LRRK2 Antibody [Biotin]

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Product Details

Summary
Reactivity Hu, Mu, Bv, CeSpecies Glossary
Applications WB, ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

LRRK2 Antibody [Biotin] Summary

Immunogen
A C-terminal synthetic peptide made to the human LRRK2 protein sequence (between residues 2500-2527). [UniProt# Q5S007]
Localization
Mitochondrial outer membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LRRK2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
In Western blot. a band can be seen at ~286 kDa. We have also seen other bands with some lysates, but these bands have been blocked by the control peptide, suggesting that these bands are degradation products. IP has been done in an LRRK2 autophosphorylation kinase assay, IHC has been done on brain sections and ICC/IF has been done on transfected cell lines. Frozen sections using the LRRK2 antibody were from a customer review.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
286 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NB300-268B in the following applications:

Reactivity Notes

Drosophila and moth. Immunogen sequence has 87% homology to rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for LRRK2 Antibody [Biotin]

  • augmented in rheumatoid arthritis 17
  • AURA17
  • Dardarin
  • DKFZp434H2111
  • EC 2.7.11.1
  • FLJ45829
  • leucine-rich repeat kinase 2
  • leucine-rich repeat serine/threonine-protein kinase 2
  • LRRK2
  • PARK8
  • PARK8DARDARIN
  • Parkinson disease (autosomal dominant) 8
  • RIPK7
  • ROCO2

Background

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LRRK2 Antibody (NB300-268B) (0)

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Review for LRRK2 Antibody (NB300-268B) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ELISA.

Reviews using NB300-268B:
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ELISA
(1)
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Images Ratings Applications Species Date Details
ELISA LRRK2 NB300-268B
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reviewed by:
Anonymous
ELISA Human 10/24/2017
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Summary

ApplicationELISA
Sample TestedHuman PBMC Whole Cell Lysate
SpeciesHuman
LotG-3-100217-B

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LRRK2 Antibody (NB300-268B) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 488 Labeled NB300-268G
DyLight 550 Labeled NB300-268R
DyLight 650 Labeled NB300-268C
HRP Labeled NB300-268H

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Bioinformatics Tool for LRRK2 Antibody (NB300-268B)

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Research Areas for LRRK2 Antibody (NB300-268B)

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Blogs on LRRK2.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1,...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Parkinson's Disease Infographic
Parkinson's disease affects the nervous system which controls movement. Damage to the levels of dopamine in the brain impairs the ability to relay messages to parts of the body which control movement. While the exact cause of the disease is unknown, r...  Read full blog post.

Novus Antibodies Highlighted in Parkinson's Disease Research
Identified almost two centuries ago, Parkinson's disease is a neurodegenerative disorder that afflicts an estimated 4-6 million worldwide (www.parkinsons.org). The prevalence of Parkinson's disease is expected to grow considerably as the average age o...  Read full blog post.

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Recent Reviews

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1
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Anonymous
10/24/2017
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol LRRK2
Entrez