| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1335SS | |||
| Azide and BSA Free | NBP2-80745 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-268SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1612-0.05ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-48336SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05198-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60531SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-717SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-11427SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1992SS | |||
![Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Ethanol feeding increases CHOP expression. Image from verified customer review.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Western-Blot-NB600-1335-img0011.jpg "Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Ethanol feeding increases CHOP expression. Image from verified customer review.")
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Immunohistochemistry-Paraffin-NB600-1335-img0010.jpg)
![Flow Cytometry: GADD153/CHOP Antibody (9C8) [NB600-1335] - An intracellular stain was performed on SK-MEL-28 cells with GADD153/CHOP Antibody (9C8) NB600-1335 (blue) and a matched isotype control MAB004 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Flow-Cytometry-NB600-1335-img0012.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - GADD153/CHOP expression in HeLa cells treated with 2.5 ug/mL tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Western-Blot-NB600-1335-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Analysis of endogenous CHOP/GADD153 from primary human fibroblasts using NB600-1335. Lane 1: Untreated cells, Lane 2: Cells treated with tunicamycin for 10 hours.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Western-Blot-NB600-1335-img0004.jpg)
![Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Analysis of CHOP in rat heart tissue lysate. Image courtesy of product review submitted by Lee Hsiao-Wei.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Western-Blot-NB600-1335-img0005.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Immunohistochemistry-Paraffin-NB600-1335-img0008.jpg)
![Simple Western: GADD153/CHOP Antibody (9C8) [NB600-1335] - Image shows a specific band for CHOP/GADD153 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Simple-Western-NB600-1335-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] - hPDLSCs-derived neural-like cells are connected by synapse-like interactions. hPDLSCs-derived neural-like cells connect to one another. Synapse-associated protein Synapsin1 is found in the cell membrane of hPDLSCs-derived neural-like cells at the neurite contact areas. Scale bar: 25 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-54745-3) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-Immunocytochemistry-Immunofluorescence-NB300-104-img0013.jpg "Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] - hPDLSCs-derived neural-like cells are connected by synapse-like interactions. hPDLSCs-derived neural-like cells connect to one another. Synapse-associated protein Synapsin1 is found in the cell membrane of hPDLSCs-derived neural-like cells at the neurite contact areas. Scale bar: 25 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-54745-3) licensed under a CC-BY license.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] - Characterization of WT and slow ESCs differentiated to neurons. Immunofluorescence staining for neuronal markers Map2, and Syn1 in neurons cultured on poly-ornithine-/laminin-coated plates. Expression of neuronal markers in neurons cultured on poly-ornithine-/laminin-coated plates from RNA-seq analysis, n = 3. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/embj.2018101244) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-Immunocytochemistry-Immunofluorescence-NB300-104-img0014.jpg)
![Western Blot: Synapsin I Antibody [NB300-104] - Western blot analysis showing induced expression of neuronal functional marker Synapsin I at protein levels in MNC (meningial neuronal construct). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30443545/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Synapsin I Antibody-Western Blot-NB300-104-img0015.jpg)
![Immunohistochemistry-Paraffin: Synapsin I Antibody [NB300-104] - Human cerebellum section, formalin fixed paraffin embedded. Stained with Synpasin-1 at 1:250. Citrate antigen retrieval at pH 6. Image from verified customer review.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-Immunohistochemistry-Paraffin-NB300-104-img0012.jpg)
![Western Blot: Synapsin I Antibody [NB300-104] - 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~78k synapsin I doublet protein.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-Western-Blot-NB300-104-img0007.jpg)
![Western Blot: Synapsin I Antibody [NB300-104] - 10 ug of rat brain lysate showing specific immunolabeling of the ~78 kDa Synapsin I doublet.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-Western-Blot-NB300-104-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] - ICC-IF validation analysis of Synapsin 1 Antibody on cultured rat caudate neurons. This representative image shows the punctate distribution of Synapsin 1 (green) and MAP (red) proteins in a neuronal cell.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-Immunocytochemistry-Immunofluorescence-NB300-104-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104] - Characterization of WT & slow ESCs differentiated to neurons (related to Fig 4)Immunofluorescence staining for neuronal markers Map2, Syn1, & NeuN in neurons cultured on poly‐ornithine‐/laminin‐coated plates.Expression of neuronal markers in neurons cultured on poly‐ornithine‐/laminin‐coated plates from RNA‐seq analysis, n = 3. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30988016), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-104_rabbit-polyclonal-synapsin-i-antibody-310202415394528.jpg)
![Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104] - hPDLSCs-derived neural-like cells are connected by synapse-like interactions. hPDLSCs-derived neural-like cells connect to one another (a) through different types of synapses-like interactions, including dendrodendritic-like, axoaxonic-like & axodendritic-like synapses (b). (c) Synapse-associated proteins Cx43, Synaptophysin & Synapsin1 are found in the cell membrane of hPDLSCs-derived neural-like cells at the neurite contact areas. Scale bar: 25 μm. AA, axoaxonic-like synapse; AD, axodendritic-like synapse; DD, dendrodendritic-like synapse; LM, light microscopy; SEM, scanning electron microscopy. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31792338), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-104_rabbit-polyclonal-synapsin-i-antibody-310202415371980.jpg)
![Western Blot: Synapsin I Antibody [NB300-104] - Synaptic localization of TA at the PC–PF synaptic contacts.Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein (PSD95) & Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) & TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C–E) The high magnification of the inset in B is reproduced in different merge images & by the relative split channels. C) Merge image of Cb & V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D) Merge image of TA & Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E) Merge image of TA & V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) & TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23840813), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-104_rabbit-polyclonal-synapsin-i-antibody-31020241621237.jpg)
![Western Blot: Synapsin I Antibody [NB300-104] - Tau oligomers induce synaptic dysfunction. (A) Representative Western blot of mouse hippocampus homogenate. The levels of synaptophysin, synapsin-1, & septin-11 were measured by band quantification & normalized with the levels of tubulin. PBSo indicates representative bands of hippocampal area injected with PBS in mice also injected with tau oligomers, PBSf indicates PBS injection in mice also injected with tau fibrils, & PBSm indicates PBS injection in mice also injected with tau monomers. (B) Synaptophysin levels were significantly lower in the hemispheres injected with tau oligomers in comparison with the ones injected with fibrils, monomers, or PBS. (C) No significant differences in the levels of synapsin-1 were observed. (D) Only the hemisphere injected with tau oligomers presents a decrease in the level of septin-11. Data are represented as the mean ± SE. *p < 0.01, n = 6 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21645391), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-104_rabbit-polyclonal-synapsin-i-antibody-31020241620516.jpg)
![Western Blot: LRRK2 Antibody [NB300-268] - Western blotting of LRRK2. Recombinant LRRK2 (arrowhead) from transfected (+) HEK 293T and M17 cells was specifically recognized by all four LRRK2 antibodies (Ab1, Ab2, Ab3, and Ab4) used in this study. LRRK2 was not recognized in non-transfected cells (-). Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-1-17), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Western-Blot-NB300-268-img0023.jpg "<b>Independent Antibodies Validation. </b>Western Blot: LRRK2 Antibody [NB300-268] - Western blotting of LRRK2. Recombinant LRRK2 (arrowhead) from transfected (+) HEK 293T and M17 cells was specifically recognized by all four LRRK2 antibodies (Ab1, Ab2, Ab3, and Ab4) used in this study. LRRK2 was not recognized in non-transfected cells (-). Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-1-17), licensed under a CC-BY license.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Frozen: LRRK2 Antibody [NB300-268] - Staining as described in PMID 24312256. Image from verified customer review.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Immunohistochemistry-Frozen-NB300-268-img0014.jpg)
![Western Blot: LRRK2 Antibody [NB300-268] - LRRK2 N-terminal sequences show increased aggregation. Western blots of cell lysates of SH-SY5Y cells transfected with indicated LRRK2 construct showed equal expression of the LRRK2 protein with anti-HA (top panel) or anti-C-terminal- (bottom panel) LRRK2 antibody. Equal lysate loading is indicated by Beta-actin loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045149) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Western-Blot-NB300-268-img0028.jpg)
![Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - Specificity of immunostaining for LRRK2. Specificity of the antibodies used for immunocytochemistry was determined by performing adsorptions with the corresponding peptide sequences. Immunochemical localization of LBs (arrows) by Ab1 against LRRK2900-100 (left) was blocked using respective peptide antigens (right). Adjacent sections with LB within pigmented neurons from the substantia nigra of a case of PD were used. Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-1-17), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Immunohistochemistry-Paraffin-NB300-268-img0026.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - Immunocytochemistry of LRRK2 in PD using antibodies (Ab1, Ab4) raised against sequences corresponding to various regions shown on the schematic diagram of LRRK2 (red bars) were used on brainstem sections of PD. Scale bars: 10 um (top); E-L = 100 um (bottom). Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-1-17), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Immunohistochemistry-Paraffin-NB300-268-img0025.jpg)
![Flow Cytometry: LRRK2 Antibody [NB300-268] - Analysis using the Alexa Fluor 647 conjugate of NB300-268. Staining of LRRK2 in normal human peripheral blood cells (anticoagulated Lithium-Heparin) using anti-LRRK2 antibody conjugated with AF647. The primary antibody was used at a dilution of 1:200 and incubated for 20 min at room temperature. Image from verified customer review.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Flow-Cytometry-NB300-268-img0019.jpg)
![Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - LRRK2 accumulates in globules in alphaS tg mice. Triple immunofluorescence for alphaS, LRRK2 and Rab5B for basal ganglia in alphaS tg mice. LRRK2 and Rab5B were colocalized in axon terminal (arrow), but were not colocalized in the alphaS-globule (arrowhead) Scale bar = 10 um for all panels. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-34), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Immunohistochemistry-Paraffin-NB300-268-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NB300-268] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-LRRK2 Antibody NB300-268 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Immunocytochemistry-Immunofluorescence-NB300-268-img0031.jpg)
![Flow Cytometry: LRRK2 Antibody [NB300-268] - An intracellular stain was performed on U2OS cells with LRRK2 Antibody NB300-268 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/LRRK2-Antibody-Flow-Cytometry-NB300-268-img0030.jpg)
![Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NB300-268] - LRRK2 antibody was tested in SH-SY-5Y cells at a 1:200 dilution against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red), and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Immunocytochemistry-Immunofluorescence-NB300-268-img0015.jpg)
![Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - Staining of human brain, Putamen, Neurons and Glia using a 60X magnification.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Immunohistochemistry-Paraffin-NB300-268-img0020.jpg)
![Flow Cytometry: LRRK2 Antibody [NB300-268] - An intracellular stain was performed on U2OS cells with LRRK Antibody NB300-268AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Flow-Cytometry-NB300-268-img0022.jpg)
![Flow Cytometry: LRRK2 Antibody [NB300-268] - An intracellular stain was performed on Neuro2a cells with LRRK2 Antibody NB300-268 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/LRRK2-Antibody-Flow-Cytometry-NB300-268-img0029.jpg)
![Western Blot: LRRK2 Antibody - BSA Free [NB300-268] - Western blotting of LRRK2. A. Recombinant LRRK2 (arrowhead) from transfected (+) HEK 293T & M17 cells was specifically recognized by all four LRRK2 antibodies (Ab1, Ab2, Ab3, & Ab4) used in this study. LRRK2 was not recognized in non-transfected cells (-). B. Recombinant LRRK2 (arrowhead) from transfected M17 cells is detected by anti-LRRK2 Ab4 (lane 1), but not after absorption of the antibody with its peptide antigen (lane 2). Brain LRRK2 was recognized by anti-LRRK2 Ab4 in two controls (lanes 3 & 4) & two PD cases (lanes 5 & 6). Cell lysates (10 μg protein) & brain homogenates (100 μg protein) were prepared & loaded on 6% SDS-PAGE gels for Western blot analysis using anti-LRRK2 antibodies Ab1–Ab4 in (A) & Ab4 in (B). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-310202415371926.jpg)
![Flow Cytometry: LRRK2 Antibody - BSA Free [NB300-268] - Deletion of LRRK2 attenuates Mn-induced apoptosis & cell death in RAW 264.7 cells.(A) LRRK2 WT or KO RAW 264.7 cells were treated with Mn (250 μM) for the designated times, followed by flow cytometry analysis to determine Mn-induced apoptosis. Both early & late apoptotic cells (Q2 & Q3) were measured. (B) At the end of Mn exposure, cell viability was determined by MTT assay. @@@, p < 0.001; ###, p < 0.001; ***, p < 0.001 compared to the control (one-way ANOVA followed by Tukey’s post hoc test; n = 3, for apoptosis assay; n = 6, for MTT assay). The data shown are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30645642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-31020241539739.jpg)
![Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody - BSA Free [NB300-268] - LRRK2 accumulates in globules in alpha S tg mice. (a & b) Double immunofluorescence for alpha S with parkin, PINK1, DJ-1, LRRK2, or negative control (the immunopeptide-preabsorbed anti-LRRK2 antibody) in alpha S tg mice (a) & P123H beta S tg mice (b). Note that alpha S-globules were immunopositive for LRRK2 (~79%, n = 22), whereas P123H beta S globules were negative for LRRK2. Representative images are shown for the thalamus ( alpha S) & basal ganglia (P123H beta S). Scale bar = 5 μm for all panels. (c) Triple immunofluorescence for alpha S, LRRK2 & Rab5B for basal ganglia in alpha S tg mice. LRRK2 & Rab5B were colocalized in axon terminal (arrow), but were not colocalized in the alpha S-globule (arrowhead) Scale bar = 10 μm for all panels. Image collected & cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-31020241538242.jpg)
![Immunohistochemistry-Paraffin: LRRK2 Antibody - BSA Free [NB300-268] - Specificity of immunostaining for LRRK2. Specificity of the antibodies used for immunocytochemistry was determined by performing adsorptions with the corresponding peptide sequences. Immunochemical localization of LBs (arrows) by Ab4 against LRRK22500–2527 (A) & Ab1 against LRRK2900–100 (C) was blocked using respective peptide antigens (B & D). Adjacent sections with LB within pigmented neurons from the substantia nigra of a case of PD were used. Scale bar: 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-310202415392599.jpg)
![Immunohistochemistry-Paraffin: LRRK2 Antibody - BSA Free [NB300-268] - Immunocytochemistry of LRRK2 in DLB. Cortical LBs (arrows) in DLB were positive for LRRK2 using both Ab4 against LRRK22500–2527 (A), & Ab1 against LRRK2900–100 (B). Neuronal cytoplasm (C) was also strongly labelled throughout the cortex by Ab4 & with a consistently more granular pattern by Ab1 (inset in C) in cases of DLB. Control cases display similar, though less intense neuronal labeling (D). Biochemically purified cortical LBs were strongly positive for staining by Ab4 (E) as well as by anti-alpha -synuclein (F), while they were unstained when omitting primary antibody (G). Large vessels (H) were also intensely labelled by Ab4. Scale bars: A, B = 25 μm; C, D = 100 μm; E-G = 25 μm; H = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-310202415304221.jpg)
![Immunohistochemistry-Paraffin: LRRK2 Antibody - BSA Free [NB300-268] - Immunocytochemistry of LRRK2 in DLB. Cortical LBs (arrows) in DLB were positive for LRRK2 using both Ab4 against LRRK22500–2527 (A), & Ab1 against LRRK2900–100 (B). Neuronal cytoplasm (C) was also strongly labelled throughout the cortex by Ab4 & with a consistently more granular pattern by Ab1 (inset in C) in cases of DLB. Control cases display similar, though less intense neuronal labeling (D). Biochemically purified cortical LBs were strongly positive for staining by Ab4 (E) as well as by anti-alpha -synuclein (F), while they were unstained when omitting primary antibody (G). Large vessels (H) were also intensely labelled by Ab4. Scale bars: A, B = 25 μm; C, D = 100 μm; E-G = 25 μm; H = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-31020241534387.jpg)
![Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody - BSA Free [NB300-268] - LRRK2 accumulates in globules in alpha S tg mice. (a & b) Double immunofluorescence for alpha S with parkin, PINK1, DJ-1, LRRK2, or negative control (the immunopeptide-preabsorbed anti-LRRK2 antibody) in alpha S tg mice (a) & P123H beta S tg mice (b). Note that alpha S-globules were immunopositive for LRRK2 (~79%, n = 22), whereas P123H beta S globules were negative for LRRK2. Representative images are shown for the thalamus ( alpha S) & basal ganglia (P123H beta S). Scale bar = 5 μm for all panels. (c) Triple immunofluorescence for alpha S, LRRK2 & Rab5B for basal ganglia in alpha S tg mice. LRRK2 & Rab5B were colocalized in axon terminal (arrow), but were not colocalized in the alpha S-globule (arrowhead) Scale bar = 10 μm for all panels. Image collected & cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-31020241517133.jpg)
![Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody - BSA Free [NB300-268] - LRRK2 accumulates in globules in alpha S tg mice. (a & b) Double immunofluorescence for alpha S with parkin, PINK1, DJ-1, LRRK2, or negative control (the immunopeptide-preabsorbed anti-LRRK2 antibody) in alpha S tg mice (a) & P123H beta S tg mice (b). Note that alpha S-globules were immunopositive for LRRK2 (~79%, n = 22), whereas P123H beta S globules were negative for LRRK2. Representative images are shown for the thalamus ( alpha S) & basal ganglia (P123H beta S). Scale bar = 5 μm for all panels. (c) Triple immunofluorescence for alpha S, LRRK2 & Rab5B for basal ganglia in alpha S tg mice. LRRK2 & Rab5B were colocalized in axon terminal (arrow), but were not colocalized in the alpha S-globule (arrowhead) Scale bar = 10 μm for all panels. Image collected & cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-310202415304292.jpg)
![Western Blot: LRRK2 Antibody - BSA Free [NB300-268] - Western blotting of LRRK2. A. Recombinant LRRK2 (arrowhead) from transfected (+) HEK 293T & M17 cells was specifically recognized by all four LRRK2 antibodies (Ab1, Ab2, Ab3, & Ab4) used in this study. LRRK2 was not recognized in non-transfected cells (-). B. Recombinant LRRK2 (arrowhead) from transfected M17 cells is detected by anti-LRRK2 Ab4 (lane 1), but not after absorption of the antibody with its peptide antigen (lane 2). Brain LRRK2 was recognized by anti-LRRK2 Ab4 in two controls (lanes 3 & 4) & two PD cases (lanes 5 & 6). Cell lysates (10 μg protein) & brain homogenates (100 μg protein) were prepared & loaded on 6% SDS-PAGE gels for Western blot analysis using anti-LRRK2 antibodies Ab1–Ab4 in (A) & Ab4 in (B). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-31020241536376.jpg)
![Immunohistochemistry-Paraffin: LRRK2 Antibody - BSA Free [NB300-268] - Immunocytochemistry of LRRK2 in DLB. Cortical LBs (arrows) in DLB were positive for LRRK2 using both Ab4 against LRRK22500–2527 (A), & Ab1 against LRRK2900–100 (B). Neuronal cytoplasm (C) was also strongly labelled throughout the cortex by Ab4 & with a consistently more granular pattern by Ab1 (inset in C) in cases of DLB. Control cases display similar, though less intense neuronal labeling (D). Biochemically purified cortical LBs were strongly positive for staining by Ab4 (E) as well as by anti-alpha -synuclein (F), while they were unstained when omitting primary antibody (G). Large vessels (H) were also intensely labelled by Ab4. Scale bars: A, B = 25 μm; C, D = 100 μm; E-G = 25 μm; H = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-310202415175254.jpg)
![Immunohistochemistry-Paraffin: LRRK2 Antibody - BSA Free [NB300-268] - Immunocytochemistry of LRRK2 in DLB. Cortical LBs (arrows) in DLB were positive for LRRK2 using both Ab4 against LRRK22500–2527 (A), & Ab1 against LRRK2900–100 (B). Neuronal cytoplasm (C) was also strongly labelled throughout the cortex by Ab4 & with a consistently more granular pattern by Ab1 (inset in C) in cases of DLB. Control cases display similar, though less intense neuronal labeling (D). Biochemically purified cortical LBs were strongly positive for staining by Ab4 (E) as well as by anti-alpha -synuclein (F), while they were unstained when omitting primary antibody (G). Large vessels (H) were also intensely labelled by Ab4. Scale bars: A, B = 25 μm; C, D = 100 μm; E-G = 25 μm; H = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-310202415345320.jpg)
![Immunohistochemistry-Paraffin: LRRK2 Antibody - BSA Free [NB300-268] - Immunocytochemistry of LRRK2 in DLB. Cortical LBs (arrows) in DLB were positive for LRRK2 using both Ab4 against LRRK22500–2527 (A), & Ab1 against LRRK2900–100 (B). Neuronal cytoplasm (C) was also strongly labelled throughout the cortex by Ab4 & with a consistently more granular pattern by Ab1 (inset in C) in cases of DLB. Control cases display similar, though less intense neuronal labeling (D). Biochemically purified cortical LBs were strongly positive for staining by Ab4 (E) as well as by anti-alpha -synuclein (F), while they were unstained when omitting primary antibody (G). Large vessels (H) were also intensely labelled by Ab4. Scale bars: A, B = 25 μm; C, D = 100 μm; E-G = 25 μm; H = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-310202415384173.jpg)
![Western Blot: LRRK2 Antibody - BSA Free [NB300-268] - LRRK2 deletion attenuates Mn-induced pro-apoptotic protein levels.(A) After cells (LRRK2 WT & LRRK2 KO) were exposed to Mn (250 μM) for up to 24 h, total protein was extracted & followed by western blot analysis to determine protein levels of Bax & Daxx. beta -actin was used as a loading control. (B) The expression levels of Bax & Daxx modulated by Mn were quantified relative to its control levels in LRRK2 WT & KO cells. **, p < 0.01; ***, p < 0.001 (one-way ANOVA followed by Tukey’s post hoc test; n = 3). The data shown are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30645642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-31020241535611.jpg)
![Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody - BSA Free [NB300-268] - The interaction & phosphorylation of LRRK2 & LC1. (A) Cellular co-localisation of endogenous LRRK2 & MAP1B (LC1). SKNSH cells were labelled with LRRK2 (green) & LC1 (red) antibodies & signals were detected by immunofluorescence. Detailed figure in Additional file 1. (B) LRRK2 kinase assay. The LRRK2 wild-typed (WT) kinase interacted & phosphorylated the LC1 domain unlike the LRRK2 kinase-dead protein. (C) Western blotting of LRRK2 & LC1 proteins after a kinase assay. Both LRRK2 & LC1 proteins were probed for phospho-threonine & phospho-serine signals respectively. Image collected & cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-7-29), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-310202415382414.jpg)
![Western Blot: LRRK2 Antibody - BSA Free [NB300-268] - Mn increases LRRK2 kinase activity & expression in RAW 264.7 & HMC3 cells.(A) Two-hundred (200) μg of protein were collected from cell lysates, followed by western blot analysis to determine the presence of LRRK2 in LRRK2 WT, KO RAW 264.7 & HMC3 cells. beta -actin was used as a loading control. (B,C) LRRK2 WT or HMC3 cells were treated with Mn (250 μM) for the designated times, followed by protein extraction & western blot analysis as described in the Methods section. Protein levels of LRRK2 & phosphorylated LRRK2 (S1292) in LRRK2 WT RAW 264.7 (B) & HMC3 (C) cells were quantified. (D,E) Effect of Mn on LRRK2 mRNA levels in LRRK2 WT RAW 264.7 (D) & HMC3 (E) cells were assessed as described in the Methods section. GAPDH was used as a loading control. (F) After pre-treatment with LRRK2 inhibitors GSK (1 μM) & MLi-2 (50 nM) for 90 min, RAW 264.7 cells were exposed to Mn (250 μM) for 20 min, followed by western blot analysis to detect phosphorylation of LRRK2 (S1292). ###, p < 0.001; *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared to the control (one-way ANOVA followed by Tukey’s post hoc test; n = 3). The data shown are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30645642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-31020241621814.jpg)
![Immunohistochemistry-Paraffin: LRRK2 Antibody - BSA Free [NB300-268] - Immunocytochemistry of LRRK2 in PD. Four antibodies raised against sequences corresponding to various regions shown on the schematic diagram of LRRK2 (red bars) were used on brainstem sections of PD (A-H) & age-matched controls (I-L). Intense immunolabeling of brainstem LBs in cases of PD was seen with Ab1 against LRRK2900–100 (A) & Ab4 against LRRK22500–2527(D). Both rim (A & D) & core (inset in D) of LBs were recognized. In contrast, LBs were not labelled in any case using antibodies directed against LRRK21246–1265 (Ab2, B) or LRRK21838–2133 (Ab3, C), for which the antigenic sites are located within the folded domains. The cell bodies of both pigmented & no-pigmented neurons (arrows) as well as axons (arrowheads) contain LRRK2, seen only using antibodies against sites outside the folded domains (Ab1, E & Ab4, H). In contrast, Ab4 staining was much less intense in control tissue (L). The muscle layer of both large & small vessels was consistently found to contain high levels of LRRK2 in almost all PD cases (seen in E, G, & H) & strikingly the vessels are the only structure immunolabeled with Ab3 recognizing LRRK21838–2133 in all cases studied (G & K). Ab2 to LRRK21246–1265 did not recognize LBs, cell bodies or vessels in any case (B, F, & J). Scale bars: A-D & inset = 10 μm; E-L = 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-310202416205168.jpg)
![Immunohistochemistry-Paraffin: LRRK2 Antibody - BSA Free [NB300-268] - Immunocytochemistry of LRRK2 in PD. Four antibodies raised against sequences corresponding to various regions shown on the schematic diagram of LRRK2 (red bars) were used on brainstem sections of PD (A-H) & age-matched controls (I-L). Intense immunolabeling of brainstem LBs in cases of PD was seen with Ab1 against LRRK2900–100 (A) & Ab4 against LRRK22500–2527(D). Both rim (A & D) & core (inset in D) of LBs were recognized. In contrast, LBs were not labelled in any case using antibodies directed against LRRK21246–1265 (Ab2, B) or LRRK21838–2133 (Ab3, C), for which the antigenic sites are located within the folded domains. The cell bodies of both pigmented & no-pigmented neurons (arrows) as well as axons (arrowheads) contain LRRK2, seen only using antibodies against sites outside the folded domains (Ab1, E & Ab4, H). In contrast, Ab4 staining was much less intense in control tissue (L). The muscle layer of both large & small vessels was consistently found to contain high levels of LRRK2 in almost all PD cases (seen in E, G, & H) & strikingly the vessels are the only structure immunolabeled with Ab3 recognizing LRRK21838–2133 in all cases studied (G & K). Ab2 to LRRK21246–1265 did not recognize LBs, cell bodies or vessels in any case (B, F, & J). Scale bars: A-D & inset = 10 μm; E-L = 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-310202416205158.jpg)
![Western Blot: LRRK2 Antibody - BSA Free [NB300-268] - Mn increases LRRK2 kinase activity & expression in RAW 264.7 & HMC3 cells.(A) Two-hundred (200) μg of protein were collected from cell lysates, followed by western blot analysis to determine the presence of LRRK2 in LRRK2 WT, KO RAW 264.7 & HMC3 cells. beta -actin was used as a loading control. (B,C) LRRK2 WT or HMC3 cells were treated with Mn (250 μM) for the designated times, followed by protein extraction & western blot analysis as described in the Methods section. Protein levels of LRRK2 & phosphorylated LRRK2 (S1292) in LRRK2 WT RAW 264.7 (B) & HMC3 (C) cells were quantified. (D,E) Effect of Mn on LRRK2 mRNA levels in LRRK2 WT RAW 264.7 (D) & HMC3 (E) cells were assessed as described in the Methods section. GAPDH was used as a loading control. (F) After pre-treatment with LRRK2 inhibitors GSK (1 μM) & MLi-2 (50 nM) for 90 min, RAW 264.7 cells were exposed to Mn (250 μM) for 20 min, followed by western blot analysis to detect phosphorylation of LRRK2 (S1292). ###, p < 0.001; *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared to the control (one-way ANOVA followed by Tukey’s post hoc test; n = 3). The data shown are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30645642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-310202416205187.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: LRRK2 Antibody - BSA Free [NB300-268] - Mn increases LRRK2 kinase activity & expression in RAW 264.7 & HMC3 cells.(A) Two-hundred (200) μg of protein were collected from cell lysates, followed by western blot analysis to determine the presence of LRRK2 in LRRK2 WT, KO RAW 264.7 & HMC3 cells. beta -actin was used as a loading control. (B,C) LRRK2 WT or HMC3 cells were treated with Mn (250 μM) for the designated times, followed by protein extraction & western blot analysis as described in the Methods section. Protein levels of LRRK2 & phosphorylated LRRK2 (S1292) in LRRK2 WT RAW 264.7 (B) & HMC3 (C) cells were quantified. (D,E) Effect of Mn on LRRK2 mRNA levels in LRRK2 WT RAW 264.7 (D) & HMC3 (E) cells were assessed as described in the Methods section. GAPDH was used as a loading control. (F) After pre-treatment with LRRK2 inhibitors GSK (1 μM) & MLi-2 (50 nM) for 90 min, RAW 264.7 cells were exposed to Mn (250 μM) for 20 min, followed by western blot analysis to detect phosphorylation of LRRK2 (S1292). ###, p < 0.001; *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared to the control (one-way ANOVA followed by Tukey’s post hoc test; n = 3). The data shown are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30645642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-31020241621239.jpg)
![Western Blot: LRRK2 Antibody - BSA Free [NB300-268] - Mn increases LRRK2 kinase activity & expression in RAW 264.7 & HMC3 cells.(A) Two-hundred (200) μg of protein were collected from cell lysates, followed by western blot analysis to determine the presence of LRRK2 in LRRK2 WT, KO RAW 264.7 & HMC3 cells. beta -actin was used as a loading control. (B,C) LRRK2 WT or HMC3 cells were treated with Mn (250 μM) for the designated times, followed by protein extraction & western blot analysis as described in the Methods section. Protein levels of LRRK2 & phosphorylated LRRK2 (S1292) in LRRK2 WT RAW 264.7 (B) & HMC3 (C) cells were quantified. (D,E) Effect of Mn on LRRK2 mRNA levels in LRRK2 WT RAW 264.7 (D) & HMC3 (E) cells were assessed as described in the Methods section. GAPDH was used as a loading control. (F) After pre-treatment with LRRK2 inhibitors GSK (1 μM) & MLi-2 (50 nM) for 90 min, RAW 264.7 cells were exposed to Mn (250 μM) for 20 min, followed by western blot analysis to detect phosphorylation of LRRK2 (S1292). ###, p < 0.001; *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared to the control (one-way ANOVA followed by Tukey’s post hoc test; n = 3). The data shown are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30645642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-31020241621810.jpg)
![Western Blot: LRRK2 Antibody - BSA Free [NB300-268] - LRRK2 kinase inhibition blocks Tat-induced S935 phosphorylation & inflammatory cytokine expression in BV-2 cells. (A) Western blot depicting pS935-LRRK2 & total LRRK2 protein levels 12 hours after saline or Tat-treatment ± LRRK2 kinase inhibition (LRRK2i). (B) Tat treatment significantly increased pS935-LRRK2, which was attenuated by LRRK2i. (C) We measured TNF-alpha , IL-6, MCP-1 & IL-10 mRNA levels by qRT-PCR 12 hours post saline or Tat treatment ± LRRK2i. LRRK2i attenuated Tat-induced TNF-alpha , IL-6, MCP-1 & IL-10 expression for all groups (*P <0.05, ***P <0.001, one way ANOVA, Newman-Keuls post-test). ANOVA, analysis of variance; LRRK2, leucine-rich repeat kinase2; pS935, phosphorylation of serine 935; Tat, trans activator of transcription. Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-9-261), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-310202416212357.jpg)
![Immunohistochemistry-Paraffin: LRRK2 Antibody - BSA Free [NB300-268] - Immunocytochemistry of LRRK2 in PD. Four antibodies raised against sequences corresponding to various regions shown on the schematic diagram of LRRK2 (red bars) were used on brainstem sections of PD (A-H) & age-matched controls (I-L). Intense immunolabeling of brainstem LBs in cases of PD was seen with Ab1 against LRRK2900–100 (A) & Ab4 against LRRK22500–2527(D). Both rim (A & D) & core (inset in D) of LBs were recognized. In contrast, LBs were not labelled in any case using antibodies directed against LRRK21246–1265 (Ab2, B) or LRRK21838–2133 (Ab3, C), for which the antigenic sites are located within the folded domains. The cell bodies of both pigmented & no-pigmented neurons (arrows) as well as axons (arrowheads) contain LRRK2, seen only using antibodies against sites outside the folded domains (Ab1, E & Ab4, H). In contrast, Ab4 staining was much less intense in control tissue (L). The muscle layer of both large & small vessels was consistently found to contain high levels of LRRK2 in almost all PD cases (seen in E, G, & H) & strikingly the vessels are the only structure immunolabeled with Ab3 recognizing LRRK21838–2133 in all cases studied (G & K). Ab2 to LRRK21246–1265 did not recognize LBs, cell bodies or vessels in any case (B, F, & J). Scale bars: A-D & inset = 10 μm; E-L = 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17137507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-31020241621837.jpg)
![Flow Cytometry: LRRK2 Antibody - BSA Free [NB300-268] - Inhibition of LRRK2 kinase activity attenuates Mn-induced apoptosis.(A) After pre-treatment with GSK (1 μM) for 90 min, cells (HMC3) were exposed to Mn (250 μM) for the designated time periods, followed by annexin V & PI staining & flow cytometry analysis to determine apoptosis. Early & late apoptotic cells (Q2 & Q3) were analyzed. (B,C) After pre-treatment with GSK (1 μM) for 90 min, cells (LRRK2 WT RAW 264.7 & HMC3) were exposed to Mn for designated time periods, followed by the MTT assay to determine cell viability, as described in the Methods section, (@@@, p < 0.001; *, p < 0.05; ***, p < 0.001 compared to the control (one-way ANOVA followed by Tukey’s post hoc test; n = 3, apoptosis assay; n = 6, MTT assay). The data shown are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30645642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-268_rabbit-polyclonal-lrrk2-antibody-31020241621844.jpg)
![Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) and S100beta as astrocytic protein marker proteins in the hippocampal area. Western blot images using GFAP, tubulin beta-3, and beta-actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized GFAP/beta-tubulin ratio by beta-actin levels relative to young adult wild-type C57BL/6J. White and black bars indicate the wild-type C57BL/6J and Tet-mev-1 mice, respectively. Data are expressed as mean +/- SD; *P 12 in each group. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12523), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-Western-Blot-NB100-1612-img0006.jpg "Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) and S100beta as astrocytic protein marker proteins in the hippocampal area. Western blot images using GFAP, tubulin beta-3, and beta-actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized GFAP/beta-tubulin ratio by beta-actin levels relative to young adult wild-type C57BL/6J. White and black bars indicate the wild-type C57BL/6J and Tet-mev-1 mice, respectively. Data are expressed as mean +/- SD; *P 12 in each group. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12523), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody [NB100-1612] - Beta-III Tubulin in rat primary motor neurons. Ab dilution 1:1000 in PBST (0.1% Triton X-100) + 10% GS O/N at 4C. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1612-img0011.jpg)
![Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) and S100beta as astrocytic protein marker proteins in the hippocampal area. Western blot images using S100beta, tubulin beta-3, and beta-actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized S100beta/beta-tubulin ratio by beta-actin levels relative to young adult wild-type C57BL/6J. White and black bars indicate the wild-type C57BL/6J and Tet-mev-1 mice, respectively. Data are expressed as mean +/- SD; *P 12 in each group. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12523), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-Western-Blot-NB100-1612-img0007.jpg)
![Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) & S100 beta as astrocytic protein marker proteins in the hippocampal area. (A) Western blot images using GFAP, tubulin beta‐3, & beta ‐actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized GFAP/ beta ‐tubulin ratio by beta ‐actin levels relative to young adult wild‐type C57BL/6J. White & black bars indicate the wild‐type C57BL/6J & Tet‐mev‐1 mice, respectively. Data are expressed as mean ± SD; *P 12 in each group. (B) Western blot images using S100 beta , tubulin beta‐3, & beta ‐actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized S100 beta / beta ‐tubulin ratio by beta ‐actin levels relative to young adult wild‐type C57BL/6J. White & black bars indicate the wild‐type C57BL/6J & Tet‐mev‐1 mice, respectively. Data are expressed as mean ± SD; *P 12 in each group. (C) Micrographs of immunohistochemical analysis on paraffined hippocampal tissue sections using GFAP & S100 beta antibody. Brown cells indicate GFAP‐stained astrocytes. Scale bar = 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27623715), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1612_chicken-polyclonal-beta-iii-tubulin-antibody-310202415533839.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Increased lactate level detected in the hippocampus of streptozotocin-injected mice fed an HFD (HFD + STZ).b LDHA and LDHB protein levels were measured by western blot assay. c LDHA protein levels were quantified by the ratio to B-actin in the hippocampus. f Cropped images of LDHA and LDHB in the cortex. g Quantified LDHA in the cortex by the ratio to B-actin. Values are expressed as means +/- SEMs (n = 5 for both groups). * p < 0.05. Image collected and cropped by Citeab from the following publication (Hyperpolarized [1-13C]lactate flux increased in the hippocampal region in diabetic mice. Mol Brain (2019) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Western-Blot-NBP1-48336-img0020.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Increased lactate level detected in the hippocampus of streptozotocin-injected mice fed an HFD (HFD + STZ).b LDHA and LDHB protein levels were measured by western blot assay. c LDHA protein levels were quantified by the ratio to B-actin in the hippocampus. f Cropped images of LDHA and LDHB in the cortex. g Quantified LDHA in the cortex by the ratio to B-actin. Values are expressed as means +/- SEMs (n = 5 for both groups). * p < 0.05. Image collected and cropped by Citeab from the following publication (Hyperpolarized [1-13C]lactate flux increased in the hippocampal region in diabetic mice. Mol Brain (2019) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Immunocytochemical analysis of Lactate Dehydrogenase A in HeLa cells](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-48336-img0009.jpg)
![Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Staining of postnatal day 8(P8) Plp-EGFP transgenic mice brain section. Image from verified customer review.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Immunohistochemistry-NBP1-48336-img0013.jpg)
![Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - An intracellular stain was performed on HeLa cells with Lactate Dehydrogenase A/LDHA Antibody NBP1-48336AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Flow-Cytometry-NBP1-48336-img0021.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Analysis of Lactate Dehydrogenase A in HeLa whole cell lysates.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Western-Blot-NBP1-48336-img0011.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Analysis of Lactate Dehydrogenase A in NIH/3T3 whole cell lysates.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Western-Blot-NBP1-48336-img0012.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Lactate Dehydrogenase A expression in bovine skeletal muscle lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Western-Blot-NBP1-48336-img0014.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Lactate Dehydrogenase A expression in porcine skeletal muscle lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Western-Blot-NBP1-48336-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - The effect of the co-culturing of cancer cells and fibroblasts on the expression of Hk2 and Ldha. In the co-cultures, the expression of Ldha was reduced in cancer cells, but was elevated in fibroblasts. Bar = 50 um. The ratio of the fluorescence of Hk2 and Ldha to beta-actin in the MLF monoculture was assumed to be 1. All the experiments were performed in triplicate and obtained similar results, and representative data from one experiment are shown in the figure. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31947613/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lactate Dehydrogenase A-LDHA Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NBP1-48336-img0022.jpg)
![Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Staining of Lactate Dehydrogenase A in lung tissue.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Immunohistochemistry-NBP1-48336-img0010.jpg)
![Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336]](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Flow-Cytometry-NBP1-48336-img0017.jpg)
![Simple Western: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Lane view shows a specific band for Lactate dehydrogenase A in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Simple-Western-NBP1-48336-img0016.jpg)
![Simple Western: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Protein changes in ONHAs corroborate bioenergetics data. (A) Glucose transporter-1 protein levels in Stretched ONH astrocytes are significantly higher than Control (*p = 0.0225, n = 7 Control, n = 8 Stretch). Retinal lysate from a 2 month-old mouse was used as a positive control for each protein analyzed, while negative control was the signal obtained when no primary antibody was included in the capillary. (B) Lactate dehydrogenase-A, the astrocyte-specific isoform of the enzyme that catalyzes the interconversion of pyruvate & lactate, has equivalent protein levels in Control & Stretch ONH astrocytes. (C) Glucose-6-phosphate dehydrogenase, the enzyme that shunts glucose into the pentose phosphate pathway, is no different in Control & Stretch ONH astrocytes. (D) Glutamine synthetase, the enzyme that synthesizes glutamine from glutamate, is no different in Control & Stretch ONH astrocytes. (E) The monomeric form of glutamate-aspartate transporter (GLAST) has significantly higher protein levels in the Stretch as compared to the Control ONH astrocytes (p = 0.020; n = 4 Control, n = 5 Stretch). (F) GLAST dimer protein levels are no different in Control & Stretch ONH astrocytes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35992925), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-48336_rabbit-polyclonal-lactate-dehydrogenase-a-ldha-antibody-310202416171412.jpg)
![Immunohistochemistry Free-Floating: GFAP Antibody [NBP1-05198] - Staining of a section of mouse hippocampus stained with chicken pAb to GFAP, NBP1-05198, dilution 1:5000 in green and costained with rabbit pAb to FOX3/NeuN, dilution 1:5000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, mouse brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with the above antibodies. The GFAP antibody stains a network of astroglial cells while the Fox3/NeuN antibody stains the nuclei and proximal perikarya of neurons.](http://images.novusbio.com/fullsize/GFAP-Antibody-NBP1-05198-img0001.jpg "Immunohistochemistry Free-Floating: GFAP Antibody [NBP1-05198] - Staining of a section of mouse hippocampus stained with chicken pAb to GFAP, NBP1-05198, dilution 1:5000 in green and costained with rabbit pAb to FOX3/NeuN, dilution 1:5000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, mouse brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with the above antibodies. The GFAP antibody stains a network of astroglial cells while the Fox3/NeuN antibody stains the nuclei and proximal perikarya of neurons.")
![Western Blot: GFAP Antibody [NBP1-05198] - Analysis of whole brain lysates using chicken pAb to GFAP, NBP1-05198, dilution 1:5000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain. The strong band at about 50 kDa corresponds to the GFAP protein. Smaller proteolytic fragments and alternate transcripts of GFAP may also be detected on such blots.](http://images.novusbio.com/fullsize/GFAP-Antibody-NBP1-05198-img0002.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Immunohistochemistry-Paraffin-NB110-60531-img0011.jpg "Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.")
![Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Western-Blot-NB110-60531-img0013.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0014.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - 50 ug protein per lane for liver lysate and 0.5 ul of plasma at 9% SDS. Samples are loaded onto the gel as the following order: human liver lysate, mouse liver lysate, rat liver lysate, human plasma, and mouse plasma. Image from confirmed customer review.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Western-Blot-NB110-60531-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ApoE (WUE-4) [NB110-60531] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Immunocytochemistry-Immunofluorescence-NB110-60531-img0010.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - Analysis of apoB-positivity in blood plasma and PLT concentrate-derived EXOs. FCM detection of the indicated markers in EXOs conjugated onto latex beads. The EXOs were isolated from fasting PFP or PLT concentrate by differential UC and gravity size filtration (gray histograms: blocked beads incubated with antibody, empty histograms: EXO sample). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep24316), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0020.jpg)
![Flow (Intracellular): Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with NB110-60531AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Intracellular-NB110-60531-img0012.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with NB110-60531PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0015.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with Apolipoprotein E/ApoE Antibody NB110-60531 [WUE-4] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0016.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with alpha-Synuclein [2A7] Antibody NB110-60531AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0017.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE Antibody [WUE-4] NB110-60531F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0018.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE [WUE-4] Antibody NB110-60531PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0019.jpg)
![Western Blot: MBP Antibody (12) [NB600-717] - Mouse Brain Tissue lysate probed with Rat anti MBP.](http://images.novusbio.com/fullsize/MBP-Antibody-12-Western-Blot-NB600-717-img0002.jpg "Western Blot: MBP Antibody (12) [NB600-717] - Mouse Brain Tissue lysate probed with Rat anti MBP.")
![Immunocytochemistry/Immunofluorescence: MBP Antibody (12) [NB600-717] - Histological and mRNA analysis of the inflammatory cytokines in the vehicle- or etifoxine-treated mice at onset of clinical symptoms. At day 10 p.i., drug treated animals showed significant differences in MBP staining. Animals treated with etifoxine showed an increase in retention of percentage of MBP coverage (*p = 0.001). Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/cgi/doi/10.1002/emmm.201202124), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MBP-Antibody-12-Immunocytochemistry-Immunofluorescence-NB600-717-img0005.jpg)
![Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse brain corpus callosum. Image from verified customer review.](http://images.novusbio.com/fullsize/MBP-Antibody-12-Immunohistochemistry-Frozen-NB600-717-img0004.jpg)
![Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse spinal cord ventral white matter. Image from verified customer review.](http://images.novusbio.com/fullsize/MBP-Antibody-12-Immunohistochemistry-Frozen-NB600-717-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: MBP Antibody (12) [NB600-717] - Microglia processes appear to preferentially contact TBI-induced proximal axonal swellings. Representative 3D reconstructions of MBP+ myelinated axons (red) or APP+ axonal swellings (green) & Iba-1+ microglia (white) in sham-injured (a) or central fluid percussion injured (b) thalami. c Bar graph depicting the average number of Iba-1+ microglial processes contacting either MBP+ myelinated fibers in the sham animals or APP+ axonal swellings in injured animals. Graph depicts the mean ± standard error of the mean. *p < 0.05. Scale bar: 5 μm Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-015-0405-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-717_rat-monoclonal-mbp-antibody-12-31020241535646.jpg)
![Simple Western: GAPDH Antibody [NB300-322] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-322-img0005.jpg "Simple Western: GAPDH Antibody [NB300-322] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.")
![Western Blot: GAPDH Antibody [NB300-322] - Detection of Human and Mouse GAPDH (theoretical molecular weight: 36 kDa) by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-322 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0007.jpg)
![Western Blot: GAPDH Antibody [NB300-322] - Detection of Human and Mouse GAPDH (theoretical molecular weight: 36 kDa) by Western Blot. Samples: Whole cell lysate from mouse NIH3T3 (5, 15 and 50 ug) and human HeLa (H; 50 ug) cells. Antibody: Affinity purified rabbit anti-GAPDH antibody used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0006.jpg)
![Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-322-img0008.jpg)
![Western Blot: GAPDH Antibody [NB300-322] - c-Src is required for efficient translation of PRC2 component mRNAs. Immunoblot of PRC2 components in control and c-Src-deficient tumors. Bar chart shows quantification of fluorescent immunoblot data normalized to the loading control (Gapdh). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10681-4), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-322] - GAPDH detection in HeLa cells with ICC-IF application using NB300-322, visualized with DyLight Fluor 488.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NB300-322-img0003.jpg)
![Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-322-img0009.jpg)
![Western Blot: GAPDH Antibody [NB300-322] - Mouse DRG stained at 1:2000 dilution. Image provided by verified customer review.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0004.jpg)
![Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase chemokinesis and chemotaxis. Western blot analyses were performed on 70 ug of cellular extracts from MOLT4 cells transduced by Lenti-control or Lenti-GEM viral particles. Membranes were probed with anti-Gem (1:2,000) or anti-GAPDH (1:1,000) antibody. ***: significantly different, p 0.1), Fgenotype(1,30) = 30.7 (p Int J Mol Sci (2024). Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-12220251513483.jpg)
![Simple Western: GAPDH Antibody [NB300-322] - Astroglial expression in the plasma membrane evoked by ripple burst and fast ripple burst stimulations in wild type and S286L-TG. Panel (A) indicates expression of pannexin1 in the astroglial plasma membrane fraction. Ordinates indicate the mean ± SD (n = 6) of relative expression of pannexin1 per GAPDH. Gray and green bars indicate pannexin1 expression in astrocytes of wild type and S286L-TG after chronic fast ripple-evoked stimulation, respectively. * p 0.1), Fgenotype*age(1,20) = 5.4 (p Int J Mol Sci (2024). Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-12220251513482.jpg)
![Western Blot: Parvalbumin Antibody [NB120-11427] - WB analysis of rat cerebellum extract.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NB120-11427-img0002.jpg "Western Blot: Parvalbumin Antibody [NB120-11427] - WB analysis of rat cerebellum extract.")
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in U251 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0008.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Stargazin expression is restricted to PV+ interneurons in WT mouse somatosensory cortex (upper row). Somatodendritic stargazin immunoreactivity is lost in PV+ interneurons in stargazer mouse (lower row). Co-labeling of stargazin and parvalbumin antibodies shown in layer 2/3 at 63x magnification (scale = 10 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2013.00156/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in C6 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in Hela Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0007.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human cerebellum tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0003.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human skeletal muscle tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0004.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human tonsil tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody - BSA Free [NB120-11427] - Stargazin expression is restricted to PV+ interneurons in WT mouse somatosensory cortex (upper row). Somatodendritic stargazin immunoreactivity is lost in PV+ interneurons in stargazer mouse (lower row). Co-labeling of stargazin & parvalbumin antibodies shown in layer 2/3 at 63× magnification (scale = 10 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24065886), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-11427_rabbit-polyclonal-parvalbumin-antibody-310202415284515.jpg)
![Immunohistochemistry: Parvalbumin Antibody - BSA Free [NB120-11427] - Female Ambra1+/− mice show reduced numbers of hippocampal PV interneurons. a Nissl staining of dorsal hippocampus coronal sections from WT & Ambra1+/− female mice (scale bar: 500 μm). b Subdivision of hippocampal layers (stratum oriens, CA1 pyramidal layer, stratum radiatum, stratum lacunosum moleculare, stratum moleculare, dentate gyrus upper blade, hilus, dentate gyrus lower blade), corresponding to the area depicted in a (scale bar: 100 μm). Ambra1+/− females show normal hippocampal layering (n = 3 mice per genotype). c Double labeling for Ambra1 (green) & PV (red) in the CA1 from a WT female; sections were DAPI-counterstained (blue) for neuronal nuclei (scale bar: 50 μm). The higher magnification image (scale bar, 5 μm) from the stratum pyramidale shows a PV neuron (right; merge in orange) & an unidentified neuron (left), both expressing Ambra1. The DAPI signal is omitted for clarity & neuron borders are indicated by a dash line (n = 3 mice; three sections per animal). d, e Immunohistochemical labeling of PV in a coronal brain section of dorsal hippocampus obtained from female (d) & male (e) mice (scale bars: 500 μm). On the right are higher magnification images (scale bars: 50 μm). The plots show stereological quantification of PV interneurons (n = 6 mice per genotype & per gender; 14 sections per animal). The reduction in PV interneuron numbers is selective for female Ambra1+/− (two-tailed unpaired t test: *P = 0.043) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29488136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-11427_rabbit-polyclonal-parvalbumin-antibody-310202416205149.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - The effects of a high calcium/phosphate diet, of 1,25(OH)2D3, and of antioxidant supplementation on oxidative stress, DNA damage, and protein expression of oncogenes and tumor suppressive genes in 1alpha(OH)ase -/- mice. Mice from each group were treated as described in Figure 1. Representative micrographs of skin sections stained immunohistochemically for SOD2 and gamma-H2AX. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/acel.12951), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-NB100-1992-img0013.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - The effects of a high calcium/phosphate diet, of 1,25(OH)2D3, and of antioxidant supplementation on oxidative stress, DNA damage, and protein expression of oncogenes and tumor suppressive genes in 1alpha(OH)ase -/- mice. Mice from each group were treated as described in Figure 1. Representative micrographs of skin sections stained immunohistochemically for SOD2 and gamma-H2AX. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/acel.12951), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody at 1:120 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 120 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Localization: Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1992-img0010.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - Western blot analysis of Rat Tissue lysates showing detection of SOD2/Mn-SOD protein using Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Western-Blot-NB100-1992-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody at 1:120 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 120 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Localization: Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1992-img0012.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - Immunohistochemistry analysis using Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Mitochondrion matrix.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-NB100-1992-img0017.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-31020241555245.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-3102024168392.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-31020241553748.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - CAV-1 preserves mitochondrial respiratory function through regulation of OXPHOS expression.(A) OXPHOS complex subunits were detected through western blotting with appropriate antibodies. CAV-1 treatment significantly restored hypercholesterolemia-associated increased ETC complex I–V proteins levels. (B) Percentage of the OXPHOS complex (I–V) band intensities is presented in the graph. (C) Representative immunoblot displaying levels of CAV-1, CyPA, mitochondrial biogenesis markers, & antioxidant enzymes in the NC, HC, & HCAV-1 groups. (D) Columns represent average values over three independent experiments. The density for the NC group was set at 1; * & † are significantly different for the NC & HC groups, respectively, at P < 0.05. beta -actin was used as a loading control. Values are means ± SD. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0154210), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-3102024155523.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-310202415541978.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-310202415535131.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-3102024165716.jpg)
![Western Blot: RelA/NFkB p65 Antibody [NB100-2176] - Human glomerular endothelial cell lysates, time-response treatment with 10ng/ml TNF-alpha. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-Western-Blot-NB100-2176-img0006.jpg "Western Blot: RelA/NFkB p65 Antibody [NB100-2176] - Human glomerular endothelial cell lysates, time-response treatment with 10ng/ml TNF-alpha. This image was submitted via customer Review.")
![Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody [NB100-2176] - Staining of formalin-fixed, paraffin-embedded human DLBCL showing nuclear expression of NFkB p65 in the tumor cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-Immunohistochemistry-Paraffin-NB100-2176-img0004.jpg)
![Western Blot: RelA/NFkB p65 Antibody [NB100-2176] - Analysis of NFkB p65 expression in A431 (A) and HeLa (B) whole cell lysates.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-Western-Blot-NB100-2176-img0005.jpg)
![Immunohistochemistry-Paraffin: Rabbit Polyclonal RelA/NFkB p65 Antibody [NB100-2176] - RelA/NFkB p65 IHC (brown) in the mice liver tissue; bars, 200 μm. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-2176_rabbit-polyclonal-rela-nfkb-p65-antibody-immunohistochemistry-paraffin-1111202410307..jpg)
![Immunocytochemistry/Immunofluorescence: Rabbit Polyclonal RelA/NFkB p65 Antibody [NB100-2176] - Mice hepatocytes stained for RelA/NFkB p65. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-2176_rabbit-polyclonal-rela-nfkb-p65-antibody-immunocytochemistry-immunofluorescence-2511202410544..png)