NQO-1 Antibody (A180)

Images

 
Biological Strategies: Western Blot: NQO-1 Antibody (A180) [NB200-209] - Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of NQO1 protein at 6, 12 and ...read more
Immunocytochemistry/ Immunofluorescence: NQO-1 Antibody (A180) [NB200-209] - NQO1 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Analysis of NQO1 in human breast cancer using DAB with hematoxylin counterstain.
Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Western Blot: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO1 in A549 lysates using NB 200-209. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.
Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Immunohistochemistry showing Anti-NQ01 on formalin fixed paraffin embedded human kidney tissue
Flow (Intracellular): NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U87MG cells with NQO1 (A180) antibody NBP2-24917 (blue) along with a matched isotype control NBP2-27287 (orange). Cells ...read more
Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO-1 in Human A549 Cell Line by Flow Cytometry. Human A549 cell line was stained with Mouse Anti- NQO-1 Monoclonal Antibody (Catalog # NB200-209, filled ...read more
Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Simple Western: NQO-1 Antibody (A180) [NB200-209] - Simple Western lane view shows a specific band for NQO-1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of HO-1, NQO1 and GCLm proteins at 6, 12 and 24 hrs following 15 min treatment of BEAS-2B cells with the oil ...read more
Equol attenuated ER stress by activating Nrf2 in vitro and in vivo.(A) HUVECs were incubated with different concentrations (1, 10 and 100 nM) of equol for 24 h before treatment with t-BHP (50 uM) for another 6 h. The ...read more
Spontaneously metastasizing tumor cells are more frequently positive for dormancy or stem-like markers compared to intravenously injected tumor cells.a Representative immunofluorescence images of NR2F1 expression in ...read more
GPR50 promotes ligand-independent activation of T beta RI signaling. a HEK293T cells expressing myc-Smad3, and GPR50 delta 4 or GPR50wt were starved overnight and stimulated with TGF beta (2 ng/mL; 1 h). Smad3 ...read more
APE1 is acetylated after binding to AP sites in the chromatin. (A) BJ-hTERT cells were treated with MX (50 mM) for the indicated times. IF was performed using anti-APE1 and anti-AcAPE1, and counterstaining with DAPI was ...read more
FKBP51/Hsp90 bind to GluR1-type AMPA receptors to regulate trafficking. A, Representative Western blottings form biotinylation assays of receptor endocytosis was performed on ex vivo slices, as described in Materials ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP
Clone
A180
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

NQO-1 Antibody (A180) Summary

Immunogen
Full length recombinant NQO1 from human lung [UniProt# P15559]
Localization
Cytoplasmic (primarily), Nuclear (faintly)
Specificity
Does not cross-react with NQO2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NQO1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry 1-2 ug/mL
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:1000
  • Simple Western 1:100
  • Western Blot 1:1000
Application Notes
In Western blot a band can be seen at approx. 31 kDa representing NQO1. In ICC/IF, cytoplasmic staining was observed in U2OS cells. In IHC-P, staining was observed in the cytoplasm of human breast cancer tisSue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NQO-1 Overexpression Lysate
Publications
Read Publications using
NB200-209 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24475200)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for NQO-1 Antibody (A180)

  • Azoreductase
  • DHQU
  • DIA4
  • DIA4azoreductase
  • diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
  • Diaphorase-4
  • Dioxin-inducible 1
  • DTD
  • DTDEC 1.6.5.2
  • DT-diaphorase
  • Menadione Reductase
  • NAD(P)H dehydrogenase [quinone] 1
  • NAD(P)H dehydrogenase, quinone 1
  • NAD(P)H:menadione oxidoreductase 1
  • NAD(P)H:Quinone acceptor oxidoreductase type 1
  • NAD(P)H:quinone oxidoreductase 1
  • NAD(P)H:quinone oxireductase
  • NMOR1
  • NMOR1diaphorase-4
  • NQO1
  • NQO-1
  • Phylloquinone reductase
  • QR1
  • QR1NMORI
  • Quinone Reductase 1

Background

NAD(P)H:quinone oxidoreductase (NQO) is a ubiquitous flavoprotein enzyme which catalyzes the two-electron reduction/detoxification of quinones/quinones derivatives and it has two members - NQO1 (alias DT-diaphorase) which uses NADPH as a source of electrons, and NQO2 (NRH:quinone oxidoreductase 2) which employs dihydronicotinamide riboside (NRH) as electron donor. Expressed as cytosolic enzyme with maximum levels in liver, NQO1 is highly inducible by oxidants, electrophiles, phenolic compounds etc and its expression is regulated by Keap1/Nrf2/ARE pathway in mammals. NQO1 gene promotor contains multiple AREs (antioxidant response elements) and a functional XRE (xenobiotic response element), and AhR binding to XRE is critical for NQO1 induction by xenobiotics. Moreover, Nrf2/ARE and AhR/XRE have been proposed to coordinate in NQO1 expression regulation. Besides biotransformation, NQO1 also implicates in the maintenance of endogenous lipid-soluble antioxidants alpha-tocopherol and ubiquinone in their reduced/active forms, direct scavenging of superoxide anion radicals, stabilization of the tumor suppressor proteins including p53 and as a gatekeeper of the 20S proteasome pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NQO-1 Antibody (NB200-209)(53)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IF/IHC
(2)
IHC-P
(3)
IP
(2)
WB
(32)
All Applications
Filter By Species
Human
(22)
Mouse
(16)
Rat
(3)
All Species
Showing Publications 1 - 10 of 53. Show All 53 Publications.
Publications using NB200-209 Applications Species
Meister ML, Feresin RG Blackberry consumption protects against e-cigarette-induced vascular oxidative stress in mice Food & function 2023-11-08 [PMID: 37937402]
Zarcone G, Lenski M, Martinez T et al. Impact of Electronic Cigarettes, Heated Tobacco Products and Conventional Cigarettes on the Generation of Oxidative Stress and Genetic and Epigenetic Lesions in Human Bronchial Epithelial BEAS-2B Cells Toxics 2023-10-10 [PMID: 37888697] (WB, Human)

Details:
1:1000 WB dilution
WB Human
Meister ML, Najjar RS, Danh JP et al. Berry consumption mitigates the hypertensive effects of a high-fat, high-sucrose diet via attenuation of renal and aortic AT1R expression resulting in improved endothelium-derived NO bioavailability The Journal of nutritional biochemistry 2022-11-23 [PMID: 36435288] (WB, Human, Mouse) WB Human, Mouse
Petrillo S, D’Amico J, Nicita F et al. Antioxidant Response in Human X-Linked Adrenoleukodystrophy Fibroblasts Antioxidants 2022-10-28 [PMID: 36358497] (WB, Human) WB Human
Moore TM, Cheng L, Wolf DM et al. Parkin regulates adiposity by coordinating mitophagy with mitochondrial biogenesis in white adipocytes Nature communications 2022-11-04 [PMID: 36333379] (WB, Mouse) WB Mouse
Li Q, Fadoul G, Ikonomovic M et al. Sulforaphane promotes white matter plasticity and improves long-term neurological outcomes after ischemic stroke via the Nrf2 pathway Free radical biology & medicine 2022-10-13 [PMID: 36244590] (IF/IHC, Mouse) IF/IHC Mouse
Watzky M, Huard S, Juricek L et al. Hexokinase 2 is a transcriptional target and a positive modulator of AHR signalling Nucleic acids research 2022-05-24 [PMID: 35609998] (WB, Human) WB Human
Wu D, Sun Q, Wei W et al. Nrf2-mediated protective effect of alpha-lipoic acid on synaptic oxidative damage and inhibition of PKC/ERK/CREB pathway in bisphenol A-exposed HT-22 cells Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023-10-17 [PMID: 37858839] (WB, Mouse) WB Mouse
Wamsley NT, Wilkerson EM, Guan L et al. Targeted proteomic quantitation of NRF2 signaling and predictive biomarkers in HNSCC Molecular & cellular proteomics : MCP 2023-09-14 [PMID: 37716475] (WB, Human) WB Human
Show All 53 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NQO-1 Antibody (NB200-209). (Showing 1 - 1 of 1 FAQs).

  1. How was it determined that NB200-209 antibody does not cross react with NQo2?
    • When this antibody was raised our collaborators performed the testing necessary to confirm that there was no cross-reactivity with NQO2. We do not have the lab notes available regarding the test that was used to determine this. We did a protein blast between human NQO1 and NQO2 and found that the identity is only 48% between the 2 sequences. Generally, we predict that identity must be 86%+ unless there is an empirical determination of cross-reactivity. Since it is listed in our notes then it has been tested and confirmed.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NQO1