GLP-1R Antibody

Images

 
Biological Strategies: Simple Western: GLP-1R Antibody [NLS1205] - Capillary western blot and fold change in the expression of GLP-1 receptors (GLP-1R) in the rat retina. *P < .05, significantly different from ...read more
Immunocytochemistry: GLP-1R Antibody [NLS1205] - Expression of GLP-1 receptors in human retinal microvascular endothelial cells (HRMECs). (i) Endothelial cells labelled with endothelin-1 (ET-1) antibodies (green). (j) ...read more
Western Blot: GLP-1R Antibody [NLS1205] - WB detection of GLP-1R in lysates of human (1) pancreas (2) lung and (3) kidney tissues using 2 ug/mL concentration of GLP-1R antibody.
Immunohistochemistry: GLP-1R Antibody [NLS1205] - Analysis of GLP-1R in mouse pancreas.
Immunohistochemistry: GLP-1R Antibody [NLS1205] - Human pancreas (Islets of Langerhans).
Simple Western: GLP-1R Antibody [NLS1205] - Image shows a specific band for GLP-1R in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Microarray: GLP-1R Antibody [NLS1205] - Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. Microarray image submitted by a verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, MiAr, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

GLP-1R Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal extracellular portion of the human GLP1R protein (between residues 100-200). [UniProt P43220]
Localization
Cell membrane
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLP1R
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 10 - 20 ug/mL
  • Immunohistochemistry-Paraffin 10 - 20 ug/mL
  • Microarray
  • Simple Western 1:50
  • Western Blot 1 - 2 ug/mL
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32232832). This GLP-1R Antibody is validated for microarray from a verified customer review.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Pancreas Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
GLP-1R Peptide (NLS1205PEP)
Reviewed Applications
Read 1 Review rated 4
using
NLS1205 in the following applications:

Publications
Read Publications using
NLS1205 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 26398375).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GLP-1R Antibody

  • GLP-1 receptor
  • GLP1R
  • GLP-1R
  • GLP-1-R
  • glucagon-like peptide 1 receptor
  • MGC138331

Background

GLP1R, a Glucagon Receptor, has been suggested to affect the feelings of satiety or hunger, sensation of glucose levels, control of glucagon sensitivity of islets, and non insulin-dependent diabetes mellitus. Glucagon-like peptide 1 is an incretin hormone produced by enteroendocrine L-cells in the intestinal mucosa. The hormone is released in response to food intake and plays an important role in maintaining blood glucose homeostasis. Stimulation of the GLP-1R by endogenous hormone induces multiple complementary mechanisms, which together result in a lowering of circulating blood glucose levels. These mechanisms include receptor-mediated enhancement of glucose-induced insulin secretion from pancreatic -cells, inhibition of gastric emptying with a delay in the gastrointestinal resorption of nutrients, inhibition of glucagon secretion, and inhibition of food intake. Desensitization of GLP1R on pancreatic beta-cells is one of the causes of non insulin-dependent diabetes mellitus (NIDDM). GLP1R knockout mice are viable, develop normally, but exhibit increased levels of blood glucose following oral glucose challenge in association with diminished levels of circulating insulin. Recently it has been shown that overexpression of glucagon-like peptide-1 receptor improves learning in rats (During et al. 2003). GLP1R has been reported in pancreas, brain, heart, kidney, lung, and stomach. ESTs have been isolated from kidney and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, MiAr, ICC/IF

Publications for GLP-1R Antibody (NLS1205)(4)

Review for GLP-1R Antibody (NLS1205) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NLS1205:
Filter by Applications
MiAr
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Microarray GLP-1R NLS1205
Enlarge
4
reviewed by:
Verified Customer
MiAr Human 12/07/2020
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Summary

ApplicationMicroarray
Sample TestedEDTA Plasma
SpeciesHuman
LotA-7

Product General Protocols

View specific protocols for GLP-1R Antibody (NLS1205): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GLP-1R Antibody (NLS1205) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NLS1205AF405
Alexa Fluor 488 NLS1205AF488
Alexa Fluor 647 NLS1205AF647
Alexa Fluor 700 NLS1205AF700
Biotin NLS1205B
DyLight 350 NLS1205UV
DyLight 405 NLS1205V
DyLight 488 NLS1205G
DyLight 550 NLS1205R
DyLight 650 NLS1205C
DyLight 680 NLS1205FR
DyLight 755 NLS1205IR
FITC NLS1205F
HRP NLS1205H

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
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Verified Customer
12/07/2020
Application: MiAr
Species: Human

Bioinformatics

Gene Symbol GLP1R
Entrez
Uniprot