Ser616 protein content, (C) percent difference in total and phospho-Drp1Ser616 in ET participants relative to SED participants, (D) total Mfn1 protein content, (E) total Mfn2 protein content. (F) Representative western blots. Significant main effect of training: #, p < 0.05; ##, p < 0.01. Significant post-hoc training effect: *, p < 0.05; **, p < 0.01. Data are presented as mean ± SEM.

An intracellular stain was performed on SJCRH30 human Rhabdomyosarcoma cell line with Rabbit anti-DRP1 Affinity-purified Polyclonal Antibody conjugated to FITC (Catalog # NB110-55288F, blue histogram) or matched control antibody (NBP2-24892, orange histogram) at 5 µg/mL for 30 minutes at RT.

Reactivity:

Hu, Mu, Rt, Fi, Pm, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Fi, Pm, Bv

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-55288AF350
Alexa Fluor 405	NB110-55288AF405
Alexa Fluor 488	NB110-55288AF488
Alexa Fluor 532	NB110-55288AF532
Alexa Fluor 594	NB110-55288AF594
Alexa Fluor 647	NB110-55288AF647
Alexa Fluor 700	NB110-55288AF700
Alexa Fluor 750	NB110-55288AF750
Allophycocyanin	NB110-55288APC
Biotin	NB110-55288B
CoraFluor 1	NB110-55288CL1
DyLight 350	NB110-55288UV
DyLight 405	NB110-55288V
DyLight 488	NB110-55288G
DyLight 550	NB110-55288R
DyLight 594	NB110-55288DL594
DyLight 650	NB110-55288C
DyLight 680	NB110-55288FR
DyLight 755	NB110-55288IR
FITC	NB110-55288F
HRP	NB110-55288H
Janelia Fluor 525	NB110-55288JF525
Janelia Fluor 549	NB110-55288JF549
Janelia Fluor 646	NB110-55288JF646
Janelia Fluor 669	NB110-55288JF669
PE	NB110-55288PE
PerCP	NB110-55288PCP
mFluor Violet 450 SE	NB110-55288MFV450
mFluor Violet 500 SE	NB110-55288MFV500
mFluor Violet 610 SE	NB110-55288MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-55288SS

compare

(select up to 3 products from this page to compare)

NB100-77417

CD45 Antibody (30-F11) - BSA Free

(5 Reviews)

(48 Publications)

0.5 mg

NB100-77417

Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Mouse glomeruli stained with CD45 antibody. 4% formaldehyde fixed paraffin sections. EDTA was used for antigen retrieval. IHC-P image submitted by a verified customer review.

Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a FFPE embedded tissue section of mouse liver using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. The signal was developed using AEC reagent based method with hematoxylin counterstaining.

Immunohistochemistry-Frozen: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a mouse spleen cryosection using CD45 antibody (30-F11) [NB100-77417] at 1:50 dilution. The signal was detected using goat anti-rat Alexa fluor 488 secondary with DAPI counterstaining. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Staining of a FFPE mouse liver tissue section using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. Signal was detected using AF488 conjugated donkey anti-rat secondary antibody at 1:500 dilution. IHC-P image submitted by a verified customer review.

Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Severe apoptosis and pathology in the orally infected heart. Experimental design is as outlined in Fig. 1B. Heart sections from moribund mice were examined with H&E, and immunohistochemistry staining for pan-leukocyte infiltration using an anti-CD45 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.

Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - VP1-positive infiltrating M2 macrophage and VP1-positive pneumocytes were detectable in the orally infected lung. Adjacent lung sections were examined with H&E and IHC staining for a pan-leukocyte marker CD45. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-77417AF350
Alexa Fluor 405	NB100-77417AF405
Alexa Fluor 488	NB100-77417AF488
Alexa Fluor 532	NB100-77417AF532
Alexa Fluor 594	NB100-77417AF594
Alexa Fluor 647	NB100-77417AF647
Alexa Fluor 700	NB100-77417AF700
Alexa Fluor 750	NB100-77417AF750
Allophycocyanin	NB100-77417APC
Biotin	NB100-77417B
CoraFluor 1	NB100-77417CL1
DyLight 350	NB100-77417UV
DyLight 405	NB100-77417V
DyLight 488	NB100-77417G
DyLight 550	NB100-77417R
DyLight 594	NB100-77417DL594
DyLight 650	NB100-77417C
DyLight 680	NB100-77417FR
DyLight 755	NB100-77417IR
FITC	NB100-77417F
HRP	NB100-77417H
Janelia Fluor 525	NB100-77417JF525
Janelia Fluor 549	NB100-77417JF549
Janelia Fluor 646	NB100-77417JF646
Janelia Fluor 669	NB100-77417JF669
PE	NB100-77417PE
PerCP	NB100-77417PCP
mFluor Violet 450 SE	NB100-77417MFV450
mFluor Violet 500 SE	NB100-77417MFV500
mFluor Violet 610 SE	NB100-77417MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80652-0.1mg
BSA Free	NB100-77417SS
Azide and BSA Free	NBP2-80652

compare

(select up to 3 products from this page to compare)

NBP2-24917

TLR8 Antibody (44C143) - BSA Free

(52 Publications)

0.1 mg

NBP2-24917

<b>Orthogonal Strategies Validation. </b>Western Blot: TLR8 Antibody (44C143) [NBP2-24917] - All the cell lines expressed mRNA for TLR8 (Figure 1) and displayed a strong expression of TLR8 protein (Figure 2). Lysates of human intestinal tissue and mouse splenocytes were used as positive controls. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: TLR8 Antibody (44C143) [NBP2-24917] - Formalin-fixed, paraffin-embedded human spleen stained with TLR8 antibody at 2 ug/ml.

Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - HEK cells expressing TLR8 were treated with ssRNA40-Alexa 546 followed by fixation, permeablization and staining for TLR8 using anti-TLR8 Alexa 488 conjugate or isotype control IgG1/Kappa Alexa 488. TLR8 detection is shown along the horizontal axis, and ssRNA40 Alexa 546 detection is shown along the vertical axis. Direct excitation (488 and 561 nm lasers) of both fluorophores (Alexa 488 and Alexa 546) allowed quantitation of cells staining positive for TLR8 (anti-TLR8-Alexa488) and cells that had taken up ssRNA40-Alexa 546. Single-stranded RNA40 binds TLR8 (flow cytometry FRET assay). A representative experiment is shown from three independent experiments with similar results. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0104039) licensed under a CC-BY license.

Western Blot: TLR8 Antibody (44C143) [NBP2-24917] - Analysis of TLR8 in human Ramos cell lysate. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Simple Western: TLR8 Antibody (44C143) [NBP2-24917] - Simple Western lane view shows a specific band for TLR8 in 0.5 mg/ml of Ramos (left) and Human Spleen (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody Image from the standard format of this antibody.

Reactivity:

Hu, Mu, Rb

Species Glossary

Reactivity: Hu, Mu, Rb

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-321A

Alt Catalog# IMG-321A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24917AF350
Alexa Fluor 405	NBP2-24917AF405
Alexa Fluor 488	NBP2-24917AF488
Alexa Fluor 532	NBP2-24917AF532
Alexa Fluor 594	NBP2-24917AF594
Alexa Fluor 647	NBP2-24917AF647
Alexa Fluor 700	NBP2-24917AF700
Alexa Fluor 750	NBP2-24917AF750
Allophycocyanin	NBP2-24917APC
Biotin	NBP2-24917B
CoraFluor 1	NBP2-24917CL1
DyLight 350	NBP2-24917UV
DyLight 405	NBP2-24917V
DyLight 488	NBP2-24917G
DyLight 550	NBP2-24917R
DyLight 594	NBP2-24917DL594
DyLight 650	NBP2-24917C
DyLight 680	NBP2-24917FR
DyLight 755	NBP2-24917IR
FITC	NBP2-24917F
HRP	NBP2-24917H
Janelia Fluor 525	NBP2-24917JF525
Janelia Fluor 549	NBP2-24917JF549
Janelia Fluor 646	NBP2-24917JF646
Janelia Fluor 669	NBP2-24917JF669
PE	NBP2-24817
PE	NBP2-24917PE
PerCP	NBP2-24917PCP
mFluor Violet 450 SE	NBP2-24917MFV450
mFluor Violet 500 SE	NBP2-24917MFV500
mFluor Violet 610 SE	NBP2-24917MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-24917SS
Azide and BSA Free	NBP2-77073

compare

(select up to 3 products from this page to compare)

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Inhibitor Family

Inhibitor Target

Isotype

Kit Type

Lysate Subcellular Fraction

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

GLI-1 Antibody - BSA Free

alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free

CD31/PECAM-1 Antibody (JC/70A) - BSA Free

YAP1 Antibody (2F12) - Isoform 5 - Azide and BSA Free

NQO-1 Antibody (A180) - BSA Free

CD4 Antibody - BSA Free

BST2 Antibody (120G8.04) - Azide and BSA Free

GAPDH Antibody

CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free

DRP1 Antibody - BSA Free

CD45 Antibody (30-F11) - BSA Free

TLR8 Antibody (44C143) - BSA Free