ZBTB20 Antibody - BSA Free

Western Blot: ZBTB20 Antibody [NBP2-20936] - HeLa whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZBTB20 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: ZBTB20 Antibody [NBP2-20936] - DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZBTB20 stained by ZBTB20 antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: ZBTB20 Antibody [NBP2-20936] - Mouse hippocampus. ZBTB20 stained by ZBTB20 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: ZBTB20 Antibody [NBP2-20936] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: ZBTB20 Antibody [NBP2-20936] - ZBTB20 antibody detects ZBTB20 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZBTB20 stained by ZBTB20 antibody diluted at 1:500. Red: Tau, stained by Tau antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: ZBTB20 Antibody [NBP2-20936] - Human breast cancer, using ZBTB20 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ZBTB20 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ZBTB20 Antibody - BSA Free (NBP2-20936) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ZBTB20 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ZBTB20. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZBTB20
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated Reported in scientific publication (PMID: 32363359)
  • Western Blot 1:500-1:3000
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for ZBTB20 Antibody - BSA Free

• BTB/POZ zinc finger protein DPZF
• dendritic cell-derived BTB/POZ zinc finger
• Dendritic-derived BTB/POZ zinc finger protein
• DPZFZinc finger protein 288ZNF288DKFZp566F123
• FLJ26458
• HOF
• ODA-8S
• zinc finger and BTB domain containing 20
• zinc finger and BTB domain-containing protein 20

Background

ZBTB20 - zinc finger and BTB domain containing 20

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ZBTB20 Antibody (NBP2-20936)(1)

Publication using NBP2-20936

• Qiu J, Peng P, Xin M et al. ZBTB20-mediated titanium particle-induced peri-implant osteolysis by promoting macrophage inflammatory responses Biomater Sci 2020-06-07 [PMID: 32363359] (WB, IF/IHC, KD, Human)

Bioinformatics

• Gene Symbol: ZBTB20