Core ESC-Like Genes reagents: Novus Biologicals

NB100-56519

DNMT1 Antibody (60B1220.1) - BSA Free

(4 Reviews)

(144 Publications)

0.1 mg

NB100-56519

Immunohistochemistry-Paraffin: DNMT1 Antibody (60B1220.1) [NB100-56519] - Tissue section of human hepatocellular carcinoma at 1:50 dilution.

Flow Cytometry: DNMT1 Antibody (60B1220.1) [NB100-56519] - An intracellular stain was performed on HeLa cells with DNMT1 [60B1220.1] Antibody NB100-56519AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of PE conjugate of NB100-56519. An intracellular stain was performed on HeLa cells with DNMT1 antibody (60B1220.1) NB100-56519PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0

Simple Western: DNMT1 Antibody (60B1220.1) [NB100-56519] - Simple Western lane view shows a specific band for Dnmt1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunoprecipitation: DNMT1 Antibody (60B1220.1) [NB100-56519] - The PCNAS228I mutation differentially affects PCNA binding to PIP box-containing proteins. GST-PIP pull down of His-S-PCNAWT or PCNAS228I. Figure shows Coomassie stained gel of representative pull down (top) and anti-PCNA western blot of the same samples diluted 1:20 (bottom). Amount of 'input' loaded for Coomassie is equivalent to 1%, 'Glut. beads' (Glutathione sepharose 4B beads) is equivalent to 25%. Molecular weight markers are indicated. Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S1568786416304013), licensed under a CC-BY license.

Western Blot: DNMT1 Antibody (60B1220.1) - BSA Free [NB100-56519] - WA does not alter DNMTs gene & protein expression(A) Effect of WA on DNMT1, DNMT3A & DNMT3B gene expression in MDA-MB-231 & MCF-7 cells normalized to select housekeeping genes & relative to DMSO-treated MCF-7 sample (2-delta delta Ct) as determined by Human Epigenetic Chromatin Modification Enzyme qPCR Array as previously described. Bars represent DNMT1, DNMT3A & DNMT3B relative mRNA (mean ± SEM) expression of at least two independent experiments. (B) Effect of WA on DNMT1, DNMT3A & DNMT3B protein expression in MDA-MB-231 & MCF-7 cells, as compared to to beta -Actin protein levels. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28467815), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Chromatin Immunoprecipitation: DNMT1 Antibody (60B1220.1) - BSA Free [NB100-56519] - Epigenetic changes & protein binding at Ankrd26 promoter in eAT from mice upon 22 weeks of HFD.ChIP of DNMT1, DNMT3a, DNMT3b (a) & MBD2 (b) binding at Ankrd26 promoter region (−553 bp/−348 bp). (c) MNase for Nuc-2 (−257 bp/−198 bp) & Nuc-1 (−84 bp/−25 bp) occupancy at Ankrd26 promoter. (d) ChIP for acetyl-H4 enrichment at Nuc-2 & Nuc-1. (e) ChIP of RNA Pol II binding at Ankrd26 TSS (+16 bp/+159 bp). (a,b) & (d,e), ChIP enrichment is relative to Input chromatin. (a–e), results are mean ± SD from three independent experiments. **p < 0.01 & ***p < 0.001 vs STD. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep43526), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: DNMT1 Antibody (60B1220.1) - BSA Free [NB100-56519] - Verification of the expression of the proteins identified.(A) Validation of the SILAC results of selected proteins in immunoblots of protein extracts from the bladder cancer cells analyzed. The results validated the expression levels of proteins identified by the proteomic approach, including differentially & non-differentially expressed candidates. Antibodies displaying a single predominant band at the expected molecular weights were accepted: & alpha -tubulin, was used as the loading control. GSN, Gelsolin; Cul3, Cullin 3; IPO9. Importin 9; EGFR, Epidermal Growth Factor Receptor; NUP133, Nucleoporin 133; HSP70, Heat Shock Protein 70kDa; MCM6, Minichromosome Maintenance Complex Component 6; RCC1, Regulator of Chromosome Condensation 1; BCAS2, Breast Carcinoma Amplified Sequence 2; DNM, Dynamin; NPM, Nucleophosmin; DCTN, Dynactin; CALR, Calreticulin; MAPK, Mitogen-Activated Protein Kinase; DDX21, DEAD (Asp-Glu-Ala-Asp) box polypeptide 21; CDC2: Cell Division Cycle 2; DNMT1, DNA (cytosine-5)-Methyltransferase 1; MSH6, MutS Homolog 6; RAB14, GTPase Rab14; VDAC, Voltage-Dependent Anion Channel; CK18, Cytokeratin 18; CALD, Caldesmon; CD44, CD44 antigen isoform 1 precursor 2; EZR, Ezrin; MSN, Moesin; ANXA2, Annexin A2; CPNE3, Copine 3; FLNA, Filamin A; CAV1, Caveolin 1. Western Blots were scanned & analyzed using alpha -tubulin as normalizing control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23308193), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Sh

Applications:

, , , , , , , , ,

Applications: , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-261A

Alt Catalog# IMG-261A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-56519AF350
Alexa Fluor 405	NB100-56519AF405
Alexa Fluor 488	NB100-56519AF488
Alexa Fluor 532	NB100-56519AF532
Alexa Fluor 594	NB100-56519AF594
Alexa Fluor 647	NB100-56519AF647
Alexa Fluor 700	NB100-56519AF700
Alexa Fluor 750	NB100-56519AF750
Allophycocyanin	NB100-56519APC
Biotin	NB100-56519B
CoraFluor 1	NB100-56519CL1
DyLight 350	NB100-56519UV
DyLight 405	NB100-56519V
DyLight 488	NB100-56519G
DyLight 550	NB100-56519R
DyLight 594	NB100-56519DL594
DyLight 650	NB100-56519C
DyLight 680	NB100-56519FR
DyLight 755	NB100-56519IR
FITC	NB100-56519F
HRP	NB100-56519H
Janelia Fluor 525	NB100-56519JF525
Janelia Fluor 549	NB100-56519JF549
Janelia Fluor 646	NB100-56519JF646
Janelia Fluor 669	NB100-56519JF669
PE	NB100-56519PE
mFluor Violet 450 SE	NB100-56519MFV450
mFluor Violet 500 SE	NB100-56519MFV500
mFluor Violet 610 SE	NB100-56519MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56519SS

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NB100-116

APE Antibody (13B8E5C2) - BSA Free

(3 Reviews)

(108 Publications)

0.1 mg

NB100-116

Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - HeLa cells stained NB100-116 (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).

Immunohistochemistry: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Immunohistochemical staining of APE1. A. Nuclear staining in the luminal epithelium of normal breast ducts and lobules. B. Low, and C. high nuclear APE1 expression in invasive breast cancer. (magnification x400). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0099528) licensed under a CC-BY license.

<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation (ChIP): APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Association of p53 and APE1 on p53-binding sites in p21 promoter. Re-ChIP analysis (first IP with alpha-APE1 and the second IP with alpha-p53 antibody) showing simultaneous recruitment of APE1 and p53 in control vs. EPE treated cells; *: p value <0.05 (n=2) calculated based on APE1/p53 enriched DNA from control vs. etoposide treated cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY license.

Simple Western: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Image shows a specific band for APE1 in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-APE (13B8E5C2) at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Ovarian Cancer cell lines.

Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Immunocytochemical detection of APE-ref-1 in breast cancer cell line MDA MB 231.

Immunohistochemistry-Paraffin: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - APE1 antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.

Chromatin Immunoprecipitation: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - Association of APE1 & AP4 in p21 proximal promoter region.(A & B) ChIP Real Time PCR analysis showing relative enrichment of (A) APE1 & (B) AP4 in p21 proximal promoter containing AP4-responsive E-Box elements in HCT116p53null cells. (C) Re-ChIP (first IP with alpha -APE1 & the second IP with alpha -AP4 antibody) analysis showing simultaneous recruitment of APE1 & AP4 in this promoter region. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23874636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - Effect of APE1 on p21 activation in p53 WT cells.(A) Western analysis for APE1 level in WT APE1 & N delta 42 APE1 overexpressing cells. (B) Real Time RT-PCR analysis showing relative quantitation of p21 transcript level in etoposide-treated WT APE1-overexpressing HCT116WT cells & N delta 42 APE1 overexpressing cells; *: p value <0.05 (n = 2) calculated from control (empty vector transfection) vs. WT APE1 overexpression, WT APE1 overexpression along with etoposide treatment or N delta 42 overexpression. (C) Luciferase activity in cells co-transfected with empty or WT APE1-expression vector & p21 promoter-luciferase construct; *: p value <0.05 (n = 2) calculated from control (empty vector transfection) vs. APE1 overexpression. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23874636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - Analysis of single strand break repair in MCF-7 monolayer & mammosphere cell populations. (a) Cells were exposed to 4 Gy 60Co gamma -radiation & the relative degree of single-strand breakage (SSB) was determined by alkaline single-cell gel electrophoresis (comet assay) immediately after exposure & at the times indicated after exposure. (b) The 'comets' (n of about 100) were categorized according to the NIH LISTSERV (Comet Assay Interest Group web site) in which type 1 comets display the least DNA damage & type 5 the most. The error bars represent the mean ± standard error of the mean in both panels. The comets of the unirradiated cells are labeled Cont. (c) Expression of proteins involved in SSB repair in response to ionizing radiation. Lysates were prepared from unirradiated cells & from cells harvested one hour after exposure to 1 or 10-Gy 60Co gamma -radiation & analyzed by immunoblotting with antibodies against several SSB repair proteins. alpha -Actin served as a loading control. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr2583), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - Intracellular APE1/Ref-1 was targeted to the plasma membrane in response to acetylation. (A & B) Whole cell lysates were immunoprecipitated using an anti-acetyl lysine antibody, followed by immunoblotting with the polyclonal anti-APE1/Ref-1 antibody. The blots were stripped & re-probed with anti-beta -actin & APE1/Ref-1 antibody to ensure equal protein loading. Similar results were obtained from replicate experiments. Column, mean (n = 3); bars, SE. *, p < 0.05, significantly different compared with control or between group by one-way ANOVA followed by Bonferroni’s multiple comparison test. (C,D) Membrane fractions or whole cell lysates were prepared from the TSA-treated cells. Immunoblotting for APE1/Ref-1 was performed using the polyclonal anti-APE1/Ref-1 antibody. Blots were stripped & re-probed with anti-N-cadherin & anti-beta -actin antibodies to control for differences in protein loading. Fold changes in the levels of APE1/Ref-1 in the plasma membrane fraction relative to the control are shown. †, indicates molecular marker (N-cadherin) of left image. Column mean (n = 3); bars, SE.*, p < 0.05 indicates a significantly different result compared with control or between groups by one-way ANOVA followed by Bonferroni’s multiple comparison test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31261750), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - AcAPE1 is exclusively associated w/ chromatin & remains bound to condensed chromosomes. (A & B) Asynchronous normal lung fibroblast IMR90 cells & lung adenocarcinoma A549 cells immunostained w/ anti-APE1 & anti-AcAPE1 Abs, counterstained w/ DAPI, & visualized by confocal microscopy & 3D SIM. (C) Colocalization of AcAPE1 w/ histone H3 or active enhancer-specific histone marker acetylated H3K27 (H3K27Ac). (D) BJ-hTERT cells serum starved for 72 h & then fixed at different time points. Cells immunostained w/ anti-APE1 & anti-AcAPE1 Abs & counterstained w/ anti-TO-PRO-3 iodide Ab. (E) Mitotic A549 cells immunostained w/ anti-APE1 & anti-AcAPE1 & visualized by 3D SIM. (F) BJ-hTERT cells either serum starved for 72 h (G0/G1 phase), treated w/ nocodazole (mitotic cells) or aphidicolin (G1/S phase synchronized cells), or untreated, & whole-cell extracts isolated using 150 mM or 300 mM salt-containing lysis buffer. Western blot analysis for anti-APE1 & anti-AcAPE1 levels performed. Anti-HSC70 used as loading control. (G) A proximal ligation assay performed w/ mouse anti-APE1 & rabbit anti-APE1 (mAPE1 & Rabbit-APE1), mouse anti-mouse APE1 & rabbit anti-AcAPE1 (mAPE1 & rAcAPE1), & rabbit anti-AcAPE1 & mouse anti-histone H3 (mHistone H3 & rAcAPE1) to confirm the chromatin association of AcAPE1. Mouse IgG (mIgG) & rabbit anti-AcAPE1 used as a control. At least 50 cells counted for PLA foci. (H) Colocalization of p300 & AcAPE1 on chromatin (DAPI). (I) HCT116 cells transfected w/ E1A & mutant E1A, & at 48 h after transfection, IF performed. Cells immunostained w/ anti-p300 & anti-APE1 or anti-AcAPE1 & counterstained w/ DAPI. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27994014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - XPF is epistatic to APE in the gemcitabine resistance. The expression of XPF and/or APE was suppressed by siRNAs in HeLa cells & the cellular sensitivity to gemcitabine was examined. XPF- or APE-suppressed HeLa cells (closed square & closed triangle, respectively) showed sensitivity to gemcitabine. Cosuppression of XPF & APE (closed circle with dashed line) resulted in the sensitivity to gemcitabine similar to the sensitivity induced by the suppression of XPF or APE individually. A control siRNA (siControl) was used as a control (open diamond). Three independent experiments were performed & averages of surviving fraction are plotted. The error bars show standard deviations. The differences in the gemcitabine sensitivity between the control cells & the cells treated with siXPF, siAPE, or siXPF+siAPE are statistically significant at 10 nM & 50 nM with p<0.05. The western blots showed a significant suppression of XPF (more than 95%) & ~75% reduction in the expression of APE with the siRNA treatment. The cosuppression experiments with two siRNAs, siXPF & siAPE, resulted in similar levels of suppression of each protein induced by individual siRNA (more than 95% reduction in XPF & ~85% reduction in APE). Tubulin was used as a protein loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30941207), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - Plasma membrane associated APE1/Ref-1 is bound to ABCA1 in response to acetylation. Cells transiently expressing wild type APE1/Ref-1-FLAG or mutant APE1/Ref-1(K6/7R)-FLAG were treated with 1 µM TSA for 1 h. (A) Whole cell lysates were immunoprecipitated using the monoclonal anti-ABCA1 antibody, followed by immunoblot with the anti-FLAG antibody. (B) For reverse immunoprecipitation, cell lysates were immunoprecipitated with anti-APE1/Ref-1 antibody followed by immunoblot analysis with the polyclonal anti-ABCA1 antibody. Blots were stripped & re-probed with anti-ABCA1 or FLAG antibodies to ensure equal protein loading & no contamination of cellular proteins. Similar results were observed in replicate experiments. Columns, mean (n = 2-3); bars, SE. *, p < 0.05 indicates a significantly different result from control cells according to unpaired t-tests. (C) The binding between APE1/Ref-1 & ABCA1 in the plasma membrane was visualized using with a Duolink II PLA system with primary polyclonal anti-APE1/Ref-1 & monoclonal anti-ABCA1 antibodies (PLA†). The PLA-specific fluorescence which represents the APE1/Ref-1-ABCA1 signal, & the DAPI nuclear staining are in red & blue, respectively. The experiment was repeated multiple times with similar results; the data shown here are from a representative experiment. Optical slices were examined using a 40× oil immersion objective with a 2× zoom factor. Scale bar, 20 µm (×80). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31261750), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE1 acetylation enhances its stability on chromatin & its interaction with downstream BER proteins. (A) Colocalization of ligase III & AcAPE1 in A549 cells. Cells were immunostained with anti-ligase III & anti-AcAPE1 Abs. (B) WT or K5R mutant APE1-overexpressing HEK293T cells were treated with TSA-nicotinamide (NAM) for 6 h or not treated, & the nuclear extract was immunoprecipitated using anti-FLAG Ab & immunoblotted with anti-XRCC1 & anti-FLAG Abs. (C) A549 cells were fixed with paraformaldehyde before (top) or after treatment with Triton X-100 (0.5%) (middle) or Triton X-100 plus salt (100 mM KCl) (bottom) & immunostained with anti-APE1 or anti-AcAPE1 Abs & counterstained with DAPI. (D) Acetylation of APE1 induces a conformational change in APE1. The distinct intrinsic fluorescence emission spectra of APE1 & AcAPE1 at 280 nm are shown. A.U., absorbance units. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27994014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE1 is acetylated after binding to AP sites in the chromatin. (A) BJ-hTERT cells were treated with MX (50 mM) for the indicated times. IF was performed using anti-APE1 & anti-AcAPE1, & counterstaining with DAPI was used. (B) HCT116 cells were treated with various doses of MX for 30 min, IF was performed using anti-APE1 & anti-AcAPE1, & counterstaining with DAPI was used. (C) HCT116 cells were treated with 50 mM MX for 30 min, IF was performed using anti-OGG1, & counterstaining with DAPI was used. (D) BJ-hTERT cells pretreated with 50 mM MX for 30 min or not pretreated were exposed to MMS (2 mM) for 1 h. IF was performed using anti-APE1 & anti-AcAPE1, & counterstaining with DAPI was used. Confocal microscopy was used to visualize the AcAPE1 levels in control cells & cells treated with MMS or MX, or both. (E) ChIP with anti-OGG1 antibody followed by Western blotting (ChIP-on-Western) was performed to examine the association of AcAPE1 & ligase III on chromatin after induction of DNA damage with GO. (F) The association of AcAPE1 with the endogenous p21 promoter in control or MMS- or MX-treated cells was examined by promoter-directed ChIP using anti-AcAPE1. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27994014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - Repression of p21 by APE1 in p53-null cells & effect of ectopic p53 in this repression.(A & B) Real Time RT-PCR analysis showing relative quantitation of p21 transcript level in (A) HCT116p53null cells with WT & N delta 42 APE1 overexpression; *: p value (n = 4) calculated from control (empty vector transfection) vs. WT or N delta 42 APE1 overexpression, & (B) control (control siRNA) vs. APE1-depleted HCT116p53null cells; *: p value <0.05 (n = 4) calculated from control vs. APE1-depleted cells. (C) Effect of ectopic p53 expression on p21 transcript level in control vs. APE1-depleted HCT116p53null cells. First, cells were transfected with control siRNA or APE1 siRNA, the next day both the cell types were again transfected with empty vector or p53 expression vector & after 48 hrs the cells were harvested; signal from empty vector transfection in both control & APE1-depleted cells were set as reference samples; *: p value <0.05 (n = 3) calculated based on the effect of ectopic p53 expression over empty vector transfection in control vs. APE1-depleted cells. (D) Effect of APE1 depletion in control (empty vector transfected) vs. ectopic p53-expressing HCT116p53null cells; the same experiment was performed as in C but analyzed differently; signal from control siRNA-transfected cells in both empty vector transfected & ectopic p53 expressing cases were set as reference samples; *: p value <0.05 (n = 3) calculated based on the effect of APE1-depletion in empty vector transfected vs. ectopic p53 expressing cells. (E) Representative Western analysis of p53, APE1, p21 & alpha -Tubulin levels in the same HCT116p53null cells as in B–D. (F & G) Real Time RT-PCR analysis of p21 level in Saos2 cells as in C & D. *: p value <0.05 (n = 2). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23874636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - Intracellular APE1/Ref-1 was targeted to the plasma membrane in response to acetylation. (A & B) Whole cell lysates were immunoprecipitated using an anti-acetyl lysine antibody, followed by immunoblotting with the polyclonal anti-APE1/Ref-1 antibody. The blots were stripped & re-probed with anti-beta -actin & APE1/Ref-1 antibody to ensure equal protein loading. Similar results were obtained from replicate experiments. Column, mean (n = 3); bars, SE. *, p < 0.05, significantly different compared with control or between group by one-way ANOVA followed by Bonferroni’s multiple comparison test. (C,D) Membrane fractions or whole cell lysates were prepared from the TSA-treated cells. Immunoblotting for APE1/Ref-1 was performed using the polyclonal anti-APE1/Ref-1 antibody. Blots were stripped & re-probed with anti-N-cadherin & anti-beta -actin antibodies to control for differences in protein loading. Fold changes in the levels of APE1/Ref-1 in the plasma membrane fraction relative to the control are shown. †, indicates molecular marker (N-cadherin) of left image. Column mean (n = 3); bars, SE.*, p < 0.05 indicates a significantly different result compared with control or between groups by one-way ANOVA followed by Bonferroni’s multiple comparison test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31261750), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Chromatin Immunoprecipitation: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - Association of p53 & APE1 on p53-binding sites in p21 promoter.(A) p21 promoter structure showing p53 & AP4 binding sites. (B) ChIP Real Time PCR analysis showing relative enrichment (2− delta CT) of APE1-immunoprecipitated DNA over that from control IgG in p21 promoter regions containing p53 binding sites 1 & 2 in HCT116WT cells. (C) Re-ChIP analysis (first IP with alpha -APE1 & the second IP with alpha -p53 antibody) showing simultaneous recruitment of APE1 & p53 in control vs. etoposide treated cells; *: p value <0.05 (n = 2) calculated based on APE1/p53 enriched DNA from control vs. etoposide treated cells. (D) Western analysis of FLAG immunoprecipitate (IP) to detect APE1-associated p53 & FLAG (APE1) from empty vector vs. FLAG-tagged WT APE1 or FLAG-tagged N delta 33 APE1 transfected HCT116WT cells (left panel) & from control vs. etoposide-treated WT APE1-FLAG transfected cells (right panel). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23874636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - Gemcitabine-induced recruitment of XPF to chromatin depends on APE. HCT116, APE-suppressed HCT116 (HCT116 shAPE), & XPF-deficient HCT116 (HCT116 g4-10) cells were treated with 1 μM of gemcitabine & chromatin fractions were isolated in the indicated time. The presence of XPF & APE was detected by western blots. The asterisk (∗) shows a cross-reacted protein with the anti-XPF antibody. Histone H2AX was used as a loading control. Gemcitabine-induced recruitment of XPF to chromatin (lanes 1-3) was compromised in HCT116 shAPE cell line (lanes 4-5). The chromatin-bound APE is not changed by the gemcitabine treatment (lanes 4-6, Supplementary Figure 5). A signal of XPF (and APE) was normalized with a signal of H2AX in each chromatin fraction using ImageJ software. Then a ratio of chromatin-bound XPF with gemcitabine to XPF in control was determined & depicted as bar graphs. Three independent experiments were performed & averages of the ratio at indicated time points were plotted. The error bars show standard deviations. Only the difference in the chromatin-bound XPF between chromatin from control experiments & chromatin that was incubated one hour with 1 μM gemcitabine is statistically significant in HCT116 (p<0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30941207), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , , , , , , , , ,

Applications: , , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-116AF350
Alexa Fluor 405	NB100-116AF405
Alexa Fluor 488	NB100-116AF488
Alexa Fluor 532	NB100-116AF532
Alexa Fluor 594	NB100-116AF594
Alexa Fluor 647	NB100-116AF647
Alexa Fluor 700	NB100-116AF700
Alexa Fluor 750	NB100-116AF750
Allophycocyanin	NB100-116APC
Biotin	NB100-116B
CoraFluor 1	NB100-116CL1
DyLight 350	NB100-116UV
DyLight 405	NB100-116V
DyLight 488	NB100-116G
DyLight 550	NB100-116R
DyLight 594	NB100-116DL594
DyLight 650	NB100-116C
DyLight 680	NB100-116FR
DyLight 755	NB100-116IR
FITC	NB100-116F
HRP	NB100-116H
Janelia Fluor 525	NB100-116JF525
Janelia Fluor 549	NB100-116JF549
Janelia Fluor 646	NB100-116JF646
Janelia Fluor 669	NB100-116JF669
PE	NB100-116PE
PerCP	NB100-116PCP
mFluor Violet 450 SE	NB100-116MFV450
mFluor Violet 500 SE	NB100-116MFV500
mFluor Violet 610 SE	NB100-116MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-116SS
Azide and BSA Free	NBP2-80578

compare

(select up to 3 products from this page to compare)

NB110-58358

YAP1 Antibody - BSA Free

(2 Reviews)

(93 Publications)

0.1 ml

NB110-58358

Immunocytochemistry/Immunofluorescence: YAP1 Antibody - BSA Free [NB110-58358] - YAP/TAZ signaling is not activated by loss of TRAF3. NT, NF2 KO1, and TRAF3 KO1 cells stained for YAP1 and DAPI. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33185187/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: YAP1 Antibody - BSA Free [NB110-58358] - YAP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human YAP1 polyclonal Antibody (Catalog # NB110-58358) at 1:200 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclear and cytoplasm in trophoblast cells.

Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - Analysis in transfected HEK 293 cell lysate using YAP1 antibody. Observed molecular weight 75 kDa.

Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - Consequences of ORP5 and ORP8 knockdown on downstream MAPK and PI3K/AKT signaling. Protein from MOH parental, single and double ORP knockdowns as well as cells transfected with empty vector control (pLKO.1) were harvested, and 20 ug was subjected to SDS-PAGE and used for Western blotting. Image collected and cropped by CiteAb from the following publication (//www.life-science-alliance.org/lookup/doi/10.26508/lsa.201900431), licensed under a CC-BY license.

Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - YAP1 Antibody [NB110-58358] - Actomyosin activity inhibits beta-catenin- and YAP-driven proliferation of confluent keratinocytes. Effects of cell density and actomyosin activity on YAP phosphorylation. HaCaT cells cultured for 40 h under the sparse and confluent conditions were treated with 100 uM blebbistatin (Blebb) or DMSO (for control) for 6 h, and then lysed and immunoblotted for Ser127-phosphorylated YAP (pYAP), YAP, beta-catenin and actin. Similar results were obtained in two independent experiments. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep46326), licensed under a CC-BY license.

Simple Western: YAP1 Antibody - BSA Free [NB110-58358] - Simple Western lane view shows a specific band for YAP1 in 0.1 mg/ml of HeLa lysate. Observed molecular weight is 75 kDa. This experiment was performed under reducing conditions using the 12-230kDa separation system.

<b>Genetic Strategies Validation. </b>Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human YAP1 Polyclonal Antibody (Catalog # NB110-58358) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

<b>Genetic Strategies Validation. </b>Simple Western: YAP1 Antibody - BSA Free [NB110-58358] - Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A specific band was detected for YAP1 at approximately 81 kDa (as indicated) using 50 ug/mL of Rabbit Anti-YAP1 Polyclonal Antibody (Catalog # NB110-58358). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - Yap deficiency suppresses cell proliferation in vivo & in vitro. (A-F) The Ki67 positive ratio of lens epithelial cells decreased in Yap-deficient mice at different stages (arrowheads indicate Ki67 positive cells). (G) The relative number of Ki67 positive lens epithelial cells (number of Ki67 positive lens epithelial cells / lens epithelium area). The data are shown as mean ± S.E.M. (Student’s t-test, *P<0.05, **P<0.01, n=10). (H-I) Knockdown efficiency of Yap in alpha TN4 cell using siRNA. (J-K) Cell viability & growth assay revealed that proliferation was downregulated in Yap knockdown alpha TN4 cells. The data are shown as mean ± S.E.M. (Two-way RM ANOVA, **P<0.01, n=5). Scale bars: 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31011480), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - Synergistic effect of LW6 & metformin on YAP1. 80 µM LW6, 20 mM metformin (Met) & the combinational treatment metformin plus LW6 increased phosphorylation of YPA1 at serine 127 & decreased cellular YAP1 concentration after treating cells for 24 hours (A). Moreover, this combinational therapy attenuated the nuclear localization of YAP1 compared to Sham treated cells (B). In addition, lysophosphatidic acid (LPA) & the phosphorylation deficient mutant YAP1-S127A stimulate cell migration of 6606PDA cells (C & D). n = 2 per group for A, n = 3 per group for B, n = 7 per group for C, n = 9 per group for D. Bar = 5µm. Arrows point to nuclei. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31897243), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - Consequences of ORP5 & ORP8 knockdown on downstream MAPK & PI3K/AKT signaling.Protein from BxPC-3, PANC-1, MiaPaCa-2, & MOH parental, single & double ORP knockdowns as well as cells transfected with empty vector control (pLKO.1) were harvested, & 20 μg was subjected to SDS–PAGE & used for Western blotting. EGFR, MAPK, & PI3K signaling were assayed as pEGFR, ppERK, & pAKT levels, respectively. Amplification of YAP-1 was also evaluated. Total ERK, total AKT, & beta -actin levels were used as loading controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31451509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - Ajuba depletion induces beta -catenin translocation & Cyclin D1 expression in HCC cell lines. a Immunoblotting with specific antibodies against Ajuba, E-cadherin, beta -catenin, Cyclin D1, YAP, TAZ & CYR61 in Ajuba-depleted BEL7402 & HepG2 cells. GAPDH was used as a loading control. The ratios of expression E-Cadherin to their corresponding GAPDH are represented. b Ajuba-depleted HCC cells were fixed for immunofluorescence & stained for beta -catenin protein (green) & DAPI (blue). Representative merged images are also shown for fluorescence signals. Scale bar = 25 μm. c Correlation of Ajuba expression with OS in HCC. Low expression of Ajuba was associated with worse OS compared to high expression of Ajuba. Kaplan-Meier curves & log-rank test were used to evaluate OS. P < 0.05 was considered significant. d, e Representative images & quantification of migration & invasion of Ajuba-depleted (d) or Ajuba-overexpressing (e) HCC cells. Scale bar = 200 μm. HCC, hepatocellular carcinoma. f Expression in response to the overexpression of constructs of Ajuba-Myc was examined by IB, the ratios of expression Ajuba to their corresponding GAPDH are represented. Data are presented as Mean ± SEM from three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30041665), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: YAP1 Antibody - BSA Free [NB110-58358] - YAP & TAZ expression at high density & during chondrogenic differentiation of human synovial MSCs. (A) YAP & pYAP expression in human synovial membrane-derived (hSM-)MSCs in monolayer at low (L) & high (H) density detected by immunofluorescence staining, shown without (i) & with sytox green nuclear counterstain (ii). (B) YAP & pYAP expression in hSM-MSCs in monolayer at low (L) & high (H) density detected by western blotting with beta -actin as loading control. (C, D) Expression of YAP & TAZ (C) & their target genes CTGF & CYR61 (D) in hSM-MSCs immediately prior to (0 h) or 24 h after plating in micromass culture, determined by quantitative RT-PCR. Data was normalised to GAPDH expression, & is shown as mean ± standard deviation (SD) (three donors) relative to pre-seeding (0 h) control. *P <0.05; **P <0.01; ***P <0.001. (E) Expression of YAP & TAZ in hSM-MSCs after 6 days of treatment with 10 ng/ml TGF-beta 1 or vehicle only in micromass culture to induce chondrogenic differentiation, determined by quantitative RT-PCR. Data was normalised to GAPDH expression, & is shown as mean ± SD (five donors) relative to vehicle-treated control. *P <0.05. (F) Detection of YAP by western blotting during chondrogenic differentiation induced by TGF-beta 1 with detection of beta -actin as loading control. MSC, mesenchymal stromal/stem cell; pYAP, phosphorylated YAP; RT-PCR, reverse transcription PCR; TAZ, transcriptional co-activator with PDZ-binding motif; TGF, transforming growth factor; YAP, Yes-associated protein. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26025096), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: YAP1 Antibody - BSA Free [NB110-58358] - Expression of spcCre is associated with loss of YAP in SOX9+ distal airways.(A–D) Immunostaining of lung sections collected from Yapf/f; spcCre/+ mice at 13.5 days post coitus (dpc). SOX2 expression marks the proximal airway, while the distal airway is distinguished by SOX9 expression (not shown). High levels of spcCre expression were largely confined to the distal airway, where spcCre expression in a given epithelial cell was correlated with loss of YAP immunoreactivity (e.g. arrowheads in D). (E–H) Immunostaining of lung sections collected from Yapf/f; spcCre/+ mice at 14.5 dpc. Only distal airways are shown. YAP was lost mainly in distal airways in Yapf/f; spcCre/+ mice while sporadic loss of YAP was found in the proximal airway. Loss of YAP was most apparent in the more distal part (arrow in H) of the distal airway, while residual YAP could be found in the more proximal part of the distal airway. Together, these results suggest that lung cyst formation in distal airways of Yapf/f; spcCre/+ mice is due to loss of YAP in the distal airway. Scale bar = 25 μm for A–D; E–H.DOI://dx.doi.org/10.7554/eLife.21130.012 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28323616), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: YAP1 Antibody - BSA Free [NB110-58358] - The expression patterns of Yap & GFAP-Cre recombinase in postnatal mouse eyes. (A) Schematic of a transverse section of mouse eye. (B-C) Immunostaining with anti-Yap antibody (green) on frozen eye sections at different ages. Nuclei were counterstained with DAPI (blue). Yap staining was detected in scattered cells within the INL (arrowheads) & GCL of the retina & the lens epithelium (arrows). (D) Cre recombinase (red) was expressed in the lens epithelium & INL, GCL of retina in frozen eye sections of Tomatof/+; GFAP-Cre mice at P14. Nuclei were counterstained with DAPI (blue). LE, lens epithelium; TZ, transitional zone; RPE, retinal pigment epithelium; OS, outer segment; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars: 25 μm (B-C), 100 μm (D). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31011480), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: YAP1 Antibody - BSA Free [NB110-58358] - YAP & phospho-YAP are detected in both the proximal & distal airways during lung development.(A–H) Immunostaining of lung sections collected from wild-type mice at 13.5 days post coitus (dpc). The proximal airway is marked by SOX2 expression, while the distal airway is distinguished by SOX9 expression (not shown). Nuclear YAP can be frequently found in both SOX2+ & SOX9+ domains. Similarly, phospho-YAP at S112 (pYAP) could be detected in both the proximal & distal airways. pYAP levels were, in general, higher in the proximal than distal epithelium but pYAP levels varied significantly from cell to cell in both the proximal & distal airways. Representative cells with higher levels of pYAP (arrowhead) are indicated in (B,F). In many cells, low levels of pYAP were associated with the presence of nuclear YAP. This is consistent with a model in which pYAP is sequestered by 14-3-3 proteins in the cytoplasm & degraded but also indicate a dynamic shuttling & distribution of YAP along the entire airway epithelium. Similar results were obtained for lungs collected at 12.5 dpc. Scale bar = 7.5 μm for A–H.DOI://dx.doi.org/10.7554/eLife.21130.005 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28323616), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: YAP1 Antibody - BSA Free [NB110-58358] - Expression of Nkx2.1Cre is associated with loss of YAP in the upper lobes.(A–D) Immunostaining of lung sections collected from Yapf/f; Nkx2.1Cre/+ mice at 14.5 days post coitus (dpc). SOX2 expression marks the proximal airway, while the distal airway is distinguished by SOX9 expression (not shown). YAP was lost mainly in the upper lobe in Yapf/f; Nkx2.1Cre/+ lung. Loss of YAP was more apparent in the distal airway, while loss of YAP was sporadic in the proximal airway. Lung cyst formation was primarily observed in the distal airway. The boxed region in (B) indicates areas shown in (C,D). Scale bar = 250 μm for A,B; 250 μm for C,D. Sox2 expression was present in sporadic Yap-deficient cells in the transition zone induced by Sox9Cre, spcCre or Nkx2.1Cre. This suggests that Sox2 expression is not controlled by YAP.DOI://dx.doi.org/10.7554/eLife.21130.013 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28323616), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: YAP1 Antibody - BSA Free [NB110-58358] - Active nuclear YAP is distributed throughout the mouse lung epithelium during development.(A–P) Immunostaining of lung sections collected from wild-type mice at 11.5 & 12.5 days post coitus (dpc). The boxed region in (L) indicates areas shown in (N–P). The proximal airway is marked by SOX2 expression, while the distal airway is distinguished by SOX9 expression. Nuclear YAP can be frequently found in both SOX2+ & SOX9+ domains & is not restricted to the junction (the ‘transition zone’) between SOX2+ & SOX9+ domains. Representative cells with nuclear YAP (arrowhead) are indicated in (E,P). YAP immunoreactivity is completely absent in the epithelium (but present in the mesenchyme) of Yapf/f; ShhCre/+ mice (M), demonstrating the specificity of YAP antibodies used in this study. Immunofluorescence & immunohistochemistry yielded the same results (data not shown for immunohistochemistry). (Q–R) Whole-mount immunostaining of wild-type & Yap mutant lungs at 11.5 dpc. Distinct domains of SOX2 were discerned in the absence of YAP. Scale bar = 10 μm for A–J; 25 μm for K, L; 10 μm for N–P; 50 μm for Q, R.DOI://dx.doi.org/10.7554/eLife.21130.004 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28323616), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ca, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Ze

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-58358AF350
Alexa Fluor 532	NB110-58358AF532
Alexa Fluor 594	NB110-58358AF594
Alexa Fluor 750	NB110-58358AF750
Allophycocyanin	NB110-58358APC
DyLight 350	NB110-58358UV
DyLight 594	NB110-58358DL594
DyLight 680	NB110-58358FR
DyLight 755	NB110-58358IR
Janelia Fluor 549	NB110-58358JF549
Janelia Fluor 646	NB110-58358JF646
PE	NB110-58358PE
PerCP	NB110-58358PCP
mFluor Violet 450 SE	NB110-58358MFV450
mFluor Violet 500 SE	NB110-58358MFV500
mFluor Violet 610 SE	NB110-58358MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-58358SS

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NB100-101

APE Antibody - BSA Free

(3 Reviews)

(77 Publications)

0.1 ml

NB100-101

Immunohistochemistry: APE Antibody [NB100-101] - Immunohistochemical staining of APE-ref-1 in prostate cancer.

Western Blot: APE Antibody [NB100-101] - RKO cells were subjected to scrambled siRNA (Mock) or siRNA against TDP1 (TDP1KD) and cell lysates analyzed by immunoblotting (left). Control RKO (RKOSc siRNA) and RKO cells in which TDP1 levels were depleted (RKOTDP1 siRNA) were examined for their survival following exposure to the indicated doses of MMS, as described earlier in the text (right). Increasing the load of unrepaired methylated purines by exploiting the limited availability of TDP1 alone or in combination with canonical BER factors such as APE1 provides a new synthetic lethal setting to improve the clinical outcome of temozolomide-based cancer therapy. Image collected and cropped by CiteAb from the following publication (academic.oup.com/nar/article/42/5/3089/1051107), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: APE Antibody [NB100-101] - IF on HeLa cells. Image from verified customer review.

Western Blot: APE Antibody [NB100-101] - SH-SY5Y (nuclear extract) tested at 1:1000 dilution. Image provided by verified customer review.

Flow Cytometry: APE Antibody [NB100-101] - Spectral properties of Alexa Fluor(R) 488: an excitation maximum of 490 nm and an emission maximum of 525 nm. Use the Novus Spectra Viewer to identify the optimal laser(s) and filters for Alexa Fluor(R) 488 and to determine its compatibility with other fluorophores when designing a multicolor experiment. //www.novusbio.com/spectraviewer

Western Blot: APE Antibody [NB100-101] - Analysis of APE in human melanoma cell lysate using anti-APE antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: APE Antibody [NB100-101] - Detection of APE1 (Green) in HepG2 cells using NB100-101. Nuclei (Blue) are counterstained with Hoechst 33258.

Western Blot: APE Antibody - BSA Free [NB100-101] - Defective p53 activity leads to failure of the BER coordination system. (A) High-throughput microscopy analysis of TIG-1 cells transfected with plasmids expressing wild-type or mutant p53. Boxplots showing the distribution of APE1 staining intensity (in arbitrary units) in p53 low versus p53 overexpressing cells. Each p53 mutant is reported on top of the relevant plot. The dashed line highlights the median APE1 intensity in p53 low cells (N > 5000). NS: not statistically significant at P 8000). (D) Representative high-throughput immuno-fluorescence pictures showing TIG-1 cells stained for APE1 (right panel) & p53 (middle panel) after transfection with a p53 expressing plasmid. Cells that downregulating APE1 in response to p53 overexpression are marked by a contour line. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: APE Antibody - BSA Free [NB100-101] - Excessive AP-endonuclease activity in cells with p53 impairment leads to accumulation of genomic instability. (A) Representative western blotting analysis on WI38 (SV40) cells shows rescue of gamma H2AX staining upon co-depletion of XRCC1 & APE1. (B) Neutral Comet assay on WI38 (SV40) fibroblasts shows accumulation of DSBs upon XRCC1-depletion & rescue after co-depletion of XRCC1 & APE1. Results are expressed as mean ± SD of three independent experiments. (C) Representative western blotting analysis on WI38 (SV40) cells shows rescue of gamma H2AX staining after depletion of XRCC1 in presence of APE1 inhibitor III (APE1i III, 5 μM for 24 h), or AR03 (2.5 μM for 24 h). Actin was used as loading control in all western blotting experiments. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Pm, Rb

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-101AF350
Alexa Fluor 405	NB100-101AF405
Alexa Fluor 488	NB100-101AF488
Alexa Fluor 532	NB100-101AF532
Alexa Fluor 594	NB100-101AF594
Alexa Fluor 647	NB100-101AF647
Alexa Fluor 700	NB100-101AF700
Alexa Fluor 750	NB100-101AF750
Allophycocyanin	NB100-101APC
Biotin	NB100-101B
CoraFluor 1	NB100-101CL1
DyLight 350	NB100-101UV
DyLight 405	NB100-101V
DyLight 488	NB100-101G
DyLight 550	NB100-101R
DyLight 594	NB100-101DL594
DyLight 650	NB100-101C
DyLight 680	NB100-101FR
DyLight 755	NB100-101IR
FITC	NB100-101F
HRP	NB100-101H
Janelia Fluor 525	NB100-101JF525
Janelia Fluor 669	NB100-101JF669
PE	NB100-101PE
PerCP	NB100-101PCP
mFluor Violet 450 SE	NB100-101MFV450
mFluor Violet 500 SE	NB100-101MFV500
mFluor Violet 610 SE	NB100-101MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-101SS

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NB100-56503

Cytochrome c Antibody (7H8.2C12) - BSA Free

(62 Publications)

0.1 mg

NB100-56503

Flow (Intracellular): Cytochrome c Antibody (7H8.2C12) [NB100-56503] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Human HeLa lysate probed with Cytochrome C antibody at 0.1 ug/ml.

Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Analysis using the biotin conjugate of NB100-56503. Detection of Cytochrome C in 15 ug of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is detected.

Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Using the Alexa Fluor 488 direct conjugate, an intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Simple Western: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytochrome c at approximately 23 kDa (as indicated) using 2.5 ug/mL of Mouse Anti-Cytochrome c Monoclonal Antibody (Catalog # NB100-56503). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Cytochrome C Antibodies (NB100-56503SS), ProteinSimple Western Blot on Jess Instrument; 1 microgram of human brain tissue lysate was tested with the antibodies diluted 20 times. Image from verified customer review.

Effects of resveratrol on the expression of SIRT1, PGC-1 alpha , HIF-1 alpha & apoptosis proteins in vivo. (A) Activation of the SIRT1–PGC-1 alpha –HIF-1 alpha signaling pathway in PC–AKI associated with DN in rabbits. Representative western blot images of proteins in the rabbits with DN treated with saline (Cont), resveratrol alone (Res), iohexol (PC–AKI), & co-treatment with resveratrol & iohexol (Res+PC–AKI). (B–H) Relative densitometry analysis of the ratios of SIRT1–PGC-1 alpha –HIF-1 alpha signaling proteins to beta -actin were expressed as mean ± standard error. *P < 0.05 vs. Cont; #P < 0.05 vs. PC–AKI. Cont, control; Res, resveratrol; PC–AKI, post-contrast acute kidney injury; SIRT1, silent information regulator l; HIF-1 alpha , hypoxia-inducible transcription factor-1 alpha ; DN, diabetic nephropathy; PGC-1 alpha , peroxisome proliferator-activated receptor gamma coactivator-1 alpha. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31402864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Dr, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Dr, Rb

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-101A

Alt Catalog# IMG-101A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-56503AF350
Alexa Fluor 405	NB100-56503AF405
Alexa Fluor 488	NB100-56503AF488
Alexa Fluor 532	NB100-56503AF532
Alexa Fluor 594	NB100-56503AF594
Alexa Fluor 647	NB100-56503AF647
Alexa Fluor 700	NB100-56503AF700
Alexa Fluor 750	NB100-56503AF750
Allophycocyanin	NB100-56503APC
Biotin	NB100-56503B
Biotin	NB100-55775
DyLight 350	NB100-56503UV
DyLight 405	NB100-56503V
DyLight 488	NB100-56503G
DyLight 550	NB100-56503R
DyLight 594	NB100-56503DL594
DyLight 650	NB100-56503C
DyLight 680	NB100-56503FR
DyLight 755	NB100-56503IR
FITC	NB100-56503F
HRP	NB100-56503H
Janelia Fluor 549	NB100-56503JF549
Janelia Fluor 646	NB100-56503JF646
PE	NB100-56503PE
PerCP	NB100-56503PCP
mFluor Violet 450 SE	NB100-56503MFV450
mFluor Violet 500 SE	NB100-56503MFV500
mFluor Violet 610 SE	NB100-56503MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56503SS
Azide and BSA Free	NBP2-80686

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NB110-93479

Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free

(5 Reviews)

(60 Publications)

0.05 ml

NB110-93479

<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] antibody (1:250) was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Detection of PDHE1 alpha [S293] in an in vitro autophosphorylation of PDH complex in response to different stimulants: (-) none, H202-hydrogen peroxide, AR-anhydroretinol, Rol-retinol, RA-retinoic acid, PMA, Lipid-PKC lipid activator. Photo courtesy of Dr. Beatrice Hoyos, Memorial Sloan-Kettering Cancer Center.

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - PDH activity and protein levels of PDK4 and p-PDH in hindlimb skeletal muscle. Samples were resolved by electrophoresis, transferred and probed by antibodies and representative immunoblots and normalization of pPDH are presented. The following comparisons were made: within a genotype, fed versus fasted; across genotypes, fed versus fed and fasted versus fasted. Bars that do not share a common symbol differ significantly (P < 0.05). Data presented as mean +/- SEM (n = 6). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0124204), licensed under a CC-BY license.

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free [NB110-93479] - PDK4 expression during syncytialization of human placental trophoblasts. (A) Changes of the RPKM values for PDK family members in trophoblasts before (red column, 3 hours) & after (blue column, 48 hours) syncytialization. (B) Changes in PDK4 mRNA (black column, n = 4) & protein (white column, n = 4) abundance during syncytialization. (C) Changes in the phosphorylation of PDHE1 alpha during syncytialization n = 4. (D) Representative images showing intense staining of PDK4 (red) in the cytotrophoblast layer & weak staining of PDK4 in the syncytial layer of human chorionic villi at early gestation. The syncytial & cytotrophoblast layers were labeled with beta -hCG (green) & SPINT1 (green) respectively. Nuclei were counterstained with DAPI (blue). n = 3; *P < 0.05; **P < 0.01; ***P < 0.001 against 3 hours; n.s., not significant. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-09163-8), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 405	NB110-93479AF405
Alexa Fluor 488	NB110-93479AF488
Alexa Fluor 647	NB110-93479AF647
Alexa Fluor 700	NB110-93479AF700
Biotin	NB110-93479B
DyLight 405	NB110-93479V
DyLight 488	NB110-93479G
DyLight 550	NB110-93479R
DyLight 650	NB110-93479C
FITC	NB110-93479F
HRP	NB110-93479H

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-93479SS

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H00010413-M01

YAP1 Antibody (2F12) - Isoform 5 - Azide and BSA Free

(65 Publications)

0.1 mg

H00010413-M01

Immunocytochemistry/Immunofluorescence: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of monoclonal antibody to YAP1 on HeLa cell. Antibody concentration 30 ug/ml.

Immunohistochemistry-Paraffin: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of monoclonal antibody to YAP1 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 3 ug/ml.

Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - JNK specifically phosphorylates YAP in vivo. HaCaT, U2OS, MCF7 and 293T cell lines were treated with anisomycin or DMSO (control) for 60 min before harvesting. YAP band shift was analyzed as described before and lysates were analyzed by Western blotting with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis20107) licensed under a CC-BY license.

Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - Immunogen detection using YAP1 antibody (2F12) Isoform 5, observered MW 37.73KDa) .

Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of YAP1 Antibody (2F12) Isoform 5 in transfected 293T cell line. Lane 1: YAP1 transfected lysate (TMW: 54.5 KDa, observed MW 75 KDa). Lane 2: Non-transfected lysate.

Reactivity:

Hu, Mu, Po, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Po, Bv, Ca

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB600-302

c-Myc Antibody (9E10) - BSA Free

(4 Reviews)

(48 Publications)

0.1 ml

NB600-302

Simple Western: c-Myc Antibody (9E10) [NB600-302] - Simple Western lane view shows a specific band for c-Myc in 0.5 mg/ml of Jurkat lysate. This experiment as performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: c-Myc Antibody (9E10) - BSA Free [NB600-302] - Western blotting analysis of unsorted (W) and sorted ROSl degrees w(L) and ROShigh(H) cells. Data shown are representative of at least two independent experiments.DyLight 650 conjugated version of this antibody used (NB600-302C). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25857523/) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: c-Myc Antibody (9E10) [NB600-302] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc (9E10) at 10 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow (Intracellular): c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.. Using the PE format of this antibody.

Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302.

Western Blot: c-Myc Antibody (9E10) [NB600-302] - Flow cytometric analysis of ROS and the mitochondrial status. Western blotting analysis of unsorted (W) and sorted P1 and P2 cells. Data shown are representative of at least two independent experiments. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/ncomms7750) licensed under a CC-BY license.

Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

Flow (Intracellular): c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.. Using the FITC format of this antibody.

Western Blot: c-Myc Antibody (9E10) - BSA Free [NB600-302] - NLRP2 interacts with SCMC components TLE6, OOEP, FILIA & NLRP5.(A) NLRP2 was overexpressed with myc-tagged OOEP, TLE6, NLRP5 & FILIA in HEK293T cells for 48 hours, immunoprecipitated with anti-NLRP2 or IgG as negative control & immunoblotted with anti-myc. Top panel shows specificity of anti-NLRP2 IP & bottom panel shows that NLRP2 binds to OOEP, TLE6, FILIA & NLRP5. Uncropped, full length western blots have been provided in Supplementary Figure 5. (B) Whole-mount immunofluorescence co-staining with anti-NLRP2 (green) & DAPI (blue) for nuclear staining on Nlrp2+/+ oocytes & embryos at 2-, 4-,16-cell & morula stages revealed a predominantly SCMC-like localization for NLRP2. Scale bars represent 200 μm. (C) Co-staining with anti-TLE6 (green) & DAPI (blue) of paraffin-embedded oocyte sections reveals a typical cortical stain of TLE6 in oocytes of Nlrp2+/+ dams but a more intense & diffuse stain in oocytes of Nlrp2tm1a/tm1a dams. Scale bars represent 10 μm. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep44667), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Bv, Dr

Species Glossary

Reactivity: Hu, Mu, Bv, Dr

Applications:

, , , , , , , , ,

Applications: , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-302AF350
Alexa Fluor 405	NB600-302AF405
Alexa Fluor 488	NB600-302AF488
Alexa Fluor 532	NB600-302AF532
Alexa Fluor 594	NB600-302AF594
Alexa Fluor 647	NB600-302AF647
Alexa Fluor 700	NB600-302AF700
Alexa Fluor 750	NB600-302AF750
Allophycocyanin	NB600-302APC
Biotin	NB600-302B
CoraFluor 1	NB600-302CL1
DyLight 350	NB600-302UV
DyLight 405	NB600-302V
DyLight 488	NB600-302G
DyLight 550	NB600-302R
DyLight 594	NB600-302DL594
DyLight 650	NB600-302C
DyLight 680	NB600-302FR
DyLight 755	NB600-302IR
FITC	NB600-302F
HRP	NB600-302H
Janelia Fluor 525	NB600-302JF525
Janelia Fluor 549	NB600-302JF549
Janelia Fluor 646	NB600-302JF646
Janelia Fluor 669	NB600-302JF669
PE	NB600-302PE
PerCP	NB600-302PCP
mFluor Violet 450 SE	NB600-302MFV450
mFluor Violet 500 SE	NB600-302MFV500
mFluor Violet 610 SE	NB600-302MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-302SS
Azide and BSA Free	NBP2-80675

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NB600-335

c-Myc Antibody - BSA Free

(1 Review)

(59 Publications)

0.1 ml

NB600-335

Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.4 ug/mL (1:25,000).

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Agarose	NB600-342
Alexa Fluor 350	NB600-335AF350
Alexa Fluor 405	NB600-335AF405
Alexa Fluor 488	NB600-335AF488
Alexa Fluor 532	NB600-335AF532
Alexa Fluor 594	NB600-335AF594
Alexa Fluor 647	NB600-335AF647
Alexa Fluor 700	NB600-335AF700
Alexa Fluor 750	NB600-335AF750
Allophycocyanin	NB600-335APC
Biotin	NB600-335B
CoraFluor 1	NB600-335CL1
DyLight 350	NB600-335UV
DyLight 405	NB600-335V
DyLight 488	NB600-335G
DyLight 550	NB600-335R
DyLight 594	NB600-335DL594
DyLight 650	NB600-335C
DyLight 680	NB600-335FR
DyLight 755	NB600-335IR
FITC	NB600-335F
HRP	NB600-335H
HRP	NB600-341
Janelia Fluor 525	NB600-335JF525
Janelia Fluor 549	NB600-335JF549
Janelia Fluor 646	NB600-335JF646
Janelia Fluor 669	NB600-335JF669
PE	NB600-335PE
PerCP	NB600-335PCP

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NB100-338

NDC80 Antibody (9G3.23) - Azide and BSA Free

(5 Reviews)

(49 Publications)

0.1 ml

NB100-338

Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - Mouse anti-Hec1 and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.

Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in fixed human cell lines of RPE1, H4, and 42MGBA. Image courtesy of anonymous customer product review.

Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in PTK1 cells.Verified customer review from 1DegreeBio.

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Blue: Fluoroshield with DAPI .

<b>Orthogonal Strategies Validation. </b>Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Reactivity:

Hu, Mu, Ha, Mar

Species Glossary

Reactivity: Hu, Mu, Ha, Mar

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB200-103

p53 Antibody (PAb 240) - BSA Free

(3 Reviews)

(43 Publications)

0.1 ml

NB200-103

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 Antibody [PAb 240] NB200-103 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - Neuro2a cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on MCF7 cells with p53 [PAb 240] Antibody NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on Neuro2a cells with p53 Antibody [PAb 240] NB200-103 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Reactivity:

Hu, Mu, Rt, Ye, Xp(-)

Species Glossary

Reactivity: Hu, Mu, Rt, Ye, Xp(-)

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB200-103AF350
Alexa Fluor 405	NB200-103AF405
Alexa Fluor 488	NB200-103AF488
Alexa Fluor 532	NB200-103AF532
Alexa Fluor 594	NB200-103AF594
Alexa Fluor 647	NB200-103AF647
Alexa Fluor 700	NB200-103AF700
Alexa Fluor 750	NB200-103AF750
Allophycocyanin	NB200-103APC
Biotin	NB200-103B
CoraFluor 1	NB200-103CL1
DyLight 350	NB200-103UV
DyLight 405	NB200-103V
DyLight 488	NB200-103G
DyLight 550	NB200-103R
DyLight 594	NB200-103DL594
DyLight 650	NB200-103C
DyLight 680	NB200-103FR
DyLight 755	NB200-103IR
FITC	NB200-103F
HRP	NB200-103H
Janelia Fluor 525	NB200-103JF525
Janelia Fluor 549	NB200-103JF549
Janelia Fluor 646	NB200-103JF646
Janelia Fluor 669	NB200-103JF669
PE	NB200-103PE
PerCP	NB200-103PCP
mFluor Violet 450 SE	NB200-103MFV450
mFluor Violet 500 SE	NB200-103MFV500
mFluor Violet 610 SE	NB200-103MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB200-103SS
Azide and BSA Free	NBP2-80898

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NB110-37235

SOX2 Antibody - BSA Free

(4 Reviews)

(38 Publications)

0.1 ml

NB110-37235

Flow Cytometry: SOX2 Antibody [NB110-37235] - An intracellular stain was performed on A549 with NB110-37235 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylgiht 550.

Western Blot: SOX2 Antibody [NB110-37235] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and immunoblot assay was performed. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (NB110-37235) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235] - An intracellular stain was performed on U-251 MG cells with SOX2 Antibody NB110-37235 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Western Blot: SOX2 Antibody [NB110-37235] - Expression of pluripotency factor Sox2 in human head and neck cancer cell lines FaDu and HN5. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - Staining of SOX2 in NTERA2 cells with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.

Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-SOX2 (NB110-37235) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (NB110-37235) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Reactivity:

Hu, Mu, Rt, Ca, Sh, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Sh, Ch

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-37235AF350
Alexa Fluor 405	NB110-37235AF405
Alexa Fluor 488	NB110-37235AF488
Alexa Fluor 532	NB110-37235AF532
Alexa Fluor 594	NB110-37235AF594
Alexa Fluor 647	NB110-37235AF647
Alexa Fluor 700	NB110-37235AF700
Alexa Fluor 750	NB110-37235AF750
Allophycocyanin	NB110-37235APC
Biotin	NB110-37235B
DyLight 350	NB110-37235UV
DyLight 405	NB110-37235V
DyLight 488	NB110-37235G
DyLight 550	NB110-37235R
DyLight 594	NB110-37235DL594
DyLight 650	NB110-37235C
DyLight 680	NB110-37235FR
DyLight 755	NB110-37235IR
FITC	NB110-37235F
HRP	NB110-37235H
Janelia Fluor 549	NB110-37235JF549
Janelia Fluor 646	NB110-37235JF646
PE	NB110-37235PE
PerCP	NB110-37235PCP
mFluor Violet 450 SE	NB110-37235MFV450
mFluor Violet 500 SE	NB110-37235MFV500
mFluor Violet 610 SE	NB110-37235MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-37235SS

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

DNMT1 Antibody (60B1220.1) - BSA Free

APE Antibody (13B8E5C2) - BSA Free

YAP1 Antibody - BSA Free

APE Antibody - BSA Free

Cytochrome c Antibody (7H8.2C12) - BSA Free

Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free

YAP1 Antibody (2F12) - Isoform 5 - Azide and BSA Free

c-Myc Antibody (9E10) - BSA Free

c-Myc Antibody - BSA Free

NDC80 Antibody (9G3.23) - Azide and BSA Free

p53 Antibody (PAb 240) - BSA Free

SOX2 Antibody - BSA Free