Human HE4/WFDC2 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human HE4/WFDC2 Quantikine ELISA Kit Summary

• Background: The Quantikine Human HE4/WFDC2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human HE4/WFDC2 in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human HE4/WFDC2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human HE4/WFDC2 showed linear curves that were parallel to the stand...ard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human HE4/WFDC2.
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• Specificity: Natural and recombinant human HE4/WFDC2
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: WFDC2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human HE4/WFDC2 Quantikine ELISA Kit

• dJ461P17.6
• EDDM4
• Epididymal secretory protein E4
• epididymis-specific, whey-acidic protein type, four-disulfide core
• HE4
• HE4MGC57529
• Major epididymis-specific protein E4
• Putative protease inhibitor WAP5
• WAP domain containing protein HE4-V4
• WAP four-disulfide core domain 2
• WAP four-disulfide core domain protein 2
• WAP5
• WAP5epididymal protein 4
• WFDC2

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for HE4/WFDC2 (DHE400)(8)

Publications using DHE400

• L Ning, J Lang, B Long, L Wu Diagnostic value of circN4BP2L2 in type I and type II epithelial ovarian cancer BMC Cancer, 2022;22(1):1210. 2022 [PMID: 36434559] (Human)
• L Ning, J Lang, L Wu Plasma circN4BP2L2 is a promising novel diagnostic biomarker for epithelial ovarian cancer BMC Cancer, 2022;22(1):6. 2022 [PMID: 34980005] (Human)
• Q Jin, Y Tang, Z Liu, W Xie, Q Luo, Z Zhao, Q Zhao, Z Huang, X Yu, L Yan, C Xiong, X Ni, Y Yan Serum human epididymis protein 4 level as a predictor of clinical worsening in idiopathic pulmonary arterial hypertension: a pilot study BMC Cardiovasc Disord, 2020;20(1):175. 2020 [PMID: 32295533] (Human)
• KK Kim, RK Singh, N Khazan, A Kodza, NA Singh, A Jones, U Sivagnanal, M Towner, H Itamochi, R Turner, RG Moore Development of Potent Forchlorfenuron Analogs and Their Cytotoxic Effect in Cancer Cell Lines Sci Rep, 2020;10(1):3241. 2020 [PMID: 32094384] (Human)
• K Parikh, A Antanavici, D Fawkner-Co, M Jagielowic, A Aulicino, C Lagerholm, S Davis, J Kinchen, HH Chen, NK Alham, N Ashley, E Johnson, P Hublitz, L Bao, J Lukomska, RS Andev, E Björklund, BM Kessler, R Fischer, R Goldin, H Koohy, A Simmons Colonic epithelial cell diversity in health and inflammatory bowel disease Nature, 2019;0(0):. 2019 [PMID: 30814735] (Human)
• RM Williams, C Lee, TV Galassi, JD Harvey, R Leicher, M Sirenko, MA Dorso, J Shah, N Olvera, F Dao, DA Levine, DA Heller Noninvasive ovarian cancer biomarker detection via an optical nanosensor implant Sci Adv, 2018;4(4):eaaq1090. 2018 [PMID: 29675469] (Human)
• Huang , Tianhe, Jiang , Shi-Wen, Qin , Liangyi, Senkowski , Christop, Lyle , Christia, Terry , Karen, Brower , Steven, Chen , Haibin, Glasgow , Wayne, Wei , Yongchan, Li , Jinping Expression and diagnostic value of HE4 in pancreatic adenocarcinoma. Int J Mol Sci, 2015;16(2):2956-70. 2015 [PMID: 25642754] (Human)
• Tamir , Ayala, Jag , Ushma, Sarojini , Sreeja, Schindewolf , Craig, Tanaka , Takemi, Gharbaran , Rajendra, Patel , Hiren, Sood , Anil, Hu , Wei, Patwa , Ruzeen, Blake , Patrick, Chirina , Polina, Oh Jeong , Jin, Lim , Heejin, Goy , Andre, Pecora , Andrew, Suh , K Stephe Kallikrein family proteases KLK6 and KLK7 are potential early detection and diagnostic biomarkers for serous and papillary serous ovarian cancer subtypes. J Ovarian Res, 2014;7(1):109. 2014 [PMID: 25477184] (Human)

FAQs for HE4/WFDC2 (DHE400). (Showing 1 - 1 of 1 FAQs).

1. I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?
   • At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following link: www.novusbio.com/support/innovators-reward.html

Bioinformatics

• Gene Symbol: WFDC2