Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NB100-97821] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1B antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NB100-97821] - MCF-7 cells stained with Lysine (K)-specific Demethylase 5B antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NB100-97821] - Section of human prostate carcinoma. Antibody: Rabbit anti-JARID1B antibody used at 1:500 (2ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Immunoprecipitation: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NB100-97821] - Detection of human JARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JARID1B antibody NB100-97821 (lot NB100-97821-3) used for IP at 6 ug per reaction. JARID1B was also immunoprecipitated by a previous lot of this antibody (lot NB100-97821-2). For blotting immunoprecipitated Jarid1B, NB100-97821 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1494 and 1544 of human Jumonji, AT rich interactive domain 1B (NP_006609.3).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM5B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Flow reactivity reported in (PMID: 30721788). Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B antibody validated for IHC-P from a verified customer review.
• Control:

  Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

• CT31
• CT31FLJ10538
• EC 1.14.11
• EC 1.14.11.-
• homolog 1A
• Jarid1B
• JARID1BFLJ16281
• Jumonji, AT rich interactive domain 1B (RBP2-like)
• jumonji, AT rich interactive domain 1B
• KDM5B
• Lysine (K)specific Demethylase 5B
• Lysine (K)-specific Demethylase 5B
• PLU-1
• PLU-1FLJ12459
• PLU1FLJ23670
• PUT1
• RBBP2H1
• RBBP2H1AFLJ12491
• RBP2-H1
• Retinoblastoma-binding protein 2 homolog 1

Background

JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (NB100-97821)(10)

Publications using NB100-97821

• Webster MR, Fane ME, Alicea GM Paradoxical Role for Wild-Type p53 in Driving Therapy Resistance in Melanoma Mol. Cell 2019-12-11 [PMID: 31836388]
• Vogel FCE, Bordag N, Zugner E et al. Targeting the H3K4 demethylase KDM5B reprograms the metabolome and phenotype of melanoma cells J. Invest. Dermatol. 2019-06-20 [PMID: 31229500]
• Shannan B, Matschke J, Chauvistre H et al. Sequence-dependent cross-resistance of combined radiotherapy plus BRAFV600E inhibition in melanoma Eur. J. Cancer 2019-03-01 [PMID: 30721788] (FLOW, Human)
• Boyle SE, Fedele CG, Corbin V et al. CD271 Expression on Patient Melanoma Cells Is Unstable and Unlinked to Tumorigenicity. Cancer Res. 2016-06-20 [PMID: 27325642] (IHC, Human)
• Lu W, Liu S, Li B et al. SKP2 inactivation suppresses prostate tumorigenesis by mediating JARID1B ubiquitination. Oncotarget 2015-01-20 [PMID: 25596733] (IHC)
• Stanisz H, Saul S, Muller CS et al. Inverse regulation of melanoma growth and migration by Orai1/STIM2-dependent calcium entry. Pigment Cell Melanoma Res 2014-01-29 [PMID: 24472175] (WB, Human)
• Roesch A, Vultur A, Bogeski I et al. Overcoming Intrinsic Multidrug Resistance in Melanoma by Blocking the Mitochondrial Respiratory Chain of Slow-Cycling JARID1Bhigh Cells. Cancer Cell 2013-06-10 [PMID: 23764003] (WB, Human)
• Chauvistre H, Daignault SM, Shannan B, Ju RJ The Janus-faced role of KDM5B heterogeneity in melanoma: differentiation as a situational driver of both growth arrest and drug-resistance bioRxiv 2020-01-01 (ICC/IF, Human)
• Zhang S, Cai W, Liu X et al. Ablation of Histone Demethylase KDM5B in Melanoma Augments Anti-Tumor Immunity through Upregulation of Retroelements Research Square 2020-11-06 (ChIP, IP)
• Zhang SM, Cai WL, Liu X Et al. KDM5B promotes immune evasion by recruiting SETDB1 to silence retroelements Nature 2021-10-01 [PMID: 34671158] (ChIP)

Review for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (NB100-97821) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Emily Beirne IHC-P Human 10/06/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: MCF7
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: KDM5B
• Entrez:
  • 10765(Human)
  • 75605(Mouse)
• Uniprot:
  • Q9UGL1()