Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody

Images

 
Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Detection of PDHE1 alpha [S293] in an in vitro autophosphorylation of PDH complex in response to different stimulants: (-) none, ...read more
Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] antibody (1:250) was tested in HeLa cells with ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody Summary

Immunogen
A synthetic peptide surrounding the phosphorylated serine 293 of the human Pyruvate Dehydrogenase E1-alpha subunit protein. [Swiss-Prot #P08559]
Modification
p Ser293
Localization
Mitochondrion matrix.
Specificity
This is specific for the phosphorylated Serine 293 form of the PDHE1 alpha protein. It is recommended that BSA rather than non-fat milk be used in membrane blocking and antibody dilution.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDHA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunoprecipitation
Application Notes
This Pyruvate Dehydrogenase E1-alpha subunit antibody is useful for Immunocytochemistry/immunofluorescence and Western blot, where a band is seen ~43 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunoprecipitation reported in scientific literature (PMID: 27450723)
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Pyruvate Dehydrogenase E1-alpha subunit Lysate (NBL1-14241)
Reviewed Applications
Read 4 Reviews rated 5
using
NB110-93479 in the following applications:

Publications
Read Publications using
NB110-93479 in the following applications:

  • IP
    1 publication
  • WB
    16 publications

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody

  • EC 1.2.4.1
  • PDHA
  • PDHE1-A type I
  • PHE1APDHCE1A
  • pyruvate dehydrogenase (lipoamide) alpha 1
  • pyruvate dehydrogenase complex, E1-alpha polypeptide 1
  • pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Background

Pyruvate dehydrogenase (PDH) complex is localized to mitochondrial matrix, wherein it catalyzes the irreversible oxidative decarboxylation of pyruvate to generate acetyl-CoA, NADH, and CO2. The PDH complex contains three primary enzyme components: pyruvate dehydrogenase (E1 or PDHA- heterotetramer of two alpha- and two beta-subunits), dihydrolipoamide transacetylase (E2 or DLAT), and dihydrolipoamide dehydrogenase (E3 or DLD). The activity of PDH is controlled by specific E1 kinase and phospho-E1-phosphatase enzymes, which respectively inactivate and activate PDH complex by phosphorylation and dephosphorylation of E1alpha-subunit's serine residues. PDK family kinases mediated phosphorylation at Ser-293 blocks the access to active site leading to inactivation of the enzyme and vice versa. E3-binding protein (E3BP) is another component which is required for proper interaction of E2 and E3 components, and the overall complex contains 12 copies of E3, 30 copies of E1, 60 copies of E2, and 12 copies of E3BP. Acetyl-CoA generated gets utilized in TCA (citric acid/Krebs cycle) where it reacts with oxaloacetate to form citrate or it may also be used for biosynthesis of fatty acid or cholesterol. Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD), X-linked Leigh syndrome (X-LS) and it has also been implicated in ageing, glucose intolerance, cancer as well as Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-93479
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IP

Publications for Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479)(25)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(1)
WB
(16)
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Filter By Species
Human
(7)
Mouse
(6)
Rat
(3)
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Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB110-93479 Applications Species
Liu X, Zuo R, Bao Y et al. Down-regulation of PDK4 is Critical for the Switch of Carbohydrate Catabolism during Syncytialization of Human Placental Trophoblasts Sci Rep 2017 Aug 16 [PMID: 28814762] (WB, Human) WB Human
Gorga A, Rindone GM, Regueira M et al. PPARgamma activation regulates lipid droplet formation and lactate production in rat Sertoli cells. Cell Tissue Res. Apr 21 2017 [PMID: 28432465]
Bhosale G, Sharpe J, Koh A et al. Pathological consequences of MICU1 mutations on mitochondrial calcium signalling and bioenergetics Biochim. Biophys. Acta Jan 26 2017 [PMID: 28132899] (WB, Human) WB Human
Jun JC, Devera R, Unnikrishnan D et al. Adipose HIF-1alpha causes obesity by suppressing brown adipose tissue thermogenesis. J Mol Med (Berl) 2017 Mar [PMID: 27738746]
Zhong W, Yi Q, Xu B et al. ORP4L is essential for T-cell acute lymphoblastic leukemia cell survival. Nat Commun 2016 Sep 01 [PMID: 27581363] (WB) WB
Lu H, Li X, Lu Y et al. ASCT2 (SLC1A5) is an EGFR-associated protein that can be co-targeted by cetuximab to sensitize cancer cells to ROS-induced apoptosis Cancer Lett. 2016 Jul 19 [PMID: 27450723] (WB, IP) WB, IP
Rossi SP, Windschuettl S, Matzkin ME et al. Reactive oxygen species (ROS) production triggered by prostaglandin D2 (PGD2) regulates lactate dehydrogenase (LDH) expression/activity in TM4 Sertoli cells. Mol. Cell. Endocrinol. Jun 18 2016 [PMID: 27329155] (WB, Mouse) WB Mouse
Regueira M, Artagaveytia SL, Galardo MN et al. Novel molecular mechanisms involved in hormonal regulation of lactate production in Sertoli cells. Reproduction. 2015 Jul 29 [PMID: 26224098] (WB, Rat)

Details:
Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] antibody recognizing the phosphorylated form of PDC (P-PDC) was used for WB analysis of lysates from Sprague–Dawley rats' Sertoli cells that were stimulated in vitro with follicle-stimulating hormone or basic fibroblast growth factor (Figure 6).
WB Rat
Zuo RJ, Gu XW, Qi QR et al. Warburg-like glycolysis and lactate shuttle in female mouse decidua during early pregnancy J. Biol. Chem. 2015 Jul 15 [PMID: 26178372] (WB, Mouse)

Details:
p-Pdhe1-alpha [p Ser293] antibody used for Western blot analysis of primary mouse endometrial stromal cells.
WB Mouse
Hagopian K, Tomilov AA, Kim K et al. Key Glycolytic Enzyme Activities of Skeletal Muscle Are Decreased under Fed and Fasted States in Mice with Knocked Down Levels of Shc Proteins PLoS ONE 2015 Apr 17 [PMID: 25880638] (Mouse) Mouse
Show All 25 Publications.

Reviews for Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB110-93479:
Filter by Applications
WB
(4)
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Human
(2)
Mouse
(1)
Rat
(1)
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Images Ratings Applications Species Date Details
Western Blot Pyruvate Dehydrogenase E1-alpha subunit NB110-93479
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reviewed by:
Anonymous
WB Human 07/10/2018
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Summary

ApplicationWestern Blot
Sample Testedplacenta cells
SpeciesHuman
LotA1
Western Blot Pyruvate Dehydrogenase E1-alpha subunit NB110-93479
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reviewed by:
Anonymous
WB Mouse 05/12/2016
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Summary

ApplicationWestern Blot
Sample Testedprimary mouse macrophage lysates
SpeciesMouse

Blocking

Blocking Details5% Skim milk, 1h, room temperature

Primary Anitbody

Dilution Ratio1:1000, overnight, 4 deg, on a shaker

Secondary Antibody

Secondary DescriptionGoat anti-rabbit, peroxidase labeled
Secondary Concentration1:1000

Details

Detection NotesPierce Supersignal West Femto chemiluminiscent substrate, FluorChem HD instrument, 1-2 min exposure
Western Blot Pyruvate Dehydrogenase E1-alpha subunit NB110-93479
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reviewed by:
Silvina Meroni
WB Rat 01/14/2014
View

Summary

ApplicationWestern Blot
Sample Testedrat Sertoli cells
SpeciesRat
LotK-1

Blocking

Blocking Details1X TBS, 0.1% Tween-20 with 5% m/w nonfat dry milk, 3 hours at room temperature

Primary Anitbody

Dilution Ratio1:1000 in 1X TBS, 0.1% Tween-20, over night at 4ºC

Secondary Antibody

Secondary DescriptionAnti rabbit secondary antibody Conjugated to Horseradish Peroxidase
Secondary Manufacturer Cat#Cell Signalling Technology #7074
Secondary Concentration1:4000

Details

Detection NotesPhototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody CST#7071
Western Blot Pyruvate Dehydrogenase E1-alpha subunit NB110-93479
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reviewed by:
Alexandra Grassian
WB Human 08/12/2010
View

Summary

ApplicationWestern Blot
Sample TestedMCF10A whole cell lysate, Sample Amount: 30ug
SpeciesHuman
CommentsVery clean, strong signal. I highly recommend this antibody.

Blocking

Blocking DetailsBlocking Buffer: 2% BSA, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 2% BSA, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Alexa 680 goat anti-rabbit, Secondary Ab Dilution Ratio: 1:5000
Secondary Manufacturer Cat#A21109

Details

Detection NotesDetection Method: Odyssey imager, Exposure Time: level 5, Positive Control: none, Negative Control: None, Specific Bands: 40 kDa

Comments

CommentsVery clean, strong signal. I highly recommend this antibody.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-93479AF405
Alexa Fluor 488 Labeled NB110-93479AF488
Alexa Fluor 647 Labeled NB110-93479AF647
Alexa Fluor 700 Labeled NB110-93479AF700
Biotin Labeled NB110-93479B
DyLight 405 Labeled NB110-93479V
DyLight 488 Labeled NB110-93479G
DyLight 550 Labeled NB110-93479R
DyLight 650 Labeled NB110-93479C
FITC Labeled NB110-93479F
HRP Labeled NB110-93479H

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Bioinformatics Tool for Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479)

Discover related pathways, diseases and genes to Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479)

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Blogs on Pyruvate Dehydrogenase E1-alpha subunit.

Pyruvate Dehydrogenase E1-alpha subunit: Understanding the Catalysts in Glycolysis
Pyruvate Dehydrogenase E1-alpha subunit (PDHA1) is one of multiple enzymes in a mitochondrial complex, called the Pyruvate Dehydrogenase (PDH) complex, involved in the transition between glycolysis and the tricarboxylic acid (TCA) cycle during...  Read full blog post.

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Recent Reviews

5
5
4
4
0
3
0
2
0
1
0

Anonymous
07/10/2018
Application: WB
Species: Human

Anonymous
05/12/2016
Application: WB
Species: Mouse

Silvina Meroni
01/14/2014
Application: WB
Species: Rat

Bioinformatics

Gene Symbol PDHA1