Reactivity | Hu, Mu, Rt, Gp, PmSpecies Glossary |
Applications | WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl |
Clonality | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Concentration | 0.1 mg/ml |
Immunogen | Native protein purified from bovine brain |
Localization | Synapse, Synaptic vesicle, Golgi apparatus |
Marker | pre-Synaptic Marker |
Specificity | Specific for the ~78k synapsin I doublet in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | SYN1 |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | NB 300-104 can be used in Western blot where a doublet is seen at ~ 80 kDa representing synapsin Ia and Ib. Immunofluoresence, Immunoprecipitation (1 ug will quantitatively immunoprecipitate all synapsin in 200 ug of rat brain.) May work in IHC-Paraffin but this has not been tested. Differential distribution of the synapsins in the rat olfactory bulb. Has been reported in the literature to work in WB (rat cortex), IHC (cryosections) on rat olfacotry bulb. (PMID: 8283238) Use in Immunohistochemistry free floating reported in multiple pieces of scientific literature. |
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Theoretical MW | 78 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Storage | Store at -20C. Avoid freeze-thaw cycles. |
Buffer | 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol |
Preservative | No Preservative |
Concentration | 0.1 mg/ml |
Purity | Immunogen affinity purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Anonymous |
IHC-P | Human | 04/17/2018 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
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Synapsin I, a pre-synaptic marker Synapsin-I, also called Synapsin 1/Syn1, is an ~80 kDa protein (predicted mol. wt. 74.1 kDa) which belongs to the Synapsin family (Synapsin I, Synapsin II, Synapsin III). Synapsins are the evolutionarily conserved phospho-proteins which are associ... Read full blog post. |
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