NQO-1 Antibody (A180)

Images

 
Western Blot: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO1 in A549 lysates using NB 200-209. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.
Immunocytochemistry/ Immunofluorescence: NQO-1 Antibody (A180) [NB200-209] - NQO1 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Analysis of NQO1 in human breast cancer using DAB with hematoxylin counterstain.
Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO-1 in Human A549 Cell Line by Flow Cytometry. Human A549 cell line was stained with Mouse Anti- NQO-1 Monoclonal Antibody (Catalog # NB200-209, filled ...read more
Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Immunohistochemistry showing Anti-NQ01 on formalin fixed paraffin embedded kidney tissue
Flow (Intracellular): NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U87MG cells with NQO1 (A180) antibody NBP2-24917 (blue) along with a matched isotype control NBP2-27287 (orange). Cells ...read more
Simple Western: NQO-1 Antibody (A180) [NB200-209] - Simple Western lane view shows a specific band for NQO-1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
A180
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NQO-1 Antibody (A180) Summary

Immunogen
Full length recombinant NQO1 from human lung [UniProt# P15559]
Localization
Cytoplasmic (primarily), Nuclear (faintly)
Specificity
Does not cross-react with NQO2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NQO1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Flow Cytometry 1-2 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:1000
  • Flow (Intracellular)
Application Notes
This NQO1 Antibody (A180) is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections and Flow Cytometry. In Western blot a band can be seen at approx. 31 kDa representing NQO1. In ICC/IF, cytoplasmic staining was observed in U2OS cells. In IHC-P, staining was observed in the cytoplasm of human breast cancer tisSue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NQO-1 Lysate (NBL1-13761)
Publications
Read Publications using
NB200-209 in the following applications:

  • 1 publication
  • 3 publications
  • IP
    2 publications
  • WB
    12 publications

Reactivity Notes

Human, rat, monkey and dog. Mouse reactivity reported in scientific literature (PMID: 24475200)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for NQO-1 Antibody (A180)

  • Azoreductase
  • DHQU
  • DIA4
  • DIA4azoreductase
  • diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
  • Diaphorase-4
  • Dioxin-inducible 1
  • DTD
  • DTDEC 1.6.5.2
  • DT-diaphorase
  • Menadione Reductase
  • NAD(P)H dehydrogenase [quinone] 1
  • NAD(P)H dehydrogenase, quinone 1
  • NAD(P)H:menadione oxidoreductase 1
  • NAD(P)H:Quinone acceptor oxidoreductase type 1
  • NAD(P)H:quinone oxidoreductase 1
  • NAD(P)H:quinone oxireductase
  • NMOR1
  • NMOR1diaphorase-4
  • NQO1
  • NQO-1
  • Phylloquinone reductase
  • QR1
  • QR1NMORI
  • Quinone Reductase 1

Background

NAD(P)H:quinone oxidoreductase (NQO) is a ubiquitous flavoprotein enzyme which catalyzes the two-electron reduction/detoxification of quinones/quinones derivatives and it has two members - NQO1 (alias DT-diaphorase) which uses NADPH as a source of electrons, and NQO2 (NRH:quinone oxidoreductase 2) which employs dihydronicotinamide riboside (NRH) as electron donor. Expressed as cytosolic enzyme with maximum levels in liver, NQO1 is highly inducible by oxidants, electrophiles, phenolic compounds etc and its expression is regulated by Keap1/Nrf2/ARE pathway in mammals. NQO1 gene promotor contains multiple AREs (antioxidant response elements) and a functional XRE (xenobiotic response element), and AhR binding to XRE is critical for NQO1 induction by xenobiotics. Moreover, Nrf2/ARE and AhR/XRE have been proposed to coordinate in NQO1 expression regulation. Besides biotransformation, NQO1 also implicates in the maintenance of endogenous lipid-soluble antioxidants alpha-tocopherol and ubiquinone in their reduced/active forms, direct scavenging of superoxide anion radicals, stabilization of the tumor suppressor proteins including p53 and as a gatekeeper of the 20S proteasome pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NQO-1 Antibody (NB200-209)(26)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(3)
IP
(2)
WB
(12)
All Applications
Filter By Species
Human
(12)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB200-209 Applications Species
Shrishrimal S, Kosmacek EA, Chatterjee A et al. The SOD Mimic, MnTE-2-PyP, Protects from Chronic Fibrosis and Inflammation in Irradiated Normal Pelvic Tissues Antioxidants (Basel). 2017 Nov 06 [PMID: 29113120] (Mouse) Mouse
Petrillo S, Piermarini E, Pastore A et al. Nrf2-Inducers Counteract Neurodegeneration in Frataxin-Silenced Motor Neurons: Disclosing New Therapeutic Targets for Friedreich's Ataxia. Int J Mol Sci. 2017 Oct 18 [PMID: 29057804] (WB) WB
Simoni E, Serafini MM, Caporaso R et al. Targeting the Nrf2/Amyloid-Beta Liaison in Alzheimer's Disease: A Rational Approach. ACS Chem Neurosci Apr 25 2017 [PMID: 28421738] (Human) Human
Kim J, Lim J, Kang BY et al. Capillarisin augments anti-oxidative and anti-inflammatory responses by activating Nrf2/HO-1 signaling. Neurochem. Int. 2017 Feb 01 [PMID: 28161468] (Human) Human
Zhang T, Hu Q, Shi L et al. Equol Attenuates Atherosclerosis in Apolipoprotein E-Deficient Mice by Inhibiting Endoplasmic Reticulum Stress via Activation of Nrf2 in Endothelial Cells. PLoS ONE. Dec 1 2016 [PMID: 27907038] (WB, Mouse) WB Mouse
Qin T, Yin S, Yang J et al. Sinomenine attenuates renal fibrosis through Nrf2-mediated inhibition of oxidative stress and TGFbeta signaling. Toxicol. Appl. Pharmacol. May 17 2016 [PMID: 27211841] (WB, Human, Mouse) WB Human, Mouse
Qin T, Du R, Huang F et al. Sinomenine activation of Nrf2 signaling prevents hyperactive inflammation and kidney injury in a mouse model of obstructive nephropathy. Free Radic. Biol. Med. 2016 Jan 18 [PMID: 26795599] (WB, Human) WB Human
Bousova I, Bartikova H, Matouskova P et al. Cranberry extract-enriched diets increase NAD(P)H:quinone oxidoreductase and catalase activities in obese but not in nonobese mice. Nutr Res. 2015 Aug 08 [PMID: 26319613]
Qin S, Du R, Yin S et al. Nrf2 is essential for the anti-inflammatory effect of carbon monoxide in LPS-induced inflammation Inflamm. Res. 2015 Jul 01 [PMID: 26049867] (WB, Mouse) WB Mouse
Suyama K, Watanabe M, Sakabe K et al. GRP78 suppresses lipid peroxidation and promotes cellular antioxidant levels in glial cells following hydrogen peroxide exposure. PLoS ONE 2014 Jan 29 [PMID: 24475200] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Show All 26 Publications.

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Product General Protocols

View specific protocols for NQO-1 Antibody (NB200-209): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NQO-1 Antibody (NB200-209). (Showing 1 - 1 of 1 FAQs).

  1. How was it determined that NB200-209 antibody does not cross react with NQo2?
    • When this antibody was raised our collaborators performed the testing necessary to confirm that there was no cross-reactivity with NQO2. We do not have the lab notes available regarding the test that was used to determine this. We did a protein blast between human NQO1 and NQO2 and found that the identity is only 48% between the 2 sequences. Generally, we predict that identity must be 86%+ unless there is an empirical determination of cross-reactivity. Since it is listed in our notes then it has been tested and confirmed.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB200-209AF405
Alexa Fluor 488 Labeled NB200-209AF488
Alexa Fluor 647 Labeled NB200-209AF647
Alexa Fluor 700 Labeled NB200-209AF700
Allophycocyanin Labeled NB200-209APC
Biotin Labeled NB200-209B
DyLight 350 Labeled NB200-209UV
DyLight 405 Labeled NB200-209V
DyLight 488 Labeled NB200-209G
DyLight 550 Labeled NB200-209R
DyLight 650 Labeled NB200-209C
DyLight 680 Labeled NB200-209FR
DyLight 755 Labeled NB200-209IR
FITC Labeled NB200-209F
HRP Labeled NB200-209H
PE Labeled NB200-209PE
PerCP Labeled NB200-209PCP

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Bioinformatics

Gene Symbol NQO1
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