Synapsin I Antibody

Western Blot: Synapsin I Antibody [NB300-104] - 10 ug of rat brain lysate showing specific immunolabeling of the ~78 kDa Synapsin I doublet.

Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] - ICC-IF validation analysis of Synapsin 1 Antibody on cultured rat caudate neurons. This representative image shows the punctate distribution of Synapsin 1 (green) and MAP (red) proteins in a neuronal cell.

Immunohistochemistry-Paraffin: Synapsin I Antibody [NB300-104] - Human cerebellum section, formalin fixed paraffin embedded. Stained with Synpasin-1 at 1:250. Citrate antigen retrieval at pH 6. Image from verified customer review.

Western Blot: Synapsin I Antibody [NB300-104] - 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~78k synapsin I doublet protein.

• Reactivity: Hu, Mu, Rt, Gp, Pm
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Synapsin I Antibody Summary

• Immunogen: Native protein purified from bovine brain
• Localization: Synapse, Synaptic vesicle, Golgi apparatus
• Marker: pre-Synaptic Marker
• Specificity: Specific for the ~78k synapsin I doublet in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1 ug per 200ug lysate
  • Immunohistochemistry Free-Floating
• Application Notes: NB 300-104 can be used in Western blot where a doublet is seen at ~ 80 kDa representing synapsin Ia and Ib. Immunofluoresence, Immunoprecipitation (1 ug will quantitatively immunoprecipitate all synapsin in 200 ug of rat brain.) May work in IHC-Paraffin but this has not been tested. Differential distribution of the synapsins in the rat olfactory bulb. Has been reported in the literature to work in WB (rat cortex), IHC (cryosections) on rat olfacotry bulb. (PMID: 8283238) Use in Immunohistochemistry free floating reported in multiple pieces of scientific literature.
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Synapsin I Overexpression Lysate
  Synapsin I Knockout A549 Cell Lysate

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 20384782). Guinea Pig reactivity reported in scientific literature (PMID: 25339870).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Immunogen affinity purified

Notes

PLEASE NOTE: If 0.01mg size is ordered, product will come lyophilized from 5 mM ammonium bicarbonate. Please reconstitute lyophilized product in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4)

Alternate Names for Synapsin I Antibody

• SYN1
• SYN1a
• SYN1b
• Synapsin 1
• Synapsin I
• synapsin IBrain protein 4.1
• synapsin-1
• synaptic marker
• synaptic markers immunohistochemistry
• SYNI

Background

Synapsin I, also called Synapsin-1 or SYN1, belongs to Synapsin family (Synapsin I, Synapsin II, Synapsin III) of evolutionarily conserved phospho-proteins which are associated with the cytosolic side of the synaptic vesicles and tether the latter to actin cytoskeleton, thus forming a reserve pool. Synapsins represents the most abundant of neuron-specific phosphor-proteins, consisting 9% of the total amount of all vesicle proteins in neurons wherein they play a critical role in the regulation of neurotransmitter release. Synapsin I is present in axon terminals and plays a role in regulating the secretion of neurotransmitters; thus, it influences synaptic plasticity by regulating pre- and post-synaptic vesicular release. Synapsins I and II are typically found in mature synapses, while synapsin III is often attributed to developing synapses with lesser overall expression. Phosphorylation at Ser-9 residue results in dissociation of Synapsins I from synaptic vesicles which is important for synaptic vesicle neurotransmitter release. Mutations in Synapsin I have been linked to Epilepsy X-linked, with variable learning disabilities and behavior disorders (XELBD) which is a neurologic disorder characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Synapsin I Antibody (NB300-104)(42)

Publications using NB300-104

• Lin L, Petralia RS, Lake R et al. A novel structure associated with aging is augmented in the DPP6-KO mouse brain Acta Neuropathol Commun Nov 23 2020 [PMID: 33225987] (IHC, Mouse)
  
  Details:
  DPP6-KO and wild type mice hippocampi were analyzed via immunohistochemistry
• Petrova N, Nutma E, Carassiti D et al. Synaptic Loss in Multiple Sclerosis Spinal Cord Ann. Neurol. Jun 30 2020 [PMID: 32608018] (IHC, Human)
• Maslon, MM;Braunschweig, U;Aitken, S;Mann, AR;Kilanowski, F;Hunter, CJ;Blencowe, BJ;Kornblihtt, AR;Adams, IR;Caceres, JF; A slow transcription rate causes embryonic lethality and perturbs kinetic coupling of neuronal genes EMBO J. May 2 2019 [PMID: 30988016] (ICC/IF, Mouse)
• Lai HM, Liu AKL, Ng HHM et al. Next generation histology methods for three-dimensional imaging of fresh and archival human brain tissues. Nat Commun. Mar 14 2018 [PMID: 29540691] (IHC, Human)
• Lam CS, Tipoe GL, Wong JK et al. M30 Antagonizes Indoleamine 2,3-Dioxygenase Activation and Neurodegeneration Induced by Corticosterone in the Hippocampus. PLoS ONE. Nov 21 2016 [PMID: 27870896] (WB, Rat)
• Scofield MD, Li H, Siemsen BM et al. Cocaine Self-Administration and Extinction Leads to Reduced Glial Fibrillary Acidic Protein Expression and Morphometric Features of Astrocytes in the Nucleus Accumbens Core. Biol Psychiatry. 2015 Dec 31 [PMID: 26946381] (IHC-Fr, Rat)
• Liu C, Song X, Nisbet R, Gotz J. Co-immunoprecipitation with tau isoform-specific antibodies reveals distinct protein interactions, and highlights a putative role for 2N tau in disease. J Biol Chem. 2016 Feb 9 [PMID: 26861879] (IP, Mouse)
• Gho CG, Schomann T, de Groot SC et al. Isolation, expansion and neural differentiation of stem cells from human plucked hair: a further step towards autologous nerve recovery. Cytotechnology. 2015 Dec 24 [PMID: 26702932] (WB, Human)
• Tang LT, Craig TJ, Henley JM. SUMOylation of synapsin Ia maintains synaptic vesicle availability and is reduced in an autism mutation. Nat Commun. 2015 Jul 15 [PMID: 26173895] (WB, ICC/IF, Mouse, Rat)
• Graser S, Mentrup B, Schneider D et al. Overexpression of tissue-nonspecific alkaline phosphatase increases the expression of neurogenic differentiation markers in the human SH-SY5Y neuroblastoma cell line. Bone. 2015 Oct 1 [PMID: 26032516] (ICC/IF, Human)
• Koga K, Liu M G et al. Impaired presynaptic long-term potentiation in the anterior cingulate cortex of Fmr1 knock-out mice. J Neurosci Apr 2 2015 [PMID: 25653361] (WB, Mouse)
• Mellott JG, Bickford ME, Schofield BR. Descending projections from auditory cortex to excitatory and inhibitory cells in the nucleus of the brachium of the inferior colliculus. Front Syst Neurosci. 2014 Oct 6 [PMID: 25339870] (WB, IHC-FrFl, Guinea Pig)
• Ho VM, Dallalzadeh LO, Karathanasis N et al. GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons. Mol Cell Neurosci. 2014 Jul 1 [PMID: 24784359] (ICC/IF, Mouse)
• Hu Q, Liang X, Chen D et al. Delayed hyperbaric oxygen therapy promotes neurogenesis through reactive oxygen species/hypoxia-inducible factor-1alpha/beta-catenin pathway in middle cerebral artery occlusion rats. Stroke. 2014 Jun 1 [PMID: 24757104] (IHC-Fr, Mouse)
• Paemka L, Mahajan VB, Skeie JM et al. PRICKLE1 interaction with SYNAPSIN I reveals a role in autism spectrum disorders. PLoS One. 2013 Dec 3 [PMID: 24312498] (WB, IP, ICC/IF, Mouse)
• Federici M, Latagliata EC, Ledonne A et al. Paradoxical abatement of striatal dopaminergic transmission by cocaine and methylphenidate. J. Biol. Chem. 2014 Jan 3 [PMID: 24280216] (WB, Mouse)
• Efthymiou A, Shaltouki A, Steiner JP et al. Functional screening assays with neurons generated from pluripotent stem cell-derived neural stem cells. J Biomol Screen. 2014 Jan 1 [PMID: 24019252] (ICC/IF, Human)
• Mori F, Nistico R, Mandolesi G et al. Interleukin-1beta Promotes Long-Term Potentiation in Patients with Multiple Sclerosis. Neuromolecular Med 2013 Jul 28 [PMID: 23892937] (WB, Mouse)
• Puglisi F, Vanni V, Ponterio G et al. Torsin A Localization in the Mouse Cerebellar Synaptic Circuitry. PLoS One 2013 Jun 19 [PMID: 23840813] (WB, Mouse)
• Cesca F, Limongi T, Accardo A. Fabrication of biocompatible free-standing nanopatterned films for primary neuronal cultures. RSC Adv. 2014 (ICC/IF, Mouse)
• Paonessa F, Latifi S, Scarongella H et al. Specificity protein 1 (Sp1)-dependent activation of the synapsin I gene (SYN1) is modulated by RE1-silencing transcription factor (REST) and 5'-cytosine-phosphoguanine (CpG) methylation. J Biol Chem. 2013 Feb 1 [PMID: 23250796] (WB, ICC/IF, Human, Mouse)
• Bueno C, Martinez-Morga M, Martinez S. Nuclear polymorphism and non-proliferative adult neurogenesis in human neural crest-derived cells. bioRxiv May 18 2018
• Limongi T, Cesca F, Gentile F et al. Nanostructured superhydrophobic substrates trigger the development of 3D neuronal networks. Small. 2013 Feb 11 [PMID: 23027505] (ICC/IF, Mouse)
• Ramos C, Chardonnet S, Marchand CH et al. Native presynaptic metabotropic glutamate receptor 4 (mGluR4) interacts with exocytosis proteins in rat cerebellum. J Biol Chem. 2012 Jun 8 [PMID: 22528491] (WB, Rat)
• Trushina E, Nemutlu E, Zhang S et al. Defects in mitochondrial dynamics and metabolomic signatures of evolving energetic stress in mouse models of familial Alzheimer's disease. PLoS One. 2012 [PMID: 22393443] (ICC/IF, Mouse)
• Cronin T, Lyubarsky A, Bennett J. Dark-rearing the rd10 mouse: implications for therapy. Adv Exp Med Biol. 2012 [PMID: 22183325] (IHC-Fr, Mouse)
• Ren WW, Liu Y, Li BM. Stimulation of alpha(2A)-adrenoceptors promotes the maturation of dendritic spines in cultured neurons of the medial prefrontal cortex. Mol Cell Neurosci. 2012 Feb 1 [PMID: 22015717] (WB, ICC/IF, Mouse)
• Lasagna-Reeves CA, Castillo-Carranza DL, Sengupta U et al. Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice. Mol Neurodegener. 2011 Jun 6 [PMID: 21645391] (WB, Mouse)
• VanGuilder HD, Farley JA, Yan H et al. Hippocampal dysregulation of synaptic plasticity-associated proteins with age-related cognitive decline. Neurobiol Dis. 2011 Jul 1 [PMID: 21440628] (WB, Rat)
• Steinman MQ, Crean KK, Trainor BC. Photoperiod interacts with food restriction in performance in the Barnes maze in female California mice. Eur J Neurosci. 2011 Jan [PMID: 21198981] (WB, IHC-FrFl, Mouse)
• Hsieh CY, Nakamura PA, Luk SO et al. Ephrin-B reverse signaling is required for formation of strictly contralateral auditory brainstem pathways. J Neurosci. 2010 Jul 21 [PMID: 20660266] (IHC-FrFl, Mouse)
• Sherwood CC, Duka T, Stimpson CD et al. Neocortical synaptophysin asymmetry and behavioral lateralization in chimpanzees (Pan troglodytes). Eur J Neurosci. 2010 Apr 1 [PMID: 20384782] (WB, Primate)
• Gelman BB, Nguyen TP Synaptic proteins linked to HIV-1 infection and immunoproteasome induction: proteomic analysis of human synaptosomes. J Neuroimmune Pharmacol. 2010 Mar 1 [PMID: 19693676] (WB, Human)
• Kim SS, Yoo SW, Park TS et al. Neural induction with neurogenin1 increases the therapeutic effects of mesenchymal stem cells in the ischemic brain. Stem Cells. 2008 Sep 1 [PMID: 18617687] (IHC-P, Rat)
• Kennedy, N et al. Requirements of JIP scaffold proteins for NMDA-mediated signal transduction. Genes Development; 21: 2336-2346. 2007 [PMID: 17875667] (ICC/IF, Mouse)
• Thalhammer A, Rudhard Y, Tigaret CM et al. CaMKII translocation requires local NMDA receptor-mediated Ca2+ signaling. EMBO J. 2006 Dec 13 [PMID: 17124502] (ICC/IF, Mouse)
• Hanus, C, Ehrensperger, M-V, Triller, A. Activity-Dependent Movements of Postsynaptic Scaffolds at Inhibitory Synapses. 26, 4586-4595. 2006 [PMID: 16641238] (ICC/IF, Rat)
• Rastaldi MP, Armelloni S, Berra S et al. Glomerular podocytes contain neuron-like functional synaptic vesicles. FASEB J. 2006 May 1 [PMID: 16585060] (IHC-Fr, Mouse)
• Specht CG, Tigaret CM, Rast GF et al. Subcellular localisation of recombinant alpha- and gamma-synuclein. Mol Cell Neurosci. 2005 Feb [PMID: 15691713] (ICC/IF, Mouse)
• Danglot, L et al. Association of gephyrin with synaptic extrasynaptic GABAA receptors varies during development in cultured hippocampal neurons. Molecular Cellular Neuroscience; 23:264-278. 2003 [PMID: 12812758]
• Stone LM, Browning MD, Finger TE. Differential distribution of the synapsins in the rat olfactory bulb. J Neurosci. 1994 Jan [PMID: 8283238] (WB, IHC-Fr, Rat)
  
  Details:
  WB (rat cortex), Fig 2; IHC (cryosections) on rat olfacotry bulb, Figs 5 and 6.
• Bueno C, Martinez-Morga M, Martinez S. Non-proliferative adult neurogenesis in neural crest-derived stem cells isolated from human periodontal ligament. bioRxiv May 18 2018 (ICC/IF, Human)

Review for Synapsin I Antibody (NB300-104) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous IHC-P Human 04/17/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Brain (cerebellum) tissue
• Species: Human
• Lot: ajo516g

Comments

• Comments: Citrate antigen retrieval, pH6
  1 in 250 dilution

FAQs for Synapsin I Antibody (NB300-104). (Showing 1 - 1 of 1 FAQs).

1. How many days do you recommend culturing primary neurons to get a good Synapsin I signal? Can you please send the protocol used to validate this antibody in immunofluorescence?
   • It was validated in IF by a collaborator, so I do not have a specific protocol to offer. However I can tell you that the neurons were cultured for 14 days.

Bioinformatics

• Gene Symbol: SYN1
• Entrez:
  • 6853(Human)
  • 20964(Mouse)
  • 24949(Rat)
• Uniprot:
  • P17600(Human)