Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [Alexa Fluor® 647]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 647

Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [Alexa Fluor® 647] Summary

Immunogen
A synthetic peptide surrounding the phosphorylated serine 293 of the human Pyruvate Dehydrogenase E1-alpha subunit protein. [Swiss-Prot #P08559]
Modification
p Ser293
Localization
Mitochondrion matrix.
Specificity
This is specific for the phosphorylated Serine 293 form of the PDHE1 alpha protein. It is recommended that BSA rather than non-fat milk be used in membrane blocking and antibody dilution.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDHA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This Pyruvate Dehydrogenase E1-alpha subunit antibody is useful for Immunocytochemistry/immunofluorescence and Western blot, where a band is seen ~43 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [Alexa Fluor® 647]

  • EC 1.2.4.1
  • PDHA
  • PDHE1-A type I
  • PHE1APDHCE1A
  • pyruvate dehydrogenase (lipoamide) alpha 1
  • pyruvate dehydrogenase complex, E1-alpha polypeptide 1
  • pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 700 Labeled NB110-93479AF700

Additional Pyruvate Dehydrogenase E1-alpha subunit Products

Bioinformatics Tool for Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479AF647)

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Blogs on Pyruvate Dehydrogenase E1-alpha subunit.

Pyruvate Dehydrogenase E1-alpha subunit: Understanding the Catalysts in Glycolysis
Pyruvate Dehydrogenase E1-alpha subunit (PDHA1) is one of multiple enzymes in a mitochondrial complex, called the Pyruvate Dehydrogenase (PDH) complex, involved in the transition between glycolysis and the tricarboxylic acid (TCA) cycle during...  Read full blog post.

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Bioinformatics

Gene Symbol PDHA1