Adenosine A2aR Antibody (7F6-G5-A2) [PE/Cy5.5]

• Reactivity: Hu, Mu, Rt, Po, Ca, Gp, Rb, Sh
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 7F6-G5-A2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE/Cy5.5

Adenosine A2aR Antibody (7F6-G5-A2) [PE/Cy5.5] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Full length recombinant human Adenosine A2a R. [UniProt# P29274]
• Epitope: Epitope mapped to SQPLPGER in the third intracellular loop (PMID 9822147).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADORA2A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 29791204). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Cy is a registered trademark of Global Life Sciences Solutions Germany GmbH.

Alternate Names for Adenosine A2aR Antibody (7F6-G5-A2) [PE/Cy5.5]

• A2aR
• adenosine A2 receptor
• Adenosine A2a R
• adenosine A2a receptor
• Adenosine A2aR
• adenosine receptor A2a
• adenosine receptor subtype A2a
• ADORA 2
• ADORA2
• ADORA2A
• hA2aR
• RDC8

Background

Adenosine A2a Receptor encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADORA2A