PAR1/Thrombin Receptor Antibody (N2-11) [HRP]

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC, IHC-Fr, IHC-P
• Clone: N2-11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: HRP

PAR1/Thrombin Receptor Antibody (N2-11) [HRP] Summary

• Immunogen: The oligopeptide CNATLDPRSFLL from human Thrombin Receptor. [Swiss-Prot# P25116]
• Localization: Cell membrane; Multi-pass membrane protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: F2R
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This Thrombin Receptor (N2-11) antibody is useful for ELISA, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~52 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PAR1/Thrombin Receptor Antibody (N2-11) [HRP]

• Cf2r
• CF2RHTR
• coagulation factor II (thrombin) receptor
• Coagulation factor II receptor
• F2R
• HTR
• PAR1
• PAR1PAR-1protease-activated receptor 1
• Protease-Activated Receptor 1
• Thrombin receptor
• ThrR
• TRGPC
• TRproteinase-activated receptor 1

Background

Thrombin Receptor, also known as Protease activated receptor 1 (PAR1), is a G-protein-coupled receptor that is highly expressed in invasive cancers. While Thrombin Receptor is known to have important functions in wound healing, by inducing human platelet recruitment to form stable blood clots for vascular injury, its misregulation seems to be intrinsically linked to the formation of invasive and deadly cancers. Thrombin Receptor is important in the cell invasion of cutaneous malignant melanoma, along with its partner KLK6. In lung cancer, Thrombin Receptor migration pairs with ERK1/2 activity to induce cell migration and tumor growth. Thrombin Receptor is known to interact with FAK, which alters endothelial barriers which normally would inhibit cell proliferation across boundaries. It is probably through this relationship that many metastatic effects associated with Thrombin Receptor are compounded. Thrombin Receptor normally functions to assist with healing, particularly in vasculature, but when it is not appropriately regulated, it can catalyze cancer growth and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: F2R