Adenosine A2aR Antibody (7F6-G5-A2) - BSA Free

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Western Blot: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane ...read more
Immunocytochemistry/ Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. ...read more
Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Staining of human brain (putamen), antibody at 5 ug/mL.
Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-87 MG cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control ...read more
Immunocytochemistry/ Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton ...read more
Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Analysis of a FFPE tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at 1:100 dilution. The antibody ...read more
Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Analysis of a FFPE tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at 1:400 dilution. The antibody ...read more
Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on hPBMCs with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474 and a matched isotype control NBP2-14864. ...read more
Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Using the PE direct conjugate An intracellular stain was performed on SH-SY5Y cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474PE (blue) and a ...read more
Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474 and a matched isotype control. Cells were ...read more
Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474PE (blue) and a matched isotype control ...read more
Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474APC (blue) and a matched isotype control ...read more
Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on SH-SY5Y with Adenosine A2a R Antibody (7F6-G5-A2) NBP1-39474 and a matched isotype control. Cells were fixed ...read more
Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Gp, Rb, ShSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
7F6-G5-A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Adenosine A2aR Antibody (7F6-G5-A2) - BSA Free Summary

Immunogen
Full length recombinant human Adenosine A2a R. [UniProt# P29274]
Epitope
Epitope mapped to SQPLPGER in the third intracellular loop (PMID 9822147).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ADORA2A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular) 1 - 5 ug/ml
  • Flow Cytometry 1-5 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1 -5 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1 -5 ug/ml
  • Western Blot 1:1000
Application Notes
ICC usage is reported in scientific literature (PMID: 25836920). Use in IHC-frozen sections reported in scientific literature (PMID 9822147).
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-39474 in the following applications:

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 29791204). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Adenosine A2aR Antibody (7F6-G5-A2) - BSA Free

  • A2aR
  • adenosine A2 receptor
  • Adenosine A2a R
  • adenosine A2a receptor
  • Adenosine A2aR
  • adenosine receptor A2a
  • adenosine receptor subtype A2a
  • ADORA 2
  • ADORA2
  • ADORA2A
  • hA2aR
  • RDC8

Background

This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-22203
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NBP1-47704
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Publications for Adenosine A2aR Antibody (NBP1-39474)(14)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Porcine, Sheep.

We have publications tested in 7 applications: FLOW, Flow, ICC, ICC/IF, IHC, IHC-Fr, WB.


Filter By Application
FLOW
(2)
Flow
(1)
ICC
(1)
ICC/IF
(2)
IHC
(1)
IHC-Fr
(1)
WB
(6)
All Applications
Filter By Species
Human
(7)
Mouse
(2)
Rat
(2)
Porcine
(1)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NBP1-39474 Applications Species
Davis L, Musso J, Soman D et al. ROLE OF ADENOSINE SIGNALING IN COORDINATING CARDIOMYOCYTE FUNCTION AND CORONARY VASCULAR GROWTH IN CHRONIC FETAL ANEMIA Am. J. Physiol. Regul. Integr. Comp. Physiol. May 23 2018 [PMID: 29791204] (IHC, Sheep) IHC Sheep
Chimote AA, Balajthy A, Arnold MJ, et al. A defect in KCa3.1 channel activity limits the ability of CD8+ T cells from cancer patients to infiltrate an adenosine-rich microenvironment. Sci Signal. 2018 Apr 24 [PMID: 29692361] (FLOW, Human) FLOW Human
Hirata Y, Furuhashi K, Ishii H et al. CD150(high) Bone Marrow Tregs Maintain Hematopoietic Stem Cell Quiescence and Immune Privilege via Adenosine Cell Stem Cell. 2018 Mar 1 [PMID: 29456159]

Details:
Citation using the Alexa Fluor 647 form of this antibody.
Mediavilla-Varela M, Castro J, Chiappori A et al. A Novel Antagonist of the Immune Checkpoint Protein Adenosine A2a Receptor Restores Tumor-Infiltrating Lymphocyte Activity in the Context of the Tumor Microenvironment. Neoplasia. 2017 Jun 02 [PMID: 28582704] (Flow, Human) Flow Human
Del Ry S, Moscato S, Bianchi F et al. Altered expression of connexin 43 and related molecular partners in a pig model of left ventricular dysfunction with and without dipyrydamole therapy Pharmacol. Res. 2015 Mar 30 [PMID: 25836920] (ICC/IF, Rat) ICC/IF Rat
Nguyen KD. Inflammatory Cytokines Regulate Adenosine A2A Receptor Expression, Function, and Desensitization. Book from NEW YORK UNIVERSITY. 2007
Baum N, Fliegert R, Bauche A, et al. Daratumumab and Nanobody-Based Heavy Chain Antibodies Inhibit the ADPR Cyclase but not the NAD+ Hydrolase Activity of CD38-Expressing Multiple Myeloma Cells Cancers Dec 30 2020 [PMID: 33396591] (Human) Human
Basu, M, Gupta, P Et al. Increased host ATP efflux and its conversion to extracellular adenosine is crucial for establishing Leishmania infection. J Cell Sci Apr 8 2020 [PMID: 32079656] (WB, Human) WB Human
Clancy-Thompson E, Perekslis TJ, Croteau W et al. Melanoma Induces, and Adenosine Suppresses, CXCR3-Cognate Chemokine Production and T-cell Infiltration of Lungs Bearing Metastatic-like Disease. Cancer Immunol Res. 2015 Aug 1 [PMID: 26048575] (FLOW, Mouse, Human)

Details:
Adenosine A2a R antibody (clone 7F6-G5-A2) was used for FLOW analysis of adenosine receptor A2A (ADORA2A) in murine B16 melanoma and human VMM12 melanoma cells (Figure 7).
FLOW Mouse, Human
Ribeiro AS, Fernandes VS, Orensanz LM, Martinez MP, Recio P, Martinez-Saenz A, Climent B, Arteaga JL, Garcia-Sacristan A, Prieto D, Hernandez M. Mechanisms involved in the adenosine-induced vasorelaxation to the pig prostatic small arteries. Purinergic Signal;7(4):413-25. 2011 Dec. [PMID: 21567127] (WB, Porcine) WB Porcine
Show All 14 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Adenosine A2aR Antibody (NBP1-39474). (Showing 1 - 1 of 1 FAQs).

  1. What is the  concentration of Adenosine A2a R Antibody (7F6-G5-A2) [Alexa Fluor® 647]
    • We routinely use 0.1 mg of base antibodies (i.e. unconjugated antibody like NBP1-39474) in our conjugation reactions with the dyes/enzymes of interest. And the recovery rates mostly fall between 85 to 100% and hence the concentrations are about 0.80 - 1.0 mg/ml, in 0.1 ml volume.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ADORA2A
Entrez
Uniprot