NQO-1 Antibody (A180)

Images

 
Western Blot: NQO-1 Antibody (A180) [NB200-209] - Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of NQO1 protein at 6, 12 and 24 hrs following 15 min ...read more
Immunocytochemistry/ Immunofluorescence: NQO-1 Antibody (A180) [NB200-209] - NQO1 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Analysis of NQO1 in human breast cancer using DAB with hematoxylin counterstain.
Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Western Blot: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO1 in A549 lysates using NB 200-209. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.
Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Immunohistochemistry showing Anti-NQ01 on formalin fixed paraffin embedded human kidney tissue
Flow (Intracellular): NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U87MG cells with NQO1 (A180) antibody NBP2-24917 (blue) along with a matched isotype control NBP2-27287 (orange). Cells ...read more
Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO-1 in Human A549 Cell Line by Flow Cytometry. Human A549 cell line was stained with Mouse Anti- NQO-1 Monoclonal Antibody (Catalog # NB200-209, filled ...read more
Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Simple Western: NQO-1 Antibody (A180) [NB200-209] - Simple Western lane view shows a specific band for NQO-1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
A180
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

NQO-1 Antibody (A180) Summary

Immunogen
Full length recombinant NQO1 from human lung [UniProt# P15559]
Localization
Cytoplasmic (primarily), Nuclear (faintly)
Specificity
Does not cross-react with NQO2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NQO1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Flow Cytometry 1-2 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:1000
  • Flow (Intracellular)
Application Notes
This NQO1 Antibody (A180) is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections and Flow Cytometry. In Western blot a band can be seen at approx. 31 kDa representing NQO1. In ICC/IF, cytoplasmic staining was observed in U2OS cells. In IHC-P, staining was observed in the cytoplasm of human breast cancer tisSue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NQO-1 Overexpression Lysate
Publications
Read Publications using
NB200-209 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • 3 publications
  • IP
    2 publications
  • WB
    22 publications

Reactivity Notes

Human, rat, monkey and dog. Mouse reactivity reported in scientific literature (PMID: 24475200)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for NQO-1 Antibody (A180)

  • Azoreductase
  • DHQU
  • DIA4
  • DIA4azoreductase
  • diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
  • Diaphorase-4
  • Dioxin-inducible 1
  • DTD
  • DTDEC 1.6.5.2
  • DT-diaphorase
  • Menadione Reductase
  • NAD(P)H dehydrogenase [quinone] 1
  • NAD(P)H dehydrogenase, quinone 1
  • NAD(P)H:menadione oxidoreductase 1
  • NAD(P)H:Quinone acceptor oxidoreductase type 1
  • NAD(P)H:quinone oxidoreductase 1
  • NAD(P)H:quinone oxireductase
  • NMOR1
  • NMOR1diaphorase-4
  • NQO1
  • NQO-1
  • Phylloquinone reductase
  • QR1
  • QR1NMORI
  • Quinone Reductase 1

Background

NAD(P)H:quinone oxidoreductase (NQO) is a ubiquitous flavoprotein enzyme which catalyzes the two-electron reduction/detoxification of quinones/quinones derivatives and it has two members - NQO1 (alias DT-diaphorase) which uses NADPH as a source of electrons, and NQO2 (NRH:quinone oxidoreductase 2) which employs dihydronicotinamide riboside (NRH) as electron donor. Expressed as cytosolic enzyme with maximum levels in liver, NQO1 is highly inducible by oxidants, electrophiles, phenolic compounds etc and its expression is regulated by Keap1/Nrf2/ARE pathway in mammals. NQO1 gene promotor contains multiple AREs (antioxidant response elements) and a functional XRE (xenobiotic response element), and AhR binding to XRE is critical for NQO1 induction by xenobiotics. Moreover, Nrf2/ARE and AhR/XRE have been proposed to coordinate in NQO1 expression regulation. Besides biotransformation, NQO1 also implicates in the maintenance of endogenous lipid-soluble antioxidants alpha-tocopherol and ubiquinone in their reduced/active forms, direct scavenging of superoxide anion radicals, stabilization of the tumor suppressor proteins including p53 and as a gatekeeper of the 20S proteasome pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NQO-1 Antibody (NB200-209)(39)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(3)
IP
(2)
WB
(22)
All Applications
Filter By Species
Human
(16)
Mouse
(10)
Rat
(3)
All Species
Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using NB200-209 Applications Species
Siegel D, Bersie S, Harris P et al. A redox-mediated conformational change in NQO1 controls binding to microtubules and alpha-tubulin acetylation Redox biology Dec 18 2020 [PMID: 33360352] (Human) Human
Sekiguchi M, Seki M, Kawai T et al. Integrated multiomics analysis of hepatoblastoma unravels its heterogeneity and provides novel druggable targets NPJ Precis Oncol Jul 7 2020 [PMID: 32656360] (WB, Human) WB Human
Chatterjee A, Kosmacek EA, Shrishrimal S et al. MnTE-2-PyP, a manganese porphyrin, reduces cytotoxicity caused by irradiation in a diabetic environment through the induction of endogenous antioxidant defenses Redox Biol Apr 21 2020 [PMID: 32361681] (WB, Human) WB Human
Shrishrimal S, Chatterjee A, Kosmacek Ea et Al. Manganese porphyrin, MnTE-2-PyP, treatment protects the prostate from radiation-induced fibrosis (RIF) by activating the NRF2 signaling pathway and enhancing SOD2 and sirtuin activity Free Radic. Biol. Med. Mar 25 2020 [PMID: 32222469] (WB, Mouse) WB Mouse
Serafini M, Catanzaro M, Fagiani F et al. Modulation of Keap1/Nrf2/ARE Signaling Pathway by Curcuma- and Garlic-Derived Hybrids Front. Pharmacol. Jan 28 2020 [PMID: 32047434] (WB, Human) WB Human
La Rosa P, Russo M, D'Amico J et al. Nrf2 Induction Re-establishes a Proper Neuronal Differentiation Program in Friedreich's Ataxia Neural Stem Cells Front Cell Neurosci Jul 31 2019 [PMID: 31417369] (WB, Mouse) WB Mouse
Jeong J W, Ji S Y et al. Fermented Sea Tangle (Laminaria japonica Aresch) Suppresses RANKL-Induced Osteoclastogenesis by Scavenging ROS in RAW 264.7 Cells. Foods Jul 26 2019 [PMID: 31357503] (WB, Mouse) WB Mouse
Peng J, Zhou F, Wang Y et al. Differential response to lead toxicity in rat primary microglia and astrocytes. Life Sci. Nov 16 2018 [PMID: 30476502] (WB, Rat) WB Rat
Wang Y, Feng F, Liu M et al. Resveratrol ameliorates sepsis-induced acute kidney injury in a pediatric rat model via Nrf2 signaling pathway. Exp Ther Med. [PMID: 30214546] (WB, Rat) WB Rat
Bricambert J, Alves-Guerra MC, Esteves P et al. The histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity Nat Commun May 29 2018 [PMID: 29844386] (WB, Mouse) WB Mouse
Show All 39 Publications.

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Product General Protocols

View specific protocols for NQO-1 Antibody (NB200-209): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NQO-1 Antibody (NB200-209). (Showing 1 - 1 of 1 FAQs).

  1. How was it determined that NB200-209 antibody does not cross react with NQo2?
    • When this antibody was raised our collaborators performed the testing necessary to confirm that there was no cross-reactivity with NQO2. We do not have the lab notes available regarding the test that was used to determine this. We did a protein blast between human NQO1 and NQO2 and found that the identity is only 48% between the 2 sequences. Generally, we predict that identity must be 86%+ unless there is an empirical determination of cross-reactivity. Since it is listed in our notes then it has been tested and confirmed.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB200-209AF350
Alexa Fluor 405 NB200-209AF405
Alexa Fluor 488 NB200-209AF488
Alexa Fluor 532 NB200-209AF532
Alexa Fluor 594 NB200-209AF594
Alexa Fluor 647 NB200-209AF647
Alexa Fluor 700 NB200-209AF700
Alexa Fluor 750 NB200-209AF750
Allophycocyanin NB200-209APC
Biotin NB200-209B
DyLight 350 NB200-209UV
DyLight 405 NB200-209V
DyLight 488 NB200-209G
DyLight 550 NB200-209R
DyLight 594 NB200-209DL594
DyLight 650 NB200-209C
DyLight 680 NB200-209FR
DyLight 755 NB200-209IR
FITC NB200-209F
HRP NB200-209H
Janelia Fluor 549 NB200-209JF549
Janelia Fluor 646 NB200-209JF646
PE NB200-209PE
PerCP NB200-209PCP

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Bioinformatics

Gene Symbol NQO1