GLP-1R Antibody

Images

 
Western Blot: GLP-1R Antibody [NLS1205] - WB detection of GLP-1R in lysates of human (1) pancreas (2) lung and (3) kidney tissues using 2 ug/ml concentration of GLP-1R antibody.
Immunohistochemistry: GLP-1R Antibody [NLS1205] - IHC analysis of GLP-1R in mouse pancreas.
Immunohistochemistry: GLP-1R Antibody [NLS1205] - human pancreas (Islets of Langerhans).
Simple Western: GLP-1R Antibody [NLS1205] - Simple Western lane view shows a specific band for GLP-1R in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

GLP-1R Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal extracellular portion of the human GLP1R protein (between residues 100-200) [UniProt P43220]
Localization
Cell membrane
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLP1R
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Simple Western 1:50
  • Immunohistochemistry 10-20 ug/ml
  • Immunohistochemistry-Paraffin 10-20 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Pancreas Lysate (NB820-59244)
Control Peptide
GLP-1R Peptide (NLS1205PEP)
Publications
Read Publications using
NLS1205 in the following applications:

Reactivity Notes

Human and mouse. The immunogen used for this antibody has 100/88% homology/identity with the rat protein.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GLP-1R Antibody

  • GLP-1 receptor
  • GLP1R
  • GLP-1R
  • GLP-1-R
  • glucagon-like peptide 1 receptor
  • MGC138331

Background

GLP1R, a Glucagon Receptor, has been suggested to affect the feelings of satiety or hunger, sensation of glucose levels, control of glucagon sensitivity of islets, and non insulin-dependent diabetes mellitus. Glucagon-like peptide 1 is an incretin hormone produced by enteroendocrine L-cells in the intestinal mucosa. The hormone is released in response to food intake and plays an important role in maintaining blood glucose homeostasis. Stimulation of the GLP-1R by endogenous hormone induces multiple complementary mechanisms, which together result in a lowering of circulating blood glucose levels. These mechanisms include receptor-mediated enhancement of glucose-induced insulin secretion from pancreatic -cells, inhibition of gastric emptying with a delay in the gastrointestinal resorption of nutrients, inhibition of glucagon secretion, and inhibition of food intake. Desensitization of GLP1R on pancreatic beta-cells is one of the causes of non insulin-dependent diabetes mellitus (NIDDM). GLP1R knockout mice are viable, develop normally, but exhibit increased levels of blood glucose following oral glucose challenge in association with diminished levels of circulating insulin. Recently it has been shown that overexpression of glucagon-like peptide-1 receptor improves learning in rats (During et al. 2003). GLP1R has been reported in pancreas, brain, heart, kidney, lung, and stomach. ESTs have been isolated from kidney and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GLP-1R Antibody (NLS1205)(4)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
All Applications
Filter By Species
Mouse
(1)
Rat
(2)
All Species
Showing Publications 1 - 4 of 4.
Publications using NLS1205 Applications Species
Kimura T, Obata A, Shimoda M et al. Decreased glucagon-like peptide 1 receptor expression in endothelial and smooth muscle cells in diabetic db/db mice: TCF7L2 is a possible regulator of the vascular glucagon-like peptide 1 receptor Diab Vasc Dis Res 2017 Aug 01 [PMID: 28830217] (Mouse) Mouse
Karabulut S, Coskun ZM, Bolkent S. Immunohistochemical, apoptotic and biochemical changes by dipeptidyl peptidase-4 inhibitor-sitagliptin in type-2 diabetic rats. Pharmacol Rep 2015 Oct [PMID: 26398375] (Rat) Rat
Matveyenko AV, Dry S, Cox HI et al. Beneficial Endocrine but Adverse Exocrine Effects of Sitagliptin in the Human Islet Amyloid Polypeptide Transgenic Rat Model of Type 2 Diabetes: Interactions With Metformin. Diabetes;58(7):1604-1615. 2009 [PMID: 19403868]
Karabulut S, Coskun ZM, Bolkent S. Immunohistochemical, apoptotic and biochemical changes by dipeptidyl peptidase-4 inhibitor-sitagliptin in type-2 diabetic rats Pharmacological Reports. 2015 Feb 01 (IHC-P, Rat)

Details:
GLP-1R antibody used at 1:50 dilution for IHC-P application on formalin fixed paraffin embedded (10% formalin-PBS for 24 hours at 4C) pancreatic tissue samples from Rats which were subjected or not to streptozotocin /STZ-induced diabetes and/or treatment with dipeptidyl peptidase-4 inhibitor-sitagliptin. Signal detection was carried out using streptavidin-biotin-peroxidase method with histostain-plus bulk kit and 3-amino-9-ethyl-carbazole/AEC substrate kit (Fig 5).
IHC-P Rat

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GLP1R
Entrez
Uniprot