Adenosine A2aR Antibody (7F6-G5-A2) [FITC]

Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [FITC] [NBP1-39474F] - An intracellular stain was performed on U-87 MG cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [FITC] [NBP1-39474F] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

• Reactivity: Hu, Mu, Rt, Po, Ca, Gp, Rb, Sh
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 7F6-G5-A2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

Adenosine A2aR Antibody (7F6-G5-A2) [FITC] Summary

• Immunogen: Full length recombinant human Adenosine A2a R. [UniProt# P29274]
• Epitope: Epitope mapped to SQPLPGER in the third intracellular loop (PMID 9822147).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADORA2A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 29791204). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Adenosine A2aR Antibody (7F6-G5-A2) [FITC]

• A2aR
• adenosine A2 receptor
• Adenosine A2a R
• adenosine A2a receptor
• Adenosine A2aR
• adenosine receptor A2a
• adenosine receptor subtype A2a
• ADORA 2
• ADORA2
• ADORA2A
• hA2aR
• RDC8

Background

Adenosine A2a Receptor encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Adenosine A2aR Antibody (NBP1-39474F) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 12/19/2017

Summary

• Application: Flow Cytometry
• Sample Tested: Colon cancer cell line
• Species: Mouse
• Lot: A-7-082217

Bioinformatics

• Gene Symbol: ADORA2A