Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [DyLight 488]

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IP, WB, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 488

Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [DyLight 488] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: A synthetic peptide surrounding the phosphorylated serine 293 of the human Pyruvate Dehydrogenase E1-alpha subunit protein. [Swiss-Prot: #P08559]
• Modification: p Ser293
• Localization: Mitochondrion matrix
• Specificity: This is specific for the phosphorylated Serine 293 form of the PDHE1 alpha protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDHA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB110-93479. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [DyLight 488]

• EC 1.2.4.1
• PDHA
• PDHE1-A type I
• PHE1APDHCE1A
• pyruvate dehydrogenase (lipoamide) alpha 1
• pyruvate dehydrogenase complex, E1-alpha polypeptide 1
• pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Background

The pyruvate dehydrogenase complex (PDC) is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1 or PDHE1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex. Enhanced expression of pyruvate dehydrogenase kinase-1 (PDK-1) results in phosphorylation of PDHE1 alpha at Serine 293 which in turn produces nearly complete inhibition of the pyruvate dehydrogenase complex (PDC). A recent study shows that inhibition of PDC activity in cancer cells restores normoxic stablization of HIF-1 alpha by glycolytic metabolites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDHA1