| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77368-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-39474SS | |||
| Azide and BSA Free | NBP2-80562 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56352SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23669SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-28904SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24779SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-48337SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-48338SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56404SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71770SS | |||
| Azide and BSA Free | NBP2-80904 | |||
![Western Blot: AGTR-2 Antibody [NBP1-77368] - Mouse liver tissue lysate with Angiotensin II Type 2 Receptor antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/AGTR-2-Antibody-Western-Blot-NBP1-77368-img0005.jpg "Western Blot: AGTR-2 Antibody [NBP1-77368] - Mouse liver tissue lysate with Angiotensin II Type 2 Receptor antibody at 0.5 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical stained images demonstrating the expression of SOX2 [(A), red], PRR [(B), red], & ATIIR2 [(C), red] on GFAP+ CSCs [(A–C), green] & expression of ACE [(D), green] & ATIIR1 [(E), green] on SOX2+ CSCs [(D,E), red]. Negative control was a GBM tissue section with omission of the primary antibody (F). Cell nuclei were counterstained with 4′, 6′-diamidino-2-phenylindole [(A–F), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27730123), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-310202415304293.jpg)
![Western Blot: AGTR-2 Antibody - BSA Free [NBP1-77368] - Effects of Ang(1-7) and/or A-779 treatments to the sham & OVX animals for 6 weeks on the expressions of RAS different proteins & osteoclastogenesis modulating factors in the femoral bone heads. (A) Western blot analysis bands showing the expressions of AngII, Ang(1-7), AT1R, AT2R, ACE, ACE-2, MasR, RANKL & OPG. (B) Quantification of the scanning densitometry of the western blot bands (n = 8/group) expressed as arbitrary units. One-way ANOVA test followed by post hoc Student-Newman-Keuls multiple comparisons test were used for the statistical analysis. Columns & bars represent the mean ± SEM of each group. Statistical significance was considered when *P < 0.05 & **P < 0.01 as compared to Sham group & #P < 0.05 & ##P < 0.01 as compared to OVX group. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-02570-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-310202416171482.jpg)
![Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated buccal mucosal squamous cell carcinoma demonstrating PRR [(A), red] was expressed by cells within the tumor nests that stained positively for EMA [(A), green]. PRR [(B), red] was also expressed by the CD34+ [(B), green] endothelium of the microvessels. In addition, RRR [(C), red] was expressed by cells within the peri-tumoral stroma, which also expressed OCT4 [(C), green]. ACE [(D), green] was expressed on the endothelium of the microvessels, which also expressed SOX2 [(D), red], within the peri-tumoral stroma. Cytoplasmic expression of ATIIR1 [(E), green] & PRR [(E), red] was demonstrated in cells within the tumor nests, the peri-tumoral stroma, & the endothelium of the microvessels within the peri-tumoral stroma. ATIIR2 [(F,G), red] was expressed by cells within the tumor nests that expressed SALL4 [(F), green] & OCT4 [(G), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27730124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-31020241616180.jpg)
![Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative 3,3-diaminobenzidine immunohistochemical-stained sections of moderately differentiated lip cancer samples showing cytoplasmic expression of (pro)renin receptor [(A), brown] within the tumor nests (TNs) & cells within the stroma. Angiotensin-converting enzyme [(B), brown] was expressed on the endothelium of the microvessels within the stroma but not the TNs. Angiotensin II receptor 1 [(C), brown] showed weak to moderate cytoplasmic & nuclear staining by cells within the TNs & cells within the stroma. Strong perinuclear & cytoplasmic staining for angiotensin II receptor 2 [(D), brown] was present in cells within the TNs & the stroma, & skeletal tissue. Nuclei were counterstained with hematoxylin [(A–D), blue]. Original magnification: 200×. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fsurg.2017.00030/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-310202416175254.jpg)
![Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated buccal mucosal squamous cell carcinoma demonstrating PRR [(A), red] was expressed by cells within the tumor nests that stained positively for EMA [(A), green]. PRR [(B), red] was also expressed by the CD34+ [(B), green] endothelium of the microvessels. In addition, RRR [(C), red] was expressed by cells within the peri-tumoral stroma, which also expressed OCT4 [(C), green]. ACE [(D), green] was expressed on the endothelium of the microvessels, which also expressed SOX2 [(D), red], within the peri-tumoral stroma. Cytoplasmic expression of ATIIR1 [(E), green] & PRR [(E), red] was demonstrated in cells within the tumor nests, the peri-tumoral stroma, & the endothelium of the microvessels within the peri-tumoral stroma. ATIIR2 [(F,G), red] was expressed by cells within the tumor nests that expressed SALL4 [(F), green] & OCT4 [(G), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27730124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-31020241616562.jpg)
![Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated lip squamous cell carcinoma demonstrating expression of (pro)renin receptor (PRR) [(A,B), red] by cells within the tumor nests (TNs) & the cells within the stroma that expressed SALL4 [(A), green]. PRR was also expressed by cells within the stroma that expressed OCT4 [(B), green]. Angiotensin-converting enzyme (ACE) [(C), green] was expressed on the endothelium of the microvessels which expressed SOX2 [(C), red], within the stroma. Staining with ETS-related gene [(G), red] confirmed that ACE [(G), green] was expressed by the endothelium of the microvessels within the stroma. Angiotensin II receptor 1 [(D), green] was demonstrated in cells within the TNs & occasional microvessels that stained positively for SOX2 [(D), red]. Angiotensin II receptor 2 [(E,F), red] was expressed by cells within the TNs, & cells within the stroma that expressed SALL4 [(E), green] & those that expressed OCT4 [(F), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 µm. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fsurg.2017.00030/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-310202416163773.jpg)
![Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunocytochemistry of AGTR-2 in Jurkat cell with AGTR-2 Antibody at 1 u/ml.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-271120241932624.gif)
![Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunofluorescence of AGTR-2 in Jurkat cell with AGTR-2 Antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-2711202419353826.gif)
![Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunohistochemistry of AGTR-2 in rat liver tissue with AGTR-2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77368_rabbit-polyclonal-agtr-2-antibody-271120242045681.jpg)
![Western Blot: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Adenosine A2a R (7F6-G5-A2) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Western-Blot-NBP1-39474-img0003.jpg "Western Blot: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Adenosine A2a R (7F6-G5-A2) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunocytochemistry-Immunofluorescence-NBP1-39474-img0005.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Staining of human brain (putamen), antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0002.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-87 MG cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunocytochemistry-Immunofluorescence-NBP1-39474-img0004.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Analysis of a FFPE tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at 1:100 dilution. The antibody generated nice membranous / punctate staining of Adenosine A2a receptors.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0006.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Analysis of a FFPE tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at 1:400 dilution. The antibody generated nice membranous / punctate staining of Adenosine A2a receptors.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0008.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on hPBMCs with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474 and a matched isotype control NBP2-14864. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0009.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Using the PE direct conjugate An intracellular stain was performed on SH-SY5Y cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474PE (blue) and a matched isotype control NB600-986PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin (PE).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0011.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0012.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin (PE).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0013.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin (APC).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0014.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on SH-SY5Y with Adenosine A2a R Antibody (7F6-G5-A2) NBP1-39474 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (R&D Systems, F0102B).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0015.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0016.jpg)
![Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.](http://images.novusbio.com/fullsize/5-HT7-Antibody-Western-Blot-NB100-56352-img0003.jpg "Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.")
![Simple Western: 5-HT7 Antibody [NB100-56352] - Time-dependent effects of subacute administration of effective dose of escitalopram (5 mg/kg/day) for 3 & 7 days on expression of 5-HT7R in the thalamic plasma membrane fraction (Panel (A)). Ordinate: mean ± SD (n = 6) of the relative protein level of 5-HT7R in the thalamic plasma membrane fraction. Panel (B) indicates the pseudo-gel images using capillary immunoblotting. ** p < 0.01 vs. control by one-way analysis of variance with Tukey’s post-hoc test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33572981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56352_rabbit-polyclonal-5-ht7-antibody-310202415384177.jpg)
![Western Blot: TGR5/GPBAR1 Antibody [NBP2-23669] - Analysis of spleen lysate. (1) Pre-immune sera. TGR5/GPBAR1 antibody tested on (2) Human 1:1000, (3) Mouse 1:5000, and (4) Rat 1:5000 spleen lysates.](http://images.novusbio.com/fullsize/TGR5-GPBAR1-Antibody-Western-Blot-NBP2-23669-img0008.jpg "Western Blot: TGR5/GPBAR1 Antibody [NBP2-23669] - Analysis of spleen lysate. (1) Pre-immune sera. TGR5/GPBAR1 antibody tested on (2) Human 1:1000, (3) Mouse 1:5000, and (4) Rat 1:5000 spleen lysates.")
![Immunocytochemistry/Immunofluorescence: TGR5/GPBAR1 Antibody [NBP2-23669] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TGR5/GPBAR1 Antibody NBP2-23669 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TGR5-GPBAR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-23669-img0013.jpg)
![Immunohistochemistry-Paraffin: TGR5/GPBAR1 Antibody [NBP2-23669] - Tissue section of mouse colon using TGR5/GPBAR1 antibody at 1:200 dilution. The signal was detected using HRP-labelled secondary antibody and DAB staining which followed counterstaining with hematoxylin. This TGR5/GPBAR1 antibody generated a very specific a cytoplasmic staining primarily in the columner epithelial cells. A subset of cells from mucosa muscularis and the lamina propria also showed TGR5 positivity. Some cells in all histological layers depicted a nuclear staining also.](http://images.novusbio.com/fullsize/TGR5-GPBAR1-Antibody-Immunohistochemistry-Paraffin-NBP2-23669-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: TGR5/GPBAR1 Antibody [NBP2-23669] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TGR5/GPBAR1 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TGR5-GPBAR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-23669-img0011.jpg)
![Western Blot: TGR5/GPBAR1 Antibody - BSA Free [NBP2-23669] - LCA-induced oxidative stress responses were mediated by TGR5 & by CAR receptors. (A) The 4T1 cells were treated with 0.3 μm LCA & NF449, CINPA1, DY268, or GSK2033 at a final concentration of 5 μm for 48 h, then NRF2 protein expression was detected using western blotting (representative figure, n = 2). (B,C) TGR5, CAR, VDR, & PXR bile acid receptors were silenced in MCF7 cells by transiently transfecting the cells with the corresponding siRNA or a negative control siRNA. After 48 h, protein expressions of (B,C) NRF2 & (C) iNOS were determined by western blotting (n = 3). Data are plotted as mean ± SEM. * p < 0.05, control vs. LCA/siRNA treated. (CAR, constitutive androstane receptor; FXR, farnesoid X-activated receptor; LCA, lithocholic acid; LXR, liver X nuclear receptor; NRF2, nuclear factor, erythroid 2-like 2; TGR5/GPBAR1, G protein-coupled bile acid receptor 1/Takeda G-protein coupled receptor; VDR, vitamin D receptor). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31461945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-23669_rabbit-polyclonal-tgr5-gpbar1-antibody-310202416121972.jpg)
![Immunocytochemistry/Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lgr5/GPR49 Antibody NBP1-28904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-28904-img0009.jpg "Immunocytochemistry/Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lgr5/GPR49 Antibody NBP1-28904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Analysis of human small intestine.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-NBP1-28904-img0005.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - BSA Free [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. A, Heat map of 37 genes (shown on the vertical axis), which are 5-fold or greater differentially expressed in ZG & ZF. Genes previously associated with the Wnt signaling pathway are highlighted in red. On the horizontal axis, each pair of ZG & ZF from the 20 adrenals are anonymized & numbered. Probe set & detailed microarray information are available from the National Center for Biotechnology Information Gene Expression Omnibus under accession number GSE64957. B, IHC localization of LGR5 & downstream Wnt signaling proteins in the ZG: from the canonical beta -catenin pathway, beta -catenin & LEF1, & from the noncanonical AP1/JUN pathway, c-JUN & p-c-JUN. IHC was performed on formalin-fixed, paraffin-embedded human adrenal sections (4 μm) using a chromogen-based detection system (3,3'-Diaminobenzidine), which results in a positive brown staining. Interestingly, beta -catenin staining was mostly membranous or cytoplasmic (rather than nuclear staining as highlighted by the arrows), indicating limited canonical Wnt activation. Pictures are representative of six normal adrenal sections that were used in our microarray study; four from primary hyperaldosteronism patients & two from pheochromocytoma patients. Scale bar, 500 μm (low power) & 100 μm (high power). C, capsule. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-1734), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28904_rabbit-polyclonal-lgr5-gpr49-antibody-31020241618943.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IF analysis of Opsin 1 in paraffin embedded and frozen mouse retina tissues. Image courtesy of product review submitted by Linda Vuong.](http://images.novusbio.com/fullsize/Opsin-1-Medium-Wave-Antibody-Immunohistochemistry-Paraffin-NB110-74730-img0001.jpg)
![Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730] - Retinal whole mount of BALB/c mouse stained against anti M-opsin secondary (AF647) antibody and peanut agglutinin (Fluorescein). This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Opsin-1-Medium-Wave-Antibody-Immunohistochemistry-NB110-74730-img0004.jpg)
![Simple Western: CXCR7/RDC-1 Antibody [NBP2-24779] - Lane view shows a specific band for CXCR7 in 0.5 mg/mL of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CXCR7-RDC-1-Antibody-Simple-Western-NBP2-24779-img0003.jpg "Simple Western: CXCR7/RDC-1 Antibody [NBP2-24779] - Lane view shows a specific band for CXCR7 in 0.5 mg/mL of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody [NBP2-24779] - Analysis of a FFPE tissue section of human tonsil using 5 ug/mL concentration of CXCR7/RDC-1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This CXCR7 antibody generated a specific membrane cytoplasmic staining in most of the cells, and the signal was highest/very intense in the endothelial cells/blood vessels.](http://images.novusbio.com/fullsize/CXCR7-RDC-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24779-img0006.jpg)
![Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody [NBP2-24779] - Analysis of a FFPE tissue section of human tonsil using 5 ug/mL concentration of CXCR7/RDC-1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This CXCR7 antibody generated a specific membrane cytoplasmic staining in most of the cells, and the signal was highest/very intense in the endothelial cells/blood vessels.](http://images.novusbio.com/fullsize/CXCR7-RDC-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24779-img0007.jpg)
![Western Blot: CXCR7/RDC-1 Antibody [NBP2-24779] - Total protein from human spleen, tonsil, lymph node and mouse spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-CXCR7 in 1% BSA in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CXCR7-RDC-1-Antibody-Western-Blot-NBP2-24779-img0008.jpg)
![Western Blot: CCR2 Antibody [NBP1-48337] - Analysis of CCR2 in rat lysate. Image courtesy of product review submitted by Rasha Elbaz.](http://images.novusbio.com/fullsize/CCR2-Antibody-Western-Blot-NBP1-48337-img0005.jpg)
![Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48337] - An intracellular stain was performed on U2-OS cells with CCR2 NBP1-48337AF647 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CCR2-Antibody---BSA-Free-Flow-Cytometry-NBP1-48337-img0020.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - Analysis of FFPE tissue section of human liver cancer using 5 ug/ml concentration of CCR2 antibody. Intense cytoplasmic-membranous immune-staining was observed in the cancerous hepatocytes, whereas the staining was relatively weak in the cells adjacent to the fibrotic areas. [Magnification 40X]](http://images.novusbio.com/fullsize/CCR2-Antibody-Immunohistochemistry-Paraffin-NBP1-48337-img0011.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - Analysis of FFPE tissue section of human rectal adenocarcinoma using 5 ug/ml concentration of CCR2 antibody. The cancerous cells in the section developed a uniform but specific CCR2 immunopositivity whereas a weak staining was seen in the tumor stroma cells. [Magnification 40X]](http://images.novusbio.com/fullsize/CCR2-Antibody-Immunohistochemistry-Paraffin-NBP1-48337-img0012.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - Analysis of FFPE section of human esophageal squamous cell carcinoma (SCC) using 5 ug/ml concentration of CCR2 antibody. Strong cytoplasmic-membranous immune-staining was observed in SCC cells and a relatively weaker staining was seen in the tumor stroma cells. [Magnification 10X]](http://images.novusbio.com/fullsize/CCR2-Antibody-Immunohistochemistry-Paraffin-NBP1-48337-img0013.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - Analysis of FFPE tissue section of human normal kidney using 5 ug/ml concentration of CCR2 antibody. Strong cytoplasmic immunostaining was observed in the various renal cells (especially from tubules/ducts epithelium). The cells of Bowman's capsule depicted a very weak cytoplasmic staining for CCR2 protein. [Magnification 10X]](http://images.novusbio.com/fullsize/CCR2-Antibody-Immunohistochemistry-Paraffin-NBP1-48337-img0014.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - Analysis of FFPE tissue section of human normal breast using 5 ug/ml concentration of CCR2 antibody. The breast ductal/acinar epithelial cells showed a strong cytoplasmic-membranous CCR2 immune-positivity, whereas the intra-lobular connective tissue depicted weak staining. [Magnification 40X]](http://images.novusbio.com/fullsize/CCR2-Antibody-Immunohistochemistry-Paraffin-NBP1-48337-img0015.jpg)
![Flow Cytometry: CCR2 Antibody [NBP1-48337] - An intracellular stain was performed on THP-1 cells with CCR Antibody NBP1-48337R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/fullsize/CCR2-Antibody-Flow-Cytometry-NBP1-48337-img0016.jpg)
![Flow Cytometry: CCR2 Antibody [NBP1-48337] - An intracellular stain was performed on THP-1 cells with CCR2 Antibody NBP1-48337 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CCR2-Antibody-Flow-Cytometry-NBP1-48337-img0018.jpg)
![Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48337] - An intracellular stain was performed on U2-OS cells with CCR2 NBP1-48337 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CCR2-Antibody---BSA-Free-Flow-Cytometry-NBP1-48337-img0019.jpg)
![Western Blot: GLP-1R Antibody [NLS1205] - WB detection of GLP-1R in lysates of human (1) pancreas (2) lung and (3) kidney tissues using 2 ug/mL concentration of GLP-1R antibody.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Western-Blot-NLS1205-img0012.jpg)
![Immunohistochemistry: GLP-1R Antibody [NLS1205] - Analysis of GLP-1R in mouse pancreas.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-NLS1205-img0010.jpg)
![Immunohistochemistry: GLP-1R Antibody [NLS1205] - Human pancreas (Islets of Langerhans).](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-NLS1205-img0008.jpg)
![Simple Western: GLP-1R Antibody [NLS1205] - Image shows a specific band for GLP-1R in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Simple-Western-NLS1205-img0005.jpg)
![Microarray: GLP-1R Antibody [NLS1205] - Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. Microarray image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Microarray-NLS1205-img0013.jpg)
![Simple Western: GLP-1R Antibody - BSA Free [NLS1205] - Effect of the administration of exendin‐4 on eNOS expression. (a) Capillary western blot of total eNOS & phosphorylated eNOS expression in the rat retina. The red box indicates the target protein. (b & c) Fold change in the expression of total eNOS (b) & phosphorylated eNOS (c) in the rat retina (n = 7 in control group; n = 6 in Hp + PBS group; n = 9 in other groups). (d & e) Capillary western blot & fold change in the expression of GLP‐1 receptors (GLP‐1R) (d), PI3K, & Akt (e) in the rat retina. *P < .05, significantly different from CSF group; #P < .05significantly different from noradrenaline without exendin‐4 group. (f) NO content in human retinal microvascular endothelial cells (n = 6, 5, 5, 6, 7, 5, & 6 for group of control, OGD, OGD + Ex‐4, OGD + Ex‐4 + Ex‐9–39‐L, OGD + Ex‐4 + Ex‐9–39‐H, OGD + Ex‐4 + l‐NAME‐L, & OGD + Ex‐4 + l‐NAME‐H, respectively). One‐way ANOVA with LSD or Dunnett's T3 test were performed. C, control group; HP, high pressure injury group; EX‐4, exendin‐4; s.c., subcutaneous injection of exendin‐4; i.v., intravitreal injection of exendin‐4; od, eye drops of exendin‐4; OGD, oxygen glucose deprivation model; EX‐9–39‐L, low concentration of exendin‐9‐39 (10 nM); EX‐9–39‐H, high concentration of exendin‐9‐39 (20 nM); l‐NAME‐l, low concentration of l‐NAME (50 μM); l‐NAME‐H, high concentration of l‐NAME (100 μM) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32232832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls1205_rabbit-polyclonal-glp-1r-antibody-3102024161368.jpg)
![Flow Cytometry: CCR2 Antibody [NBP1-48338] - An intracellular stain was performed on THP-1 cells with CCR2 Antibody NBP1-48338G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.](http://images.novusbio.com/fullsize/CCR2-Antibody-Flow-Cytometry-NBP1-48338-img0004.jpg "Flow Cytometry: CCR2 Antibody [NBP1-48338] - An intracellular stain was performed on THP-1 cells with CCR2 Antibody NBP1-48338G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.")
![Immunocytochemistry/Immunofluorescence: CCR2 Antibody [NBP1-48338] - Analysis of CCR2 in NIH/3T3 cells.](http://images.novusbio.com/fullsize/CCR2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-48338-img0003.jpg)
![Flow Cytometry: CCR2 Antibody [NBP1-48338] - An intracellular stain was performed on THP-1 cells with CCR2 Antibody NBP1-48338F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/CCR2-Antibody-Flow-Cytometry-NBP1-48338-img0006.jpg)
![Flow Cytometry: CCR2 Antibody [NBP1-48338] - The Alexa Fluor 488 conjugate of this antibody was used: Mouse peripheral blood mononuclear cells were unstained (left) or stained (right) with CCR2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CCR2-Antibody-Flow-Cytometry-NBP1-48338-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: CCR2 Antibody - BSA Free [NBP1-48338] - Expression patterns of CCL2 & CCR2 in unwounded & wounded corneas of WT & MMP12 KO mice. (A) Immunofluorescence of CCL2 chemokine & (B) its receptor CCR2 in unwounded & chemically wounded (2-days after injury) WT & Mmp12−/− mouse corneas. Control images represent mouse corneas stained with secondary antibody only & without primary antibody. Nuclei were visualized by staining with DAPI (blue). Scale bars: 50 µm. A magnified image of a wounded WT cornea shows perinuclear expression of CCL2 (orange box). CCL2 staining was visualized in epithelial, stromal, & endothelial layers of wounded WT & Mmp12−/− corneas. CCR2 staining was visualized in epithelial & stromal layers of unwounded & wounded WT & Mmp12−/− corneas. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31399604), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-48338_rabbit-polyclonal-ccr2-antibody-310202415175260.jpg)
![Western Blot: CXCR3 Antibody [NB100-56404] - HEK293T cells. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Western-Blot-NB100-56404-img0004.jpg "Western Blot: CXCR3 Antibody [NB100-56404] - HEK293T cells. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: CXCR3 Antibody [NB100-56404] - Brain microvascular endothelial cells isolated from WT and CXCR3 KO mice were stained with anti-CXCR3 (green) and anti-ZO-1 (blue) antibodies to analyze CXCR3 expression by immunofluorescence. Image from verified customer review.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56404-img0006.jpg)
![Immunohistochemistry-Paraffin: CXCR3 Antibody [NB100-56404] - IHC analysis of a FFPE human spleen using 1:25 dilution of CXCR3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of HIER using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (40X) was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Immunohistochemistry-Paraffin-NB100-56404-img0005.jpg)
![Western Blot: CXCR3 Antibody [NB100-56404] - Analysis of CXCR3 in human pancreas lysate using CXCR3 antibody at 2 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Western-Blot-NB100-56404-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: PAR1/Thrombin Receptor Antibody (N2-11) [NBP1-71770] - Analysis of Thrombin Receptor expression in human testis using NBP1-71770.](http://images.novusbio.com/fullsize/PAR1-Thrombin-Receptor-Antibody-N2-11-Western-Blot-NBP1-71770-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: PAR1/Thrombin Receptor Antibody (N2-11) [NBP1-71770] - Thrombin Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/PAR1-Thrombin-Receptor-Antibody-N2-11-Immunocytochemistry-Immunofluorescence-NBP1-71770-img0011.jpg)
![Western Blot: PAR1/Thrombin Receptor Antibody (N2-11) [NBP1-71770] - Analysis of PAR1 in whole cell lysate of Neuro2A cell line using 4ug/ml concentration of PAR1 antibody (clone N2-11). The antibody detected a strong band at ~60kDa position and another light band at ~80kDa, both of which appears to be the glycosylated forms of PAR1 protein.](http://images.novusbio.com/fullsize/PAR1-Thrombin-Receptor-Antibody-N2-11-Western-Blot-NBP1-71770-img0010.jpg)
![Immunohistochemistry: PAR1/Thrombin Receptor Antibody (N2-11) [NBP1-71770] - Analysis of Thrombin Receptor on mouse skin using NBP1-71770.](http://images.novusbio.com/fullsize/PAR1-Thrombin-Receptor-Antibody-N2-11-Immunohistochemistry-NBP1-71770-img0012.jpg)
![Immunocytochemistry/ Immunofluorescence: PAR1/Thrombin Receptor Antibody (N2-11) [NBP1-71770] - PAR1 is expressed by neural stem cells in the sub-ventricular zone (SVZ) of the adult mouse brain. Photomicrographs show immunofluorescence double-labeling for PAR1 with Sox2-positive (A), or PAR1 with Nestin-positive (C) multipotent neural stem cells (NSCs) within the lateral wall of the lateral ventricle (LV). RNAscope was used to identify cells expressing both PAR1 & Sox2 (B), or Nestin (D) RNA in NSCs of the adult SVZ. Arrow indicates an example of a double-labeled cell in each case, with arrowhead indicating a singly labeled cell (Scale bar = 10 μm). Boxed area in B & D is also shown at higher magnification to visualize double-labeled cells. (E) Histogram shows expression of PAR1 RNA was high in NSCs grown as neurospheres (NS), or when plated on poly-L-lysine coated coverslips as monolayers in stem cell media containing EGF & bFGF. PAR1 RNA expression by NSC monolayers decreased by 87% when EGF & bFGF were removed from the media for 7 DIV promoting stem cell differentiation. (F) Withdrawal of EGF & bFGF to induce NSC monolayer differentiation resulted in a parallel decrease in Nestin RNA expression. (**P < 0.01, ***P < 0.001 Students t-test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29921916), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-71770_mouse-monoclonal-par1-thrombin-receptor-antibody-n2-11-3102024161893.jpg)