| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77080SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97728SS | |||
| Azide and BSA Free | NBP2-33253 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56580SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56698SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25274 | |||
| BSA Free | NBP2-27332SS | |||
| Azide and BSA Free | NBP2-25274SS | |||
| Azide and BSA Free | NBP2-80982 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56571SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-24861 | |||
| BSA Free | NB100-56720SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56536SS | |||
| Azide and BSA Free | NBP2-37704 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56581SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56563SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-351SS | |||
| Azide and BSA Free | NBP2-80929 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-24865 | |||
| Azide and BSA Free | NBP2-24865SS | |||
![Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - HMGB1 promoted the activation of canonical NLRP3 inflammasome in retinal IR injury. The intravitreal injection of HMGB1 inhibitor, GA, significantly suppressed mRNA (B) and protein (D) production of NLRP3. Data are shown as mean +/- SD. **P < 0.01 vs sham group. Image collected and cropped by CiteAb from the following publication (//www.jneuroinflammation.com/content/12/1/137) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody---BSA-Free-Western-Blot-NBP1-77080-img0011.jpg "Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - HMGB1 promoted the activation of canonical NLRP3 inflammasome in retinal IR injury. The intravitreal injection of HMGB1 inhibitor, GA, significantly suppressed mRNA (B) and protein (D) production of NLRP3. Data are shown as mean +/- SD. **P < 0.01 vs sham group. Image collected and cropped by CiteAb from the following publication (//www.jneuroinflammation.com/content/12/1/137) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Staining of NALP3 in K562 cells with NALP3 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-77080-img0010.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Analysis of NALP3 in mouse lung tissue using anti-NALP3 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-77080-img0003.jpg)
![Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 ug/mL (A) in the absence and (B) in the presence of blocking peptide.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody---BSA-Free-Western-Blot-NBP1-77080-img0009.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - hADSC-Evs suppressed NLRP3 inflammasome activation in ocular surface of experimental mice under desiccating stress. Representative image of immunofluorescent staining of NLRP3 related genes in corneal and conjunctival epithelia of desiccating mice or treated with hADSC-EVs (*conjunctiva; open triangle cornea). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32884023/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NLRP3-NALP3 Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NBP1-77080-img0012.jpg)
![Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - hADSC-Evs suppressed NLRP3 inflammasome activation in ocular surface of experimental mice under desiccating stress. (A) Representative image of immunofluorescent staining of NLRP3 related genes in corneal & conjunctival epithelia of desiccating mice or treated with hADSC-EVs (*conjunctiva; open triangle cornea). (B,C) qRT-PCR showed the mRNA levels of NLRP3 related genes in conjunctiva & cornea (four corneas/conjunctivas were mixed as a sample). Data was shown as mean ± SEM. *p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32884023), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77080_rabbit-polyclonal-nlrp3-nalp3-antibody-31020241535675.jpg)
![Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - HMGB1 promoted the activation of canonical NLRP3 inflammasome in retinal IR injury. The intravitreal injection of exogenous rHMGB1 significantly promoted the mRNA & protein production of NLRP3 (a, c) & ASC (e, g) & activation of caspase-1 (i). Thus, the intravitreal injection of HMGB1 inhibitor, GA, significantly suppressed the production of NLRP3 (b, d) & ASC (f, h) & activation of caspase-1 (j). Data are shown as mean ± SD. *P < 0.05, **P < 0.01 vs sham group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26224068), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77080_rabbit-polyclonal-nlrp3-nalp3-antibody-310202415345338.jpg)
![Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Co-immunoprecipitation assay: Figure showing interaction of NLRP3, NLRC4 & NAIP with ASC during CFT073 infection in THP-1m. Cytoplasmic extracts of THP-1m infected with or without CFT073, 6 h post stimulation were subjected to IP & WB by various antibodies (ASC, NLRP3, NLRC4, & NAIP) as described in material & methods. Cytoplasmic extract used as input was subjected to check NLRP3, ASC, NLRC4 & NAIP expression as shown in figure. ASC antibody was used to co-immunoprecipitate the complex from cytoplasmic extracts & then immunoblotted to detect NLRP3, NLRC4 & NAIP. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31551961), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77080_rabbit-polyclonal-nlrp3-nalp3-antibody-310202415371971.jpg)
![Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - HMGB1 regulated the activation of NLRP3, rather than the activation of caspase-8, via NF-kappa B pathway. a, b NF-kappa B was activated in ischemic retina at the early stage after reperfusion. c, d Intraocular injection rHMGB1 or GA could promote or suppress the production of phosphor-NF-kappa B p65. e, f Intravitreal injection of NF-kappa B p65 inhibitor, JSH-23, significantly reduced the activation of NLRP3 & decreased the processing of IL-1 beta , g rather than caspase-8. However, intravitreal injection of caspase-8 inhibitor, Z-IETD-fmk, decreased the production of phosphor-NF-kappa B p65, obviously (h). Data are shown as mean ± SD. *P < 0.05, **P < 0.01 vs sham group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26224068), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77080_rabbit-polyclonal-nlrp3-nalp3-antibody-310202415291993.jpg)
![Western Blot: TLR5 Antibody (85B152.5) [NBP1-97728] - Normal human bronchial epithelial total cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/TLR5-Antibody-85B152-5-Western-Blot-NBP1-97728-img0003.jpg "Western Blot: TLR5 Antibody (85B152.5) [NBP1-97728] - Normal human bronchial epithelial total cell lysate. Image from verified customer review.")
![Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the FITC conjugate of NBP1-97728. Staining of TLR5 in 10^6 cells using 0.5 ugs of FITC-conjugated antibody: A) Intracellular flow testing of human Ramos cells and B) cell surface flow testing of mouse RAW cells. The shaded (or black) histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.](http://images.novusbio.com/fullsize/TLR5-Antibody-85B152-5-Flow-Cytometry-NBP1-97728-img0009.jpg)
![Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Intracellular flow analysis of TLR5 in Ramos cells using 1 ug of TLR5 antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control antibody; red represents TLR5 antibody.](http://images.novusbio.com/fullsize/TLR5-Antibody-85B152-5-Flow-Cytometry-NBP1-97728-img0002.jpg)
![Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Intracellular flow analysis of TLR5 in 10^6 human lymphocytes using 0.5 ug of NB200-571. The shaded histogram represents cells without antibody, green represents isotype control (BD), and red represents TLR5 antibody.](http://images.novusbio.com/fullsize/TLR5-Antibody-85B152-5-Flow-Cytometry-NBP1-97728-img0006.jpg)
![Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the PE conjugate of NBP1-97728. Staining of TLR5 in 10^6 RAW cells using 0.5 ugs of PE-conjugated antibody. The black histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.](http://images.novusbio.com/fullsize/TLR5-Antibody-85B152-5-Flow-Cytometry-NBP1-97728-img0007.jpg)
![Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the PE conjugate of NBP1-97728. Staining of TLR5 in 10^6 human lymphocytes using 0.5 ugs of PE-conjugated antibody. The shaded histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.](http://images.novusbio.com/fullsize/TLR5-Antibody-85B152-5-Flow-Cytometry-NBP1-97728-img0008.jpg)
![Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - TLR expression in USSCs. Flow cytometry detection of TLRs in USSCs using TLR5 antibody. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20196781/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-97728_mouse-monoclonal-tlr5-antibody-85b152-5-25520231539519.jpg)
![Western Blot: TLR4 Antibody [NB100-56580] - Immunoblot data examining response of primary macrophages from WT and miR-223 knockout mice (KO) to LPS and combined IFNG/LPS; 50ug of whole cell lysate was loaded per lane and run on a denaturing SDS PAGE gel. Densitometry and statistics are presented in (D). All values are expressed as +/- SEM; * indicates p<0.05 via student t-test. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0165962) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NB100-56580-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: TLR4 Antibody [NB100-56580] - Immunoblot data examining response of primary macrophages from WT and miR-223 knockout mice (KO) to LPS and combined IFNG/LPS; 50ug of whole cell lysate was loaded per lane and run on a denaturing SDS PAGE gel. Densitometry and statistics are presented in (D). All values are expressed as +/- SEM; * indicates p<0.05 via student t-test. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0165962) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TLR4 Antibody [NB100-56580] - Analysis of TLR4 in mouse kidney tissue using TLR4 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-Paraffin-NB100-56580-img0004.jpg)
![Western Blot: TLR4 Antibody [NB100-56580] - Analysis of TLR4. (A) 1 ug/lane partial recombinant mouse TLR4 protein (antibody at 2 ug/mL) and (B) RAW cell lysate and (C) Daudi cell lysate (antibody at 5 ug/mL on tumor cell lysate).](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NB100-56580-img0005.jpg)
![Western Blot: MyD88 Antibody [NB100-56698] - The ability of PTX to induce the IL-1 Beta IL-6 cascade depends on the integrity of its multimeric structure and enzymatic activity.Western blots revealing the presence of mature IL-1 Beta, cleaved Casp1, TLR4 and Myd88 in peritoneal fluid or peritoneal cell lysates from mice killed 6 h after injection of PBS, wild-type PTX or PTXmut. Arrowheads indicate the cleaved forms. Beta-actin was used as loading control. Sample size: 8 per group. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1004150) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NB100-56698-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: MyD88 Antibody [NB100-56698] - The ability of PTX to induce the IL-1 Beta IL-6 cascade depends on the integrity of its multimeric structure and enzymatic activity.Western blots revealing the presence of mature IL-1 Beta, cleaved Casp1, TLR4 and Myd88 in peritoneal fluid or peritoneal cell lysates from mice killed 6 h after injection of PBS, wild-type PTX or PTXmut. Arrowheads indicate the cleaved forms. Beta-actin was used as loading control. Sample size: 8 per group. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1004150) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MyD88 Antibody [NB100-56698] - Raw264.7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-MyD88 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MyD88-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56698-img0008.jpg)
![Immunohistochemistry-Paraffin: MyD88 Antibody [NB100-56698] - Tissue section of human liver using at 1:100 dilution. This antibody generated a very specific cytoplasmic staining in the hepatocytes as well as the Kupffer cells (hepatic macrophages), and the latter showed the most intense staining among all other cell types in the section.](http://images.novusbio.com/fullsize/MyD88-Antibody-Immunohistochemistry-Paraffin-NB100-56698-img0005.jpg)
![Western Blot: MyD88 Antibody [NB100-56698] - Analysis of MyD88 in human spleen cell lysate using 0.5 ug/ml of NB100-56698.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NB100-56698-img0003.jpg)
![Immunohistochemistry-Paraffin: MyD88 Antibody [NB100-56698] - Tissue section of human liver using at 1:100 dilution. This antibody generated a very specific cytoplasmic staining in the hepatocytes as well as the Kupffer cells (hepatic macrophages), and the latter showed the most intense staining among all other cell types in the section.](http://images.novusbio.com/fullsize/MyD88-Antibody-Immunohistochemistry-Paraffin-NB100-56698-img0004.jpg)
![Western Blot: MyD88 Antibody - BSA Free [NB100-56698] - TLR & other LPS-response elements in LPS-stimulated HSCs.(A) Microarray data show time-dependent changes in the indicated transcripts. For clarity control gene expression at 1 & 24h is offset to 0.5h & 24.5h respectively. (B) A representative Western blot (left pane) & densitometric analysis (right panel) of the indicated molecules at 24h following stimulation with 10 ng/ml LPS. (C) qPCR analysis of the indicated molecules with p values showing statistical differences. The values (B,C) shown are form 3 separate determinations from different batches of HSCs. Statistical significance was derived from student’s t-test using Microsoft-excel program. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0082159), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56698_rabbit-polyclonal-myd88-antibody-310202415371913.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - THP-1 cells were stained with TLR7 (4G) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0008.jpg "Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - THP-1 cells were stained with TLR7 (4G) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.")
![Flow (Intracellular): TLR7 Antibody (4G6) [NBP2-27332] - Analysis using the PE conjugate of NBP2-27332. Staining of TLR7 in 10^6 human BDCM cells (red) and 0.5 ug of mouse IgG1 isotype control (green). this antibody was used for this test.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Intracellular-NBP2-27332-img0009.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - Analysis using Azide Free version of NBP2-27332. THP-1 cells were stained with TLR7 (4G) NBP2-25274 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0012.jpg)
![Western Blot: TLR7 Antibody (4G6) - BSA Free [NBP2-27332] - The protein of TLR7 were pull down by miR-138-biotin / miR-mut-138-biotin with Streptavidin agarose beads in BV2 cells. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC7710131/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-BSA-Free-Western-Blot-NBP2-27332-img0017.jpg)
![Immunohistochemistry: TLR7 Antibody (4G6) [NBP2-27332] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of TLR7 [4G6] antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Immunohistochemistry-NBP2-27332-img0016.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - An intracellular stain was performed on Ramos cells with TLR7 Antibody (4G6) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0015.jpg)
![Western Blot: TLR7 Antibody (4G6) [NBP2-27332] - TLR7 antibodies tested at 2 ug/ml on recombinant partial hTLR7 protein (amino acids 562-839). NB100-56682 and NBP2-24767 immunogen sequences are not present in recombinant protein; *NB100-56588 is not recommended for use in western blot.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Western-Blot-NBP2-27332-img0005.jpg)
![Western Blot: TLR7 Antibody (4G6) [NBP2-27332] - Analysis using Azide Free version of NBP2-27332. Human TLR7 antibody in Ramos and transfected 293T lysate using TLR7 monoclonal antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Western-Blot-NBP2-27332-img0011.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - Analysis of TLR7 in human monocytes using 2 ug of TLR7 monoclonal antibody (red) and 2 ug of mouse IgG1 isotype control antibody (green). TLR intracellular flow kit was used for this test, and an anti-mouse IgG PE conjugated secondary antibody .](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0002.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - An intracellular stain was performed on Daudi cells with TLR7 Antibody (4G6) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0013.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - An intracellular stain was performed on Raji cells with TLR7 Antibody (4G6) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0014.jpg)
![Western Blot: TLR7 Antibody (4G6) - BSA Free [NBP2-27332] - ADEV miR‐138 interacts with murine TLR7 in the endosomes. (A) Representative fluorescence images of mouse primary microglial cells incubated with Exo‐Fect+ADEVs+Cy5‐miR138 or Exo‐Fect+Cy5‐miR138 (without ADEVs) or Exo‐Fect+ADEVs+miR138 (unstained) for 30 min followed by immunostaining of (A) an early endosome marker (EEA1, Green) & (B) TLR7 (Green). Exo‐fect+ADEVs+Cy5‐miR138, Cy5‐miR138 were loaded into ADEVs using by Exo‐Fect transfection kit; Exo‐fect+Cy5‐miR138, Cy5‐miR138 were loaded using by Exo‐Fect transfection kit (without ADEVs); Exo‐fect+ADEVs+miR138, unstained miR138 were loaded into ADEVs using Exo‐Fect transfection kit; Bars, 50 μm (n = 3). (C) TLR7 was immunoprecipitated from BV2 cells by IgG / TLR7 / LAMP1 antibody, followed by assessment of miR138 / U6 expression by real‐time PCR. One‐way ANOVA followed by Bonferroni's post hoc test was used to determine the statistical significance among multiple groups (n = 3). (D) TLR7 was immunoprecipitated from HEK‐Null / HEK‐TLR7 cells by IgG/TLR7 antibody, followed by assessment of miR138 expression by real‐time PCR. One‐way ANOVA followed by Bonferroni's post hoc test was used to determine the statistical significance among multiple groups (n = 3). (E) The protein of TLR7 were pull down by miR‐138‐biotin / miR‐mut‐138‐biotin with Streptavidin agarose beads in BV2 cells. All data are presented as mean ± SD or SEM of three individual experiments. *,P < 0.05; **,P < 0.01; ***,P < 0.001 versus control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33304479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-27332_mouse-monoclonal-tlr7-antibody-4g6-310202416114724.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression and its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) and (B) show mRNA and protein levels of TLR3 in sham and MI hearts. n = 4 mice/group. Data are means +/- S.D. AP < 0.01 versus sham. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.13328) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR3-Antibody-Western-Blot-NB100-56571-img0009.jpg "<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression and its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) and (B) show mRNA and protein levels of TLR3 in sham and MI hearts. n = 4 mice/group. Data are means +/- S.D. AP < 0.01 versus sham. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.13328) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Frozen: TLR3 Antibody [NB100-56571] - Frozen mouse eye tissue sections stained with TLR3, a retinal pigment epithelium (RPE) marker and DAPI. Top panel: TLR3 (red) colocalized with the RPE (green) marker. Bottom panel: Tissue stained with isotype control (no TLR3 Ab), RPE and DAPI. Data courtesy of Kleinman et al.](http://images.novusbio.com/fullsize/TLR3-Antibody-Immunohistochemistry-Frozen-NB100-56571-img0002.jpg)
![Flow Cytometry: TLR3 Antibody [NB100-56571] - Analysis using the FITC conjugate of NB100-56571. Staining of TLR3 in Balb/c mouse splenocytes using this antibody at 1 ug/10^6 cells. Green represents rabbit IgG isotype control this antibody; red represents anti-TLR3 antibody.](http://images.novusbio.com/fullsize/TLR3-Antibody-Flow-Cytometry-NB100-56571-img0008.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Analysis of TLR3 in mouse spleen tissue lysate using NB100-56571 at 2 ug/ml.](http://images.novusbio.com/fullsize/TLR3-Antibody-Western-Blot-NB100-56571-img0005.jpg)
![Immunohistochemistry: TLR3 Antibody [NB100-56571] - Pig lung poisoned through aspiration with gastric juice and organophosphate (dimethoate EC40) after 48 hours of IC ventilation. Image from verified customer review.](http://images.novusbio.com/fullsize/TLR3-Antibody-Immunohistochemistry-NB100-56571-img0006.jpg)
![Flow Cytometry: TLR3 Antibody [NB100-56571] - Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes and mouse RAW cells using TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-Flow-Cytometry-NB100-56571-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TLR3 Antibody [NB100-56571] - siRNA was transfected at 50 nM for 48 hrs, and poly(I:C) was added at 100 ug/ml for 4 hrs before cell harvest. Negative control (NC) siRNA served as control. RNAiMAX transfection reagent was used in all the siRNA experiments. Panel shows the knockdown effects of siRNAs. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.13328) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR3-Antibody-Knockdown-Validated-NB100-56571-img0010.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression & its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) & (B) show mRNA & protein levels of TLR3 in sham & MI hearts. n = 4 mice/group. Data are means ± S.D. AP < 0.01 versus sham. (C) Representative immunohistochemistry images of heart sections stained for TLR3 (brown colour). An isotype IgG control was performed to verify the specificity of TLR3 reactivity. (D) Lysates of heart tissue were immunoprecipitated with anti‐TLR3 antibodies (IP: TLR3), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: IgG) was performed as a control to exclude the non‐specific binding of antibodies to cellular proteins. Green arrows indicate non‐specific bands. The association between TLR3 & Trif, but not MyD88, was detectable in sham myocardium & was increased in infarct myocardium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-310202415523938.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression & its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) & (B) show mRNA & protein levels of TLR3 in sham & MI hearts. n = 4 mice/group. Data are means ± S.D. AP < 0.01 versus sham. (C) Representative immunohistochemistry images of heart sections stained for TLR3 (brown colour). An isotype IgG control was performed to verify the specificity of TLR3 reactivity. (D) Lysates of heart tissue were immunoprecipitated with anti‐TLR3 antibodies (IP: TLR3), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: IgG) was performed as a control to exclude the non‐specific binding of antibodies to cellular proteins. Green arrows indicate non‐specific bands. The association between TLR3 & Trif, but not MyD88, was detectable in sham myocardium & was increased in infarct myocardium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-3102024165731.jpg)
![Immunohistochemistry: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression & its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) & (B) show mRNA & protein levels of TLR3 in sham & MI hearts. n = 4 mice/group. Data are means ± S.D. AP < 0.01 versus sham. (C) Representative immunohistochemistry images of heart sections stained for TLR3 (brown colour). An isotype IgG control was performed to verify the specificity of TLR3 reactivity. (D) Lysates of heart tissue were immunoprecipitated with anti‐TLR3 antibodies (IP: TLR3), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: IgG) was performed as a control to exclude the non‐specific binding of antibodies to cellular proteins. Green arrows indicate non‐specific bands. The association between TLR3 & Trif, but not MyD88, was detectable in sham myocardium & was increased in infarct myocardium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-310202415535158.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - TLR3‐knockout (TLR3‐KO) attenuated cardiac autophagy induced by MI. The infarct & remote tissues were separately sampled from the left ventricle after 4 weeks of MI. Accordingly, anterior & posterior tissues of the left ventricle were sampled from sham hearts as controls. (A) Representative Western blot images & quantitative analyses of autophagy markers. n = 4–8/group. Quantitative data are fold changes of WT‐sham. aP < 0.05, AP < 0.01 versus respective WT‐sham; bP < 0.05, BP < 0.01 versus respective WT‐MI. (B) Representative electron microphotographs of ultrathin sections of resin‐embedded heart biopsies. Arrows indicate autophagic vacuoles. (C) Lysates of infarct tissue were immunoprecipitated (IP), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG served as a control. Green arrows indicate non‐specific bands. Representative images were taken from four independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-310202415525214.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - A TLR3 agonist polyinosinic‐polycytidylic acid (poly(I:C)) induced autophagy in cultured cardiomyocytes through a TRIF‐dependent pathway. (A) Poly(I:C) increased autophagy markers in cultured H9c2 rat ventricular cells. (B) Poly(I:C) stimulated autophagosome formation but did not affect autophagic flux. Primary cultured neonatal rat ventricular myocytes (NRVMs) were transfected with a tandem mRFP‐GFP‐LC3 adenovirus for 24 hrs, followed by treatment with poly(I:C) (100 μg/ml, 4 hrs). Autophagosomes & autolysosomes were, respectively, visualized as yellow‐ & red‐only punctas under a confocal microscope. (C) An autophagic flux inhibitor chloroquine (CQ) induced accumulations of LC3‐II & p62/SQSTM1 proteins in H9c2 myocytes receiving poly(I:C) (100 μg/ml, 4 hrs). CQ was applied at 10 μM, immediately prior to poly(I:C). (D) Effects of indicated siRNA on poly(I:C)‐induced changes in autophagy markers in NRVMs. All the siRNAs were transfected at 50 nM for 48 hrs, & poly(I:C) was added at 100 μg/ml for 4 hrs before cell harvest. Negative control (NC) siRNA served as control. RNAiMAX transfection reagent was used in all the siRNA experiments. The upper panel shows the knockdown effects of siRNAs, & the lower panel shows representative Western blot images (presented from four independent experiments) & densitometry quantitative data (normalized into ‘fold of vehicle group’). All quantitative data are expressed as means ± S.D. aP < 0.05, AP < 0.01 versus vehicle; bP < 0.05, BP < 0.01 versus poly(I:C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-3102024168394.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TLR2 Antibody [NB100-56720] - Analysis using the Azide Free version of NB100-56720. Detection of TLR2 in transfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/TLR2-Antibody-Western-Blot-NB100-56720-img0005.jpg)
![Immunohistochemistry-Paraffin: TLR2 Antibody [NB100-56720] - Analysis of mouse pancreas tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/TLR2-Antibody-Immunohistochemistry-Paraffin-NB100-56720-img0003.jpg)
![Immunohistochemistry-Paraffin: TLR6 Antibody (86B1153.2) [NB100-56536] - Tonsil probed with TLR6 antibody at 5 ug/ml. Novus's human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/TLR6-Antibody-86B1153-2-Immunohistochemistry-Paraffin-NB100-56536-img0007.jpg "Immunohistochemistry-Paraffin: TLR6 Antibody (86B1153.2) [NB100-56536] - Tonsil probed with TLR6 antibody at 5 ug/ml. Novus's human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.")
![Flow (Intracellular): TLR6 Antibody (86B1153.2) [NB100-56536] - Analysis using the PE conjugate of NB100-56536. Staining of TLR6 in Ramos cells using this antibody. 5 ug/10^6 cells. Shaded histogram represents Ramos cells without antibody; green represents isotype control (BD Pharmingen); red represents this antibody.](http://images.novusbio.com/fullsize/TLR6-Antibody-86B1153-2-Flow-Intracellular-NB100-56536-img0014.jpg)
![Flow Cytometry: TLR6 Antibody (86B1153.2) [NB100-56536] - Cell surface analysis of TLR6 antibody in 10^6 human monocytes using 0.5 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody ; red represents anti-TLR6 antibody. goat anti-mouse IgG PE conjugated secondary antibody was used.](http://images.novusbio.com/fullsize/TLR6-Antibody-86B1153-2-Flow-Cytometry-NB100-56536-img0006.jpg)
![Western Blot: TLR4 Antibody [NB100-56581] - Analysis of TLR4 using NB100-56581 at 2 ug/mL on partial recombinant mouse TLR4 protein.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NB100-56581-img0008.jpg "Western Blot: TLR4 Antibody [NB100-56581] - Analysis of TLR4 using NB100-56581 at 2 ug/mL on partial recombinant mouse TLR4 protein.")
![Immunocytochemistry/Immunofluorescence: TLR4 Antibody [NB100-56581] - TLR4 antibody was tested in PC-12 cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56581-img0007.jpg)
![Immunohistochemistry-Paraffin: TLR4 Antibody [NB100-56581] - Analysis of TLR4 in FFPE mouse spleen tissue using NB100-56581 (A) and mouse isotype control (B) at 5 mg/mL.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-Paraffin-NB100-56581-img0009.jpg)
![Flow Cytometry: TLR1 Antibody [NB100-56563] - TLR1 expression pattern in primary BMNCs from 3 MM patients analyzed by flow cytometry. CD138-positive cells were gated from the total cell population. Staining for was compared with isotype-matched controls (NC). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR1-Antibody-Flow-Cytometry-NB100-56563-img0016.jpg "Flow Cytometry: TLR1 Antibody [NB100-56563] - TLR1 expression pattern in primary BMNCs from 3 MM patients analyzed by flow cytometry. CD138-positive cells were gated from the total cell population. Staining for was compared with isotype-matched controls (NC). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.")
![Western Blot: TLR1 Antibody [NB100-56563] - analysis of TLR1 in Ramos (A), Raw (B), and (C) TLR1 transfected cell lysate using this antibody. goat anti-rabbit HRP secondary antibody was used.](http://images.novusbio.com/fullsize/TLR1-Antibody-Western-Blot-NB100-56563-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: TLR1 Antibody [NB100-56563] - All the cell lines expressed mRNA for TLR1 (Figure 1) and displayed a strong expression of TLR1 protein (Figure 2). Cell lysates were electrophoresed and blotted to PVDF membrane, which was probed with TLR1-specific antibody. To confirm the immunoreactivity of the antibody, different positive controls were included. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR1-Antibody-Western-Blot-NB100-56563-img0014.jpg)
![Flow Cytometry: TLR1 Antibody [NB100-56563] - Expression of TLR1 in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry. HCMLs were stained using an intracellular staining protocol with TLR1 antibodies followed by relevant secondary fluorescent-conjugated antibodies. Filled histograms (purple) represent the isotype controls and the open histograms (red) indicate TLR1. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR1-Antibody-Flow-Cytometry-NB100-56563-img0015.jpg)
![Immunohistochemistry-Paraffin: TLR1 Antibody [NB100-56563] - analysisof human small intestine using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/TLR1-Antibody-Immunohistochemistry-Paraffin-NB100-56563-img0009.jpg)
![Flow Cytometry: TLR1 Antibody [NB100-56563] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56563. Staining of TLR1 in human PBMCs (lymphocyte-gated) using this antibody at 10 ul/10^6 cells. Shaded histogram represents cells alone, green represents isotype controlwas used for this test.](http://images.novusbio.com/fullsize/TLR1-Antibody-Flow-Cytometry-NB100-56563-img0013.jpg)
![Flow Cytometry: TLR1 Antibody [NB100-56563] - Intracellular analysis of TLR1 AF647 in human PBMCs (lymphocyte-gated) using this antibody at 10 ul/10^6 cells. Shaded histogram represents cells without antibody, green represents isotype control, red represents TLR1 antibody.](http://images.novusbio.com/fullsize/TLR1-Antibody-Flow-Cytometry-NB100-56563-img0012.jpg)
![Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351] - Analysis for RNase L in WT and eIF2a-S51A MEF cell lines transduced with RNase L-encoding lentivirus. n.s. indicates nonspecific band for the purpose of showing equal loading. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31896577/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RNase-L-Antibody-2E9-BSA-Free-Western-Blot-NB100-351-img0010.jpg "Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351] - Analysis for RNase L in WT and eIF2a-S51A MEF cell lines transduced with RNase L-encoding lentivirus. n.s. indicates nonspecific band for the purpose of showing equal loading. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31896577/) licensed under a CC-BY license.")
![Immunohistochemistry: RNase L Antibody (2E9) [NB100-351] - RNase L antibody was tested in human colon using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/RNase-L-Antibody-2E9-Immunohistochemistry-NB100-351-img0006.jpg)
![Western Blot: RNase L Antibody (2E9) [NB100-351] - RNaseL in insect cell extracts transfected with recombinant human RNase L (0.1 ug) and Hey1B (100 ug)](http://images.novusbio.com/fullsize/RNase-L-Antibody-2E9-Western-Blot-NB100-351-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351] - Analysis of PKR and RNase L in parental (WT), RNase L-KO (RL-KO), PKR-KO, and RNase L/PKR double knockout (RL/PKR-KO) A549 cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31896577/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RNase-L-Antibody-2E9-BSA-Free-Knockout-Validated-NB100-351-img0009.jpg)
![Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351] - dsRNA-induced SGs require G3BP1 & G3BP2, whereas RLBs do not.A, immunoblot for G3BP1, RNase L, GAPDH in parental (WT), RL-KO, G3BP-KO, & RL/G3BP-KO U-2 OS cells. B, immunoblot for G3BP1 & G3BP2 in RL-KO & RL/G3BP-KO U-2 OS cells. C, IF for G3BP1 & PABPC1 4 h post-poly(I·C) transfection or treatment with 500 μm sodium arsenite (SA) in the indicated cell lines. D, similar to C but enlarged to show RLB formation in U-2 OS-G3BP1/2-KO cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31896577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-351_mouse-monoclonal-rnase-l-antibody-2e9-310202415171326.jpg)
![Flow (Cell Surface): TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide/BSA FREE version of NBP2-24865. Cell surface analysis of TLR4 on mouse RAW cells using 0.5 ug/10^6 cells of TLR4 antibody, anti-rat APC conjugated secondary antibody and cell surface flow kit. Green: isotype control. Red: TLR4 antibody.](http://images.novusbio.com/fullsize/TLR4-Antibody-MTS510-Flow-Cell-Surface-NB100-56560-img0010.jpg "Flow (Cell Surface): TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide/BSA FREE version of NBP2-24865. Cell surface analysis of TLR4 on mouse RAW cells using 0.5 ug/10^6 cells of TLR4 antibody, anti-rat APC conjugated secondary antibody and cell surface flow kit. Green: isotype control. Red: TLR4 antibody.")
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide Free version of NB100-56560. Cell surface TLR4 in mouse peritoneal cells. Green: isotype control. Red: anti-TLR4 antibody.](http://images.novusbio.com/fullsize/TLR4-Antibody-MTS510-Flow-Cytometry-NB100-56560-img0009.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the FITC conjugate of NBP2-24865. Staining of TLR4 in mouse peritoneal cells using 1 ug of this antibody. Shaded histogram: Cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.](http://images.novusbio.com/fullsize/TLR4-Antibody-MTS510-Flow-Cytometry-NB100-56560-img0012.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the PE conjugate of NBP2-24865. Staining of cell surface TLR4 using this antibody at 0.625 ug/10^6 Raw cells (pretreated with anti-CD16/32). Shaded histogram: isotype control.](http://images.novusbio.com/fullsize/TLR4-Antibody-MTS510-Flow-Cytometry-NB100-56560-img0011.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the PE conjugate of NBP2-24865. Staining of TLR4 in mouse peritoneal cells using 0.5 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.](http://images.novusbio.com/fullsize/nb100-56560_rat-monoclonal-tlr4-antibody-mts510-17720249362619.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the FITC conjugate of NBP2-24865. Staining of TLR4 in RAW cells using 0.5 ug of this antibody. Black histogram represents cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.](http://images.novusbio.com/fullsize/nb100-56560_rat-monoclonal-tlr4-antibody-mts510-17720241003224.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the FITC conjugate of NBP2-24865. Staining of TLR4 in mouse peritoneal cells using 1 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.](http://images.novusbio.com/fullsize/nb100-56560_rat-monoclonal-tlr4-antibody-mts510-17720241003220.jpg)