Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

Images

 
Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NB100-97821] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NB100-97821] - MCF-7 cells stained with Lysine (K)-specific Demethylase 5B antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NB100-97821] - Section of human prostate carcinoma. Antibody: Rabbit anti-JARID1B antibody used at 1:500 (2ug/ml). Secondary: ...read more
Immunoprecipitation: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NB100-97821] - Detection of human JARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1494 and 1544 of human Jumonji, AT rich interactive domain 1B (NP_006609.3).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM5B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Flow Cytometry
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Flow reactivity reported in (PMID: 30721788). Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B antibody validated for IHC-P from a verified customer review.
Control
Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-97821 in the following applications:

Publications
Read Publications using
NB100-97821 in the following applications:

  • 1 publication
  • 1 publication
  • 1 publication
  • IHC
    2 publications
  • IP
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

  • CT31
  • CT31FLJ10538
  • EC 1.14.11
  • EC 1.14.11.-
  • homolog 1A
  • Jarid1B
  • JARID1BFLJ16281
  • Jumonji, AT rich interactive domain 1B (RBP2-like)
  • jumonji, AT rich interactive domain 1B
  • KDM5B
  • Lysine (K)specific Demethylase 5B
  • Lysine (K)-specific Demethylase 5B
  • PLU-1
  • PLU-1FLJ12459
  • PLU1FLJ23670
  • PUT1
  • RBBP2H1
  • RBBP2H1AFLJ12491
  • RBP2-H1
  • Retinoblastoma-binding protein 2 homolog 1

Background

JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (NB100-97821)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: CHIP, Flow, ICC/IF, IHC, IP, WB.


Filter By Application
CHIP
(1)
Flow
(1)
ICC/IF
(1)
IHC
(2)
IP
(1)
WB
(2)
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Filter By Species
Human
(5)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-97821 Applications Species
Vogel FCE, Bordag N, Zugner E et al. Targeting the H3K4 demethylase KDM5B reprograms the metabolome and phenotype of melanoma cells J. Invest. Dermatol. Jun 20 2019 [PMID: 31229500]
Shannan B, Matschke J, Chauvistre H et al. Sequence-dependent cross-resistance of combined radiotherapy plus BRAFV600E inhibition in melanoma Eur. J. Cancer Mar 1 2019 [PMID: 30721788] (Flow, Human) Flow Human
Boyle SE, Fedele CG, Corbin V et al. CD271 Expression on Patient Melanoma Cells Is Unstable and Unlinked to Tumorigenicity. Cancer Res. Jun 20 2016 [PMID: 27325642] (IHC, Human) IHC Human
Lu W, Liu S, Li B et al. SKP2 inactivation suppresses prostate tumorigenesis by mediating JARID1B ubiquitination. Oncotarget 2015 Jan 20 [PMID: 25596733] (IHC) IHC
Stanisz H, Saul S, Muller CS et al. Inverse regulation of melanoma growth and migration by Orai1/STIM2-dependent calcium entry. Pigment Cell Melanoma Res 2014 Jan 29 [PMID: 24472175] (WB, Human) WB Human
Roesch A, Vultur A, Bogeski I et al. Overcoming Intrinsic Multidrug Resistance in Melanoma by Blocking the Mitochondrial Respiratory Chain of Slow-Cycling JARID1Bhigh Cells. Cancer Cell 2013 Jun 10 [PMID: 23764003] (WB, Human) WB Human
Chauvistre H, Daignault SM, Shannan B, Ju RJ The Janus-faced role of KDM5B heterogeneity in melanoma: differentiation as a situational driver of both growth arrest and drug-resistance bioRxiv Jan 1 2020 (ICC/IF, Human) ICC/IF Human
Webster MR, Fane ME, Alicea GM Paradoxical Role for Wild-Type p53 in Driving Therapy Resistance in Melanoma Mol. Cell Dec 11 2019 [PMID: 31836388]
Zhang S, Cai W, Liu X et al. Ablation of Histone Demethylase KDM5B in Melanoma Augments Anti-Tumor Immunity through Upregulation of Retroelements Research Square Nov 6 2020 (IP, CHIP) IP, CHIP

Review for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (NB100-97821) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-97821:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B NB100-97821
Enlarge
5
reviewed by:
Anonymous
IHC-P Human 10/06/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMCF7
SpeciesHuman
LotA1

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-97821AF350
Alexa Fluor 405 NB100-97821AF405
Alexa Fluor 488 NB100-97821AF488
Alexa Fluor 532 NB100-97821AF532
Alexa Fluor 594 NB100-97821AF594
Alexa Fluor 647 NB100-97821AF647
Alexa Fluor 700 NB100-97821AF700
Alexa Fluor 750 NB100-97821AF750
Biotin NB100-97821B
DyLight 350 NB100-97821UV
DyLight 405 NB100-97821V
DyLight 488 NB100-97821G
DyLight 550 NB100-97821R
DyLight 594 NB100-97821DL594
DyLight 650 NB100-97821C
DyLight 680 NB100-97821FR
DyLight 755 NB100-97821IR
FITC NB100-97821F
HRP NB100-97821H
Janelia Fluor 549 NB100-97821JF549
Janelia Fluor 646 NB100-97821JF646

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
10/06/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol KDM5B
Entrez
Uniprot