Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free

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Biological Strategies: Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free [NB110-93479] - Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - MEF were ...read more
Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] antibody (1:250) was tested in HeLa cells with ...read more
Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Detection of PDHE1 alpha [S293] in an in vitro autophosphorylation of PDH complex in response to different stimulants: (-) none, ...read more
Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - PDH activity and protein levels of PDK4 and p-PDH in hindlimb skeletal muscle. Samples were resolved by electrophoresis, ...read more
Rate of aerobic glycolysis in the presence of BA.MEF were treated with 10 uM BA or DMSO (0.1%) for 16 h before they were subjected to a glycolysis stress test as described under "Materials and Methods". (D) MEF were ...read more
PDH activity and protein levels of PDK4 and p-PDH in hindlimb skeletal muscle.Skeletal muscle samples from WT and ShcKO mice, under fed and fasted conditions, were used to determine the activity of PDH (A), as described ...read more
PDK4 expression during syncytialization of human placental trophoblasts. (C) Changes in the phosphorylation of PDHE1alpha during syncytialization n = 4. Image collected and cropped by CiteAb from the following ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IP, WB, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free Summary

Immunogen
A synthetic peptide surrounding the phosphorylated serine 293 of the human Pyruvate Dehydrogenase E1-alpha subunit protein. [Swiss-Prot: #P08559]
Modification
p Ser293
Localization
Mitochondrion matrix
Specificity
This is specific for the phosphorylated Serine 293 form of the PDHE1 alpha protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDHA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry reported in scientific literature (PMID 31900478)
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence 1:50-1:250
  • Immunoprecipitation reported in scientific literature (PMID 27450723)
  • Knockout Validated reported in scientific literature (PMID 31742248)
  • Western Blot 1:1000-1:5000
Application Notes
In Western blot, a band is seen ~43 kDa. It is recommended that BSA rather than non-fat milk be used in membrane blocking and antibody dilution.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Pyruvate Dehydrogenase E1-alpha subunit Overexpression Lysate
Reviewed Applications
Read 5 Reviews rated 4.8
using
NB110-93479 in the following applications:

Publications
Read Publications using
NB110-93479 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free

  • EC 1.2.4.1
  • PDHA
  • PDHE1-A type I
  • PHE1APDHCE1A
  • pyruvate dehydrogenase (lipoamide) alpha 1
  • pyruvate dehydrogenase complex, E1-alpha polypeptide 1
  • pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Background

Pyruvate dehydrogenase (PDH) complex is localized to mitochondrial matrix, wherein it catalyzes the irreversible oxidative decarboxylation of pyruvate to generate acetyl-CoA, NADH, and CO2. The PDH complex contains three primary enzyme components: pyruvate dehydrogenase (E1 or PDHA- heterotetramer of two alpha- and two beta-subunits), dihydrolipoamide transacetylase (E2 or DLAT), and dihydrolipoamide dehydrogenase (E3 or DLD). The activity of PDH is controlled by specific E1 kinase and phospho-E1-phosphatase enzymes, which respectively inactivate and activate PDH complex by phosphorylation and dephosphorylation of E1alpha-subunit's serine residues. PDK family kinases mediated phosphorylation at Ser-293 blocks the access to active site leading to inactivation of the enzyme and vice versa. E3-binding protein (E3BP) is another component which is required for proper interaction of E2 and E3 components, and the overall complex contains 12 copies of E3, 30 copies of E1, 60 copies of E2, and 12 copies of E3BP. Acetyl-CoA generated gets utilized in TCA (citric acid/Krebs cycle) where it reacts with oxaloacetate to form citrate or it may also be used for biosynthesis of fatty acid or cholesterol. Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD), X-linked Leigh syndrome (X-LS) and it has also been implicated in ageing, glucose intolerance, cancer as well as Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-93479
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IP, WB, KO

Publications for Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479)(56)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: FLOW, IB, IF/IHC, IHC-Fr, IHC-FrFl, IP, KO, WB.


Filter By Application
FLOW
(2)
IB
(1)
IF/IHC
(1)
IHC-Fr
(1)
IHC-FrFl
(1)
IP
(2)
KO
(1)
WB
(39)
All Applications
Filter By Species
Human
(18)
Mouse
(21)
Rat
(5)
All Species
Showing Publications 1 - 10 of 56. Show All 56 Publications.
Publications using NB110-93479 Applications Species
Li Y, Cai J, Liu Y et al. CcpA-Knockout Staphylococcus aureus Induces Abnormal Metabolic Phenotype via the Activation of Hepatic STAT5/PDK4 Signaling in Diabetic Mice Pathogens 2023-10-30 [PMID: 38003764] (WB, Mouse) WB Mouse
Rossi SP, Matzkin ME, Riviere E et al. Melatonin improves oxidative state and lactate metabolism in rodent Sertoli cells Molecular and cellular endocrinology 2023-07-27 [PMID: 37516434] (WB, Human)

Details:
1:1000 WB dilution
WB Human
Bekeova C, Han JI, Xu H et al. Acyl-CoA thioesterase-2 facilitates ?-oxidation in glycolytic skeletal muscle in a lipid supply dependent manner bioRxiv : the preprint server for biology 2023-06-27 [PMID: 37425757] (WB, Mouse) WB Mouse
Li J, Zhu X, Oberdier MT et al. A cell-penetrating PHLPP peptide improves cardiac arrest survival in murine and swine models The Journal of clinical investigation 2023-05-01 [PMID: 37115695] (WB, Mouse) WB Mouse
Holloway C, Zhong G, Kim YK et al. Retinoic acid regulates pyruvate dehydrogenase kinase 4 (Pdk4) to modulate fuel utilization in the adult heart: Insights from wild-type and beta-carotene 9',10' oxygenase knockout mice FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022-09-01 [PMID: 36004605] (WB, Mouse) WB Mouse
Ito N, Takatsu A, Ito H et al. Slc12a8 in the lateral hypothalamus maintains energy metabolism and skeletal muscle functions during aging Cell reports 2022-07-26 [PMID: 35905718] (WB, Mouse)

Details:
1:4000 dilution
WB Mouse
Li J, Chang WT, Qin G et al. Baicalein Preconditioning Cardioprotection Involves Pro-Oxidant Signaling and Activation of Pyruvate Dehydrogenase The American journal of Chinese medicine 2022-06-23 [PMID: 35748215]
Martin A, Fernandez M, Cattaneo E et al. Type 1 Insulin-Like Growth Factor Receptor Nuclear Localization in High-Grade Glioma Cells Enhances Motility, Metabolism, and In Vivo Tumorigenesis Frontiers in Endocrinology [PMID: 35574033] (WB, Human) WB Human
Gatie M, Cooper T, Khazaee R et al. Lactate enhances mouse ES cell differentiation towards XEN cells in vitro Stem Cells 2022-03-24 [PMID: 35323987]
Damerau A, Kirchner M, Pfeiffenberger M et al. Metabolic reprogramming of synovial fibroblasts in osteoarthritis by inhibition of pathologically overexpressed pyruvate dehydrogenase kinases Metabolic engineering 2022-03-13 [PMID: 35296430] (FLOW, Human) FLOW Human
Show All 56 Publications.

Reviews for Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479) (5) 4.85

Average Rating: 4.8
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB110-93479:
Filter by Applications
WB
(5)
All Applications
Filter by Species
Human
(3)
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Pyruvate Dehydrogenase E1-alpha subunit NB110-93479
Enlarge
5
reviewed by:
Verified Customer
WB Human 07/10/2018
View

Summary

ApplicationWestern Blot
Sample Testedplacenta cells
SpeciesHuman
LotA1
Western Blot Pyruvate Dehydrogenase E1-alpha subunit NB110-93479
Enlarge
4
reviewed by:
Shahreen Amin
WB Human 06/12/2018
View

Summary

ApplicationWestern Blot
Sample TestedDifferentiated Neuronal NTERA2 cells,NTera-2 human testicular embryonic carcinoma cell line
SpeciesHuman
Lotp-1

Comments

CommentsThe site was similar in PDHA2 and we IP PDHA2 and its mutants at the site and probed with the antibody
Western Blot Pyruvate Dehydrogenase E1-alpha subunit NB110-93479
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 05/12/2016
View

Summary

ApplicationWestern Blot
Sample Testedprimary mouse macrophage lysates
SpeciesMouse
Western Blot Pyruvate Dehydrogenase E1-alpha subunit NB110-93479
Enlarge
5
reviewed by:
Silvina Meroni
WB Rat 01/14/2014
View

Summary

ApplicationWestern Blot
Sample Testedrat Sertoli cells
SpeciesRat
LotK-1
Western Blot Pyruvate Dehydrogenase E1-alpha subunit NB110-93479
Enlarge
5
reviewed by:
Alexandra Grassian
WB Human 08/12/2010
View

Summary

ApplicationWestern Blot
Sample TestedMCF10A whole cell lysate, Sample Amount: 30ug
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pyruvate Dehydrogenase E1-alpha subunit Antibody (NB110-93479) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Pyruvate Dehydrogenase E1-alpha subunit.

Pyruvate Dehydrogenase E1-alpha subunit: Understanding the Catalysts in Glycolysis
Pyruvate Dehydrogenase E1-alpha subunit (PDHA1) is one of multiple enzymes in a mitochondrial complex, called the Pyruvate Dehydrogenase (PDH) complex, involved in the transition between glycolysis and the tricarboxylic acid (TCA) cycle during...  Read full blog post.

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Recent Reviews

4.8
5
4
4
1
3
0
2
0
1
0

Verified Customer
07/10/2018
Application: WB
Species: Human

Shahreen Amin
06/12/2018
Application: WB
Species: Human

Verified Customer
05/12/2016
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PDHA1