GADD153/CHOP Antibody (9C8) [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
9C8
Clonality
Monoclonal
Host
Mouse

GADD153/CHOP Antibody (9C8) [DyLight 650] Summary

Immunogen
Full length mouse CHOP/GADD153 [Swiss-Prot# P35639]
Epitope
The N-terminal region of CHOP/GADD153.
Localization
Nuclear
Marker
ER Stress Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DDIT3
Purity
Protein A purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 650
Format
DyLight 650 (A=652, E=672)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This CHOP/GADD153 Antibody (9C8) is useful for Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band can be seen at ~29 kDa. Use in Rat reported in customer review. Gel Super Shift Assays was reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat and primate.

Alternate Names for GADD153/CHOP Antibody (9C8) [DyLight 650]

  • C/EBP-homologous protein 10
  • C/EBP-homologous protein
  • CCAAT/enhancer-binding protein homologous protein
  • CEBP zeta
  • CHOP
  • CHOP10 CEBPZ
  • CHOP10
  • CHOP-10
  • CHOPC/EBP zeta
  • DDIT3
  • DDIT-3
  • DNA damage-inducible transcript 3 protein
  • DNA-damage-inducible transcript 3
  • GADD153
  • Growth arrest and DNA damage-inducible protein GADD153

Background

GADD153, also known as CHOP or DDIT3, is as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein functions as a transcriptional inhibitor by forming heterodimers with C/EBP (CCAAT/enhancer-binding protein) and LAP (liver activator protein), and preventing their DNA binding activity. Under normal growth conditions GADD153 is dormant; however it is highly unregulated during cellular stress caused by toxins, metabolic inhibitors and nutrient deprivation. GADD153 is present in the cytosol under nonstressed conditions and then accumulates in the nucleus once it is induced by stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GADD153/CHOP Antibody (NB600-1335C) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB600-1335APC
Biotin Labeled NB600-1335B
DyLight 350 Labeled NB600-1335UV
DyLight 405 Labeled NB600-1335V
DyLight 405LS Labeled NB600-1335V3
DyLight 488 Labeled NB600-1335G
DyLight 550 Labeled NB600-1335R
DyLight 680 Labeled NB600-1335FR
DyLight 755 Labeled NB600-1335IR
FITC Labeled NB600-1335F
HRP Labeled NB600-1335H
PE Labeled NB600-1335PE
PerCP Labeled NB600-1335PCP

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Research Areas for GADD153/CHOP Antibody (NB600-1335C)

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Blogs on GADD153/CHOP

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol DDIT3
Entrez

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