| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-549-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-35334SS | |||
| Azide and BSA Free | NBP2-80623 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24918SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31374SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-26691SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-157SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NLS932AF405 | |||
| Alexa Fluor 488 | NLS932AF488 | |||
| Alexa Fluor 647 | NLS932AF647 | |||
| Alexa Fluor 700 | NLS932AF700 | |||
| Biotin | NLS932B | |||
| DyLight 405 | NLS932V | |||
| DyLight 488 | NLS932G | |||
| DyLight 550 | NLS932R | |||
| DyLight 650 | NLS932C | |||
| FITC | NLS932F | |||
| HRP | NLS932H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56485SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80607 | |||
| BSA Free | NB100-1830SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24503SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-81586SS | |||
![Western Blot: Adenosine A1R Antibody [NB300-549] - Analysis was performed on whole cell extracts (30 ug lysate) of PC 12 (Lane 1), SH-SY5Y (lane 2), U-87MG (lane 3), A549 (Lane 4) and K-562 (lane 5).](http://images.novusbio.com/fullsize/Adenosine-A1-R-Antibody-Western-Blot-NB300-549-img0008.jpg "Western Blot: Adenosine A1R Antibody [NB300-549] - Analysis was performed on whole cell extracts (30 ug lysate) of PC 12 (Lane 1), SH-SY5Y (lane 2), U-87MG (lane 3), A549 (Lane 4) and K-562 (lane 5).")
![Immunocytochemistry/Immunofluorescence: Adenosine A1R Antibody [NB300-549] - Analysis of Adenosine Receptor A1 was done on 70% confluent log phase HeLa cells.](http://images.novusbio.com/fullsize/Adenosine-A1-R-Antibody-Immunocytochemistry-Immunofluorescence-NB300-549-img0007.jpg)
![Immunohistochemistry-Paraffin: Adenosine A1R Antibody [NB300-549] - Analysis showing positive staining in the cytoplasm of Rat brain tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Adenosine-A1-R-Antibody-Immunohistochemistry-Paraffin-NB300-549-img0003.jpg)
![Flow Cytometry: Adenosine A1R Antibody [NB300-549] - Analysis of Adenosine Receptor A1 was done on A549 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Adenosine Receptor A1 Rabbit Polyclonal Antibody (PA1041A, red histogram) or with rabbit isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor (R) 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune (R) Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.](http://images.novusbio.com/fullsize/Adenosine-A1-R-Antibody-Flow-Cytometry-NB300-549-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine A1R Antibody [NB300-549] - Dissociated rat hippocampal culture. Image courtesy of Janosch Heller, University College London.](http://images.novusbio.com/fullsize/Adenosine-A1-R-Antibody-Immunocytochemistry-Immunofluorescence-NB300-549-img0002.jpg)
![Western Blot: CCR2 Antibody (3G7) [NBP2-35334] - Western blot analysis of CCR2 [3G7] in Molt4 lysate.](http://images.novusbio.com/fullsize/CCR2-Antibody-3G7-Western-Blot-NBP2-35334-img0007.jpg "Western Blot: CCR2 Antibody (3G7) [NBP2-35334] - Western blot analysis of CCR2 [3G7] in Molt4 lysate.")
![Immunohistochemistry-Paraffin: CCR2 Antibody (3G7) [NBP2-35334] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast using CCR2 antibody (clone 3G7) at 5 ug/ml concentration. The glandular cells in the lobules showed a specific cytoplasmic-membranous immunostaining of CCR2.](http://images.novusbio.com/fullsize/CCR2-Antibody-3G7-Immunohistochemistry-Paraffin-NBP2-35334-img0006.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody (3G7) [NBP2-35334] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human HCC (hepatocellular carcinoma / malignant hepatoma) using CCR2 antibody (clone 3G7) at 5 ug/ml concentration. The cancer cells depicted specific punctate to diffused cytoplasmic-membranous immuno-staining of CCR2 protein.](http://images.novusbio.com/fullsize/CCR2-Antibody-3G7-Immunohistochemistry-Paraffin-NBP2-35334-img0003.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody (3G7) [NBP2-35334] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using CCR2 antibody (clone 3G7) at 5 ug/ml concentration. The cancer cells depicted specific cytoplasmic-membranous immunostaining, whereas the cells of tumor stroma were largely negative for CCR2 immunopositivity.](http://images.novusbio.com/fullsize/CCR2-Antibody-3G7-Immunohistochemistry-Paraffin-NBP2-35334-img0005.jpg)
![Western Blot: GPR18 Antibody [NBP2-24918] - Analysis of human spleen lysate (4 ug/mL) in the 1) absence and 2) presence of immunizing peptide 3) mouse spleen (6 ug/mL) and 4) rat spleen lysate (3 ug/mL) using GPR18 antibody.](http://images.novusbio.com/fullsize/GPR18-Antibody-Western-Blot-NBP2-24918-img0001.jpg "Western Blot: GPR18 Antibody [NBP2-24918] - Analysis of human spleen lysate (4 ug/mL) in the 1) absence and 2) presence of immunizing peptide 3) mouse spleen (6 ug/mL) and 4) rat spleen lysate (3 ug/mL) using GPR18 antibody.")
![Immunocytochemistry/Immunofluorescence: GPR18 Antibody [NBP2-24918] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GPR18 Antibody NBP2-24918 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GPR18-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24918-img0006.jpg)
![Immunohistochemistry-Paraffin: GPR18 Antibody [NBP2-24918] - Analysis of a FFPE tissue section of human tonsil using 1:200 dilution of GPR18 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/GPR18-Antibody-Immunohistochemistry-Paraffin-NBP2-24918-img0003.jpg)
![Flow Cytometry: GPR18 Antibody [NBP2-24918] - An intracellular stain was performed on Daudi cells with GPR18 Antibody NBP2-24918 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/GPR18-Antibody-Flow-Cytometry-NBP2-24918-img0007.jpg)
![Immunohistochemistry-Paraffin: GPR18 Antibody [NBP2-24918] - Analysis of human breast tumor tissue using an isotype control (top) and GPR18 antibody (bottom) at 5 ug/mL.](http://images.novusbio.com/fullsize/GPR18-Antibody-Immunohistochemistry-Paraffin-NBP2-24918-img0002.jpg)
![Flow Cytometry: GPR18 Antibody [NBP2-24918] - An intracellular stain was performed on Ntera2 cells with GPR18 Antibody NBP2-24918 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/GPR18-Antibody-Flow-Cytometry-NBP2-24918-img0004.jpg)
![Flow Cytometry: GPR18 Antibody [NBP2-24918] - An intracellular stain was performed on THP-1 cells with GPR18 Antibody NBP2-24918 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/GPR18-Antibody-Flow-Cytometry-NBP2-24918-img0005.jpg)
![Western Blot: CCR5 Antibody [NBP2-31374] - Image of anti-CCR5 antibody. Whole cell protein from Daudi (1), K562 (2) and THP-1 (3) cell lines were separated by SDS-PAGE and protein transfered to PVDF. The membrane was probed with anti-CCR5 antibody at 2 ug/mL and detected with an anti-rabbit-HRP secondary antibody and chemiluminescence. CCR5 is shown at 40 kDa.](http://images.novusbio.com/fullsize/CCR5-Antibody-Western-Blot-NBP2-31374-img0003.jpg "Western Blot: CCR5 Antibody [NBP2-31374] - Image of anti-CCR5 antibody. Whole cell protein from Daudi (1), K562 (2) and THP-1 (3) cell lines were separated by SDS-PAGE and protein transfered to PVDF. The membrane was probed with anti-CCR5 antibody at 2 ug/mL and detected with an anti-rabbit-HRP secondary antibody and chemiluminescence. CCR5 is shown at 40 kDa.")
![Western Blot: CCR5 Antibody [NBP2-31374] - Detection of CCR5 partial recombinant protein by using CCR5 antibody at a concentration of 0.5 ug/mL.](http://images.novusbio.com/fullsize/CCR5-Antibody-Western-Blot-NBP2-31374-img0001.jpg)
![Immunohistochemistry-Paraffin: CCR5 Antibody [NBP2-31374] - Analysis of CCR5 protein in a section of malignant stromal tumor of small bowel from human using CCR5 antibody at a concentration of 5 ug/mL.](http://images.novusbio.com/fullsize/CCR5-Antibody-Immunohistochemistry-Paraffin-NBP2-31374-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR5 Antibody [NBP2-31374] - Staining in a Rhesus macaque brain. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CCR5-Antibody-Immunohistochemistry-Paraffin-NBP2-31374-img0005.jpg)
![Immunohistochemistry-Paraffin: CCR5 Antibody [NBP2-31374] - Human skin section. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CCR5-Antibody-Immunohistochemistry-Paraffin-NBP2-31374-img0006.jpg)
![Simple Western: CCR5 Antibody [NBP2-31374] - Lane view shows a specific band for CCR5 in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CCR5-Antibody-Simple-Western-NBP2-31374-img0004.jpg)
![Immunohistochemistry: CCR5 Antibody [NBP2-31374] - Detection of CCR5 & virus on brain tissue. CCR5 expression was analyzed using Immunohistochemistry on the layer III of frontal cortex from macaques that were a Uninfected & untreated controls, b Meth-only treated, c SIV-only infected, & d SIV-infected & Meth-treated. e SIV-infected & Meth-treated. Pictures were at 16× magnification. Sites of interest (rectangles) were further magnified. f Brain viral load (Student’s t test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27107567), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-31374_rabbit-polyclonal-ccr5-antibody-310202415345331.jpg)
![Immunohistochemistry: CCR5 Antibody [NBP2-31374] - Detection of CCR5 & virus on brain tissue. CCR5 expression was analyzed using Immunohistochemistry on the layer III of frontal cortex from macaques that were a Uninfected & untreated controls, b Meth-only treated, c SIV-only infected, & d SIV-infected & Meth-treated. e SIV-infected & Meth-treated. Pictures were at 16× magnification. Sites of interest (rectangles) were further magnified. f Brain viral load (Student’s t test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27107567), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-31374_rabbit-polyclonal-ccr5-antibody-310202415371940.jpg)
![Immunohistochemistry: CCR5 Antibody [NBP2-31374] - Detection of CCR5 & virus on brain tissue. CCR5 expression was analyzed using Immunohistochemistry on the layer III of frontal cortex from macaques that were a Uninfected & untreated controls, b Meth-only treated, c SIV-only infected, & d SIV-infected & Meth-treated. e SIV-infected & Meth-treated. Pictures were at 16× magnification. Sites of interest (rectangles) were further magnified. f Brain viral load (Student’s t test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27107567), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-31374_rabbit-polyclonal-ccr5-antibody-310202415291975.jpg)
![Immunohistochemistry: CCR5 Antibody [NBP2-31374] - Detection of CCR5 & virus on brain tissue. CCR5 expression was analyzed using Immunohistochemistry on the layer III of frontal cortex from macaques that were a Uninfected & untreated controls, b Meth-only treated, c SIV-only infected, & d SIV-infected & Meth-treated. e SIV-infected & Meth-treated. Pictures were at 16× magnification. Sites of interest (rectangles) were further magnified. f Brain viral load (Student’s t test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27107567), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-31374_rabbit-polyclonal-ccr5-antibody-31020241517527.jpg)
![Immunohistochemistry: CCR5 Antibody [NBP2-31374] - Detection of CCR5 & virus on brain tissue. CCR5 expression was analyzed using Immunohistochemistry on the layer III of frontal cortex from macaques that were a Uninfected & untreated controls, b Meth-only treated, c SIV-only infected, & d SIV-infected & Meth-treated. e SIV-infected & Meth-treated. Pictures were at 16× magnification. Sites of interest (rectangles) were further magnified. f Brain viral load (Student’s t test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27107567), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-31374_rabbit-polyclonal-ccr5-antibody-310202415304245.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4271] - Analysis of anti-GPR30 antibody with human brain, hippocampus.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS4271-img0003.jpg)
![Western Blot: GPER/GPR30 Antibody [NLS4271] - Analysis of anti-GPR30 antibody with rat hypothalamus 5-10ug protein loaded per lane. Image courtesy provided by Carrie McAllister.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Western-Blot-NLS4271-img0002.jpg)
![Western Blot: GPER/GPR30 Antibody - BSA Free [NLS4271] - GPER/GPR30 expression in normal lung cell lines & lung adenocarcinoma cell lines. A) The expression of GPER was determined by realtime quantitative PCR in each of the normal lung cell lines (open bars), lung adenocarcinoma cell lines (grey bars), & MCF-7 human breast cancer cells (black bar). Values are the average of 4–6 biological replicates ± SEM. B-D) Representative western blot analysis of GPER/GPR30 expression in the indicted cell lines. 30 μg of whole cell extract (WCE) were separated on 10% SDS PAGE gels with MW marker migration indicated at the left of each blot. The diagrams at the top of B-C, & D indicate the region of GPER recognized by the 3 different polyclonal antibodies used: B) Novus NBP-1-31239, C) Novus NLS4271; D) Santa Cruz sc-4854-R. The MW of GPR30 is estimated to be 42 kDa, but higher MW sizes have been reported due to glycosylation & interaction with other proteins. Bands are identified as glycosylated & nonglycosylated based on reports cited in the text, but could include interaction with other proteins. For each GPER western, the membrane was stripped & reprobed for beta -actin as a loading control. Quantitation of GPER was evaluated by summing all immunoreactive bands & dividing by beta -actin, then normalizing to HBEC2-KT in each blot. Panel E is a summary of all westerns (not all westerns are shown) with each antibody. For Westerns with NBP1-31239 & sc-48524, values are the mean ± SEM from 3 separate blots using different WCE. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23273253), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls4271_rabbit-gper-gpr30-pab-bsa-free-31020241613630.jpg)
![Western Blot: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Analysis of S1P2/Edg5 in heart lysate; Lane 1 shows pre-immune sera; Lanes 2, 3 and 4 show this antibody tested on human heart (1 ug/ml), mouse heart (1 ug/ml) and rat heart (2 ug/ml) lysate, respectively.](http://images.novusbio.com/fullsize/S1P2-EDG-5-S1PR2-Antibody-Western-Blot-NBP2-26691-img0002.jpg "Western Blot: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Analysis of S1P2/Edg5 in heart lysate; Lane 1 shows pre-immune sera; Lanes 2, 3 and 4 show this antibody tested on human heart (1 ug/ml), mouse heart (1 ug/ml) and rat heart (2 ug/ml) lysate, respectively.")
![Immunocytochemistry/Immunofluorescence: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Human PC3 prostate cancer cell line. Primary antibody NBP2-26691 at 1:200, secondary antibody Alexa488, Hoechst 33342 used for nuclear staining. Image from verified customer review.](http://images.novusbio.com/fullsize/S1P2-EDG-5-S1PR2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-26691-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Somatostatin R2/SSTR2 Antibody [NB300-157] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 1:50 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Somatostatin-R2-SSTR2-Antibody-Immunocytochemistry-Immunofluorescence-NB300-157-img0005.jpg "Immunocytochemistry/Immunofluorescence: Somatostatin R2/SSTR2 Antibody [NB300-157] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 1:50 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157] - Staining of Somatostatin Receptor 2 in mouse pancreas using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Somatostatin-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NB300-157-img0004.jpg)
![Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157] - Staining of paraffin embedded rat pancreas tissue.](http://images.novusbio.com/fullsize/Somatostatin-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NB300-157-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NLS932] - Melatonin receptor type 1 B (MTR1B) in rat tissues fixed in paraformaldehyde and embedded in paraffin. A,B-brain, C-prostate. Image from verified customer review.](http://images.novusbio.com/fullsize/Melatonin-Receptor-1B-Antibody-Immunohistochemistry-Paraffin-NLS932-img0008.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NLS932] - Tissue localization of melatonin receptor type 1B (MTR1B) in the ventral prostate of rats. Short-term diabetic treated with MLT (MD1) Influence of Melatonin on the Proliferative and Apoptotic Responses of the Prostate under Normal and Hyperglycemic Conditions. <i>J Diabetes Res</i> (2015)](http://images.novusbio.com/fullsize/Melatonin-Receptor-1B-Antibody-Immunohistochemistry-Paraffin-NLS932-img0009.jpg)
![Western Blot: Melatonin Receptor 1B Antibody [NLS932] - Analysis of 1) HepG2 and 2) A431.](http://images.novusbio.com/fullsize/Melatonin-Receptor-1B-Antibody-Western-Blot-NLS932-img0003.jpg)
![Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NLS932] - Tissue localization of melatonin receptor type 1B (MTR1B) in the ventral prostate of rats. Short-term control (C1). Influence of Melatonin on the Proliferative and Apoptotic Responses of the Prostate under Normal and Hyperglycemic Conditions. <i>J Diabetes Res</i> (2015)](http://images.novusbio.com/fullsize/Melatonin-Receptor-1B-Antibody-Immunohistochemistry-Paraffin-NLS932-img0011.jpg)
![Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NLS932] - Analysis of Human Brain. 8 ug/ ml.](http://images.novusbio.com/fullsize/Melatonin-Receptor-1B-Antibody-Immunohistochemistry-Paraffin-NLS932-img0006.jpg)
![Immunohistochemistry: Melatonin Receptor 1B Antibody [NLS932] - Analysis of Human Brain using Fast Red with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Melatonin-Receptor-1B-Antibody-Immunohistochemistry-NLS932-img0004.jpg)
![Immunohistochemistry: Melatonin Receptor 1B Antibody [NLS932] - Staining of Mouse Brain.](http://images.novusbio.com/fullsize/Melatonin-Receptor-1B-Antibody-Immunohistochemistry-NLS932-img0005.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NLS932] - Tissue localization of melatonin receptor type 1B (MTR1B) in the ventral prostate of rats. Short-term control treated with MLT (M1). Influence of Melatonin on the Proliferative and Apoptotic Responses of the Prostate under Normal and Hyperglycemic Conditions. <i>J Diabetes Res</i> (2015)](http://images.novusbio.com/fullsize/Melatonin-Receptor-1B-Antibody-Immunohistochemistry-Paraffin-NLS932-img0010.jpg)
![Immunohistochemistry: Melatonin Receptor 1B Antibody - BSA Free [NLS932] - Tissue localization of melatonin receptor type 1B (MTR1B) in the ventral prostate of rats. (a) Short-term control (C1); (b) short-term control treated with MLT (M1); (c) short-term untreated diabetic (D1); (d) short-term diabetic treated with MLT (MD1); (e) long-term control (C2); (f) long-term control treated with MLT (M2); (g) long-term untreated diabetic (D2); (h) long-term diabetic treated with MLT (MD2). (i) Negative control; (j) positive control, brain tissue section of rat. (k) Relative frequency (%) for MTR1B-positive areas. e: epithelium; l: lumen; s: stroma. Magnification: 400x, bar = 25 μm. Light bars: short-term experiment & dark bars: long-term experiment (N = 5 animals/group). Different lowercase letters indicate significant differences between experimental groups C1, M1, D1, & MD1 (nonparametric data), & different capital letters indicate significant differences between groups C2, M2, D2, & MD2 (parametric data), according to ANOVA followed by the Tukey (post hoc) or Kruskal-Wallis test followed by Dunn's test (post hoc). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26295055), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls932_rabbit-polyclonal-melatonin-receptor-1b-antibody-310202416114744.jpg)
![Immunohistochemistry: Melatonin Receptor 1B Antibody - BSA Free [NLS932] - Tissue localization of melatonin receptor type 1B (MTR1B) in the ventral prostate of rats. (a) Short-term control (C1); (b) short-term control treated with MLT (M1); (c) short-term untreated diabetic (D1); (d) short-term diabetic treated with MLT (MD1); (e) long-term control (C2); (f) long-term control treated with MLT (M2); (g) long-term untreated diabetic (D2); (h) long-term diabetic treated with MLT (MD2). (i) Negative control; (j) positive control, brain tissue section of rat. (k) Relative frequency (%) for MTR1B-positive areas. e: epithelium; l: lumen; s: stroma. Magnification: 400x, bar = 25 μm. Light bars: short-term experiment & dark bars: long-term experiment (N = 5 animals/group). Different lowercase letters indicate significant differences between experimental groups C1, M1, D1, & MD1 (nonparametric data), & different capital letters indicate significant differences between groups C2, M2, D2, & MD2 (parametric data), according to ANOVA followed by the Tukey (post hoc) or Kruskal-Wallis test followed by Dunn's test (post hoc). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26295055), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls932_rabbit-polyclonal-melatonin-receptor-1b-antibody-310202416114737.jpg)
![Immunohistochemistry: Melatonin Receptor 1B Antibody - BSA Free [NLS932] - Tissue localization of melatonin receptor type 1B (MTR1B) in the ventral prostate of rats. (a) Short-term control (C1); (b) short-term control treated with MLT (M1); (c) short-term untreated diabetic (D1); (d) short-term diabetic treated with MLT (MD1); (e) long-term control (C2); (f) long-term control treated with MLT (M2); (g) long-term untreated diabetic (D2); (h) long-term diabetic treated with MLT (MD2). (i) Negative control; (j) positive control, brain tissue section of rat. (k) Relative frequency (%) for MTR1B-positive areas. e: epithelium; l: lumen; s: stroma. Magnification: 400x, bar = 25 μm. Light bars: short-term experiment & dark bars: long-term experiment (N = 5 animals/group). Different lowercase letters indicate significant differences between experimental groups C1, M1, D1, & MD1 (nonparametric data), & different capital letters indicate significant differences between groups C2, M2, D2, & MD2 (parametric data), according to ANOVA followed by the Tukey (post hoc) or Kruskal-Wallis test followed by Dunn's test (post hoc). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26295055), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls932_rabbit-polyclonal-melatonin-receptor-1b-antibody-310202416121978.jpg)
![Western Blot: Melatonin Receptor 1B Antibody - BSA Free [NLS932] - Effects of luzindole on inhibitory action of melatonin on TGF-beta 1-stimulated proliferation & activation in renal interstitial fibroblasts. (A) Reverse transcription-polymerase chain reaction analysis of melatonin receptor type 1A (MT1) & type 1B (MT2) in NRK-49F cells. (B) NRK-49F cells were treated with with TGF-beta 1 (5 ng/mL) after preincubation with vehicle (Veh) or melatonin (Mel; 1mM) in the presence or absence of luzindole (Luz; 100 μM) for 30 min. Cell viability was assessed using CCK-8 assay at 0, 24, & 48 h. The OD was measured at 450 nm. (C) Western blot analysis for alpha -SMA & p-Smad2/3. Cells were treated with TGF-beta 1 (5 ng/mL) for 24 h after preincubation with Veh or Mel (1 mM) in the presence or absence of luzindole (Luz; 20 μM or 100 μM) for 30 min. The graphs show the results of quantitative analysis of alpha -SMA (D) & p-Smad2/3 (E). ** p < 0.01 & *** p < 0.001 vs. Veh-treated cells. #p < 0.05 vs. TGF-beta 1-treated cells. NS: not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906396), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls932_rabbit-polyclonal-melatonin-receptor-1b-antibody-3102024161367.jpg)
![Immunohistochemistry: Melatonin Receptor 1B Antibody - BSA Free [NLS932] - Tissue localization of melatonin receptor type 1B (MTR1B) in the ventral prostate of rats. (a) Short-term control (C1); (b) short-term control treated with MLT (M1); (c) short-term untreated diabetic (D1); (d) short-term diabetic treated with MLT (MD1); (e) long-term control (C2); (f) long-term control treated with MLT (M2); (g) long-term untreated diabetic (D2); (h) long-term diabetic treated with MLT (MD2). (i) Negative control; (j) positive control, brain tissue section of rat. (k) Relative frequency (%) for MTR1B-positive areas. e: epithelium; l: lumen; s: stroma. Magnification: 400x, bar = 25 μm. Light bars: short-term experiment & dark bars: long-term experiment (N = 5 animals/group). Different lowercase letters indicate significant differences between experimental groups C1, M1, D1, & MD1 (nonparametric data), & different capital letters indicate significant differences between groups C2, M2, D2, & MD2 (parametric data), according to ANOVA followed by the Tukey (post hoc) or Kruskal-Wallis test followed by Dunn's test (post hoc). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26295055), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls932_rabbit-polyclonal-melatonin-receptor-1b-antibody-310202416121951.jpg)
![Western Blot: CRHR2/CRF2 Antibody [NB100-56485] - Analysis of CRHR2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Both of the isoforms detected by this antibody.](http://images.novusbio.com/fullsize/CRHR2-CRF2-Antibody-Western-Blot-NB100-56485-img0002.jpg "Western Blot: CRHR2/CRF2 Antibody [NB100-56485] - Analysis of CRHR2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Both of the isoforms detected by this antibody.")
![Simple Western: CRHR2/CRF2 Antibody [NB100-56485] - Shows a specific band for Corticotropin Releasing Factor 2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CRHR2-CRF2-Antibody-Simple-Western-NB100-56485-img0003.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0012.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0011.jpg)
![Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Image shows a specific band for Calcium Sensing Receptor in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Simple-Western-NB100-1830-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241003248.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody (HL 1499) NB100-1830PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-1772024959417.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Analysis of FFPE human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxid](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241044639.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on HEK293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-177202495941.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720249362617.jpg)
![Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Total protein from Human and mouse Thymus, Jurkat and U2OS cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ROR Gamma in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ROR-gamma-RORC-NR1F3-Antibody-Western-Blot-NBP2-24503-img0007.jpg "Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Total protein from Human and mouse Thymus, Jurkat and U2OS cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ROR Gamma in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - U-2 OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ROR gamma overnight at 4C and detected with an anti-rabbit Dylight 488 (Green). Alpha tubulin (DM1A) NB100-690 was used as a co-stain and detected with an anti-mouse Dylight 550 (Red). Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ROR-gamma-RORC-NR1F3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24503-img0005.jpg)
![Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Tissue section of human lymph node using anti- ROR gamma/RORC/NR1F3 antibody. The staining was developed with HRP labeled secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This antibody generated mainly a nuclear staining in a subset of cells and a weak to cytoplasmic staining was also observed in some cells.](http://images.novusbio.com/fullsize/ROR-gamma-RORC-NR1F3-Antibody-Immunohistochemistry-Paraffin-NBP2-24503-img0006.jpg)
![Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Analysis of human ROR gamma/RORC/NR1F3 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide, 3) in 3T3, and 4) in RAW cell lysate using NBP2-24503.](http://images.novusbio.com/fullsize/ROR-gamma-RORC-NR1F3-Antibody-Western-Blot-NBP2-24503-img0004.jpg)
![Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Analysis of human testis using NBP2-24503.](http://images.novusbio.com/fullsize/ROR-gamma-RORC-NR1F3-Antibody-Immunohistochemistry-Paraffin-NBP2-24503-img0003.jpg)
![Western Blot: BAI1 Antibody [NB110-81586] - analysis of BAI1 in mouse brain tissue using anti-BAI1 antibody. KO mice was used as negative controls (right lane). Image from verified customer review.](http://images.novusbio.com/fullsize/BAI1-Antibody-Western-Blot-NB110-81586-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BAI1 Antibody [NB110-81586] - analysis of BAI1 in mouse brain tissue using anti-BAI1 antibody. KO mice was used as negative controls (right lane). Image from verified customer review.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BAI1 Antibody [NB110-81586] - BAI1 antibody was tested in SH-SY-5Y cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/BAI1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-81586-img0001.jpg)
![Immunohistochemistry-Paraffin: BAI1 Antibody [NB110-81586] - A. Staining of mouse brain using NB110-81586. B. Blocking of staining using control peptide, NB110-81586PEP.](http://images.novusbio.com/fullsize/BAI1-Antibody-Immunohistochemistry-Paraffin-NB110-81586-img0003.jpg)