GADD153/CHOP Antibody (9C8) - Azide and BSA Free

Western Blot: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745] - GADD153/CHOP expression in HeLa cells treated with 2.5 ug/mL tunicamycin for 4 hours (Lane 1) and untreated (Lane 2). Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear

Western Blot: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745] - Analysis of CHOP in rat heart tissue lysate. Image courtesy of product review submitted by Lee Hsiao-Wei. Image from the standard format of this antibody.

Western Blot: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745] - Analysis of endogenous CHOP/GADD153 from primary human fibroblasts using NB600-1335. Lane 1: Untreated cells, Lane 2: Cells treated with tunicamycin for 10 hours. Image from the standard format of this antibody.

Western Blot: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745] - Ethanol feeding increases CHOP expression. Image from verified customer review. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear

Simple Western: GADD153/CHOP Antibody (9C8) - Azide and BSA Free [NBP2-80745] - Image shows a specific band for CHOP/GADD153 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Simple Western, ChIP, ELISA, GS, ICC/IF, IHC, IP, ChIP, Mycoplasma
• Clone: 9C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

GADD153/CHOP Antibody (9C8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse GADD153/CHOP Antibody (9C8) - Azide and BSA Free (NB600-1335) is a monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF, Simple Western, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length mouse CHOP/GADD153 [Swiss-Prot# P35639]
• Epitope: The N-terminal region of CHOP/GADD153.
• Localization: Nuclear
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDIT3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 30962207)
  • ELISA reported in scientific literature (PMID 29915575)
  • Gel Super Shift Assays reported in scientific literature
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Simple Western 1:250
  • Western Blot
• Application Notes: In Western blot a band can be seen at approx. 29 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:250. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GADD153/CHOP Antibody (9C8) - Azide and BSA Free

• C/EBP-homologous protein 10
• C/EBP-homologous protein
• CCAAT/enhancer-binding protein homologous protein
• CEBP zeta
• CHOP
• CHOP10 CEBPZ
• CHOP10
• CHOP-10
• CHOPC/EBP zeta
• DDIT3
• DDIT-3
• DNA damage-inducible transcript 3 protein
• DNA-damage-inducible transcript 3
• GADD153
• Growth arrest and DNA damage-inducible protein GADD153

Background

GADD153, also known as CHOP or DDIT3, is as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein functions as a transcriptional inhibitor by forming heterodimers with C/EBP (CCAAT/enhancer-binding protein) and LAP (liver activator protein), and preventing their DNA binding activity. Under normal growth conditions GADD153 is dormant; however it is highly unregulated during cellular stress caused by toxins, metabolic inhibitors and nutrient deprivation. GADD153 is present in the cytosol under nonstressed conditions and then accumulates in the nucleus once it is induced by stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDIT3