Synapsin I Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104] - hPDLSCs-derived neural-like cells are connected by synapse-like interactions. hPDLSCs-derived neural-like cells connect to one another. ...read more
Orthogonal Strategies: Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104] - Characterization of WT and slow ESCs differentiated to neurons. Immunofluorescence staining for neuronal markers ...read more
Western Blot: Synapsin I Antibody [NB300-104] - Western blot analysis showing induced expression of neuronal functional marker Synapsin I at protein levels in MNC (meningial neuronal construct). Image collected and ...read more
Immunohistochemistry-Paraffin: Synapsin I Antibody [NB300-104] - Human cerebellum section, formalin fixed paraffin embedded. Stained with Synpasin-1 at 1:250. Citrate antigen retrieval at pH 6. Image from verified ...read more
Western Blot: Synapsin I Antibody [NB300-104] - 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~78k synapsin I doublet protein.
Western Blot: Synapsin I Antibody [NB300-104] - 10 ug of rat brain lysate showing specific immunolabeling of the ~78 kDa Synapsin I doublet.
Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104] - ICC-IF validation analysis of Synapsin 1 Antibody on cultured rat caudate neurons. This representative image shows the punctate distribution of ...read more
Characterization of WT and slow ESCs differentiated to neurons (related to Fig 4)Immunofluorescence staining for neuronal markers Map2, Syn1, and NeuN in neurons cultured on poly‐ornithine‐/laminin‐coated ...read more
Synaptic localization of TA at the PC-PF synaptic contacts.Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic ...read more
Tau oligomers induce synaptic dysfunction. (A) Representative Western blot of mouse hippocampus homogenate. The levels of synaptophysin, synapsin-1, and septin-11 were measured by band quantification and normalized with ...read more
hPDLSCs-derived neural-like cells are connected by synapse-like interactions. hPDLSCs-derived neural-like cells connect to one another (a) through different types of synapses-like interactions, including ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Gp, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IP, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Synapsin I Antibody Summary

Description
PLEASE NOTE: If 0.01mg size is ordered, product will come lyophilized from 5 mM ammonium bicarbonate. Please reconstitute lyophilized product in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4)
Immunogen
Native protein purified from bovine brain. Accession # P17599
Localization
Synapse, Synaptic vesicle, Golgi apparatus
Marker
pre-Synaptic Marker
Specificity
Specific for endogenous levels of the ~78 kDa synapsin I doublet. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYN1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1 ug per 200ug lysate
  • Western Blot 1:1000
Application Notes
NB 300-104 can be used in Western blot where a doublet is seen at ~ 80 kDa representing synapsin Ia and Ib. Immunofluoresence, Immunoprecipitation (1 ug will quantitatively immunoprecipitate all synapsin in 200 ug of rat brain.) May work in IHC-Paraffin but this has not been tested. Differential distribution of the synapsins in the rat olfactory bulb. Has been reported in the literature to work in WB (rat cortex), IHC (cryosections) on rat olfacotry bulb. (PMID: 8283238) Use in Immunohistochemistry free floating reported in multiple pieces of scientific literature .
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Synapsin I Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB300-104 in the following applications:

Publications
Read Publications using
NB300-104 in the following applications:

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 20384782). Guinea Pig reactivity reported in scientific literature (PMID: 25339870).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Antigen Affinity-purified

Alternate Names for Synapsin I Antibody

  • SYN1
  • SYN1a
  • SYN1b
  • Synapsin 1
  • Synapsin I
  • synapsin IBrain protein 4.1
  • synapsin-1
  • synaptic marker
  • synaptic markers immunohistochemistry
  • SYNI

Background

Synapsin I, also called Synapsin-1 or SYN1, belongs to Synapsin family (Synapsin I, Synapsin II, Synapsin III) of evolutionarily conserved phospho-proteins which are associated with the cytosolic side of the synaptic vesicles and tether the latter to actin cytoskeleton, thus forming a reserve pool. Synapsins represents the most abundant of neuron-specific phosphor-proteins, consisting 9% of the total amount of all vesicle proteins in neurons wherein they play a critical role in the regulation of neurotransmitter release. Synapsin I is present in axon terminals and plays a role in regulating the secretion of neurotransmitters; thus, it influences synaptic plasticity by regulating pre- and post-synaptic vesicular release. Synapsins I and II are typically found in mature synapses, while synapsin III is often attributed to developing synapses with lesser overall expression. Phosphorylation at Ser-9 residue results in dissociation of Synapsins I from synaptic vesicles which is important for synaptic vesicle neurotransmitter release. Mutations in Synapsin I have been linked to Epilepsy X-linked, with variable learning disabilities and behavior disorders (XELBD) which is a neurologic disorder characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Synapsin I Antibody (NB300-104)(51)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Guinea Pig, Primate.

We have publications tested in 7 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-FrFl, IHC-P, IP, WB.


Filter By Application
ICC/IF
(20)
IF/IHC
(5)
IHC-Fr
(5)
IHC-FrFl
(4)
IHC-P
(1)
IP
(2)
WB
(24)
All Applications
Filter By Species
Human
(11)
Mouse
(28)
Rat
(10)
Guinea Pig
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 51. Show All 51 Publications.
Publications using NB300-104 Applications Species
Karliner J, Merry DE Differentiating PC12 cells to evaluate neurite densities through live-cell imaging STAR protocols 2023-01-04 [PMID: 36602900] (WB, ICC/IF, Rat)

Details:
Dilution used in WB and ICC/IF 1:1000
WB, ICC/IF Rat
Bueno C, Martinez-Morga M, Martinez S. Nuclear polymorphism and non-proliferative adult neurogenesis in human neural crest-derived cells. bioRxiv 2018-05-18
Cesca F, Limongi T, Accardo A. Fabrication of biocompatible free-standing nanopatterned films for primary neuronal cultures. RSC Adv. 2014-01-01 (ICC/IF, Mouse) ICC/IF Mouse
Štepánková K, Chudí?ková M, Šimková Z et al. Oral administration of 4-methylumbelliferone reduces glial scar and promotes anatomical plasticity bioRxiv 2023-02-04 (IHC-FrFl, Rat) IHC-FrFl Rat
Tang C, Liu M, Zhou Z et al. Treadmill Exercise Alleviates Cognition Disorder by Activating the FNDC5: Dual Role of Integrin ?V/?5 in Parkinson’s Disease International Journal of Molecular Sciences 2023-04-25 [PMID: 37175535] (WB, Mouse) WB Mouse
Ma KG, Hu HB, Zhou JS Et al. Neuronal Glypican4 promotes mossy fiber sprouting through the mTOR pathway after pilocarpine-induced status epilepticus in mice Experimental neurology 2021-11-05 [PMID: 34748756] (WB, IF/IHC, Mouse) WB, IF/IHC Mouse
Rizzo FR, Guadalupi L, Sanna K Et al. Exercise protects from hippocampal inflammation and neurodegeneration in experimental autoimmune encephalomyelitis Brain, behavior, and immunity 2021-08-12 [PMID: 34391817] (WB, Mouse) WB Mouse
Yang Y, Zhang J, Yang X et al. Dysregulated APP expression and alpha-secretase processing of APP is involved in manganese-induced cognitive impairment Ecotoxicology and environmental safety 2021-05-28 [PMID: 34058678] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Lamotte J, Roqueviere S, Gautier H, et al. hiPSC-Derived Neurons Provide a Robust and Physiologically Relevant In Vitro Platform to Test Botulinum Neurotoxins Frontiers in Pharmacology 2021-01-14 [PMID: 33519485] (ICC/IF, Human) ICC/IF Human
Lin L, Petralia RS, Lake R et al. A novel structure associated with aging is augmented in the DPP6-KO mouse brain Acta Neuropathol Commun 2020-11-23 [PMID: 33225987] (IF/IHC, Mouse)

Details:
DPP6-KO and wild type mice hippocampi were analyzed via immunohistochemistry
IF/IHC Mouse
Show All 51 Publications.

Review for Synapsin I Antibody (NB300-104) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB300-104:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Synapsin I NB300-104
Enlarge
5
reviewed by:
Verified Customer
IHC-P Human 04/17/2018
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBrain (cerebellum) tissue
SpeciesHuman
Lotajo516g

Comments

CommentsCitrate antigen retrieval, pH6
1 in 250 dilution

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Synapsin I Antibody (NB300-104). (Showing 1 - 1 of 1 FAQs).

  1. How many days do you recommend culturing primary neurons to get a good Synapsin I signal? Can you please send the protocol used to validate this antibody in immunofluorescence?
    • It was validated in IF by a collaborator, so I do not have a specific protocol to offer. However I can tell you that the neurons were cultured for 14 days.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Synapsin I.

Synapsin I, a pre-synaptic marker
Synapsin-I, also called Synapsin 1/Syn1, is an ~80 kDa protein (predicted mol. wt. 74.1 kDa) which belongs to the Synapsin family (Synapsin I, Synapsin II, Synapsin III). Synapsins are the evolutionarily conserved phospho-proteins which are associ...  Read full blog post.

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Recent Reviews

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Verified Customer
04/17/2018
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol SYN1
Entrez
Uniprot