RNase L Antibody (2E9) [Janelia Fluor® 646]

• Reactivity: Hu, Ha, Pm, Mu(-), Rt(-)
• Applications: WB, ELISA, IHC, KO
• Clone: 2E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 646

RNase L Antibody (2E9) [Janelia Fluor® 646] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Purified full-length recombinant human RNaseL produced in insect cells. [UniProt# Q05823]
• Localization: Cytoplasmic and Mitochondrial.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RNASEL
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB100-351. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

This antibody does not react with mouse or rat.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for RNase L Antibody (2E9) [Janelia Fluor® 646]

• 2-5A-dependent ribonuclease
• 2-5A-dependent RNase
• 2'-5'-oligoisoadenylate synthetase-dependent
• EC 3.1.26.-
• HPC1
• interferon-induced 2-5A-dependent RNase
• MGC104972
• MGC133329
• PRCA1
• prostate cancer 1
• Ribonuclease 4
• ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent)
• Ribonuclease L
• RNase L
• RNS4DKFZp781D08126

Background

RNase L is a virally induced endoribonuclease that is also the terminal factor of the anti-viral action of interferon. Activation of RNase L results in inhibition of viral proliferation through total RNA degradation. Activation of RNase L has also been determined to degrade RNA, thus, initiating a cellular stress response, inducing apoptosis. Since mutations in RNase L relate to an increased incidence of prostate cancer, the activation of RNase L is thought to function in counteracting prostate cancer, via apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNASEL