Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody

Images

 
Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Rate of aerobic glycolysis in the presence of BA.MEF were treated with 10 uM BA or DMSO (0.1%) for 16 h before they were ...read more
Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] antibody (1:250) was tested in HeLa cells with ...read more
Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Detection of PDHE1 alpha [S293] in an in vitro autophosphorylation of PDH complex in response to different stimulants: (-) none, ...read more
Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - PDH activity and protein levels of PDK4 and p-PDH in hindlimb skeletal muscle. Samples were resolved by electrophoresis, ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IB, ICC/IF, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody Summary

Immunogen
A synthetic peptide surrounding the phosphorylated serine 293 of the human Pyruvate Dehydrogenase E1-alpha subunit protein. [Swiss-Prot: #P08559]
Modification
p Ser293
Localization
Mitochondrion matrix
Specificity
This is specific for the phosphorylated Serine 293 form of the PDHE1 alpha protein. It is recommended that BSA rather than non-fat milk be used in membrane blocking and antibody dilution.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDHA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunoprecipitation
  • Knockout Validated
Application Notes
In Western blot, a band is seen ~43 kDa. Use in Immunoprecipitation reported in scientific literature (PMID: 27450723). Knockout validation (PMID: 31742248). Use in FLOW reported in scientific literature (PMID:31900478).
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Pyruvate Dehydrogenase E1-alpha subunit Overexpression Lysate
Reviewed Applications
Read 5 Reviews rated 4.8
using
NB110-93479 in the following applications:

Publications
Read Publications using
NB110-93479 in the following applications:

  • 1 publication
  • IB
    1 publication
  • IP
    2 publications
  • KO
    1 publication
  • WB
    28 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody

  • EC 1.2.4.1
  • PDHA
  • PDHE1-A type I
  • PHE1APDHCE1A
  • pyruvate dehydrogenase (lipoamide) alpha 1
  • pyruvate dehydrogenase complex, E1-alpha polypeptide 1
  • pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Background

Pyruvate dehydrogenase (PDH) complex is localized to mitochondrial matrix, wherein it catalyzes the irreversible oxidative decarboxylation of pyruvate to generate acetyl-CoA, NADH, and CO2. The PDH complex contains three primary enzyme components: pyruvate dehydrogenase (E1 or PDHA- heterotetramer of two alpha- and two beta-subunits), dihydrolipoamide transacetylase (E2 or DLAT), and dihydrolipoamide dehydrogenase (E3 or DLD). The activity of PDH is controlled by specific E1 kinase and phospho-E1-phosphatase enzymes, which respectively inactivate and activate PDH complex by phosphorylation and dephosphorylation of E1alpha-subunit's serine residues. PDK family kinases mediated phosphorylation at Ser-293 blocks the access to active site leading to inactivation of the enzyme and vice versa. E3-binding protein (E3BP) is another component which is required for proper interaction of E2 and E3 components, and the overall complex contains 12 copies of E3, 30 copies of E1, 60 copies of E2, and 12 copies of E3BP. Acetyl-CoA generated gets utilized in TCA (citric acid/Krebs cycle) where it reacts with oxaloacetate to form citrate or it may also be used for biosynthesis of fatty acid or cholesterol. Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD), X-linked Leigh syndrome (X-LS) and it has also been implicated in ageing, glucose intolerance, cancer as well as Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479)(40)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Flow, IB, IP, KO, WB.


Filter By Application
Flow
(1)
IB
(1)
IP
(2)
KO
(1)
WB
(28)
All Applications
Filter By Species
Human
(13)
Mouse
(14)
Rat
(5)
All Species
Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using NB110-93479 Applications Species
Na YR, Jung D, Song J et al. Pyruvate dehydrogenase kinase is a negative regulator of interleukin-10 production in macrophages J Mol Cell Biol Jan 3 2020 [PMID: 31900478] (Flow, Mouse) Flow Mouse
Jebessa ZH, Shanmukha Kumar D, Dewenter M et al. The lipid droplet-associated protein ABHD5 protects the heart through proteolysis of HDAC4 Nat Metab Nov 1 2019 [PMID: 31742248] (IB, KO, Human, Mouse, Rat) IB, KO Human, Mouse, Rat
Lu H, Lu Y, Xie Y, et al. Rational combination with PDK1 inhibition overcomes cetuximab resistance in head and neck squamous cell carcinoma. JCI Insight. 2019 Oct 3 [PMID: 31578313] (WB, Human) WB Human
Jiang H, Jin J, Duan Y et al. Mitochondrial Uncoupling Coordinated With PDH Activation Safely Ameliorates Hyperglycemia Via Promoting Glucose Oxidation Diabetes Aug 30 2019 [PMID: 31471292]
Li J, Zhu X, Wang H et al. Akt1-mediated CPR cooling protection targets regulators of metabolism, inflammation and contractile function in mouse cardiac arrest PLoS ONE Aug 9 2019 [PMID: 31398213] (WB, Mouse) WB Mouse
Thapa D, Zhang M, Manning JR Loss of GCN5L1 in cardiac cells limits mitochondrial respiratory capacity under hyperglycemic conditions Physiol Rep Apr 1 2019 [PMID: 31033247] (WB, Rat) WB Rat
Zhong W, Xu M, Li C et al. ORP4L Extracts and Presents PIP2 from Plasma Membrane for PLCb3 Catalysis: Targeting It Eradicates Leukemia Stem Cells Cell Rep Feb 19 2019 [PMID: 30784597] (WB, Human) WB Human
Zhuang Y, Ly RC, Frazier CV et al. The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer. Cancer Metab Jan 24 2019 [PMID: 30697423] (WB, Human) WB Human
Kwong JQ, Huo J, Bround MJ et al. The mitochondrial calcium uniporter underlies metabolic fuel preference in skeletal muscle JCI Insight Nov 15 2018 [PMID: 30429366] (WB, Mouse) WB Mouse
Deng YH, Deng ZH, Hao H et al. MicroRNA-23a promotes colorectal cancer cell survival by targeting PDk4. Exp. Cell Res. Oct 18 2018 [PMID: 30342991] (WB, Human) WB Human
Show All 40 Publications.

Reviews for Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479) (5) 4.85

Average Rating: 4.8
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB110-93479:
Filter by Applications
WB
(5)
All Applications
Filter by Species
Human
(3)
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Pyruvate Dehydrogenase E1-alpha subunit NB110-93479
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reviewed by:
Anonymous
WB Human 07/10/2018
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Summary

ApplicationWestern Blot
Sample Testedplacenta cells
SpeciesHuman
LotA1
Western Blot Pyruvate Dehydrogenase E1-alpha subunit NB110-93479
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reviewed by:
Shahreen Amin
WB Human 06/12/2018
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Summary

ApplicationWestern Blot
Sample TestedNTera-2 human testicular embryonic carcinoma cell line,Differentiated Neuronal NTERA2 cells
SpeciesHuman
Lotp-1

Comments

CommentsThe site was similar in PDHA2 and we IP PDHA2 and its mutants at the site and probed with the antibody
Western Blot Pyruvate Dehydrogenase E1-alpha subunit NB110-93479
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reviewed by:
Anonymous
WB Mouse 05/12/2016
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Summary

ApplicationWestern Blot
Sample Testedprimary mouse macrophage lysates
SpeciesMouse

Blocking

Blocking Details5% Skim milk, 1h, room temperature

Primary Anitbody

Dilution Ratio1:1000, overnight, 4 deg, on a shaker

Secondary Antibody

Secondary DescriptionGoat anti-rabbit, peroxidase labeled
Secondary Concentration1:1000

Details

Detection NotesPierce Supersignal West Femto chemiluminiscent substrate, FluorChem HD instrument, 1-2 min exposure
Western Blot Pyruvate Dehydrogenase E1-alpha subunit NB110-93479
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reviewed by:
Silvina Meroni
WB Rat 01/14/2014
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Summary

ApplicationWestern Blot
Sample Testedrat Sertoli cells
SpeciesRat
LotK-1

Blocking

Blocking Details1X TBS, 0.1% Tween-20 with 5% m/w nonfat dry milk, 3 hours at room temperature

Primary Anitbody

Dilution Ratio1:1000 in 1X TBS, 0.1% Tween-20, over night at 4ºC

Secondary Antibody

Secondary DescriptionAnti rabbit secondary antibody Conjugated to Horseradish Peroxidase
Secondary Manufacturer Cat#Cell Signalling Technology #7074
Secondary Concentration1:4000

Details

Detection NotesPhototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody CST#7071
Western Blot Pyruvate Dehydrogenase E1-alpha subunit NB110-93479
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reviewed by:
Alexandra Grassian
WB Human 08/12/2010
View

Summary

ApplicationWestern Blot
Sample TestedMCF10A whole cell lysate, Sample Amount: 30ug
SpeciesHuman
CommentsVery clean, strong signal. I highly recommend this antibody.

Blocking

Blocking DetailsBlocking Buffer: 2% BSA, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 2% BSA, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Alexa 680 goat anti-rabbit, Secondary Ab Dilution Ratio: 1:5000
Secondary Manufacturer Cat#A21109

Details

Detection NotesDetection Method: Odyssey imager, Exposure Time: level 5, Positive Control: none, Negative Control: None, Specific Bands: 40 kDa

Comments

CommentsVery clean, strong signal. I highly recommend this antibody.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB110-93479AF405
Alexa Fluor 488 NB110-93479AF488
Alexa Fluor 647 NB110-93479AF647
Alexa Fluor 700 NB110-93479AF700
Biotin NB110-93479B
DyLight 405 NB110-93479V
DyLight 488 NB110-93479G
DyLight 550 NB110-93479R
DyLight 650 NB110-93479C
FITC NB110-93479F
HRP NB110-93479H

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Bioinformatics Tool for Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479)

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Diseases for Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479)

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Blogs on Pyruvate Dehydrogenase E1-alpha subunit.

Pyruvate Dehydrogenase E1-alpha subunit: Understanding the Catalysts in Glycolysis
Pyruvate Dehydrogenase E1-alpha subunit (PDHA1) is one of multiple enzymes in a mitochondrial complex, called the Pyruvate Dehydrogenase (PDH) complex, involved in the transition between glycolysis and the tricarboxylic acid (TCA) cycle during...  Read full blog post.

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Recent Reviews

4.8
5
4
4
1
3
0
2
0
1
0

Anonymous
07/10/2018
Application: WB
Species: Human

Shahreen Amin
06/12/2018
Application: WB
Species: Human

Anonymous
05/12/2016
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PDHA1