Synapsin I Antibody - Azide Free

Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] - hPDLSCs-derived neural-like cells are connected by synapse-like interactions. hPDLSCs-derived neural-like cells connect to one another. Synapse-associated protein Synapsin1 is found in the cell membrane of hPDLSCs-derived neural-like cells at the neurite contact areas. Scale bar: 25 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-54745-3) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] - Characterization of WT and slow ESCs differentiated to neurons. Immunofluorescence staining for neuronal markers Map2, and Syn1 in neurons cultured on poly-ornithine-/laminin-coated plates. Expression of neuronal markers in neurons cultured on poly-ornithine-/laminin-coated plates from RNA-seq analysis, n = 3. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/embj.2018101244) licensed under a CC-BY license.

Western Blot: Synapsin I Antibody [NB300-104] - Western blot analysis showing induced expression of neuronal functional marker Synapsin I at protein levels in MNC (meningial neuronal construct). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30443545/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Synapsin I Antibody [NB300-104] - Human cerebellum section, formalin fixed paraffin embedded. Stained with Synpasin-1 at 1:250. Citrate antigen retrieval at pH 6. Image from verified customer review.

Western Blot: Synapsin I Antibody [NB300-104] - 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~78k synapsin I doublet protein.

Western Blot: Synapsin I Antibody [NB300-104] - 10 ug of rat brain lysate showing specific immunolabeling of the ~78 kDa Synapsin I doublet.

Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] - ICC-IF validation analysis of Synapsin 1 Antibody on cultured rat caudate neurons. This representative image shows the punctate distribution of Synapsin 1 (green) and MAP (red) proteins in a neuronal cell.

Characterization of WT and slow ESCs differentiated to neurons (related to Fig 4)Immunofluorescence staining for neuronal markers Map2, Syn1, and NeuN in neurons cultured on poly‐ornithine‐/laminin‐coated plates.Expression of neuronal markers in neurons cultured on poly‐ornithine‐/laminin‐coated plates from RNA‐seq analysis, n = 3.

Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104] - Characterization of WT & slow ESCs differentiated to neurons (related to Fig 4)Immunofluorescence staining for neuronal markers Map2, Syn1, & NeuN in neurons cultured on poly‐ornithine‐/laminin‐coated plates.Expression of neuronal markers in neurons cultured on poly‐ornithine‐/laminin‐coated plates from RNA‐seq analysis, n = 3. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30988016), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104] - hPDLSCs-derived neural-like cells are connected by synapse-like interactions. hPDLSCs-derived neural-like cells connect to one another (a) through different types of synapses-like interactions, including dendrodendritic-like, axoaxonic-like & axodendritic-like synapses (b). (c) Synapse-associated proteins Cx43, Synaptophysin & Synapsin1 are found in the cell membrane of hPDLSCs-derived neural-like cells at the neurite contact areas. Scale bar: 25 μm. AA, axoaxonic-like synapse; AD, axodendritic-like synapse; DD, dendrodendritic-like synapse; LM, light microscopy; SEM, scanning electron microscopy. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31792338), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Synapsin I Antibody [NB300-104] - Synaptic localization of TA at the PC–PF synaptic contacts.Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein (PSD95) & Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) & TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C–E) The high magnification of the inset in B is reproduced in different merge images & by the relative split channels. C) Merge image of Cb & V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D) Merge image of TA & Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E) Merge image of TA & V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) & TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23840813), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Synapsin I Antibody [NB300-104] - Tau oligomers induce synaptic dysfunction. (A) Representative Western blot of mouse hippocampus homogenate. The levels of synaptophysin, synapsin-1, & septin-11 were measured by band quantification & normalized with the levels of tubulin. PBSo indicates representative bands of hippocampal area injected with PBS in mice also injected with tau oligomers, PBSf indicates PBS injection in mice also injected with tau fibrils, & PBSm indicates PBS injection in mice also injected with tau monomers. (B) Synaptophysin levels were significantly lower in the hemispheres injected with tau oligomers in comparison with the ones injected with fibrils, monomers, or PBS. (C) No significant differences in the levels of synapsin-1 were observed. (D) Only the hemisphere injected with tau oligomers presents a decrease in the level of septin-11. Data are represented as the mean ± SE. *p < 0.01, n = 6 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21645391), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Gp, Pm
• Applications: WB, ICC/IF, IHC, IP, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Synapsin I Antibody - Azide Free Summary

• Description: PLEASE NOTE: If 0.01mg size is ordered, product will come lyophilized from 5 mM ammonium bicarbonate. Please reconstitute lyophilized product in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4)
• Immunogen: Native protein purified from bovine brain. Accession # P17599
• Localization: Synapse, Synaptic vesicle, Golgi apparatus
• Marker: pre-Synaptic Marker
• Specificity: Specific for endogenous levels of the ~78 kDa synapsin I doublet. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1 ug per 200ug lysate
  • Western Blot 1:1000
• Application Notes: NB 300-104 can be used in Western blot where a doublet is seen at ~ 80 kDa representing synapsin Ia and Ib. Immunofluoresence, Immunoprecipitation (1 ug will quantitatively immunoprecipitate all synapsin in 200 ug of rat brain.) May work in IHC-Paraffin but this has not been tested. Differential distribution of the synapsins in the rat olfactory bulb. Has been reported in the literature to work in WB (rat cortex), IHC (cryosections) on rat olfacotry bulb. (PMID: 8283238) Use in Immunohistochemistry free floating reported in multiple pieces of scientific literature .
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Synapsin I Overexpression Lysate

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 20384782). Guinea Pig reactivity reported in scientific literature (PMID: 25339870).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Synapsin I Antibody - Azide Free

• SYN1
• SYN1a
• SYN1b
• Synapsin 1
• Synapsin I
• synapsin IBrain protein 4.1
• synapsin-1
• synaptic marker
• synaptic markers immunohistochemistry
• SYNI

Background

Synapsin I, also called Synapsin-1 or SYN1, belongs to Synapsin family (Synapsin I, Synapsin II, Synapsin III) of evolutionarily conserved phospho-proteins which are associated with the cytosolic side of the synaptic vesicles and tether the latter to actin cytoskeleton, thus forming a reserve pool. Synapsins represents the most abundant of neuron-specific phosphor-proteins, consisting 9% of the total amount of all vesicle proteins in neurons wherein they play a critical role in the regulation of neurotransmitter release. Synapsin I is present in axon terminals and plays a role in regulating the secretion of neurotransmitters; thus, it influences synaptic plasticity by regulating pre- and post-synaptic vesicular release. Synapsins I and II are typically found in mature synapses, while synapsin III is often attributed to developing synapses with lesser overall expression. Phosphorylation at Ser-9 residue results in dissociation of Synapsins I from synaptic vesicles which is important for synaptic vesicle neurotransmitter release. Mutations in Synapsin I have been linked to Epilepsy X-linked, with variable learning disabilities and behavior disorders (XELBD) which is a neurologic disorder characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Synapsin I Antibody (NB300-104)(53)

Publications using NB300-104

• Karliner J, Merry DE Differentiating PC12 cells to evaluate neurite densities through live-cell imaging STAR protocols 2023-01-04 [PMID: 36602900] (WB, ICC/IF, Rat)
  
  Details:
  Dilution used in WB and ICC/IF 1:1000
• Hei Ming Lai, Yumi Tang, Zachary Y H Lau, Robert A A Campbell, Juno C N Yau, Caleb C Y Chan, Danny C W Chan, Tin Yan Wong, Harriet K T Wong, Leo Y C Yan, William K K Wu, Sunny H Wong, Ka-Wai Kwok, Yun-Kwok Wing, Henry H N Lam, Ho-Keung Ng, Thomas D Mrsic-Flogel, Vincent C T Mok, Jason Y K Chan, Ho Ko Antibody stabilization for thermally accelerated deep immunostaining. Nature methods 2022-09-14 [PMID: 36050489]
• Štepánková K, Chudí?ková M, Šimková Z et al. Oral administration of 4-methylumbelliferone reduces glial scar and promotes anatomical plasticity bioRxiv 2023-02-04 (IHC-FrFl, Rat)
• Štepánková K, Chudí?ková M, Šimková Z et al. Low oral dose of 4-methylumbelliferone reduces glial scar but is insufficient to induce functional recovery after spinal cord injury Scientific reports 2023-11-06 [PMID: 37932336] (IHC, Rat)
• Tang C, Liu M, Zhou Z et al. Treadmill Exercise Alleviates Cognition Disorder by Activating the FNDC5: Dual Role of Integrin ?V/?5 in Parkinson’s Disease International Journal of Molecular Sciences 2023-04-25 [PMID: 37175535] (Western Blot, Mouse)
• Ma KG, Hu HB, Zhou JS Et al. Neuronal Glypican4 promotes mossy fiber sprouting through the mTOR pathway after pilocarpine-induced status epilepticus in mice Experimental neurology 2021-11-05 [PMID: 34748756] (WB, IF/IHC, Mouse)
• Rizzo FR, Guadalupi L, Sanna K Et al. Exercise protects from hippocampal inflammation and neurodegeneration in experimental autoimmune encephalomyelitis Brain, behavior, and immunity 2021-08-12 [PMID: 34391817] (WB, Mouse)
• Yang Y, Zhang J, Yang X et al. Dysregulated APP expression and alpha-secretase processing of APP is involved in manganese-induced cognitive impairment Ecotoxicology and environmental safety 2021-05-28 [PMID: 34058678] (ICC/IF, WB, Mouse)
• Lamotte J, Roqueviere S, Gautier H, et al. hiPSC-Derived Neurons Provide a Robust and Physiologically Relevant In Vitro Platform to Test Botulinum Neurotoxins Frontiers in Pharmacology 2021-01-14 [PMID: 33519485] (ICC/IF, Human)
• Lin L, Petralia RS, Lake R et al. A novel structure associated with aging is augmented in the DPP6-KO mouse brain Acta Neuropathol Commun 2020-11-23 [PMID: 33225987] (IF/IHC, Mouse)
  
  Details:
  DPP6-KO and wild type mice hippocampi were analyzed via immunohistochemistry
• Petrova N, Nutma E, Carassiti D et al. Synaptic Loss in Multiple Sclerosis Spinal Cord Ann. Neurol. 2020-06-30 [PMID: 32608018] (IF/IHC, Human)
• Bueno C, Martinez-Morga M, Martinez S. Nuclear polymorphism and non-proliferative adult neurogenesis in human neural crest-derived cells. bioRxiv 2018-05-18
• Bueno C, Martinez-Morga M, Martinez S. Non-proliferative adult neurogenesis in neural crest-derived stem cells isolated from human periodontal ligament. bioRxiv 2018-05-18 [PMID: 31792338] (ICC/IF, Human)
• Bueno C, Martinez-Morga M, et al. Non-proliferative neurogenesis in human periodontal ligament stem cells. Sci Rep 2019-12-02 [PMID: 31792338] (ICC/IF, Human)
• Maslon, MM;Braunschweig, U;Aitken, S;Mann, AR;Kilanowski, F;Hunter, CJ;Blencowe, BJ;Kornblihtt, AR;Adams, IR;Caceres, JF; A slow transcription rate causes embryonic lethality and perturbs kinetic coupling of neuronal genes EMBO J. 2019-05-02 [PMID: 30988016] (ICC/IF, Mouse)
• Vishwakarma S. K, Bardia A, et al. Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury. Front Bioeng Biotechnol 2018-11-18 [PMID: 30443545] (WB, IF/IHC, Human)
• Lai HM, Liu AKL, Ng HHM et al. Next generation histology methods for three-dimensional imaging of fresh and archival human brain tissues. Nat Commun. 2018-03-14 [PMID: 29540691] (IF/IHC, Human)
• Lam CS, Tipoe GL, Wong JK et al. M30 Antagonizes Indoleamine 2,3-Dioxygenase Activation and Neurodegeneration Induced by Corticosterone in the Hippocampus. PLoS ONE. 2016-11-21 [PMID: 27870896] (WB, Rat)
• Cesca F, Limongi T, Accardo A. Fabrication of biocompatible free-standing nanopatterned films for primary neuronal cultures. RSC Adv. 2014-01-01 (ICC/IF, Mouse)
• Scofield MD, Li H, Siemsen BM et al. Cocaine Self-Administration and Extinction Leads to Reduced Glial Fibrillary Acidic Protein Expression and Morphometric Features of Astrocytes in the Nucleus Accumbens Core. Biol Psychiatry. 2015-12-31 [PMID: 26946381] (IHC-Fr, Rat)
• Liu C, Song X, Nisbet R, Gotz J. Co-immunoprecipitation with tau isoform-specific antibodies reveals distinct protein interactions, and highlights a putative role for 2N tau in disease. J Biol Chem. 2016-02-09 [PMID: 26861879] (IP, Mouse)
• Gho CG, Schomann T, de Groot SC et al. Isolation, expansion and neural differentiation of stem cells from human plucked hair: a further step towards autologous nerve recovery. Cytotechnology. 2015-12-24 [PMID: 26702932] (WB, Human)
• Tang LT, Craig TJ, Henley JM. SUMOylation of synapsin Ia maintains synaptic vesicle availability and is reduced in an autism mutation. Nat Commun. 2015-07-15 [PMID: 26173895] (WB, ICC/IF, Mouse, Rat)
• Graser S, Mentrup B, Schneider D et al. Overexpression of tissue-nonspecific alkaline phosphatase increases the expression of neurogenic differentiation markers in the human SH-SY5Y neuroblastoma cell line. Bone. 2015-10-01 [PMID: 26032516] (ICC/IF, Human)
• Koga K, Liu M G et al. Impaired presynaptic long-term potentiation in the anterior cingulate cortex of Fmr1 knock-out mice. J Neurosci 2015-04-02 [PMID: 25653361] (WB, Mouse)
• Mellott JG, Bickford ME, Schofield BR. Descending projections from auditory cortex to excitatory and inhibitory cells in the nucleus of the brachium of the inferior colliculus. Front Syst Neurosci. 2014-10-06 [PMID: 25339870] (WB, IHC-FrFl, Guinea Pig)
• Ho VM, Dallalzadeh LO, Karathanasis N et al. GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons. Mol Cell Neurosci. 2014-07-01 [PMID: 24784359] (ICC/IF, Mouse)
• Hu Q, Liang X, Chen D et al. Delayed hyperbaric oxygen therapy promotes neurogenesis through reactive oxygen species/hypoxia-inducible factor-1alpha/beta-catenin pathway in middle cerebral artery occlusion rats. Stroke. 2014-06-01 [PMID: 24757104] (IHC-Fr, Mouse)
• Paemka L, Mahajan VB, Skeie JM et al. PRICKLE1 interaction with SYNAPSIN I reveals a role in autism spectrum disorders. PLoS One. 2013-12-03 [PMID: 24312498] (ICC/IF, WB, IP, Mouse)
• Federici M, Latagliata EC, Ledonne A et al. Paradoxical abatement of striatal dopaminergic transmission by cocaine and methylphenidate. J. Biol. Chem. 2014-01-03 [PMID: 24280216] (WB, Mouse)
• Efthymiou A, Shaltouki A, Steiner JP et al. Functional screening assays with neurons generated from pluripotent stem cell-derived neural stem cells. J Biomol Screen. 2014-01-01 [PMID: 24019252] (ICC/IF, Human)
• Mori F, Nistico R, Mandolesi G et al. Interleukin-1beta Promotes Long-Term Potentiation in Patients with Multiple Sclerosis. Neuromolecular Med 2013-07-28 [PMID: 23892937] (WB, Mouse)
• Puglisi F, Vanni V, Ponterio G et al. Torsin A Localization in the Mouse Cerebellar Synaptic Circuitry. PLoS One 2013-06-19 [PMID: 23840813] (WB, Mouse)
• Paonessa F, Latifi S, Scarongella H et al. Specificity protein 1 (Sp1)-dependent activation of the synapsin I gene (SYN1) is modulated by RE1-silencing transcription factor (REST) and 5'-cytosine-phosphoguanine (CpG) methylation. J Biol Chem. 2013-02-01 [PMID: 23250796] (WB, ICC/IF, Mouse, Human)
• Limongi T, Cesca F, Gentile F et al. Nanostructured superhydrophobic substrates trigger the development of 3D neuronal networks. Small. 2013-02-11 [PMID: 23027505] (ICC/IF, Mouse)
• Ramos C, Chardonnet S, Marchand CH et al. Native presynaptic metabotropic glutamate receptor 4 (mGluR4) interacts with exocytosis proteins in rat cerebellum. J Biol Chem. 2012-06-08 [PMID: 22528491] (WB, Rat)
• Trushina E, Nemutlu E, Zhang S et al. Defects in mitochondrial dynamics and metabolomic signatures of evolving energetic stress in mouse models of familial Alzheimer's disease. PLoS One. 2012-01-01 [PMID: 22393443] (ICC/IF, Mouse)
• Cronin T, Lyubarsky A, Bennett J. Dark-rearing the rd10 mouse: implications for therapy. Adv Exp Med Biol. 2012-01-01 [PMID: 22183325] (IHC-Fr, Mouse)
• Ren WW, Liu Y, Li BM. Stimulation of alpha(2A)-adrenoceptors promotes the maturation of dendritic spines in cultured neurons of the medial prefrontal cortex. Mol Cell Neurosci. 2012-02-01 [PMID: 22015717] (ICC/IF, WB, Mouse)
• Lasagna-Reeves CA, Castillo-Carranza DL, Sengupta U et al. Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice. Mol Neurodegener. 2011-06-06 [PMID: 21645391] (WB, Mouse)
• VanGuilder HD, Farley JA, Yan H et al. Hippocampal dysregulation of synaptic plasticity-associated proteins with age-related cognitive decline. Neurobiol Dis. 2011-07-01 [PMID: 21440628] (WB, Rat)
• Steinman MQ, Crean KK, Trainor BC. Photoperiod interacts with food restriction in performance in the Barnes maze in female California mice. Eur J Neurosci. 2011-01-01 [PMID: 21198981] (IHC-FrFl, WB, Mouse)
• Hsieh CY, Nakamura PA, Luk SO et al. Ephrin-B reverse signaling is required for formation of strictly contralateral auditory brainstem pathways. J Neurosci. 2010-07-21 [PMID: 20660266] (IHC-FrFl, Mouse)
• Sherwood CC, Duka T, Stimpson CD et al. Neocortical synaptophysin asymmetry and behavioral lateralization in chimpanzees (Pan troglodytes). Eur J Neurosci. 2010-04-01 [PMID: 20384782] (WB, Primate)
• Gelman BB, Nguyen TP Synaptic proteins linked to HIV-1 infection and immunoproteasome induction: proteomic analysis of human synaptosomes. J Neuroimmune Pharmacol. 2010-03-01 [PMID: 19693676] (WB, Human)
• Kim SS, Yoo SW, Park TS et al. Neural induction with neurogenin1 increases the therapeutic effects of mesenchymal stem cells in the ischemic brain. Stem Cells. 2008-09-01 [PMID: 18617687] (IHC-P, Rat)
• Kennedy, N et al. Requirements of JIP scaffold proteins for NMDA-mediated signal transduction. Genes Development; 21: 2336-2346. 2007-01-01 [PMID: 17875667] (ICC/IF, Mouse)
• Thalhammer A, Rudhard Y, Tigaret CM et al. CaMKII translocation requires local NMDA receptor-mediated Ca2+ signaling. EMBO J. 2006-12-13 [PMID: 17124502] (ICC/IF, Mouse)
• Hanus, C, Ehrensperger, M-V, Triller, A. Activity-Dependent Movements of Postsynaptic Scaffolds at Inhibitory Synapses. 26, 4586-4595. 2006-01-01 [PMID: 16641238] (ICC/IF, Rat)
• Rastaldi MP, Armelloni S, Berra S et al. Glomerular podocytes contain neuron-like functional synaptic vesicles. FASEB J. 2006-05-01 [PMID: 16585060] (IHC-Fr, Mouse)
• Specht CG, Tigaret CM, Rast GF et al. Subcellular localisation of recombinant alpha- and gamma-synuclein. Mol Cell Neurosci. 2005-02-01 [PMID: 15691713] (ICC/IF, Mouse)
• Danglot, L et al. Association of gephyrin with synaptic extrasynaptic GABAA receptors varies during development in cultured hippocampal neurons. Molecular Cellular Neuroscience; 23:264-278. 2003-01-01 [PMID: 12812758]
• Stone LM, Browning MD, Finger TE. Differential distribution of the synapsins in the rat olfactory bulb. J Neurosci. 1994-01-01 [PMID: 8283238] (IHC-Fr, WB, Rat)
  
  Details:
  WB (rat cortex), Fig 2; IHC (cryosections) on rat olfacotry bulb, Figs 5 and 6.

Review for Synapsin I Antibody (NB300-104) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 04/17/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Brain (cerebellum) tissue
• Species: Human
• Lot: ajo516g

Comments

• Comments: Citrate antigen retrieval, pH6
  1 in 250 dilution

FAQs for Synapsin I Antibody (NB300-104). (Showing 1 - 1 of 1 FAQs).

1. How many days do you recommend culturing primary neurons to get a good Synapsin I signal? Can you please send the protocol used to validate this antibody in immunofluorescence?
   • It was validated in IF by a collaborator, so I do not have a specific protocol to offer. However I can tell you that the neurons were cultured for 14 days.

Bioinformatics

• Gene Symbol: SYN1
• Entrez:
  • 6853(Human)
  • 20964(Mouse)
  • 24949(Rat)
• Uniprot:
  • P17600(Human)