ZEB2 Antibody (CL13467) - Azide and BSA Free

Staining of human anaplastic oligodendroglioma shows strong nuclear positivity in tumor cells.

Staining in U-87 cell line with Anti-ZEB2 monoclonal antibody) .

Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-43869 antibody. Corresponding ZEB2 RNA-seq data are presented for the same tissues.

Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human tonsil shows strong nuclear positivity in germinal center cells.

Analysis in human cell lines SK-MEL-30 and Caco-2 using Anti-ZEB2 antibody. Corresponding ZEB2 RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL13467
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ZEB2 Antibody (CL13467) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ZEB2 Antibody (CL13467) - Azide and BSA Free (NBP3-24559) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of O60315, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZEB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For Immunocytochemistry/ Immunofluorescence/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for ZEB2 Antibody (CL13467) - Azide and BSA Free

• HSPC082
• KIAA0569FLJ42816
• SIP1
• SIP1HSPC082
• Smad-interacting protein 1
• SMADIP1
• SMADIP1ZFHX1BSIP-1
• ZEB2
• ZFHX1B
• ZFX1B
• zinc finger E-box binding homeobox 2
• zinc finger E-box-binding homeobox 2
• zinc finger homeobox 1b
• Zinc finger homeobox protein 1b

Background

The ZEB2 gene (also known as SIP1 and SMADIP1) is a member of the delta-EF1 (ZEB1; MIM 189909)/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SMADIP1 interacts with receptor-mediated, activated full-length SMADs (see MIM 605568) (Verschueren et al., 1999 [PubMed 10400677]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZEB2