Western Blot: GLP-1R Antibody [NLS1205] - WB detection of GLP-1R in lysates of human (1) pancreas (2) lung and (3) kidney tissues using 2 ug/mL concentration of GLP-1R antibody.
Immunohistochemistry: GLP-1R Antibody [NLS1205] - Analysis of GLP-1R in mouse pancreas.
Immunohistochemistry: GLP-1R Antibody [NLS1205] - Human pancreas (Islets of Langerhans).
Simple Western: GLP-1R Antibody [NLS1205] - Image shows a specific band for GLP-1R in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
53 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for GLP-1R Antibody
glucagon-like peptide 1 receptor
GLP1R, a Glucagon Receptor, has been suggested to affect the feelings of satiety or hunger, sensation of glucose levels, control of glucagon sensitivity of islets, and non insulin-dependent diabetes mellitus. Glucagon-like peptide 1 is an incretin hormone produced by enteroendocrine L-cells in the intestinal mucosa. The hormone is released in response to food intake and plays an important role in maintaining blood glucose homeostasis. Stimulation of the GLP-1R by endogenous hormone induces multiple complementary mechanisms, which together result in a lowering of circulating blood glucose levels. These mechanisms include receptor-mediated enhancement of glucose-induced insulin secretion from pancreatic -cells, inhibition of gastric emptying with a delay in the gastrointestinal resorption of nutrients, inhibition of glucagon secretion, and inhibition of food intake. Desensitization of GLP1R on pancreatic beta-cells is one of the causes of non insulin-dependent diabetes mellitus (NIDDM). GLP1R knockout mice are viable, develop normally, but exhibit increased levels of blood glucose following oral glucose challenge in association with diminished levels of circulating insulin. Recently it has been shown that overexpression of glucagon-like peptide-1 receptor improves learning in rats (During et al. 2003). GLP1R has been reported in pancreas, brain, heart, kidney, lung, and stomach. ESTs have been isolated from kidney and skin libraries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Karabulut S, Coskun ZM, Bolkent S. Immunohistochemical, apoptotic and biochemical changes by dipeptidyl peptidase-4 inhibitor-sitagliptin in type-2 diabetic rats Pharmacological Reports. 2015 Feb 01 (IHC-P, Rat)
Details: GLP-1R antibody used at 1:50 dilution for IHC-P application on formalin fixed paraffin embedded (10% formalin-PBS for 24 hours at 4C) pancreatic tissue samples from Rats which were subjected or not to streptozotocin /STZ-induced diabetes and/or treatment with dipeptidyl peptidase-4 inhibitor-sitagliptin. Signal detection was carried out using streptavidin-biotin-peroxidase method with histostain-plus bulk kit and 3-amino-9-ethyl-carbazole/AEC substrate kit (Fig 5).
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