NQO-1 Antibody (A180)


Western Blot: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO1 in A549 lysates using NB 200-209. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.
Immunocytochemistry/ Immunofluorescence: NQO-1 Antibody (A180) [NB200-209] - NQO1 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Analysis of NQO1 in human breast cancer using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Immunohistochemistry showing Anti-NQ01 on formalin fixed paraffin embedded kidney tissue
Simple Western: NQO-1 Antibody (A180) [NB200-209] - Simple Western lane view shows a specific band for NQO-1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
1.0 mg/ml

NQO-1 Antibody (A180) Summary

Full length recombinant NQO1 from human lung [UniProt# P15559]
Cytoplasmic (primarily), Nuclear (faintly)
Does not cross-react with NQO2.
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
1.0 mg/ml
Protein G purified


Application Notes
This NQO1 Antibody (A180) is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections and Flow Cytometry. In Western blot a band can be seen at ~ 31 kDa representing NQO1. In ICC/IF, cytoplasmic staining was observed in U2OS cells. In IHC-P, staining was observed in the cytoplasm of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
31 kDa
Read Publications using
NB200-209 in the following applications:

  • 1 publication
  • 3 publications
  • IP
    2 publications
  • WB
    10 publications

Reactivity Notes

Human, rat, monkey and dog. Mouse reactivity reported in scientific literature (PMID: 24475200)

Alternate Names for NQO-1 Antibody (A180)

  • Azoreductase
  • DHQU
  • DIA4
  • DIA4azoreductase
  • diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
  • Diaphorase-4
  • Dioxin-inducible 1
  • DTD
  • DT-diaphorase
  • Menadione Reductase
  • NAD(P)H dehydrogenase [quinone] 1
  • NAD(P)H dehydrogenase, quinone 1
  • NAD(P)H:menadione oxidoreductase 1
  • NAD(P)H:Quinone acceptor oxidoreductase type 1
  • NAD(P)H:quinone oxidoreductase 1
  • NAD(P)H:quinone oxireductase
  • NMOR1
  • NMOR1diaphorase-4
  • NQO1
  • Phylloquinone reductase
  • QR1
  • Quinone Reductase 1


NAD(P)H:quinone oxidoreductase (NQO) is a ubiquitous flavoprotein enzyme which catalyzes the two-electron reduction/detoxification of quinones/quinones derivatives and it has two members - NQO1 (alias DT-diaphorase) which uses NADPH as a source of electrons, and NQO2 (NRH:quinone oxidoreductase 2) which employs dihydronicotinamide riboside (NRH) as electron donor. Expressed as cytosolic enzyme with maximum levels in liver, NQO1 is highly inducible by oxidants, electrophiles, phenolic compounds etc and its expression is regulated by Keap1/Nrf2/ARE pathway in mammals. NQO1 gene promotor contains multiple AREs (antioxidant response elements) and a functional XRE (xenobiotic response element), and AhR binding to XRE is critical for NQO1 induction by xenobiotics. Moreover, Nrf2/ARE and AhR/XRE have been proposed to coordinate in NQO1 expression regulation. Besides biotransformation, NQO1 also implicates in the maintenance of endogenous lipid-soluble antioxidants alpha-tocopherol and ubiquinone in their reduced/active forms, direct scavenging of superoxide anion radicals, stabilization of the tumor suppressor proteins including p53 and as a gatekeeper of the 20S proteasome pathway.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NQO-1 Antibody (NB200-209)(21)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB200-209 Applications Species
Qin T, Yin S, Yang J et al. Sinomenine attenuates renal fibrosis through Nrf2-mediated inhibition of oxidative stress and TGFbeta signaling. Toxicol. Appl. Pharmacol. May 17 2016 12:00AM [PMID:27211841] (WB, Human, Mouse) WB Human, Mouse
Qin T, Du R, Huang F et al. Sinomenine activation of Nrf2 signaling prevents hyperactive inflammation and kidney injury in a mouse model of obstructive nephropathy. Free Radic. Biol. Med. 2016 Jan 18 [PMID:26795599] (WB, Human) WB Human
Bousova I, Bartikova H, Matouskova P et al. Cranberry extract-enriched diets increase NAD(P)H:quinone oxidoreductase and catalase activities in obese but not in nonobese mice. Nutr Res. 2015 Aug 08 [PMID:26319613]
Qin S, Du R, Yin S et al. Nrf2 is essential for the anti-inflammatory effect of carbon monoxide in LPS-induced inflammation Inflamm. Res. 2015 Jul 01 [PMID:26049867] (WB, Mouse) WB Mouse
Suyama K, Watanabe M, Sakabe K et al. GRP78 suppresses lipid peroxidation and promotes cellular antioxidant levels in glial cells following hydrogen peroxide exposure. PLoS ONE 2014 Jan 29 [PMID:24475200] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Zhang T, Liang X, Shi L et al. Estrogen Receptor and PI3K/Akt Signaling Pathway Involvement in S-(-)Equol-Induced Activation of Nrf2/ARE in Endothelial Cells. PLoS One. 2013 Nov 19 [PMID:24260155] (WB, Human) WB Human
Wang B, Zhu X, Kim Y et al. Histone deacetylase inhibition activates transcription factor Nrf2 and protects against cerebral ischemic damage. Free Radic Biol Med. 2012 [PMID:22226832]
Gao M, Singh A, Macri K et al. Antioxidant components of naturally-occurring oils exhibit marked anti-inflammatory activity in epithelial cells of the human upper respiratory system. Respir Res;12(1):92. 2011 Jul 13. [PMID:21752292]
Solis LM, Behrens C, Dong W, Suraokar M, Ozburn NC, Moran CA, Corvalan AH, Biswal S, Swisher SG, Bekele BN, Minna JD, Stewart DJ, Wistuba II. Nrf2 and Keap1 abnormalities in non-small cell lung carcinoma and association with clinicopathologic features. Clin Cancer Res;16(14):3743-53. 2010 Jul 15. [PMID:20534738] (IHC-P, Human) IHC-P Human
Malhotra D, Thimmulappa R, Vij N et al. Heightened endoplasmic reticulum stress in the lungs of patients with chronic obstructive pulmonary disease: the role of Nrf2-regulated proteasomal activity. Am J Respir Crit Care Med;180(12):1196-207. 2009 Dec 15. [PMID:19797762]
Show All 21 Publications.

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Product General Protocols

View specific protocols for NQO-1 Antibody (NB200-209): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NQO-1 Antibody (NB200-209). (Showing 1 - 1 of 1 FAQs).

  1. How was it determined that NB200-209 antibody does not cross react with NQo2?
    • When this antibody was raised our collaborators performed the testing necessary to confirm that there was no cross-reactivity with NQO2. We do not have the lab notes available regarding the test that was used to determine this. We did a protein blast between human NQO1 and NQO2 and found that the identity is only 48% between the 2 sequences. Generally, we predict that identity must be 86%+ unless there is an empirical determination of cross-reactivity. Since it is listed in our notes then it has been tested and confirmed.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB200-209AF405
Alexa Fluor (R) 488 Labeled NB200-209AF488
Alexa Fluor (R) 647 Labeled NB200-209AF647
Alexa Fluor (R) 700 Labeled NB200-209AF700
Allophycocyanin Labeled NB200-209APC
Biotin Labeled NB200-209B
DyLight 350 Labeled NB200-209UV
DyLight 405 Labeled NB200-209V
DyLight 405LS Labeled NB200-209V3
DyLight 488 Labeled NB200-209G
DyLight 550 Labeled NB200-209R
DyLight 650 Labeled NB200-209C
DyLight 680 Labeled NB200-209FR
DyLight 755 Labeled NB200-209IR
FITC Labeled NB200-209F
HRP Labeled NB200-209H
PE Labeled NB200-209PE
PerCP Labeled NB200-209PCP

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Contact Information

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Gene Symbol NQO1

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