YAP1 Antibody (2F12)

Images

 
Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - YAP1 monoclonal antibody (M01), clone 2F12. Analysis of YAP1 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of monoclonal antibody to YAP1 on HeLa cell. Antibody concentration 30 ug/ml.
Immunohistochemistry-Paraffin: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of monoclonal antibody to YAP1 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 3 ug/ml.
Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of YAP1 expression in transfected 293T cell line by YAP1 monoclonal antibody (M01), clone 2F12.Lane 1: YAP1 transfected lysate (Predicted MW: 54.5 KDa).Lane ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
2F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

YAP1 Antibody (2F12) Summary

Immunogen
YAP1 (NP_006097, 53 a.a. ~ 161 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.QIVHVRGDSETDLEALFNAVMNPKTANVPQTVPMRLRKLPDSFFKPPEPKSHSRQASTDAGTAGALTPQHVRAHSSPASLQLGAVSPGTLTPTGVVSGPAATPTAQHLR
Localization
Cytoplasmic and Nuclear
Specificity
YAP1 - Yes-associated protein 1, 65kDa
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
YAP1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 30 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 3 ug/ml
Publications
Read Publications using
H00010413-M01 in the following applications:

  • 5 publications
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in the scientific literature (PMID: 23791527). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for YAP1 Antibody (2F12)

  • 65 kDa Yes-associated protein
  • YAP
  • YAP1
  • YAP2
  • YAP65
  • YAP65yes-associated protein 2
  • Yes-associated protein 1
  • Yes-associated protein 1, 65kDa
  • yes-associated protein beta
  • yes-associated protein delta
  • YKI
  • Yorkie Homolog

Background

This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-90187
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB110-58359
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
NBP1-81763
Species: Hu
Applications: IHC, IHC-P
NBP1-86860
Species: Hu
Applications: WB, IHC, IHC-P
H00006901-M12
Species: Hu, Mu, Rt
Applications: WB, ELISA
H00060485-M02
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP1-48017
Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P
NBP1-87757
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00006789-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, PLA
NBP2-24737
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-84161
Species: Hu
Applications: IHC, IHC-P
NBP2-43581
Species: Hu
Applications: WB, ICC/IF, IP
NBP2-14085
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB200-199
Species: Hu, Mu
Applications: WB, IP, PLA
AF3254
Species: Hu, Mu, Rt
Applications: WB
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
H00010413-M01
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for YAP1 Antibody (H00010413-M01)(27)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(5)
IP
(1)
WB
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using H00010413-M01 Applications Species
Jang W, Kim T, Koo JS et al. Mechanical cue-induced YAP instructs Skp2-dependent cell cycle exit and oncogenic signaling EMBO J. 2017 Jul 03 [PMID: 28673931] (ICC/IF, Human) ICC/IF Human
Korotkevich E, Niwayama R, Courtois A et al. The Apical Domain Is Required and Sufficient for the First Lineage Segregation in the Mouse Embryo. Dev Cell 2017 Feb 6 [PMID: 28171747]
Suzuki M, Kondo A, Ogino I et al. Overexpression of TEAD4 in atypical teratoid/rhabdoid tumor: New insight to the pathophysiology of an aggressive brain tumor. Pediatr Blood Cancer 2016 Dec 14 [PMID: 27966820]
Junemann J, Birgin G, Erdmann J et al. Toxin A of the nosocomial pathogen Clostridium difficile induces primary effects in the proteome of HEp?2 cells. Proteomics Clin Appl 2016 Nov 10 [PMID: 27860399]
Maitre JL, Turlier H, Illukkumbura R et al. Asymmetric division of contractile domains couples cell positioning and fate specification. Nature 2016 Aug 18 [PMID: 27487217]
Abbassi L, Malki S, Cockburn K et al. Multiple Mechanisms Cooperate to Constitutively Exclude the Transcriptional Co-Activator YAP from the Nucleus During Murine Oogenesis. Biol Reprod 2016 May [PMID: 26985001]
Alarcon VB, Marikawa Y. Statins inhibit blastocyst formation by preventing geranylgeranylation. Mol. Hum. Reprod. May 1 2016 [PMID: 26908642] (ICC/IF, Mouse) ICC/IF Mouse
Yoyooka Y, Oka S, Fujimori T. Early preimplantation cells expressing Cdx2 exhibit plasticity of specification to TE and ICM lineages through positional changes. Dev Biol 2016 Jan 21 [PMID: 26806703]
Kim M, Kim M, Park SJ et al. Role of Angiomotin-like 2 mono-ubiquitination on YAP inhibition. EMBO Rep. 2015 Nov 23 [PMID: 26598551] (ICC/IF, Human) ICC/IF Human
Hirate Y, Hirahara S, Inoue K et al. Par-aPKC-dependent and -independent mechanisms cooperatively control cell polarity, Hippo signaling, and cell positioning in 16-cell stage mouse embryos. Dev Growth Differ. 2015 Oct 09 [PMID: 26450797]
Show All 27 Publications.

Reviews for YAP1 Antibody (H00010413-M01) (0)

There are no reviews for YAP1 Antibody (H00010413-M01). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for YAP1 Antibody (H00010413-M01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional YAP1 Products

Bioinformatics Tool for YAP1 Antibody (H00010413-M01)

Discover related pathways, diseases and genes to YAP1 Antibody (H00010413-M01). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for YAP1 Antibody (H00010413-M01)

Discover more about diseases related to YAP1 Antibody (H00010413-M01).
 

Pathways for YAP1 Antibody (H00010413-M01)

View related products by pathway.

PTMs for YAP1 Antibody (H00010413-M01)

Learn more about PTMs related to YAP1 Antibody (H00010413-M01).
 

Research Areas for YAP1 Antibody (H00010413-M01)

Find related products by research area.

Blogs on YAP1.

YAP1 - a transcription co-activator and the downstream target of Hippo pathway
YAP1 (Yes-associated protein 1) is a  transcriptional co-activator which acts as a major effector of Hippo signaling pathway that regulates organ size/ tissue homeostasis and cell proliferation, and is an established oncogene (1).  Hippo sig...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our YAP1 Antibody (2F12) and receive a gift card or discount.

Bioinformatics

Gene Symbol YAP1
Entrez
OMIM
Uniprot